Restriction map for
pF1KB7658
(Circular) (Six-base) MAPSORT of: pF1KB7658.seq Check: 712 from: 1 to: 4295
pF1KB7658 4295 bp
With 182 enzymes: SgfI *
June 20, 2008 10:27 ..
AccI GT'mk_AC
Cuts at: 809 1333 809
Size: 524 3771
Acc65I G'GTAC_C
Cuts at: 1311 1311
Size: 4295
AclI AA'CG_TT
Cuts at: 4135 4135
Size: 4295
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 331 611 632 2120 2552 3299 3567 331
Size: 280 21 1488 432 747 268 1059
Fragments arranged by size:
1488 1059 747 432 280 268 21
AflIII A'CryG_T
Cuts at: 1647 2751 3728 1647
Size: 1104 977 2214
Fragments arranged by size:
2214 1104 977
AgeI A'CCGG_T
Cuts at: 2683 2683
Size: 4295
AlwNI CAG_nnn'CTG
Cuts at: 1600 3167 1600
Size: 1567 2728
ApaI G_GGCC'C
Cuts at: 224 224
Size: 4295
ApaLI G'TGCA_C
Cuts at: 3065 3065
Size: 4295
ApoI r'AATT_y
Cuts at: 1004 1062 1299 3648 3904 1004
Size: 58 237 2349 256 1395
Fragments arranged by size:
2349 1395 256 237 58
AseI AT'TA_AT
Cuts at: 20 20
Size: 4295
AsiSI GCG_AT'CGC
Cuts at: 104 104
Size: 4295
AvaI C'yCGr_G
Cuts at: 1315 1315
Size: 4295
BaeI ACnnnnGTAyCnnnnnnn_nnnnn'
Cuts at: 849 882 849
Size: 33 4262
BamHI G'GATC_C
Cuts at: 507 1320 507
Size: 813 3482
BanI G'GyrC_C
Cuts at: 1311 1725 1973 2008 1311
Size: 414 248 35 3598
Fragments arranged by size:
3598 414 248 35
BanII G_rGCy'C
Cuts at: 224 340 1309 2339 224
Size: 116 969 1030 2180
Fragments arranged by size:
2180 1030 969 116
BbeI G_GCGC'C
Cuts at: 1729 1977 1729
Size: 248 4047
BbsI GAAGACnn'nnnn_
Cuts at: 638 638
Size: 4295
BciVI GTATCCnnnnn_n'
Cuts at: 2193 2954 4295 2193
Size: 761 1341 2193
Fragments arranged by size:
2193 1341 761
BclI T'GATC_A
Cuts at: 1815 1815
Size: 4295
BglI GCCn_nnn'nGGC
Cuts at: 112 112
Size: 4295
BglII A'GATC_T
Cuts at: 1810 3847 1810
Size: 2037 2258
BlpI GC'TnA_GC
Cuts at: 1407 1407
Size: 4295
Bme1580I G_kGCm'C
Cuts at: 224 1920 2013 3069 224
Size: 1696 93 1056 1450
Fragments arranged by size:
1696 1450 1056 93
BmgBI CAC'GTC
Cuts at: 527 527
Size: 4295
BmrI ACTGGGnnnn_n'
Cuts at: 287 1507 1588 1923 4067 287
Size: 1220 81 335 2144 515
Fragments arranged by size:
2144 1220 515 335 81
BplI GAGnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 813 2662 813
Size: 1849 2446
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 268 950 268
Size: 682 3613
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 324 1471 2350 2842 3140 324
Size: 1147 879 492 298 1479
Fragments arranged by size:
1479 1147 879 492 298
BsaI GGTCTCn'nnnn_
Cuts at: 304 684 1182 3999 304
Size: 380 498 2817 600
Fragments arranged by size:
2817 600 498 380
BsaAI yAC'GTr
Cuts at: 1648 1648
