Restriction map for
pF1KB7695
(Circular) (Six-base) MAPSORT of: pF1KB7695.seq Check: 2941 from: 1 to: 4274
pF1KB7695 4274 bp
With 182 enzymes: SgfI *
June 26, 2008 10:49 ..
AccI GT'mk_AC
Cuts at: 1312 1312
Size: 4274
Acc65I G'GTAC_C
Cuts at: 1290 1290
Size: 4274
AclI AA'CG_TT
Cuts at: 4114 4114
Size: 4274
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 442 523 1255 2099 2531 3278 3546 442
Size: 81 732 844 432 747 268 1170
Fragments arranged by size:
1170 844 747 732 432 268 81
AflIII A'CryG_T
Cuts at: 320 1160 1626 2730 3707 320
Size: 840 466 1104 977 887
Fragments arranged by size:
1104 977 887 840 466
AgeI A'CCGG_T
Cuts at: 2662 2662
Size: 4274
AhdI GACnn_n'nnGTC
Cuts at: 962 962
Size: 4274
AlwNI CAG_nnn'CTG
Cuts at: 389 546 1128 1579 3146 389
Size: 157 582 451 1567 1517
Fragments arranged by size:
1567 1517 582 451 157
ApaLI G'TGCA_C
Cuts at: 3044 3044
Size: 4274
ApoI r'AATT_y
Cuts at: 1278 3627 3883 1278
Size: 2349 256 1669
Fragments arranged by size:
2349 1669 256
AseI AT'TA_AT
Cuts at: 20 20
Size: 4274
AsiSI GCG_AT'CGC
Cuts at: 104 104
Size: 4274
AvaI C'yCGr_G
Cuts at: 306 457 565 967 1294 306
Size: 151 108 402 327 3286
Fragments arranged by size:
3286 402 327 151 108
AvrII C'CTAG_G
Cuts at: 138 138
Size: 4274
BamHI G'GATC_C
Cuts at: 1299 1299
Size: 4274
BanI G'GyrC_C
Cuts at: 894 1229 1290 1704 1952 1987 894
Size: 335 61 414 248 35 3181
Fragments arranged by size:
3181 414 335 248 61 35
BanII G_rGCy'C
Cuts at: 737 949 1193 1288 2318 737
Size: 212 244 95 1030 2693
Fragments arranged by size:
2693 1030 244 212 95
BbeI G_GCGC'C
Cuts at: 898 1708 1956 898
Size: 810 248 3216
Fragments arranged by size:
3216 810 248
BbsI GAAGACnn'nnnn_
Cuts at: 286 581 961 286
Size: 295 380 3599
Fragments arranged by size:
3599 380 295
BciVI GTATCCnnnnn_n'
Cuts at: 2172 2933 4274 2172
Size: 761 1341 2172
Fragments arranged by size:
2172 1341 761
BclI T'GATC_A
Cuts at: 1794 1794
Size: 4274
BglI GCCn_nnn'nGGC
Cuts at: 892 944 892
Size: 52 4222
BglII A'GATC_T
Cuts at: 1789 3826 1789
Size: 2037 2237
BlpI GC'TnA_GC
Cuts at: 736 1386 736
Size: 650 3624
Bme1580I G_kGCm'C
Cuts at: 1899 1992 3048 1899
Size: 93 1056 3125
Fragments arranged by size:
3125 1056 93
BmrI ACTGGGnnnn_n'
Cuts at: 1486 1567 1902 4046 1486
Size: 81 335 2144 1714
Fragments arranged by size:
2144 1714 335 81
BplI GAGnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 2641 2641
Size: 4274
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 388 568 691 1227 388
Size: 180 123 536 3435
Fragments arranged by size:
3435 536 180 123
Bpu10I CC'TnA_GC
Cuts at: 133 133
Size: 4274
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 1450 2329 2821 3119 1450
Size: 879 492 298 2605
Fragments arranged by size:
2605 879 492 298
BsaI GGTCTCn'nnnn_
Cuts at: 508 646 3978 508
Size: 138 3332 804
Fragments arranged by size:
3332 804 138
BsaAI yAC'GTr
Cuts at: 323 1627 323
Size: 1304 2970
BsaBI GATnn'nnATC
Cuts at: 6 1788 3825 6
Size: 1782 2037 455
Fragments arranged by size:
