Restriction map for
pF1KB7662
(Circular) (Six-base) MAPSORT of: pF1KB7662.seq Check: 7463 from: 1 to: 4301
pF1KB7662 4301 bp
With 182 enzymes: SgfI *
October 7, 2009 14:24 ..
AarI CACCTGCnnnn'nnnn_
Cuts at: 609 609
Size: 4301
AccI GT'mk_AC
Cuts at: 387 1339 387
Size: 952 3349
Acc65I G'GTAC_C
Cuts at: 1317 1317
Size: 4301
AclI AA'CG_TT
Cuts at: 4141 4141
Size: 4301
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 212 2126 2558 3305 3573 212
Size: 1914 432 747 268 940
Fragments arranged by size:
1914 940 747 432 268
AflIII A'CryG_T
Cuts at: 1653 2757 3734 1653
Size: 1104 977 2220
Fragments arranged by size:
2220 1104 977
AgeI A'CCGG_T
Cuts at: 2689 2689
Size: 4301
AlwNI CAG_nnn'CTG
Cuts at: 424 1606 3173 424
Size: 1182 1567 1552
Fragments arranged by size:
1567 1552 1182
ApaLI G'TGCA_C
Cuts at: 3071 3071
Size: 4301
ApoI r'AATT_y
Cuts at: 630 763 805 1023 1305 3654 3910 630
Size: 133 42 218 282 2349 256 1021
Fragments arranged by size:
2349 1021 282 256 218 133 42
AseI AT'TA_AT
Cuts at: 20 20
Size: 4301
AsiSI GCG_AT'CGC
Cuts at: 104 104
Size: 4301
AvaI C'yCGr_G
Cuts at: 614 1321 614
Size: 707 3594
BamHI G'GATC_C
Cuts at: 113 1326 113
Size: 1213 3088
BanI G'GyrC_C
Cuts at: 457 1317 1731 1979 2014 457
Size: 860 414 248 35 2744
Fragments arranged by size:
2744 860 414 248 35
BanII G_rGCy'C
Cuts at: 239 623 1315 2345 239
Size: 384 692 1030 2195
Fragments arranged by size:
2195 1030 692 384
BbeI G_GCGC'C
Cuts at: 1735 1983 1735
Size: 248 4053
BbsI GAAGACnn'nnnn_
Cuts at: 602 602
Size: 4301
BbvCI CC'TCA_GC
Cuts at: 142 142
Size: 4301
BciVI GTATCCnnnnn_n'
Cuts at: 2199 2960 4301 2199
Size: 761 1341 2199
Fragments arranged by size:
2199 1341 761
BclI T'GATC_A
Cuts at: 1821 1821
Size: 4301
BglII A'GATC_T
Cuts at: 1816 3853 1816
Size: 2037 2264
BlpI GC'TnA_GC
Cuts at: 1413 1413
Size: 4301
Bme1580I G_kGCm'C
Cuts at: 717 1926 2019 3075 717
Size: 1209 93 1056 1943
Fragments arranged by size:
1943 1209 1056 93
BmrI ACTGGGnnnn_n'
Cuts at: 112 1513 1594 1929 4073 112
Size: 1401 81 335 2144 340
Fragments arranged by size:
2144 1401 340 335 81
BplI GAGnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 2668 2668
Size: 4301
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 427 427
Size: 4301
Bpu10I CC'TnA_GC
Cuts at: 142 142
Size: 4301
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 1193 1477 2356 2848 3146 1193
Size: 284 879 492 298 2348
Fragments arranged by size:
2348 879 492 298 284
BsaI GGTCTCn'nnnn_
Cuts at: 346 4005 346
Size: 3659 642
BsaAI yAC'GTr
Cuts at: 330 1654 330
Size: 1324 2977
BsaBI GATnn'nnATC
Cuts at: 6 1815 3852 6