Size: 4295
BsaBI GATnn'nnATC
Cuts at: 6 1809 3846 6
Size: 1803 2037 455
Fragments arranged by size:
2037 1803 455
BsaHI Gr'CG_yC
Cuts at: 1726 1974 2676 4172 1726
Size: 248 702 1496 1849
Fragments arranged by size:
1849 1496 702 248
BsaWI w'CCGG_w
Cuts at: 1484 2005 2683 2957 3104 3515 1484
Size: 521 678 274 147 411 2264
Fragments arranged by size:
2264 678 521 411 274 147
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 460 773 1165 460
Size: 313 392 3590
Fragments arranged by size:
3590 392 313
BseYI C'CCAG_C
Cuts at: 186 1720 2478 3055 3551 186
Size: 1534 758 577 496 930
Fragments arranged by size:
1534 930 758 577 496
BsiEI CG_ry'CG
Cuts at: 104 1837 1883 3091 104
Size: 1733 46 1208 1308
Fragments arranged by size:
1733 1308 1208 46
BsiHKAI G_wGCw'C
Cuts at: 254 340 1309 2087 2277 3069 254
Size: 86 969 778 190 792 1480
Fragments arranged by size:
1480 969 792 778 190 86
Bsp1286I G_dGCh'C
Cuts at: 224 254 340 1309 1920 2013 2087 2277 2339
Size: 30 86 969 611 93 74 190 62
Cuts at: 2339 3069 224
Size: 730 1450
Fragments arranged by size:
1450 969 730 611 190 93 86 74
62 30
BspEI T'CCGG_A
Cuts at: 3515 3515
Size: 4295
BspHI T'CATG_A
Cuts at: 1080 1176 3471 1080
Size: 96 2295 1904
Fragments arranged by size:
2295 1904 96
BspMI ACCTGCnnnn'nnnn_
Cuts at: 1345 1559 1861 2242 1345
Size: 214 302 381 3398
Fragments arranged by size:
3398 381 302 214
BsrBI CCG'CTC
Cuts at: 50 2587 2641 4125 4290 50
Size: 2537 54 1484 165 55
Fragments arranged by size:
2537 1484 165 55 54
BsrDI GCAATG_nn'
Cuts at: 2207 2207
Size: 4295
BsrFI r'CCGG_y
Cuts at: 2293 2474 2683 2293
Size: 181 209 3905
Fragments arranged by size:
3905 209 181
BssSI C'ACGA_G
Cuts at: 2566 2924 2566
Size: 358 3937
BstAPI GCAn_nnn'nTGC
Cuts at: 1672 1672
Size: 4295
BstBI TT'CG_AA
Cuts at: 2656 2656
Size: 4295
BstYI r'GATC_y
Cuts at: 1 507 600 1320 1787 1810 2145 2391 3403
Size: 506 93 720 467 23 335 246 1012
Cuts at: 3403 3489 3501 3847 1
Size: 86 12 346 449
Fragments arranged by size:
1012 720 506 467 449 346 335 246
93 86 23 12
Bsu36I CC'TnA_GG
Cuts at: 443 443
Size: 4295
BtgZI GCGATGnnnnnnnnnn'nnnn_
Cuts at: 2245 2426 2589 2245
Size: 181 163 3951
Fragments arranged by size:
3951 181 163
BtsI GCAGTG_nn'
Cuts at: 480 555 1538 480
Size: 75 983 3237
Fragments arranged by size:
3237 983 75
CspCI CAAnnnnnGTGGnnnnnnnnnn_nn'
Cuts at: 1191 1226 1191
Size: 35 4260
DraI TTT'AAA
Cuts at: 1294 1294
Size: 4295
DraIII CAC_nnn'GTG
Cuts at: 874 874
Size: 4295
DrdI GACnn_nn'nnGTC
Cuts at: 550 2001 2859 550
Size: 1451 858 1986
Fragments arranged by size:
1986 1451 858
EaeI y'GGCC_r