2037 1782 455
BsaHI Gr'CG_yC
Cuts at: 895 1705 1953 2655 4151 895
Size: 810 248 702 1496 1018
Fragments arranged by size:
1496 1018 810 702 248
BsaWI w'CCGG_w
Cuts at: 644 815 1463 1984 2662 2936 3083 3494 644
Size: 171 648 521 678 274 147 411 1424
Fragments arranged by size:
1424 678 648 521 411 274 171 147
BsaXI ACnnnnnCTCCnnnnnnn_nnn'
Cuts at: 964 994 964
Size: 30 4244
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 523 526 784 787 796 523
Size: 3 258 3 9 4001
Fragments arranged by size:
4001 258 9 3 3
BseYI C'CCAG_C
Cuts at: 1699 2457 3034 3530 1699
Size: 758 577 496 2443
Fragments arranged by size:
2443 758 577 496
BsgI GTGCAGnnnnnnnnnnnnnn_nn'
Cuts at: 494 494
Size: 4274
BsiEI CG_ry'CG
Cuts at: 104 730 1149 1816 1862 3070 104
Size: 626 419 667 46 1208 1308
Fragments arranged by size:
1308 1208 667 626 419 46
BsiHKAI G_wGCw'C
Cuts at: 173 1288 2066 2256 3048 173
Size: 1115 778 190 792 1399
Fragments arranged by size:
1399 1115 792 778 190
BsmBI CGTCTCn'nnnn_
Cuts at: 472 472
Size: 4274
Bsp1286I G_dGCh'C
Cuts at: 173 737 949 1193 1288 1899 1992 2066 2256
Size: 564 212 244 95 611 93 74 190
Cuts at: 2256 2318 3048 173
Size: 62 730 1399
Fragments arranged by size:
1399 730 611 564 244 212 190 95
93 74 62
BspEI T'CCGG_A
Cuts at: 644 3494 644
Size: 2850 1424
BspHI T'CATG_A
Cuts at: 3450 3450
Size: 4274
BspMI ACCTGCnnnn'nnnn_
Cuts at: 1324 1538 1840 2221 1324
Size: 214 302 381 3377
Fragments arranged by size:
3377 381 302 214
BsrBI CCG'CTC
Cuts at: 50 2566 2620 4104 4269 50
Size: 2516 54 1484 165 55
Fragments arranged by size:
2516 1484 165 55 54
BsrDI GCAATG_nn'
Cuts at: 2186 2186
Size: 4274
BsrFI r'CCGG_y
Cuts at: 241 450 725 2272 2453 2662 241
Size: 209 275 1547 181 209 1853
Fragments arranged by size:
1853 1547 275 209 209 181
BssHII G'CGCG_C
Cuts at: 190 190
Size: 4274
BssSI C'ACGA_G
Cuts at: 1014 2545 2903 1014
Size: 1531 358 2385
Fragments arranged by size:
2385 1531 358
BstAPI GCAn_nnn'nTGC
Cuts at: 1079 1088 1097 1651 1079
Size: 9 9 554 3702
Fragments arranged by size:
3702 554 9 9
BstBI TT'CG_AA
Cuts at: 2635 2635
Size: 4274
BstEII G'GTnAC_C
Cuts at: 1153 1153
Size: 4274
BstYI r'GATC_y
Cuts at: 1 1299 1766 1789 2124 2370 3382 3468 3480
Size: 1298 467 23 335 246 1012 86 12
Cuts at: 3480 3826 1
Size: 346 449
Fragments arranged by size:
1298 1012 467 449 346 335 246 86
23 12
Bsu36I CC'TnA_GG
Cuts at: 863 863
Size: 4274
BtgI C'CryG_G
Cuts at: 107 288 1181 107
Size: 181 893 3200
Fragments arranged by size:
3200 893 181
BtgZI GCGATGnnnnnnnnnn'nnnn_
Cuts at: 2224 2405 2568 2224
Size: 181 163 3930
Fragments arranged by size:
3930 181 163
BtsI GCAGTG_nn'
Cuts at: 171 1075 1517 171
Size: 904 442 2928
Fragments arranged by size:
2928 904 442
DraI TTT'AAA
Cuts at: 1273 1273
Size: 4274
DrdI GACnn_nn'nnGTC
Cuts at: 907 1980 2838 907
Size: 1073 858 2343
Fragments arranged by size:
2343 1073 858
EaeI y'GGCC_r
Cuts at: 110 727 764 1859 2033 2424 2451 3796 110
Size: 617 37 1095 174 391 27 1345 588
