Restriction map for
pF1KB7675
(Circular) (Six-base) MAPSORT of: pF1KB7675.seq Check: 4886 from: 1 to: 4136
pF1KB7675 4136 bp
With 182 enzymes: SgfI *
June 26, 2008 10:49 ..
AccI GT'mk_AC
Cuts at: 1174 1174
Size: 4136
Acc65I G'GTAC_C
Cuts at: 1152 1152
Size: 4136
AclI AA'CG_TT
Cuts at: 3976 3976
Size: 4136
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 1961 2393 3140 3408 1961
Size: 432 747 268 2689
Fragments arranged by size:
2689 747 432 268
AfeI AGC'GCT
Cuts at: 566 566
Size: 4136
AflIII A'CryG_T
Cuts at: 1488 2592 3569 1488
Size: 1104 977 2055
Fragments arranged by size:
2055 1104 977
AgeI A'CCGG_T
Cuts at: 2524 2524
Size: 4136
AleI CACnn'nnGTG
Cuts at: 355 1049 355
Size: 694 3442
AlwNI CAG_nnn'CTG
Cuts at: 1441 3008 1441
Size: 1567 2569
ApaLI G'TGCA_C
Cuts at: 2906 2906
Size: 4136
ApoI r'AATT_y
Cuts at: 1140 3489 3745 1140
Size: 2349 256 1531
Fragments arranged by size:
2349 1531 256
AseI AT'TA_AT
Cuts at: 20 20
Size: 4136
AsiSI GCG_AT'CGC
Cuts at: 104 104
Size: 4136
AvaI C'yCGr_G
Cuts at: 335 637 1156 335
Size: 302 519 3315
Fragments arranged by size:
3315 519 302
BamHI G'GATC_C
Cuts at: 1161 1161
Size: 4136
BanI G'GyrC_C
Cuts at: 624 642 879 1027 1152 1566 1814 1849 624
Size: 18 237 148 125 414 248 35 2911
Fragments arranged by size:
2911 414 248 237 148 125 35 18
BanII G_rGCy'C
Cuts at: 301 472 852 1150 2180 301
Size: 171 380 298 1030 2257
Fragments arranged by size:
2257 1030 380 298 171
BbeI G_GCGC'C
Cuts at: 1570 1818 1570
Size: 248 3888
BcgI CGAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 480 514 480
Size: 34 4102
BciVI GTATCCnnnnn_n'
Cuts at: 2034 2795 4136 2034
Size: 761 1341 2034
Fragments arranged by size:
2034 1341 761
BclI T'GATC_A
Cuts at: 1656 1656
Size: 4136
BglII A'GATC_T
Cuts at: 1651 3688 1651
Size: 2037 2099
BlpI GC'TnA_GC
Cuts at: 1248 1248
Size: 4136
Bme1580I G_kGCm'C
Cuts at: 461 629 1030 1761 1854 2910 461
Size: 168 401 731 93 1056 1687
Fragments arranged by size:
1687 1056 731 401 168 93
BmrI ACTGGGnnnn_n'
Cuts at: 220 1348 1429 1764 3908 220
Size: 1128 81 335 2144 448
Fragments arranged by size:
2144 1128 448 335 81
BplI GAGnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 315 2503 315
Size: 2188 1948
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 874 874
Size: 4136
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 1312 2191 2683 2981 1312
Size: 879 492 298 2467
Fragments arranged by size:
2467 879 492 298
BsaI GGTCTCn'nnnn_
Cuts at: 255 380 895 3840 255
Size: 125 515 2945 551
Fragments arranged by size:
2945 551 515 125
BsaAI yAC'GTr
Cuts at: 1489 1489
Size: 4136
BsaBI GATnn'nnATC
Cuts at: 6 1650 3687 6
