Restriction map for
pF1KB7691
(Circular) (Six-base) MAPSORT of: pF1KB7691.seq Check: 1250 from: 1 to: 4160
pF1KB7691 4160 bp
With 182 enzymes: SgfI *
April 24, 2009 13:08 ..
AccI GT'mk_AC
Cuts at: 1198 1198
Size: 4160
Acc65I G'GTAC_C
Cuts at: 1176 1176
Size: 4160
AclI AA'CG_TT
Cuts at: 4000 4000
Size: 4160
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 348 1985 2417 3164 3432 348
Size: 1637 432 747 268 1076
Fragments arranged by size:
1637 1076 747 432 268
AflIII A'CryG_T
Cuts at: 1512 2616 3593 1512
Size: 1104 977 2079
Fragments arranged by size:
2079 1104 977
AgeI A'CCGG_T
Cuts at: 2548 2548
Size: 4160
AleI CACnn'nnGTG
Cuts at: 655 929 655
Size: 274 3886
AlwNI CAG_nnn'CTG
Cuts at: 1038 1465 3032 1038
Size: 427 1567 2166
Fragments arranged by size:
2166 1567 427
ApaLI G'TGCA_C
Cuts at: 2930 2930
Size: 4160
ApoI r'AATT_y
Cuts at: 463 1164 3513 3769 463
Size: 701 2349 256 854
Fragments arranged by size:
2349 854 701 256
AseI AT'TA_AT
Cuts at: 20 20
Size: 4160
AsiSI GCG_AT'CGC
Cuts at: 104 104
Size: 4160
AvaI C'yCGr_G
Cuts at: 1180 1180
Size: 4160
BamHI G'GATC_C
Cuts at: 1185 1185
Size: 4160
BanI G'GyrC_C
Cuts at: 1176 1590 1838 1873 1176
Size: 414 248 35 3463
Fragments arranged by size:
3463 414 248 35
BanII G_rGCy'C
Cuts at: 593 1069 1174 2204 593
Size: 476 105 1030 2549
Fragments arranged by size:
2549 1030 476 105
BbeI G_GCGC'C
Cuts at: 1594 1842 1594
Size: 248 3912
BbsI GAAGACnn'nnnn_
Cuts at: 652 652
Size: 4160
BciVI GTATCCnnnnn_n'
Cuts at: 2058 2819 4160 2058
Size: 761 1341 2058
Fragments arranged by size:
2058 1341 761
BclI T'GATC_A
Cuts at: 1680 1680
Size: 4160
BglII A'GATC_T
Cuts at: 1675 3712 1675
Size: 2037 2123
BlpI GC'TnA_GC
Cuts at: 1272 1272
Size: 4160
Bme1580I G_kGCm'C
Cuts at: 393 1785 1878 2934 393
Size: 1392 93 1056 1619
Fragments arranged by size:
1619 1392 1056 93
BmgBI CAC'GTC
Cuts at: 627 627
Size: 4160
BmrI ACTGGGnnnn_n'
Cuts at: 943 1372 1453 1788 3932 943
Size: 429 81 335 2144 1171
Fragments arranged by size:
2144 1171 429 335 81
BplI GAGnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 221 612 2527 221
Size: 391 1915 1854
Fragments arranged by size:
1915 1854 391
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 829 829
Size: 4160
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 1336 2215 2707 3005 1336
Size: 879 492 298 2491
Fragments arranged by size:
2491 879 492 298
BsaI GGTCTCn'nnnn_
Cuts at: 490 3864 490
Size: 3374 786
BsaAI yAC'GTr
Cuts at: 1513 1513
Size: 4160
BsaBI GATnn'nnATC
Cuts at: 6 1674 3711 6
Size: 1668 2037 455
Fragments arranged by size:
2037 1668 455
BsaHI Gr'CG_yC
Cuts at: 1591 1839 2541 4037 1591