Fragments arranged by size:
1345 1095 617 588 391 174 37 27
EagI C'GGCC_G
Cuts at: 727 1859 727
Size: 1132 3142
EarI CTCTTCn'nnn_
Cuts at: 2297 2507 2297
Size: 210 4064
EciI GGCGGAnnnnnnnnn_nn'
Cuts at: 1201 2482 2792 2938 4084 1201
Size: 1281 310 146 1146 1391
Fragments arranged by size:
1391 1281 1146 310 146
EcoICRI GAG'CTC
Cuts at: 1286 1286
Size: 4274
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 388 442 523 568 691 1227 1255 2099 2531
Size: 54 81 45 123 536 28 844 432
Cuts at: 2531 3278 3546 388
Size: 747 268 1116
Fragments arranged by size:
1116 844 747 536 432 268 123 81
54 45 28
EcoNI CCTnn'n_nnAGG
Cuts at: 142 424 766 142
Size: 282 342 3650
Fragments arranged by size:
3650 342 282
EcoO109I rG'GnC_Cy
Cuts at: 372 386 858 972 1413 372
Size: 14 472 114 441 3233
Fragments arranged by size:
3233 472 441 114 14
EcoRI G'AATT_C
Cuts at: 1278 1278
Size: 4274
EcoRV GAT'ATC
Cuts at: 702 702
Size: 4274
FalI AAGnnnnnCTTnnnnnnnn_nnnnn'
Cuts at: 2293 2293
Size: 4274
FspI TGC'GCA
Cuts at: 2055 2055
Size: 4274
HaeII r_GCGC'y
Cuts at: 898 1708 1956 2978 3957 898
Size: 810 248 1022 979 1215
Fragments arranged by size:
1215 1022 979 810 248
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 347 379 948 964 980 996 1069 1101 2116
Size: 32 569 16 16 16 73 32 1015
Cuts at: 2116 2146 2148 2178 347
Size: 30 2 30 2443
Fragments arranged by size:
2443 1015 569 73 32 32 30 30
16 16 16 2
HincII GTy'rAC
Cuts at: 1313 1313
Size: 4274
KasI G'GCGC_C
Cuts at: 894 1704 1952 894
Size: 810 248 3216
Fragments arranged by size:
3216 810 248
KpnI G_GTAC'C
Cuts at: 1294 1294
Size: 4274
MluI A'CGCG_T
Cuts at: 3707 3707
Size: 4274
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 319 2945 3129 319
Size: 2626 184 1464
Fragments arranged by size:
2626 1464 184
MscI TGG'CCA
Cuts at: 112 2035 112
Size: 1923 2351
MslI CAynn'nnrTG
Cuts at: 271 2263 2390 271
Size: 1992 127 2155
Fragments arranged by size:
2155 1992 127
MspA1I CmG'CkG
Cuts at: 290 335 1064 1125 1385 1699 2059 3072 3317
Size: 45 729 61 260 314 360 1013 245
Cuts at: 3317 290
Size: 1247
Fragments arranged by size:
1247 1013 729 360 314 260 245 61
45
NaeI GCC'GGC
Cuts at: 2455 2455
Size: 4274
NarI GG'CG_CC
Cuts at: 895 1705 1953 895
Size: 810 248 3216
Fragments arranged by size:
3216 810 248
NcoI C'CATG_G
Cuts at: 107 1181 107
Size: 1074 3200
NdeI CA'TA_TG
Cuts at: 627 627
Size: 4274
NgoMIV G'CCGG_C
Cuts at: 2453 2453
Size: 4274
NspI r_CATG'y
Cuts at: 1164 1327 1827 2734 1164
Size: 163 500 907 2704
Fragments arranged by size:
2704 907 500 163
PciI A'CATG_T
Cuts at: 1160 2730 1160
Size: 1570 2704
PfoI T'CCnGG_A
Cuts at: 365 440 365
Size: 75 4199
PmeI GTTT'AAAC
Cuts at: 1273 1273
Size: 4274
PpiI GAACnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 3413 3445 3413
Size: 32 4242
PpuMI rG'GwC_Cy
Cuts at: 372 858 972 372
Size: 486 114 3674
Fragments arranged by size:
3674 486 114
PspXI vC'TCGA_Gb
Cuts at: 457 565 457
Size: 108 4166
PsrI GAACnnnnnnTACnnnnnnn_nnnnn'
Cuts at: 247 279 247