Size: 1809 2037 455
Fragments arranged by size:
2037 1809 455
BsaHI Gr'CG_yC
Cuts at: 298 1732 1980 2682 4178 298
Size: 1434 248 702 1496 421
Fragments arranged by size:
1496 1434 702 421 248
BsaWI w'CCGG_w
Cuts at: 270 1490 2011 2689 2963 3110 3521 270
Size: 1220 521 678 274 147 411 1050
Fragments arranged by size:
1220 1050 678 521 411 274 147
BsaXI ACnnnnnCTCCnnnnnnn_nnn'
Cuts at: 895 925 895
Size: 30 4271
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 237 237
Size: 4301
BseYI C'CCAG_C
Cuts at: 1726 2484 3061 3557 1726
Size: 758 577 496 2470
Fragments arranged by size:
2470 758 577 496
BsgI GTGCAGnnnnnnnnnnnnnn_nn'
Cuts at: 880 880
Size: 4301
BsiEI CG_ry'CG
Cuts at: 104 339 1843 1889 3097 104
Size: 235 1504 46 1208 1308
Fragments arranged by size:
1504 1308 1208 235 46
BsiHKAI G_wGCw'C
Cuts at: 1315 2093 2283 3075 1315
Size: 778 190 792 2541
Fragments arranged by size:
2541 792 778 190
Bsp1286I G_dGCh'C
Cuts at: 239 623 717 1315 1926 2019 2093 2283 2345
Size: 384 94 598 611 93 74 190 62
Cuts at: 2345 3075 239
Size: 730 1465
Fragments arranged by size:
1465 730 611 598 384 190 94 93
74 62
BspEI T'CCGG_A
Cuts at: 3521 3521
Size: 4301
BspHI T'CATG_A
Cuts at: 3477 3477
Size: 4301
BspMI ACCTGCnnnn'nnnn_
Cuts at: 609 1351 1565 1867 2248 609
Size: 742 214 302 381 2662
Fragments arranged by size:
2662 742 381 302 214
BsrBI CCG'CTC
Cuts at: 50 2593 2647 4131 4296 50
Size: 2543 54 1484 165 55
Fragments arranged by size:
2543 1484 165 55 54
BsrDI GCAATG_nn'
Cuts at: 2213 2213
Size: 4301
BsrFI r'CCGG_y
Cuts at: 216 2299 2480 2689 216
Size: 2083 181 209 1828
Fragments arranged by size:
2083 1828 209 181
BssSI C'ACGA_G
Cuts at: 2572 2930 2572
Size: 358 3943
BstAPI GCAn_nnn'nTGC
Cuts at: 1678 1678
Size: 4301
BstBI TT'CG_AA
Cuts at: 2662 2662
Size: 4301
BstYI r'GATC_y
Cuts at: 1 113 570 825 1326 1793 1816 2151 2397
Size: 112 457 255 501 467 23 335 246
Cuts at: 2397 3409 3495 3507 3853 1
Size: 1012 86 12 346 449
Fragments arranged by size:
1012 501 467 457 449 346 335 255
246 112 86 23 12
Bsu36I CC'TnA_GG
Cuts at: 513 513
Size: 4301
BtgZI GCGATGnnnnnnnnnn'nnnn_
Cuts at: 2251 2432 2595 2251
Size: 181 163 3957
Fragments arranged by size:
3957 181 163
BtsI GCAGTG_nn'
Cuts at: 1544 1544
Size: 4301
DraI TTT'AAA
Cuts at: 564 674 1300 564
Size: 110 626 3565
Fragments arranged by size:
3565 626 110
DrdI GACnn_nn'nnGTC
Cuts at: 2007 2865 2007
Size: 858 3443
EaeI y'GGCC_r
Cuts at: 1886 2060 2451 2478 3823 1886
Size: 174 391 27 1345 2364
Fragments arranged by size:
2364 1345 391 174 27
EagI C'GGCC_G
Cuts at: 1886 1886
Size: 4301