Cuts at: 1880 2054 2445 2472 3817 1880
Size: 174 391 27 1345 2358
Fragments arranged by size:
2358 1345 391 174 27
EagI C'GGCC_G
Cuts at: 1880 1880
Size: 4295
EarI CTCTTCn'nnn_
Cuts at: 2318 2528 2318
Size: 210 4085
EciI GGCGGAnnnnnnnnn_nn'
Cuts at: 161 2503 2813 2959 4105 161
Size: 2342 310 146 1146 351
Fragments arranged by size:
2342 1146 351 310 146
EcoICRI GAG'CTC
Cuts at: 338 1307 338
Size: 969 3326
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 268 331 611 632 950 2120 2552 3299 3567
Size: 63 280 21 318 1170 432 747 268
Cuts at: 3567 268
Size: 996
Fragments arranged by size:
1170 996 747 432 318 280 268 63
21
EcoO109I rG'GnC_Cy
Cuts at: 920 1434 920
Size: 514 3781
EcoRI G'AATT_C
Cuts at: 1004 1062 1299 1004
Size: 58 237 4000
Fragments arranged by size:
4000 237 58
FalI AAGnnnnnCTTnnnnnnnn_nnnnn'
Cuts at: 171 217 330 2314 171
Size: 46 113 1984 2152
Fragments arranged by size:
2152 1984 113 46
FspI TGC'GCA
Cuts at: 2076 2076
Size: 4295
HaeII r_GCGC'y
Cuts at: 1729 1977 2999 3978 1729
Size: 248 1022 979 2046
Fragments arranged by size:
2046 1022 979 248
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 322 354 403 405 435 437 492 524 2137
Size: 32 49 2 30 2 55 32 1613
Cuts at: 2137 2167 2169 2199 322
Size: 30 2 30 2418
Fragments arranged by size:
2418 1613 55 49 32 32 30 30
30 2 2 2
HincII GTy'rAC
Cuts at: 1334 1334
Size: 4295
HindIII A'AGCT_T
Cuts at: 379 966 379
Size: 587 3708
KasI G'GCGC_C
Cuts at: 1725 1973 1725
Size: 248 4047
KpnI G_GTAC'C
Cuts at: 1315 1315
Size: 4295
MluI A'CGCG_T
Cuts at: 3728 3728
Size: 4295
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 2966 3150 2966
Size: 184 4111
MscI TGG'CCA
Cuts at: 2056 2056
Size: 4295
MslI CAynn'nnrTG
Cuts at: 2284 2411 2284
Size: 127 4168
MspA1I CmG'CkG
Cuts at: 1406 1720 2080 3093 3338 1406
Size: 314 360 1013 245 2363
Fragments arranged by size:
2363 1013 360 314 245
NaeI GCC'GGC
Cuts at: 2476 2476
Size: 4295
NarI GG'CG_CC
Cuts at: 1726 1974 1726
Size: 248 4047
NdeI CA'TA_TG
Cuts at: 1194 1194
Size: 4295
NgoMIV G'CCGG_C
Cuts at: 2474 2474
Size: 4295
NspI r_CATG'y
Cuts at: 1348 1848 2755 1348
Size: 500 907 2888
Fragments arranged by size:
2888 907 500
PciI A'CATG_T
Cuts at: 2751 2751
Size: 4295
PflMI CCAn_nnn'nTGG
Cuts at: 874 1126 874
Size: 252 4043
PmeI GTTT'AAAC
Cuts at: 1294 1294
Size: 4295
PpiI GAACnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 3434 3466 3434
Size: 32 4263
PpuMI rG'GwC_Cy
Cuts at: 920 920
Size: 4295
PspOMI G'GGCC_C
Cuts at: 220 220
Size: 4295
PstI C_TGCA'G
Cuts at: 1172 1342 2027 1172
Size: 170 685 3440
Fragments arranged by size:
3440 685 170
