Restriction map for
pF1KB7669
(Circular) (Six-base) MAPSORT of: pF1KB7669.seq Check: 9719 from: 1 to: 4127
pF1KB7669 4127 bp
With 182 enzymes: SgfI *
June 26, 2008 10:49 ..
AarI CACCTGCnnnn'nnnn_
Cuts at: 1036 1036
Size: 4127
AccI GT'mk_AC
Cuts at: 389 1165 389
Size: 776 3351
Acc65I G'GTAC_C
Cuts at: 1143 1143
Size: 4127
AclI AA'CG_TT
Cuts at: 3967 3967
Size: 4127
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 846 1952 2384 3131 3399 846
Size: 1106 432 747 268 1574
Fragments arranged by size:
1574 1106 747 432 268
AflIII A'CryG_T
Cuts at: 1479 2583 3560 1479
Size: 1104 977 2046
Fragments arranged by size:
2046 1104 977
AgeI A'CCGG_T
Cuts at: 2515 2515
Size: 4127
AlfI GCAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 636 636
Size: 4127
AloI GAACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 1090 1122 1090
Size: 32 4095
AlwNI CAG_nnn'CTG
Cuts at: 366 1432 2999 366
Size: 1066 1567 1494
Fragments arranged by size:
1567 1494 1066
ApaI G_GGCC'C
Cuts at: 797 808 797
Size: 11 4116
ApaLI G'TGCA_C
Cuts at: 2897 2897
Size: 4127
ApoI r'AATT_y
Cuts at: 1131 3480 3736 1131
Size: 2349 256 1522
Fragments arranged by size:
2349 1522 256
AseI AT'TA_AT
Cuts at: 20 20
Size: 4127
AsiSI GCG_AT'CGC
Cuts at: 104 104
Size: 4127
AvaI C'yCGr_G
Cuts at: 247 521 542 796 1147 247
Size: 274 21 254 351 3227
Fragments arranged by size:
3227 351 274 254 21
AvrII C'CTAG_G
Cuts at: 532 532
Size: 4127
BamHI G'GATC_C
Cuts at: 1152 1152
Size: 4127
BanI G'GyrC_C
Cuts at: 1054 1143 1557 1805 1840 1054
Size: 89 414 248 35 3341
Fragments arranged by size:
3341 414 248 89 35
BanII G_rGCy'C
Cuts at: 528 719 797 808 838 1141 2171 528
Size: 191 78 11 30 303 1030 2484
Fragments arranged by size:
2484 1030 303 191 78 30 11
BbeI G_GCGC'C
Cuts at: 1058 1561 1809 1058
Size: 503 248 3376
Fragments arranged by size:
3376 503 248
BbsI GAAGACnn'nnnn_
Cuts at: 179 179
Size: 4127
BciVI GTATCCnnnnn_n'
Cuts at: 2025 2786 4127 2025
Size: 761 1341 2025
Fragments arranged by size:
2025 1341 761
BclI T'GATC_A
Cuts at: 1647 1647
Size: 4127
BglII A'GATC_T
Cuts at: 1642 3679 1642
Size: 2037 2090
BlpI GC'TnA_GC
Cuts at: 837 1239 837
Size: 402 3725
Bme1580I G_kGCm'C
Cuts at: 275 797 808 1752 1845 2901 275
Size: 522 11 944 93 1056 1501
Fragments arranged by size:
1501 1056 944 522 93 11
BmrI ACTGGGnnnn_n'
Cuts at: 342 1339 1420 1755 3899 342
Size: 997 81 335 2144 570
Fragments arranged by size:
2144 997 570 335 81
BplI GAGnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 2494 2494
Size: 4127
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 292 640 718 760 292
Size: 348 78 42 3659
Fragments arranged by size:
3659 348 78 42
Bpu10I CC'TnA_GC
Cuts at: 848 848
Size: 4127
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 1303 2182 2674 2972 1303
Size: 879 492 298 2458
Fragments arranged by size:
2458 879 492 298
BsaI GGTCTCn'nnnn_
Cuts at: 250 531 1044 3831 250