Size: 1644 2037 455
Fragments arranged by size:
2037 1644 455
BsaHI Gr'CG_yC
Cuts at: 553 1567 1815 2517 4013 553
Size: 1014 248 702 1496 676
Fragments arranged by size:
1496 1014 702 676 248
BsaWI w'CCGG_w
Cuts at: 368 1325 1846 2524 2798 2945 3356 368
Size: 957 521 678 274 147 411 1148
Fragments arranged by size:
1148 957 678 521 411 274 147
BsaXI ACnnnnnCTCCnnnnnnn_nnn'
Cuts at: 137 167 137
Size: 30 4106
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 158 678 790 158
Size: 520 112 3504
Fragments arranged by size:
3504 520 112
BseYI C'CCAG_C
Cuts at: 138 512 595 894 945 1021 1561 2319 2896
Size: 374 83 299 51 76 540 758 577
Cuts at: 2896 3392 138
Size: 496 882
Fragments arranged by size:
882 758 577 540 496 374 299 83
76 51
BsiEI CG_ry'CG
Cuts at: 104 223 617 1678 1724 2932 104
Size: 119 394 1061 46 1208 1308
Fragments arranged by size:
1308 1208 1061 394 119 46
BsiHKAI G_wGCw'C
Cuts at: 472 1150 1928 2118 2910 472
Size: 678 778 190 792 1698
Fragments arranged by size:
1698 792 778 678 190
BsmI GAATG_Cn'
Cuts at: 482 1088 482
Size: 606 3530
BsmBI CGTCTCn'nnnn_
Cuts at: 286 286
Size: 4136
Bsp1286I G_dGCh'C
Cuts at: 301 461 472 629 852 1030 1150 1761 1854
Size: 160 11 157 223 178 120 611 93
Cuts at: 1854 1928 2118 2180 2910 301
Size: 74 190 62 730 1527
Fragments arranged by size:
1527 730 611 223 190 178 160 157
120 93 74 62 11
BspEI T'CCGG_A
Cuts at: 368 3356 368
Size: 2988 1148
BspHI T'CATG_A
Cuts at: 3312 3312
Size: 4136
BspMI ACCTGCnnnn'nnnn_
Cuts at: 1186 1400 1702 2083 1186
Size: 214 302 381 3239
Fragments arranged by size:
3239 381 302 214
BsrBI CCG'CTC
Cuts at: 50 722 2428 2482 3966 4131 50
Size: 672 1706 54 1484 165 55
Fragments arranged by size:
1706 1484 672 165 55 54
BsrDI GCAATG_nn'
Cuts at: 2048 2048
Size: 4136
BsrFI r'CCGG_y
Cuts at: 2134 2315 2524 2134
Size: 181 209 3746
Fragments arranged by size:
3746 209 181
BssSI C'ACGA_G
Cuts at: 2407 2765 2407
Size: 358 3778
BstAPI GCAn_nnn'nTGC
Cuts at: 1513 1513
Size: 4136
BstBI TT'CG_AA
Cuts at: 2497 2497
Size: 4136
BstYI r'GATC_y
Cuts at: 1 272 410 1161 1628 1651 1986 2232 3244
Size: 271 138 751 467 23 335 246 1012
Cuts at: 3244 3330 3342 3688 1
Size: 86 12 346 449
Fragments arranged by size:
1012 751 467 449 346 335 271 246
138 86 23 12
BtgI C'CryG_G
Cuts at: 107 353 107
Size: 246 3890
BtgZI GCGATGnnnnnnnnnn'nnnn_
Cuts at: 1049 2086 2267 2430 1049
Size: 1037 181 163 2755
Fragments arranged by size:
2755 1037 181 163
BtsI GCAGTG_nn'
Cuts at: 1379 1379
Size: 4136
DraI TTT'AAA
Cuts at: 1135 1135
Size: 4136
DrdI GACnn_nn'nnGTC
Cuts at: 1842 2700 1842
Size: 858 3278
EaeI y'GGCC_r