EarI CTCTTCn'nnn_
Cuts at: 2324 2534 2324
Size: 210 4091
EciI GGCGGAnnnnnnnnn_nn'
Cuts at: 141 275 2509 2819 2965 4111 141
Size: 134 2234 310 146 1146 331
Fragments arranged by size:
2234 1146 331 310 146 134
EcoICRI GAG'CTC
Cuts at: 1313 1313
Size: 4301
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 212 427 2126 2558 3305 3573 212
Size: 215 1699 432 747 268 940
Fragments arranged by size:
1699 940 747 432 268 215
EcoNI CCTnn'n_nnAGG
Cuts at: 436 436
Size: 4301
EcoO109I rG'GnC_Cy
Cuts at: 421 1440 421
Size: 1019 3282
EcoRI G'AATT_C
Cuts at: 805 1305 805
Size: 500 3801
FalI AAGnnnnnCTTnnnnnnnn_nnnnn'
Cuts at: 327 2320 327
Size: 1993 2308
FspI TGC'GCA
Cuts at: 2082 2082
Size: 4301
HaeII r_GCGC'y
Cuts at: 167 1106 1735 1983 3005 3984 167
Size: 939 629 248 1022 979 484
Fragments arranged by size:
1022 979 939 629 484 248
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 895 927 1002 1034 2143 2173 2175 2205 895
Size: 32 75 32 1109 30 2 30 2991
Fragments arranged by size:
2991 1109 75 32 32 30 30 2
HincII GTy'rAC
Cuts at: 1197 1340 1197
Size: 143 4158
HpaI GTT'AAC
Cuts at: 1197 1197
Size: 4301
KasI G'GCGC_C
Cuts at: 1731 1979 1731
Size: 248 4053
KpnI G_GTAC'C
Cuts at: 1321 1321
Size: 4301
MluI A'CGCG_T
Cuts at: 3734 3734
Size: 4301
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 663 2972 3156 663
Size: 2309 184 1808
Fragments arranged by size:
2309 1808 184
MscI TGG'CCA
Cuts at: 2062 2062
Size: 4301
MslI CAynn'nnrTG
Cuts at: 2290 2417 2290
Size: 127 4174
MspA1I CmG'CkG
Cuts at: 258 945 1412 1726 2086 3099 3344 258
Size: 687 467 314 360 1013 245 1215
Fragments arranged by size:
1215 1013 687 467 360 314 245
NaeI GCC'GGC
Cuts at: 218 2482 218
Size: 2264 2037
NarI GG'CG_CC
Cuts at: 1732 1980 1732
Size: 248 4053
NdeI CA'TA_TG
Cuts at: 732 732
Size: 4301
NgoMIV G'CCGG_C
Cuts at: 216 2480 216
Size: 2264 2037
NspI r_CATG'y
Cuts at: 1203 1354 1854 2761 1203
Size: 151 500 907 2743
Fragments arranged by size:
2743 907 500 151
PciI A'CATG_T
Cuts at: 2757 2757
Size: 4301
PflMI CCAn_nnn'nTGG
Cuts at: 424 424
Size: 4301
PfoI T'CCnGG_A
Cuts at: 423 828 423
Size: 405 3896
PmeI GTTT'AAAC
Cuts at: 1300 1300
Size: 4301
PpiI GAACnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 3440 3472 3440
Size: 32 4269
PpuMI rG'GwC_Cy
Cuts at: 421 421
Size: 4301
PsiI TTA'TAA
Cuts at: 1262 1262
Size: 4301
PstI C_TGCA'G
Cuts at: 1348 2033 1348
Size: 685 3616
PvuI CG_AT'CG
Cuts at: 104 339 104
Size: 235 4066
PvuII CAG'CTG
Cuts at: 258 1726 2086 258
Size: 1468 360 2473
Fragments arranged by size:
2473 1468 360
RsrII CG'GwC_CG
Cuts at: 2496 2496