Size: 281 513 2787 546
Fragments arranged by size:
2787 546 513 281
BsaAI yAC'GTr
Cuts at: 1480 1480
Size: 4127
BsaBI GATnn'nnATC
Cuts at: 6 1641 3678 6
Size: 1635 2037 455
Fragments arranged by size:
2037 1635 455
BsaHI Gr'CG_yC
Cuts at: 1055 1558 1806 2508 4004 1055
Size: 503 248 702 1496 1178
Fragments arranged by size:
1496 1178 702 503 248
BsaWI w'CCGG_w
Cuts at: 1316 1837 2515 2789 2936 3347 1316
Size: 521 678 274 147 411 2096
Fragments arranged by size:
2096 678 521 411 274 147
BsaXI ACnnnnnCTCCnnnnnnn_nnn'
Cuts at: 121 127 151 157 1090 1120 121
Size: 6 24 6 933 30 3128
Fragments arranged by size:
3128 933 30 24 6 6
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 147 562 586 1102 147
Size: 415 24 516 3172
Fragments arranged by size:
3172 516 415 24
BseYI C'CCAG_C
Cuts at: 374 516 1552 2310 2887 3383 374
Size: 142 1036 758 577 496 1118
Fragments arranged by size:
1118 1036 758 577 496 142
BsgI GTGCAGnnnnnnnnnnnnnn_nn'
Cuts at: 171 171
Size: 4127
BsiEI CG_ry'CG
Cuts at: 104 386 651 1669 1715 2923 104
Size: 282 265 1018 46 1208 1308
Fragments arranged by size:
1308 1208 1018 282 265 46
BsiHKAI G_wGCw'C
Cuts at: 719 1141 1919 2109 2901 719
Size: 422 778 190 792 1945
Fragments arranged by size:
1945 792 778 422 190
BsmBI CGTCTCn'nnnn_
Cuts at: 544 544
Size: 4127
Bsp1286I G_dGCh'C
Cuts at: 275 528 719 797 808 838 1141 1752 1845
Size: 253 191 78 11 30 303 611 93
Cuts at: 1845 1919 2109 2171 2901 275
Size: 74 190 62 730 1501
Fragments arranged by size:
1501 730 611 303 253 191 190 93
78 74 62 30 11
BspEI T'CCGG_A
Cuts at: 3347 3347
Size: 4127
BspHI T'CATG_A
Cuts at: 3303 3303
Size: 4127
BspMI ACCTGCnnnn'nnnn_
Cuts at: 1036 1177 1391 1693 2074 1036
Size: 141 214 302 381 3089
Fragments arranged by size:
3089 381 302 214 141
BsrBI CCG'CTC
Cuts at: 50 2419 2473 3957 4122 50
Size: 2369 54 1484 165 55
Fragments arranged by size:
2369 1484 165 55 54
BsrDI GCAATG_nn'
Cuts at: 2039 2039
Size: 4127
BsrFI r'CCGG_y
Cuts at: 241 629 843 1051 2125 2306 2515 241
Size: 388 214 208 1074 181 209 1853
Fragments arranged by size:
1853 1074 388 214 209 208 181
BssSI C'ACGA_G
Cuts at: 2398 2756 2398
Size: 358 3769
BstAPI GCAn_nnn'nTGC
Cuts at: 1504 1504
Size: 4127
BstBI TT'CG_AA
Cuts at: 2488 2488
Size: 4127
BstEII G'GTnAC_C
Cuts at: 279 279
Size: 4127
BstYI r'GATC_y
Cuts at: 1 776 1152 1619 1642 1977 2223 3235 3321
Size: 775 376 467 23 335 246 1012 86
Cuts at: 3321 3333 3679 1
Size: 12 346 449
Fragments arranged by size:
1012 775 467 449 376 346 335 246
86 23 12
BtgI C'CryG_G
Cuts at: 275 341 275
Size: 66 4061
BtgZI GCGATGnnnnnnnnnn'nnnn_
Cuts at: 2077 2258 2421 2077
Size: 181 163 3783
Fragments arranged by size:
3783 181 163
BtsI GCAGTG_nn'
Cuts at: 453 471 1080 1370 453
Size: 18 609 290 3210
Fragments arranged by size:
3210 609 290 18
DraI TTT'AAA
Cuts at: 1126 1126
Size: 4127
DraIII CAC_nnn'GTG
Cuts at: 958 958
Size: 4127
DrdI GACnn_nn'nnGTC