Cuts at: 614 665 1721 1895 2286 2313 3658 614
Size: 51 1056 174 391 27 1345 1092
Fragments arranged by size:
1345 1092 1056 391 174 51 27
EagI C'GGCC_G
Cuts at: 614 1721 614
Size: 1107 3029
EarI CTCTTCn'nnn_
Cuts at: 2159 2369 2159
Size: 210 3926
EciI GGCGGAnnnnnnnnn_nn'
Cuts at: 445 2344 2654 2800 3946 445
Size: 1899 310 146 1146 635
Fragments arranged by size:
1899 1146 635 310 146
EcoICRI GAG'CTC
Cuts at: 470 1148 470
Size: 678 3458
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 874 1961 2393 3140 3408 874
Size: 1087 432 747 268 1602
Fragments arranged by size:
1602 1087 747 432 268
EcoNI CCTnn'n_nnAGG
Cuts at: 856 856
Size: 4136
EcoO109I rG'GnC_Cy
Cuts at: 1275 1275
Size: 4136
EcoRI G'AATT_C
Cuts at: 1140 1140
Size: 4136
FalI AAGnnnnnCTTnnnnnnnn_nnnnn'
Cuts at: 2155 2155
Size: 4136
FspI TGC'GCA
Cuts at: 490 1917 490
Size: 1427 2709
HaeII r_GCGC'y
Cuts at: 568 1570 1818 2840 3819 568
Size: 1002 248 1022 979 885
Fragments arranged by size:
1022 1002 979 885 248
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 1978 2008 2010 2040 1978
Size: 30 2 30 4074
Fragments arranged by size:
4074 30 30 2
HincII GTy'rAC
Cuts at: 1175 1175
Size: 4136
KasI G'GCGC_C
Cuts at: 1566 1814 1566
Size: 248 3888
KpnI G_GTAC'C
Cuts at: 1156 1156
Size: 4136
MluI A'CGCG_T
Cuts at: 3569 3569
Size: 4136
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 243 818 2807 2991 243
Size: 575 1989 184 1388
Fragments arranged by size:
1989 1388 575 184
MscI TGG'CCA
Cuts at: 667 1897 667
Size: 1230 2906
MslI CAynn'nnrTG
Cuts at: 355 1049 2125 2252 355
Size: 694 1076 127 2239
Fragments arranged by size:
2239 1076 694 127
MspA1I CmG'CkG
Cuts at: 138 168 898 1247 1561 1921 2934 3179 138
Size: 30 730 349 314 360 1013 245 1095
Fragments arranged by size:
1095 1013 730 360 349 314 245 30
NaeI GCC'GGC
Cuts at: 2317 2317
Size: 4136
NarI GG'CG_CC
Cuts at: 1567 1815 1567
Size: 248 3888
NcoI C'CATG_G
Cuts at: 107 107
Size: 4136
NgoMIV G'CCGG_C
Cuts at: 2315 2315
Size: 4136
NotI GC'GGCC_GC
Cuts at: 614 614
Size: 4136
NspI r_CATG'y
Cuts at: 826 1021 1189 1689 2596 826
Size: 195 168 500 907 2366
Fragments arranged by size:
2366 907 500 195 168
PciI A'CATG_T
Cuts at: 2592 2592
Size: 4136
PfoI T'CCnGG_A
Cuts at: 413 413
Size: 4136
PmeI GTTT'AAAC
Cuts at: 1135 1135
Size: 4136
PpiI GAACnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 3275 3307 3275
Size: 32 4104
PstI C_TGCA'G
Cuts at: 1183 1868 1183
Size: 685 3451
PvuI CG_AT'CG
Cuts at: 104 104
Size: 4136
PvuII CAG'CTG
Cuts at: 138 898 1561 1921 138
Size: 760 663 360 2353
Fragments arranged by size:
2353 760 663 360
RsrII CG'GwC_CG
Cuts at: 2331 2331
