Restriction map for
pF1KB7649
(Circular) (Six-base) MAPSORT of: pF1KB7649.seq Check: 1375 from: 1 to: 4100
pF1KB7649 4100 bp
With 182 enzymes: SgfI *
June 20, 2008 10:27 ..
AccI GT'mk_AC
Cuts at: 1138 1138
Size: 4100
Acc65I G'GTAC_C
Cuts at: 1116 1116
Size: 4100
AclI AA'CG_TT
Cuts at: 3940 3940
Size: 4100
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 691 710 803 1925 2357 3104 3372 691
Size: 19 93 1122 432 747 268 1419
Fragments arranged by size:
1419 1122 747 432 268 93 19
AflIII A'CryG_T
Cuts at: 1452 2556 3533 1452
Size: 1104 977 2019
Fragments arranged by size:
2019 1104 977
AgeI A'CCGG_T
Cuts at: 2488 2488
Size: 4100
AleI CACnn'nnGTG
Cuts at: 844 844
Size: 4100
AlwNI CAG_nnn'CTG
Cuts at: 1405 2972 1405
Size: 1567 2533
ApaI G_GGCC'C
Cuts at: 139 139
Size: 4100
ApaLI G'TGCA_C
Cuts at: 963 2870 963
Size: 1907 2193
ApoI r'AATT_y
Cuts at: 420 1104 3453 3709 420
Size: 684 2349 256 811
Fragments arranged by size:
2349 811 684 256
AseI AT'TA_AT
Cuts at: 20 20
Size: 4100
AsiSI GCG_AT'CGC
Cuts at: 104 104
Size: 4100
AvaI C'yCGr_G
Cuts at: 138 200 1040 1120 138
Size: 62 840 80 3118
Fragments arranged by size:
3118 840 80 62
BamHI G'GATC_C
Cuts at: 795 1125 795
Size: 330 3770
BanI G'GyrC_C
Cuts at: 976 1116 1530 1778 1813 976
Size: 140 414 248 35 3263
Fragments arranged by size:
3263 414 248 140 35
BanII G_rGCy'C
Cuts at: 139 734 1114 2144 139
Size: 595 380 1030 2095
Fragments arranged by size:
2095 1030 595 380
BbeI G_GCGC'C
Cuts at: 1534 1782 1534
Size: 248 3852
BcgI CGAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 159 193 159
Size: 34 4066
BciVI GTATCCnnnnn_n'
Cuts at: 1998 2759 4100 1998
Size: 761 1341 1998
Fragments arranged by size:
1998 1341 761
BclI T'GATC_A
Cuts at: 1620 1620
Size: 4100
BglI GCCn_nnn'nGGC
Cuts at: 164 164
Size: 4100
BglII A'GATC_T
Cuts at: 355 1615 3652 355
Size: 1260 2037 803
Fragments arranged by size:
2037 1260 803
BlpI GC'TnA_GC
Cuts at: 653 1212 653
Size: 559 3541
Bme1580I G_kGCm'C
Cuts at: 139 967 1725 1818 2874 139
Size: 828 758 93 1056 1365
Fragments arranged by size:
1365 1056 828 758 93
BmgBI CAC'GTC
Cuts at: 749 749
Size: 4100
BmrI ACTGGGnnnn_n'
Cuts at: 911 1312 1393 1728 3872 911
Size: 401 81 335 2144 1139
Fragments arranged by size:
2144 1139 401 335 81
BplI GAGnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 2467 2467
Size: 4100
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 115 489 616 796 115
Size: 374 127 180 3419
Fragments arranged by size:
3419 374 180 127
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 1276 2155 2647 2945 1276
Size: 879 492 298 2431
Fragments arranged by size:
2431 879 492 298