Size: 248 702 1496 1714
Fragments arranged by size:
1714 1496 702 248
BsaWI w'CCGG_w
Cuts at: 207 1349 1870 2548 2822 2969 3380 207
Size: 1142 521 678 274 147 411 987
Fragments arranged by size:
1142 987 678 521 411 274 147
BsaXI ACnnnnnCTCCnnnnnnn_nnn'
Cuts at: 879 909 879
Size: 30 4130
BseYI C'CCAG_C
Cuts at: 1585 2343 2920 3416 1585
Size: 758 577 496 2329
Fragments arranged by size:
2329 758 577 496
BsgI GTGCAGnnnnnnnnnnnnnn_nn'
Cuts at: 372 670 372
Size: 298 3862
BsiEI CG_ry'CG
Cuts at: 104 1702 1748 2956 104
Size: 1598 46 1208 1308
Fragments arranged by size:
1598 1308 1208 46
BsiHKAI G_wGCw'C
Cuts at: 1174 1952 2142 2934 1174
Size: 778 190 792 2400
Fragments arranged by size:
2400 792 778 190
Bsp1286I G_dGCh'C
Cuts at: 393 593 1069 1174 1785 1878 1952 2142 2204
Size: 200 476 105 611 93 74 190 62
Cuts at: 2204 2934 393
Size: 730 1619
Fragments arranged by size:
1619 730 611 476 200 190 105 93
74 62
BspEI T'CCGG_A
Cuts at: 207 3380 207
Size: 3173 987
BspHI T'CATG_A
Cuts at: 734 830 3336 734
Size: 96 2506 1558
Fragments arranged by size:
2506 1558 96
BspMI ACCTGCnnnn'nnnn_
Cuts at: 709 1210 1424 1726 2107 709
Size: 501 214 302 381 2762
Fragments arranged by size:
2762 501 381 302 214
BsrBI CCG'CTC
Cuts at: 50 395 546 2452 2506 3990 4155 50
Size: 345 151 1906 54 1484 165 55
Fragments arranged by size:
1906 1484 345 165 151 55 54
BsrDI GCAATG_nn'
Cuts at: 2072 2072
Size: 4160
BsrFI r'CCGG_y
Cuts at: 2158 2339 2548 2158
Size: 181 209 3770
Fragments arranged by size:
3770 209 181
BsrGI T'GTAC_A
Cuts at: 274 274
Size: 4160
BssSI C'ACGA_G
Cuts at: 2431 2789 2431
Size: 358 3802
BstAPI GCAn_nnn'nTGC
Cuts at: 693 1537 693
Size: 844 3316
BstBI TT'CG_AA
Cuts at: 2521 2521
Size: 4160
BstYI r'GATC_y
Cuts at: 1 123 1185 1652 1675 2010 2256 3268 3354
Size: 122 1062 467 23 335 246 1012 86
Cuts at: 3354 3366 3712 1
Size: 12 346 449
Fragments arranged by size:
1062 1012 467 449 346 335 246 122
86 23 12
BtgI C'CryG_G
Cuts at: 107 718 899 107
Size: 611 181 3368
Fragments arranged by size:
3368 611 181
BtgZI GCGATGnnnnnnnnnn'nnnn_
Cuts at: 2110 2291 2454 2110
Size: 181 163 3816
Fragments arranged by size:
3816 181 163
BtsI GCAGTG_nn'
Cuts at: 571 1089 1403 571
Size: 518 314 3328
Fragments arranged by size:
3328 518 314
DraI TTT'AAA
Cuts at: 1159 1159
Size: 4160
DraIII CAC_nnn'GTG
Cuts at: 510 510
Size: 4160
DrdI GACnn_nn'nnGTC
Cuts at: 1866 2724 1866
Size: 858 3302
EaeI y'GGCC_r
Cuts at: 568 721 1745 1919 2310 2337 3682 568
Size: 153 1024 174 391 27 1345 1046
Fragments arranged by size:
1345 1046 1024 391 174 153 27
EagI C'GGCC_G
Cuts at: 1745 1745