Size: 4301
SacI G_AGCT'C
Cuts at: 1315 1315
Size: 4301
SalI G'TCGA_C
Cuts at: 1338 1338
Size: 4301
SapI GCTCTTCn'nnn_
Cuts at: 2324 2534 2324
Size: 210 4091
SbfI CC_TGCA'GG
Cuts at: 1348 1348
Size: 4301
ScaI AGT'ACT
Cuts at: 3818 3818
Size: 4301
SfcI C'TryA_G
Cuts at: 33 1235 1344 2029 3022 3213 33
Size: 1202 109 685 993 191 1121
Fragments arranged by size:
1202 1121 993 685 191 109
SfoI GGC'GCC
Cuts at: 1733 1981 1733
Size: 248 4053
SgfI GCG_AT'CGC
Cuts at: 104 104
Size: 4301
SmaI CCC'GGG
Cuts at: 1323 1323
Size: 4301
SmlI C'TyrA_G
Cuts at: 614 1208 1456 2371 2863 3125 614
Size: 594 248 915 492 262 1790
Fragments arranged by size:
1790 915 594 492 262 248
SnaBI TAC'GTA
Cuts at: 330 330
Size: 4301
SpeI A'CTAG_T
Cuts at: 67 67
Size: 4301
SphI G_CATG'C
Cuts at: 1354 1854 1354
Size: 500 3801
StyI C'CwwG_G
Cuts at: 155 289 652 907 1435 1789 155
Size: 134 363 255 528 354 2667
Fragments arranged by size:
2667 528 363 354 255 134
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 1884 2017 2476 2685 1884
Size: 133 459 209 3500
Fragments arranged by size:
3500 459 209 133
TatI w'GTAC_w
Cuts at: 748 3816 748
Size: 3068 1233
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 216 248 3818 3850 216
Size: 32 3570 32 667
Fragments arranged by size:
3570 667 32 32
Tth111I GACn'n_nGTC
Cuts at: 2098 2098
Size: 4301
XbaI T'CTAG_A
Cuts at: 1332 1332
Size: 4301
XcmI CCAnnnn_n'nnnnTGG
Cuts at: 1796 1796
Size: 4301
XhoI C'TCGA_G
Cuts at: 614 614
Size: 4301
XmaI C'CCGG_G
Cuts at: 1321 1321
Size: 4301
XmnI GAAnn'nnTTC
Cuts at: 647 647
Size: 4301
Enzymes that do cut:
AarI AccI Acc65I AclI AcuI AflIII AgeI AlwNI
ApaLI ApoI AseI AsiSI AvaI BamHI BanI BanII
BbeI BbsI BbvCI BciVI BclI BglII BlpI Bme1580I
BmrI BplI BpmI Bpu10I BpuEI BsaI BsaAI BsaBI
BsaHI BsaWI BsaXI BseRI BseYI BsgI BsiEI BsiHKAI
Bsp1286I BspEI BspHI BspMI BsrBI BsrDI BsrFI BssSI
BstAPI BstBI BstYI Bsu36I BtgZI BtsI DraI DrdI
EaeI EagI EarI EciI EcoICRI Eco57MI EcoNI EcoO109I
EcoRI FalI FspI HaeII Hin4I HincII HpaI KasI
KpnI MluI MmeI MscI MslI MspA1I NaeI NarI
NdeI NgoMIV NspI PciI PflMI PfoI PmeI PpiI
PpuMI PsiI PstI PvuI PvuII RsrII SacI SalI
SapI SbfI ScaI SfcI SfoI SgfI SmaI SmlI
SnaBI SpeI SphI StyI TaqII TatI TstI Tth111I
XbaI XcmI XhoI XmaI XmnI
Enzymes that do not cut:
AatII AfeI AflII AhdI AleI AlfI AloI ApaI
AscI AvrII BaeI BcgI BfrBI BglI BmgBI BmtI
BsiWI BsmI BsmBI BsrGI BssHII BstEII BstXI BstZ17I
BtgI ClaI CspCI DraIII EcoRV FseI FspAI HindIII
MfeI NcoI NheI NotI NruI NsiI PacI PasI
PmlI PshAI PspOMI PspXI PsrI SacII SanDI SexAI
SfiI SgrAI SrfI SspI StuI SwaI ZraI