BsaI GGTCTCn'nnnn_
Cuts at: 380 3804 380
Size: 3424 676
BsaAI yAC'GTr
Cuts at: 1453 1453
Size: 4100
BsaBI GATnn'nnATC
Cuts at: 6 1614 3651 6
Size: 1608 2037 455
Fragments arranged by size:
2037 1608 455
BsaHI Gr'CG_yC
Cuts at: 1531 1779 2481 3977 1531
Size: 248 702 1496 1654
Fragments arranged by size:
1654 1496 702 248
BsaWI w'CCGG_w
Cuts at: 1289 1810 2488 2762 2909 3320 1289
Size: 521 678 274 147 411 2069
Fragments arranged by size:
2069 678 521 411 274 147
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 368 368
Size: 4100
BseYI C'CCAG_C
Cuts at: 116 546 1525 2283 2860 3356 116
Size: 430 979 758 577 496 860
Fragments arranged by size:
979 860 758 577 496 430
BsgI GTGCAGnnnnnnnnnnnnnn_nn'
Cuts at: 309 433 309
Size: 124 3976
BsiEI CG_ry'CG
Cuts at: 104 1642 1688 2896 104
Size: 1538 46 1208 1308
Fragments arranged by size:
1538 1308 1208 46
BsiHKAI G_wGCw'C
Cuts at: 967 1114 1892 2082 2874 967
Size: 147 778 190 792 2193
Fragments arranged by size:
2193 792 778 190 147
Bsp1286I G_dGCh'C
Cuts at: 139 734 967 1114 1725 1818 1892 2082 2144
Size: 595 233 147 611 93 74 190 62
Cuts at: 2144 2874 139
Size: 730 1365
Fragments arranged by size:
1365 730 611 595 233 190 147 93
74 62
BspEI T'CCGG_A
Cuts at: 3320 3320
Size: 4100
BspHI T'CATG_A
Cuts at: 3276 3276
Size: 4100
BspMI ACCTGCnnnn'nnnn_
Cuts at: 1150 1364 1666 2047 1150
Size: 214 302 381 3203
Fragments arranged by size:
3203 381 302 214
BsrBI CCG'CTC
Cuts at: 50 2392 2446 3930 4095 50
Size: 2342 54 1484 165 55
Fragments arranged by size:
2342 1484 165 55 54
BsrDI GCAATG_nn'
Cuts at: 2012 2012
Size: 4100
BsrFI r'CCGG_y
Cuts at: 2098 2279 2488 2098
Size: 181 209 3710
Fragments arranged by size:
3710 209 181
BssSI C'ACGA_G
Cuts at: 160 2371 2729 160
Size: 2211 358 1531
Fragments arranged by size:
2211 1531 358
BstAPI GCAn_nnn'nTGC
Cuts at: 1477 1477
Size: 4100
BstBI TT'CG_AA
Cuts at: 2461 2461
Size: 4100
BstXI CCAn_nnnn'nTGG
Cuts at: 621 621
Size: 4100
BstYI r'GATC_y
Cuts at: 1 355 795 1125 1592 1615 1950 2196 3208
Size: 354 440 330 467 23 335 246 1012
Cuts at: 3208 3294 3306 3652 1
Size: 86 12 346 449
Fragments arranged by size:
1012 467 449 440 354 346 335 330
246 86 23 12
Bsu36I CC'TnA_GG
Cuts at: 810 1061 810
Size: 251 3849
BtgI C'CryG_G
Cuts at: 145 145
Size: 4100
BtgZI GCGATGnnnnnnnnnn'nnnn_
Cuts at: 2050 2231 2394 2050
Size: 181 163 3756
Fragments arranged by size:
3756 181 163
BtsI GCAGTG_nn'
Cuts at: 1343 1343
Size: 4100
DraI TTT'AAA
Cuts at: 1099 1099
Size: 4100
DrdI GACnn_nn'nnGTC
Cuts at: 1806 2664 1806
Size: 858 3242
EaeI y'GGCC_r
Cuts at: 1685 1859 2250 2277 3622 1685
Size: 174 391 27 1345 2163