Size: 4136
SacI G_AGCT'C
Cuts at: 472 1150 472
Size: 678 3458
SalI G'TCGA_C
Cuts at: 1173 1173
Size: 4136
SapI GCTCTTCn'nnn_
Cuts at: 2159 2369 2159
Size: 210 3926
SbfI CC_TGCA'GG
Cuts at: 1183 1183
Size: 4136
ScaI AGT'ACT
Cuts at: 3653 3653
Size: 4136
SfcI C'TryA_G
Cuts at: 33 972 1179 1864 2857 3048 33
Size: 939 207 685 993 191 1121
Fragments arranged by size:
1121 993 939 685 207 191
SfoI GGC'GCC
Cuts at: 1568 1816 1568
Size: 248 3888
SgfI GCG_AT'CGC
Cuts at: 104 104
Size: 4136
SmaI CCC'GGG
Cuts at: 337 639 1158 337
Size: 302 519 3315
Fragments arranged by size:
3315 519 302
SmlI C'TyrA_G
Cuts at: 1291 2206 2698 2960 1291
Size: 915 492 262 2467
Fragments arranged by size:
2467 915 492 262
SpeI A'CTAG_T
Cuts at: 67 67
Size: 4136
SphI G_CATG'C
Cuts at: 826 1189 1689 826
Size: 363 500 3273
Fragments arranged by size:
3273 500 363
StyI C'CwwG_G
Cuts at: 107 620 767 1270 1624 107
Size: 513 147 503 354 2619
Fragments arranged by size:
2619 513 503 354 147
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 998 1719 1852 2311 2520 998
Size: 721 133 459 209 2614
Fragments arranged by size:
2614 721 459 209 133
TatI w'GTAC_w
Cuts at: 449 3651 449
Size: 3202 934
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 137 169 424 456 3653 3685 137
Size: 32 255 32 3197 32 588
Fragments arranged by size:
3197 588 255 32 32 32
Tth111I GACn'n_nGTC
Cuts at: 1933 1933
Size: 4136
XbaI T'CTAG_A
Cuts at: 1167 1167
Size: 4136
XcmI CCAnnnn_n'nnnnTGG
Cuts at: 1631 1631
Size: 4136
XmaI C'CCGG_G
Cuts at: 335 637 1156 335
Size: 302 519 3315
Fragments arranged by size:
3315 519 302
Enzymes that do cut:
AccI Acc65I AclI AcuI AfeI AflIII AgeI AleI
AlwNI ApaLI ApoI AseI AsiSI AvaI BamHI BanI
BanII BbeI BcgI BciVI BclI BglII BlpI Bme1580I
BmrI BplI BpmI BpuEI BsaI BsaAI BsaBI BsaHI
BsaWI BsaXI BseRI BseYI BsiEI BsiHKAI BsmI BsmBI
Bsp1286I BspEI BspHI BspMI BsrBI BsrDI BsrFI BssSI
BstAPI BstBI BstYI BtgI BtgZI BtsI DraI DrdI
EaeI EagI EarI EciI EcoICRI Eco57MI EcoNI EcoO109I
EcoRI FalI FspI HaeII Hin4I HincII KasI KpnI
MluI MmeI MscI MslI MspA1I NaeI NarI NcoI
NgoMIV NotI NspI PciI PfoI PmeI PpiI PstI
PvuI PvuII RsrII SacI SalI SapI SbfI ScaI
SfcI SfoI SgfI SmaI SmlI SpeI SphI StyI
TaqII TatI TstI Tth111I XbaI XcmI XmaI
Enzymes that do not cut:
AarI AatII AflII AhdI AlfI AloI ApaI AscI
AvrII BaeI BbsI BbvCI BfrBI BglI BmgBI BmtI
Bpu10I BsgI BsiWI BsrGI BssHII BstEII BstXI BstZ17I
Bsu36I ClaI CspCI DraIII EcoRV FseI FspAI HindIII
HpaI MfeI NdeI NheI NruI NsiI PacI PasI
PflMI PmlI PpuMI PshAI PsiI PspOMI PspXI PsrI
SacII SanDI SexAI SfiI SgrAI SnaBI SrfI SspI
StuI SwaI XhoI XmnI ZraI