Size: 4160
EarI CTCTTCn'nnn_
Cuts at: 493 2183 2393 493
Size: 1690 210 2260
Fragments arranged by size:
2260 1690 210
EciI GGCGGAnnnnnnnnn_nn'
Cuts at: 588 2368 2678 2824 3970 588
Size: 1780 310 146 1146 778
Fragments arranged by size:
1780 1146 778 310 146
EcoICRI GAG'CTC
Cuts at: 1172 1172
Size: 4160
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 348 829 1985 2417 3164 3432 348
Size: 481 1156 432 747 268 1076
Fragments arranged by size:
1156 1076 747 481 432 268
EcoO109I rG'GnC_Cy
Cuts at: 1299 1299
Size: 4160
EcoRI G'AATT_C
Cuts at: 1164 1164
Size: 4160
FalI AAGnnnnnCTTnnnnnnnn_nnnnn'
Cuts at: 393 2179 393
Size: 1786 2374
FspI TGC'GCA
Cuts at: 1941 1941
Size: 4160
HaeII r_GCGC'y
Cuts at: 536 1594 1842 2864 3843 536
Size: 1058 248 1022 979 853
Fragments arranged by size:
1058 1022 979 853 248
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 168 200 305 337 510 542 879 911 2002
Size: 32 105 32 173 32 337 32 1091
Cuts at: 2002 2032 2034 2064 168
Size: 30 2 30 2264
Fragments arranged by size:
2264 1091 337 173 105 32 32 32
32 30 30 2
HincII GTy'rAC
Cuts at: 1199 1199
Size: 4160
KasI G'GCGC_C
Cuts at: 1590 1838 1590
Size: 248 3912
KpnI G_GTAC'C
Cuts at: 1180 1180
Size: 4160
MluI A'CGCG_T
Cuts at: 3593 3593
Size: 4160
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 2831 3015 2831
Size: 184 3976
MscI TGG'CCA
Cuts at: 1921 1921
Size: 4160
MslI CAynn'nnrTG
Cuts at: 655 832 929 2149 2276 655
Size: 177 97 1220 127 2539
Fragments arranged by size:
2539 1220 177 127 97
MspA1I CmG'CkG
Cuts at: 425 586 684 720 1271 1585 1945 2958 3203
Size: 161 98 36 551 314 360 1013 245
Cuts at: 3203 425
Size: 1382
Fragments arranged by size:
1382 1013 551 360 314 245 161 98
36
NaeI GCC'GGC
Cuts at: 2341 2341
Size: 4160
NarI GG'CG_CC
Cuts at: 1591 1839 1591
Size: 248 3912
NcoI C'CATG_G
Cuts at: 107 899 107
Size: 792 3368
NgoMIV G'CCGG_C
Cuts at: 2339 2339
Size: 4160
NspI r_CATG'y
Cuts at: 226 233 281 1213 1713 2620 226
Size: 7 48 932 500 907 1766
Fragments arranged by size:
1766 932 907 500 48 7
PasI CC'CwG_GG
Cuts at: 454 454
Size: 4160
PciI A'CATG_T
Cuts at: 2616 2616
Size: 4160
PflMI CCAn_nnn'nTGG
Cuts at: 510 510
Size: 4160
PmeI GTTT'AAAC
Cuts at: 1159 1159
Size: 4160
PpiI GAACnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 3299 3331 3299
Size: 32 4128
PstI C_TGCA'G
Cuts at: 439 1207 1892 439
Size: 768 685 2707
Fragments arranged by size:
2707 768 685
PvuI CG_AT'CG
Cuts at: 104 104
Size: 4160
PvuII CAG'CTG
Cuts at: 684 1585 1945 684
Size: 901 360 2899
Fragments arranged by size:
2899 901 360
RsrII CG'GwC_CG
Cuts at: 2355 2355
Size: 4160
SacI G_AGCT'C