Fragments arranged by size:
2163 1345 391 174 27
EagI C'GGCC_G
Cuts at: 1685 1685
Size: 4100
EarI CTCTTCn'nnn_
Cuts at: 567 2123 2333 567
Size: 1556 210 2334
Fragments arranged by size:
2334 1556 210
EciI GGCGGAnnnnnnnnn_nn'
Cuts at: 145 2308 2618 2764 3910 145
Size: 2163 310 146 1146 335
Fragments arranged by size:
2163 1146 335 310 146
EcoICRI GAG'CTC
Cuts at: 1112 1112
Size: 4100
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 115 489 616 691 710 796 803 1925 2357
Size: 374 127 75 19 86 7 1122 432
Cuts at: 2357 3104 3372 115
Size: 747 268 843
Fragments arranged by size:
1122 843 747 432 374 268 127 86
75 19 7
EcoO109I rG'GnC_Cy
Cuts at: 135 444 1239 135
Size: 309 795 2996
Fragments arranged by size:
2996 795 309
EcoRI G'AATT_C
Cuts at: 1104 1104
Size: 4100
FalI AAGnnnnnCTTnnnnnnnn_nnnnn'
Cuts at: 2119 2119
Size: 4100
FspI TGC'GCA
Cuts at: 1881 1881
Size: 4100
HaeII r_GCGC'y
Cuts at: 464 1534 1782 2804 3783 464
Size: 1070 248 1022 979 781
Fragments arranged by size:
1070 1022 979 781 248
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 239 271 670 702 1942 1972 1974 2004 239
Size: 32 399 32 1240 30 2 30 2335
Fragments arranged by size:
2335 1240 399 32 32 30 30 2
HincII GTy'rAC
Cuts at: 1139 1139
Size: 4100
KasI G'GCGC_C
Cuts at: 1530 1778 1530
Size: 248 3852
KpnI G_GTAC'C
Cuts at: 1120 1120
Size: 4100
MluI A'CGCG_T
Cuts at: 3533 3533
Size: 4100
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 602 2771 2955 602
Size: 2169 184 1747
Fragments arranged by size:
2169 1747 184
MscI TGG'CCA
Cuts at: 1861 1861
Size: 4100
MslI CAynn'nnrTG
Cuts at: 844 2089 2216 844
Size: 1245 127 2728
Fragments arranged by size:
2728 1245 127
MspA1I CmG'CkG
Cuts at: 550 617 1211 1525 1885 2898 3143 550
Size: 67 594 314 360 1013 245 1507
Fragments arranged by size:
1507 1013 594 360 314 245 67
NaeI GCC'GGC
Cuts at: 2281 2281
Size: 4100
NarI GG'CG_CC
Cuts at: 1531 1779 1531
Size: 248 3852
NgoMIV G'CCGG_C
Cuts at: 2279 2279
Size: 4100
NspI r_CATG'y
Cuts at: 1039 1153 1653 2560 1039
Size: 114 500 907 2579
Fragments arranged by size:
2579 907 500 114
PciI A'CATG_T
Cuts at: 2556 2556
Size: 4100
PfoI T'CCnGG_A
Cuts at: 210 773 210
Size: 563 3537
PmeI GTTT'AAAC
Cuts at: 1099 1099
Size: 4100
PpiI GAACnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 3239 3271 3239
Size: 32 4068
PpuMI rG'GwC_Cy
Cuts at: 444 444
Size: 4100
PspOMI G'GGCC_C
Cuts at: 135 135
Size: 4100
PstI C_TGCA'G
Cuts at: 1147 1832 1147
Size: 685 3415
PvuI CG_AT'CG
Cuts at: 104 104
Size: 4100
PvuII CAG'CTG
Cuts at: 550 1525 1885 550
Size: 975 360 2765
Fragments arranged by size:
2765 975 360
RsrII CG'GwC_CG