PvuI CG_AT'CG
Cuts at: 104 104
Size: 4295
PvuII CAG'CTG
Cuts at: 1720 2080 1720
Size: 360 3935
RsrII CG'GwC_CG
Cuts at: 2490 2490
Size: 4295
SacI G_AGCT'C
Cuts at: 340 1309 340
Size: 969 3326
SalI G'TCGA_C
Cuts at: 1332 1332
Size: 4295
SapI GCTCTTCn'nnn_
Cuts at: 2318 2528 2318
Size: 210 4085
SbfI CC_TGCA'GG
Cuts at: 1342 1342
Size: 4295
ScaI AGT'ACT
Cuts at: 3812 3812
Size: 4295
SfcI C'TryA_G
Cuts at: 33 617 1168 1338 2023 3016 3207 33
Size: 584 551 170 685 993 191 1121
Fragments arranged by size:
1121 993 685 584 551 191 170
SfoI GGC'GCC
Cuts at: 1727 1975 1727
Size: 248 4047
SgfI GCG_AT'CGC
Cuts at: 104 104
Size: 4295
SmaI CCC'GGG
Cuts at: 1317 1317
Size: 4295
SmlI C'TyrA_G
Cuts at: 339 1450 2365 2857 3119 339
Size: 1111 915 492 262 1515
Fragments arranged by size:
1515 1111 915 492 262
SpeI A'CTAG_T
Cuts at: 67 67
Size: 4295
SphI G_CATG'C
Cuts at: 1348 1848 1348
Size: 500 3795
StyI C'CwwG_G
Cuts at: 314 371 674 1429 1783 314
Size: 57 303 755 354 2826
Fragments arranged by size:
2826 755 354 303 57
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 1878 2011 2470 2679 1878
Size: 133 459 209 3494
Fragments arranged by size:
3494 459 209 133
TatI w'GTAC_w
Cuts at: 3810 3810
Size: 4295
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 3812 3844 3812
Size: 32 4263
Tth111I GACn'n_nGTC
Cuts at: 417 2092 417
Size: 1675 2620
XbaI T'CTAG_A
Cuts at: 786 1326 786
Size: 540 3755
XcmI CCAnnnn_n'nnnnTGG
Cuts at: 279 1790 279
Size: 1511 2784
XmaI C'CCGG_G
Cuts at: 1315 1315
Size: 4295
XmnI GAAnn'nnTTC
Cuts at: 967 1135 967
Size: 168 4127
Enzymes that do cut:
AccI Acc65I AclI AcuI AflIII AgeI AlwNI ApaI
ApaLI ApoI AseI AsiSI AvaI BaeI BamHI BanI
BanII BbeI BbsI BciVI BclI BglI BglII BlpI
Bme1580I BmgBI BmrI BplI BpmI BpuEI BsaI BsaAI
BsaBI BsaHI BsaWI BseRI BseYI BsiEI BsiHKAI Bsp1286I
BspEI BspHI BspMI BsrBI BsrDI BsrFI BssSI BstAPI
BstBI BstYI Bsu36I BtgZI BtsI CspCI DraI DraIII
DrdI EaeI EagI EarI EciI EcoICRI Eco57MI EcoO109I
EcoRI FalI FspI HaeII Hin4I HincII HindIII KasI
KpnI MluI MmeI MscI MslI MspA1I NaeI NarI
NdeI NgoMIV NspI PciI PflMI PmeI PpiI PpuMI
PspOMI PstI PvuI PvuII RsrII SacI SalI SapI
SbfI ScaI SfcI SfoI SgfI SmaI SmlI SpeI
SphI StyI TaqII TatI TstI Tth111I XbaI XcmI
XmaI XmnI
Enzymes that do not cut:
AarI AatII AfeI AflII AhdI AleI AlfI AloI
AscI AvrII BbvCI BcgI BfrBI BmtI Bpu10I BsaXI
BsgI BsiWI BsmI BsmBI BsrGI BssHII BstEII BstXI
BstZ17I BtgI ClaI EcoNI EcoRV FseI FspAI HpaI
MfeI NcoI NheI NotI NruI NsiI PacI PasI
PfoI PmlI PshAI PsiI PspXI PsrI SacII SanDI
SexAI SfiI SgrAI SnaBI SrfI SspI StuI SwaI
XhoI ZraI