Cuts at: 1174 1174
Size: 4160
SacII CC_GC'GG
Cuts at: 721 721
Size: 4160
SalI G'TCGA_C
Cuts at: 1197 1197
Size: 4160
SapI GCTCTTCn'nnn_
Cuts at: 2183 2393 2183
Size: 210 3950
SbfI CC_TGCA'GG
Cuts at: 1207 1207
Size: 4160
ScaI AGT'ACT
Cuts at: 986 3677 986
Size: 2691 1469
SfcI C'TryA_G
Cuts at: 33 435 1203 1888 2881 3072 33
Size: 402 768 685 993 191 1121
Fragments arranged by size:
1121 993 768 685 402 191
SfoI GGC'GCC
Cuts at: 1592 1840 1592
Size: 248 3912
SgfI GCG_AT'CGC
Cuts at: 104 104
Size: 4160
SmaI CCC'GGG
Cuts at: 1182 1182
Size: 4160
SmlI C'TyrA_G
Cuts at: 1315 2230 2722 2984 1315
Size: 915 492 262 2491
Fragments arranged by size:
2491 915 492 262
SpeI A'CTAG_T
Cuts at: 67 67
Size: 4160
SphI G_CATG'C
Cuts at: 226 1213 1713 226
Size: 987 500 2673
Fragments arranged by size:
2673 987 500
StyI C'CwwG_G
Cuts at: 107 398 899 976 1294 1648 107
Size: 291 501 77 318 354 2619
Fragments arranged by size:
2619 501 354 318 291 77
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 1743 1876 2335 2544 1743
Size: 133 459 209 3359
Fragments arranged by size:
3359 459 209 133
TatI w'GTAC_w
Cuts at: 274 957 984 3675 274
Size: 683 27 2691 759
Fragments arranged by size:
2691 759 683 27
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 3677 3709 3677
Size: 32 4128
Tth111I GACn'n_nGTC
Cuts at: 1957 1957
Size: 4160
XbaI T'CTAG_A
Cuts at: 1191 1191
Size: 4160
XcmI CCAnnnn_n'nnnnTGG
Cuts at: 114 451 1655 114
Size: 337 1204 2619
Fragments arranged by size:
2619 1204 337
XmaI C'CCGG_G
Cuts at: 1180 1180
Size: 4160
XmnI GAAnn'nnTTC
Cuts at: 376 376
Size: 4160
Enzymes that do cut:
AccI Acc65I AclI AcuI AflIII AgeI AleI AlwNI
ApaLI ApoI AseI AsiSI AvaI BamHI BanI BanII
BbeI BbsI BciVI BclI BglII BlpI Bme1580I BmgBI
BmrI BplI BpmI BpuEI BsaI BsaAI BsaBI BsaHI
BsaWI BsaXI BseYI BsgI BsiEI BsiHKAI Bsp1286I BspEI
BspHI BspMI BsrBI BsrDI BsrFI BsrGI BssSI BstAPI
BstBI BstYI BtgI BtgZI BtsI DraI DraIII DrdI
EaeI EagI EarI EciI EcoICRI Eco57MI EcoO109I EcoRI
FalI FspI HaeII Hin4I HincII KasI KpnI MluI
MmeI MscI MslI MspA1I NaeI NarI NcoI NgoMIV
NspI PasI PciI PflMI PmeI PpiI PstI PvuI
PvuII RsrII SacI SacII SalI SapI SbfI ScaI
SfcI SfoI SgfI SmaI SmlI SpeI SphI StyI
TaqII TatI TstI Tth111I XbaI XcmI XmaI XmnI
Enzymes that do not cut:
AarI AatII AfeI AflII AhdI AlfI AloI ApaI
AscI AvrII BaeI BbvCI BcgI BfrBI BglI BmtI
Bpu10I BseRI BsiWI BsmI BsmBI BssHII BstEII BstXI
BstZ17I Bsu36I ClaI CspCI EcoNI EcoRV FseI FspAI
HindIII HpaI MfeI NdeI NheI NotI NruI NsiI
PacI PfoI PmlI PpuMI PshAI PsiI PspOMI PspXI
PsrI SanDI SexAI SfiI SgrAI SnaBI SrfI SspI
StuI SwaI XhoI ZraI