Cuts at: 1833 2691 1833
Size: 858 3269
EaeI y'GGCC_r
Cuts at: 1712 1886 2277 2304 3649 1712
Size: 174 391 27 1345 2190
Fragments arranged by size:
2190 1345 391 174 27
EagI C'GGCC_G
Cuts at: 1712 1712
Size: 4127
EarI CTCTTCn'nnn_
Cuts at: 342 563 782 2150 2360 342
Size: 221 219 1368 210 2109
Fragments arranged by size:
2109 1368 221 219 210
EciI GGCGGAnnnnnnnnn_nn'
Cuts at: 467 648 676 829 2335 2645 2791 3937 467
Size: 181 28 153 1506 310 146 1146 657
Fragments arranged by size:
1506 1146 657 310 181 153 146 28
EcoICRI GAG'CTC
Cuts at: 717 1139 717
Size: 422 3705
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 292 640 718 760 846 1952 2384 3131 3399
Size: 348 78 42 86 1106 432 747 268
Cuts at: 3399 292
Size: 1020
Fragments arranged by size:
1106 1020 747 432 348 268 86 78
42
EcoO109I rG'GnC_Cy
Cuts at: 303 793 1266 303
Size: 490 473 3164
Fragments arranged by size:
3164 490 473
EcoRI G'AATT_C
Cuts at: 1131 1131
Size: 4127
FalI AAGnnnnnCTTnnnnnnnn_nnnnn'
Cuts at: 2146 2146
Size: 4127
FspI TGC'GCA
Cuts at: 1908 1908
Size: 4127
HaeII r_GCGC'y
Cuts at: 1058 1561 1809 2831 3810 1058
Size: 503 248 1022 979 1375
Fragments arranged by size:
1375 1022 979 503 248
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 121 127 153 159 685 717 927 959 1969
Size: 6 26 6 526 32 210 32 1010
Cuts at: 1969 1999 2001 2031 121
Size: 30 2 30 2217
Fragments arranged by size:
2217 1010 526 210 32 32 30 30
26 6 6 2
HincII GTy'rAC
Cuts at: 390 996 1166 390
Size: 606 170 3351
Fragments arranged by size:
3351 606 170
HpaI GTT'AAC
Cuts at: 996 996
Size: 4127
KasI G'GCGC_C
Cuts at: 1054 1557 1805 1054
Size: 503 248 3376
Fragments arranged by size:
3376 503 248
KpnI G_GTAC'C
Cuts at: 1147 1147
Size: 4127
MluI A'CGCG_T
Cuts at: 3560 3560
Size: 4127
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 656 683 2798 2982 656
Size: 27 2115 184 1801
Fragments arranged by size:
2115 1801 184 27
MscI TGG'CCA
Cuts at: 1888 1888
Size: 4127
MslI CAynn'nnrTG
Cuts at: 2116 2243 2116
Size: 127 4000
MspA1I CmG'CkG
Cuts at: 485 810 866 1070 1238 1552 1912 2925 3170
Size: 325 56 204 168 314 360 1013 245
Cuts at: 3170 485
Size: 1442
Fragments arranged by size:
1442 1013 360 325 314 245 204 168
56
NaeI GCC'GGC
Cuts at: 1053 2308 1053
Size: 1255 2872
NarI GG'CG_CC
Cuts at: 1055 1558 1806 1055
Size: 503 248 3376
Fragments arranged by size:
3376 503 248
NcoI C'CATG_G
Cuts at: 341 341
Size: 4127
NgoMIV G'CCGG_C
Cuts at: 1051 2306 1051
Size: 1255 2872
NspI r_CATG'y
Cuts at: 405 1180 1680 2587 405
Size: 775 500 907 1945
Fragments arranged by size:
1945 907 775 500
PasI CC'CwG_GG
Cuts at: 354 354
Size: 4127
PciI A'CATG_T
Cuts at: 2583 2583
Size: 4127
PmeI GTTT'AAAC
Cuts at: 1126 1126
Size: 4127
PpiI GAACnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 1090 1122 3266 3298 1090
Size: 32 2144 32 1919
Fragments arranged by size:
2144 1919 32 32
PpuMI rG'GwC_Cy
Cuts at: 303 303