Size: 32 4242
PstI C_TGCA'G
Cuts at: 1093 1321 2006 1093
Size: 228 685 3361
Fragments arranged by size:
3361 685 228
PvuI CG_AT'CG
Cuts at: 104 1149 104
Size: 1045 3229
PvuII CAG'CTG
Cuts at: 1125 1699 2059 1125
Size: 574 360 3340
Fragments arranged by size:
3340 574 360
RsrII CG'GwC_CG
Cuts at: 2469 2469
Size: 4274
SacI G_AGCT'C
Cuts at: 1288 1288
Size: 4274
SacII CC_GC'GG
Cuts at: 291 291
Size: 4274
SalI G'TCGA_C
Cuts at: 1311 1311
Size: 4274
SanDI GG'GwC_CC
Cuts at: 858 858
Size: 4274
SapI GCTCTTCn'nnn_
Cuts at: 2297 2507 2297
Size: 210 4064
SbfI CC_TGCA'GG
Cuts at: 1321 1321
Size: 4274
ScaI AGT'ACT
Cuts at: 3791 3791
Size: 4274
SfcI C'TryA_G
Cuts at: 33 1089 1317 2002 2995 3186 33
Size: 1056 228 685 993 191 1121
Fragments arranged by size:
1121 1056 993 685 228 191
SfoI GGC'GCC
Cuts at: 896 1706 1954 896
Size: 810 248 3216
Fragments arranged by size:
3216 810 248
SgfI GCG_AT'CGC
Cuts at: 104 104
Size: 4274
SmaI CCC'GGG
Cuts at: 308 1296 308
Size: 988 3286
SmlI C'TyrA_G
Cuts at: 457 565 1429 2344 2836 3098 457
Size: 108 864 915 492 262 1633
Fragments arranged by size:
1633 915 864 492 262 108
SpeI A'CTAG_T
Cuts at: 67 67
Size: 4274
SphI G_CATG'C
Cuts at: 1327 1827 1327
Size: 500 3774
StyI C'CwwG_G
Cuts at: 107 138 1181 1408 1762 107
Size: 31 1043 227 354 2619
Fragments arranged by size:
2619 1043 354 227 31
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 692 766 772 1857 1990 2449 2658 692
Size: 74 6 1085 133 459 209 2308
Fragments arranged by size:
2308 1085 459 209 133 74 6
TatI w'GTAC_w
Cuts at: 395 1163 3789 395
Size: 768 2626 880
Fragments arranged by size:
2626 880 768
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 3791 3823 3791
Size: 32 4242
Tth111I GACn'n_nGTC
Cuts at: 2071 2071
Size: 4274
XbaI T'CTAG_A
Cuts at: 1305 1305
Size: 4274
XcmI CCAnnnn_n'nnnnTGG
Cuts at: 1769 1769
Size: 4274
XhoI C'TCGA_G
Cuts at: 457 565 457
Size: 108 4166
XmaI C'CCGG_G
Cuts at: 306 1294 306
Size: 988 3286
Enzymes that do cut:
AccI Acc65I AclI AcuI AflIII AgeI AhdI AlwNI
ApaLI ApoI AseI AsiSI AvaI AvrII BamHI BanI
BanII BbeI BbsI BciVI BclI BglI BglII BlpI
Bme1580I BmrI BplI BpmI Bpu10I BpuEI BsaI BsaAI
BsaBI BsaHI BsaWI BsaXI BseRI BseYI BsgI BsiEI
BsiHKAI BsmBI Bsp1286I BspEI BspHI BspMI BsrBI BsrDI
BsrFI BssHII BssSI BstAPI BstBI BstEII BstYI Bsu36I
BtgI BtgZI BtsI DraI DrdI EaeI EagI EarI
EciI EcoICRI Eco57MI EcoNI EcoO109I EcoRI EcoRV FalI
FspI HaeII Hin4I HincII KasI KpnI MluI MmeI
MscI MslI MspA1I NaeI NarI NcoI NdeI NgoMIV
NspI PciI PfoI PmeI PpiI PpuMI PspXI PsrI
PstI PvuI PvuII RsrII SacI SacII SalI SanDI
SapI SbfI ScaI SfcI SfoI SgfI SmaI SmlI
SpeI SphI StyI TaqII TatI TstI Tth111I XbaI
XcmI XhoI XmaI
Enzymes that do not cut:
AarI AatII AfeI AflII AleI AlfI AloI ApaI
AscI BaeI BbvCI BcgI BfrBI BmgBI BmtI BsiWI
BsmI BsrGI BstXI BstZ17I ClaI CspCI DraIII FseI
FspAI HindIII HpaI MfeI NheI NotI NruI NsiI
PacI PasI PflMI PmlI PshAI PsiI PspOMI SexAI
SfiI SgrAI SnaBI SrfI SspI StuI SwaI XmnI
ZraI