Cuts at: 2295 2295
Size: 4100
SacI G_AGCT'C
Cuts at: 1114 1114
Size: 4100
SalI G'TCGA_C
Cuts at: 1137 1137
Size: 4100
SapI GCTCTTCn'nnn_
Cuts at: 2123 2333 2123
Size: 210 3890
SbfI CC_TGCA'GG
Cuts at: 1147 1147
Size: 4100
ScaI AGT'ACT
Cuts at: 3617 3617
Size: 4100
SfcI C'TryA_G
Cuts at: 33 347 1143 1828 2821 3012 33
Size: 314 796 685 993 191 1121
Fragments arranged by size:
1121 993 796 685 314 191
SfoI GGC'GCC
Cuts at: 1532 1780 1532
Size: 248 3852
SgfI GCG_AT'CGC
Cuts at: 104 104
Size: 4100
SmaI CCC'GGG
Cuts at: 140 202 1042 1122 140
Size: 62 840 80 3118
Fragments arranged by size:
3118 840 80 62
SmlI C'TyrA_G
Cuts at: 1255 2170 2662 2924 1255
Size: 915 492 262 2431
Fragments arranged by size:
2431 915 492 262
SpeI A'CTAG_T
Cuts at: 67 67
Size: 4100
SphI G_CATG'C
Cuts at: 1039 1153 1653 1039
Size: 114 500 3486
Fragments arranged by size:
3486 500 114
StyI C'CwwG_G
Cuts at: 1091 1234 1588 1091
Size: 143 354 3603
Fragments arranged by size:
3603 354 143
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 130 1683 1816 2275 2484 130
Size: 1553 133 459 209 1746
Fragments arranged by size:
1746 1553 459 209 133
TatI w'GTAC_w
Cuts at: 536 3615 536
Size: 3079 1021
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 897 929 3617 3649 897
Size: 32 2688 32 1348
Fragments arranged by size:
2688 1348 32 32
Tth111I GACn'n_nGTC
Cuts at: 1897 1897
Size: 4100
XbaI T'CTAG_A
Cuts at: 1131 1131
Size: 4100
XcmI CCAnnnn_n'nnnnTGG
Cuts at: 753 1595 753
Size: 842 3258
XmaI C'CCGG_G
Cuts at: 138 200 1040 1120 138
Size: 62 840 80 3118
Fragments arranged by size:
3118 840 80 62
Enzymes that do cut:
AccI Acc65I AclI AcuI AflIII AgeI AleI AlwNI
ApaI ApaLI ApoI AseI AsiSI AvaI BamHI BanI
BanII BbeI BcgI BciVI BclI BglI BglII BlpI
Bme1580I BmgBI BmrI BplI BpmI BpuEI BsaI BsaAI
BsaBI BsaHI BsaWI BseRI BseYI BsgI BsiEI BsiHKAI
Bsp1286I BspEI BspHI BspMI BsrBI BsrDI BsrFI BssSI
BstAPI BstBI BstXI BstYI Bsu36I BtgI BtgZI BtsI
DraI DrdI EaeI EagI EarI EciI EcoICRI Eco57MI
EcoO109I EcoRI FalI FspI HaeII Hin4I HincII KasI
KpnI MluI MmeI MscI MslI MspA1I NaeI NarI
NgoMIV NspI PciI PfoI PmeI PpiI PpuMI PspOMI
PstI PvuI PvuII RsrII SacI SalI SapI SbfI
ScaI SfcI SfoI SgfI SmaI SmlI SpeI SphI
StyI TaqII TatI TstI Tth111I XbaI XcmI XmaI
Enzymes that do not cut:
AarI AatII AfeI AflII AhdI AlfI AloI AscI
AvrII BaeI BbsI BbvCI BfrBI BmtI Bpu10I BsaXI
BsiWI BsmI BsmBI BsrGI BssHII BstEII BstZ17I ClaI
CspCI DraIII EcoNI EcoRV FseI FspAI HindIII HpaI
MfeI NcoI NdeI NheI NotI NruI NsiI PacI
PasI PflMI PmlI PshAI PsiI PspXI PsrI SacII
SanDI SexAI SfiI SgrAI SnaBI SrfI SspI StuI
SwaI XhoI XmnI ZraI