Size: 4127
PspOMI G'GGCC_C
Cuts at: 793 804 793
Size: 11 4116
PstI C_TGCA'G
Cuts at: 1174 1859 1174
Size: 685 3442
PvuI CG_AT'CG
Cuts at: 104 104
Size: 4127
PvuII CAG'CTG
Cuts at: 866 1552 1912 866
Size: 686 360 3081
Fragments arranged by size:
3081 686 360
RsrII CG'GwC_CG
Cuts at: 2322 2322
Size: 4127
SacI G_AGCT'C
Cuts at: 719 1141 719
Size: 422 3705
SalI G'TCGA_C
Cuts at: 388 1164 388
Size: 776 3351
SapI GCTCTTCn'nnn_
Cuts at: 2150 2360 2150
Size: 210 3917
SbfI CC_TGCA'GG
Cuts at: 1174 1174
Size: 4127
ScaI AGT'ACT
Cuts at: 3644 3644
Size: 4127
SfcI C'TryA_G
Cuts at: 33 441 1170 1855 2848 3039 33
Size: 408 729 685 993 191 1121
Fragments arranged by size:
1121 993 729 685 408 191
SfoI GGC'GCC
Cuts at: 1056 1559 1807 1056
Size: 503 248 3376
Fragments arranged by size:
3376 503 248
SgfI GCG_AT'CGC
Cuts at: 104 104
Size: 4127
SgrAI Cr'CCGG_yG
Cuts at: 1051 1051
Size: 4127
SmaI CCC'GGG
Cuts at: 798 1149 798
Size: 351 3776
SmlI C'TyrA_G
Cuts at: 1282 2197 2689 2951 1282
Size: 915 492 262 2458
Fragments arranged by size:
2458 915 492 262
SpeI A'CTAG_T
Cuts at: 67 67
Size: 4127
SphI G_CATG'C
Cuts at: 1180 1680 1180
Size: 500 3627
SrfI GCCC'GGGC
Cuts at: 798 798
Size: 4127
StyI C'CwwG_G
Cuts at: 341 532 1261 1615 341
Size: 191 729 354 2853
Fragments arranged by size:
2853 729 354 191
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 228 661 665 1710 1843 2302 2511 228
Size: 433 4 1045 133 459 209 1844
Fragments arranged by size:
1844 1045 459 433 209 133 4
TatI w'GTAC_w
Cuts at: 3642 3642
Size: 4127
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 295 327 3644 3676 295
Size: 32 3317 32 746
Fragments arranged by size:
3317 746 32 32
Tth111I GACn'n_nGTC
Cuts at: 1924 1924
Size: 4127
XbaI T'CTAG_A
Cuts at: 1158 1158
Size: 4127
XcmI CCAnnnn_n'nnnnTGG
Cuts at: 1622 1622
Size: 4127
XmaI C'CCGG_G
Cuts at: 796 1147 796
Size: 351 3776
Enzymes that do cut:
AarI AccI Acc65I AclI AcuI AflIII AgeI AlfI
AloI AlwNI ApaI ApaLI ApoI AseI AsiSI AvaI
AvrII BamHI BanI BanII BbeI BbsI BciVI BclI
BglII BlpI Bme1580I BmrI BplI BpmI Bpu10I BpuEI
BsaI BsaAI BsaBI BsaHI BsaWI BsaXI BseRI BseYI
BsgI BsiEI BsiHKAI BsmBI Bsp1286I BspEI BspHI BspMI
BsrBI BsrDI BsrFI BssSI BstAPI BstBI BstEII BstYI
BtgI BtgZI BtsI DraI DraIII DrdI EaeI EagI
EarI EciI EcoICRI Eco57MI EcoO109I EcoRI FalI FspI
HaeII Hin4I HincII HpaI KasI KpnI MluI MmeI
MscI MslI MspA1I NaeI NarI NcoI NgoMIV NspI
PasI PciI PmeI PpiI PpuMI PspOMI PstI PvuI
PvuII RsrII SacI SalI SapI SbfI ScaI SfcI
SfoI SgfI SgrAI SmaI SmlI SpeI SphI SrfI
StyI TaqII TatI TstI Tth111I XbaI XcmI XmaI
Enzymes that do not cut:
AatII AfeI AflII AhdI AleI AscI BaeI BbvCI
BcgI BfrBI BglI BmgBI BmtI BsiWI BsmI BsrGI
BssHII BstXI BstZ17I Bsu36I ClaI CspCI EcoNI EcoRV
FseI FspAI HindIII MfeI NdeI NheI NotI NruI
NsiI PacI PflMI PfoI PmlI PshAI PsiI PspXI
PsrI SacII SanDI SexAI SfiI SnaBI SspI StuI
SwaI XhoI XmnI ZraI