Restriction map for
pF1KB7647
(Circular) (Six-base) MAPSORT of: pF1KB7647.seq Check: 7338 from: 1 to: 4097
pF1KB7647 4097 bp
With 182 enzymes: SgfI *
June 20, 2008 10:27 ..
AccI GT'mk_AC
Cuts at: 1135 1135
Size: 4097
Acc65I G'GTAC_C
Cuts at: 497 1113 497
Size: 616 3481
AclI AA'CG_TT
Cuts at: 3937 3937
Size: 4097
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 1922 2354 3101 3369 1922
Size: 432 747 268 2650
Fragments arranged by size:
2650 747 432 268
AflIII A'CryG_T
Cuts at: 1449 2553 3530 1449
Size: 1104 977 2016
Fragments arranged by size:
2016 1104 977
AgeI A'CCGG_T
Cuts at: 2485 2485
Size: 4097
AhdI GACnn_n'nnGTC
Cuts at: 144 313 144
Size: 169 3928
AlfI GCAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 811 811
Size: 4097
AlwNI CAG_nnn'CTG
Cuts at: 1402 2969 1402
Size: 1567 2530
ApaI G_GGCC'C
Cuts at: 521 852 521
Size: 331 3766
ApaLI G'TGCA_C
Cuts at: 2867 2867
Size: 4097
ApoI r'AATT_y
Cuts at: 1101 3450 3706 1101
Size: 2349 256 1492
Fragments arranged by size:
2349 1492 256
AseI AT'TA_AT
Cuts at: 20 20
Size: 4097
AsiSI GCG_AT'CGC
Cuts at: 104 104
Size: 4097
AvaI C'yCGr_G
Cuts at: 434 1117 434
Size: 683 3414
BamHI G'GATC_C
Cuts at: 1122 1122
Size: 4097
BanI G'GyrC_C
Cuts at: 497 1113 1527 1775 1810 497
Size: 616 414 248 35 2784
Fragments arranged by size:
2784 616 414 248 35
BanII G_rGCy'C
Cuts at: 343 441 521 852 1111 2141 343
Size: 98 80 331 259 1030 2299
Fragments arranged by size:
2299 1030 331 259 98 80
BbeI G_GCGC'C
Cuts at: 1531 1779 1531
Size: 248 3849
BbsI GAAGACnn'nnnn_
Cuts at: 932 932
Size: 4097
BcgI CGAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 666 700 861 895 903 937 666
Size: 34 161 34 8 34 3826
Fragments arranged by size:
3826 161 34 34 34 8
BciVI GTATCCnnnnn_n'
Cuts at: 1995 2756 4097 1995
Size: 761 1341 1995
Fragments arranged by size:
1995 1341 761
BclI T'GATC_A
Cuts at: 1617 1617
Size: 4097
BglI GCCn_nnn'nGGC
Cuts at: 176 176
Size: 4097
BglII A'GATC_T
Cuts at: 1612 3649 1612
Size: 2037 2060
BlpI GC'TnA_GC
Cuts at: 184 1209 184
Size: 1025 3072
Bme1580I G_kGCm'C
Cuts at: 521 707 852 1722 1815 2871 521
Size: 186 145 870 93 1056 1747
Fragments arranged by size:
1747 1056 870 186 145 93
BmgBI CAC'GTC
Cuts at: 165 165
Size: 4097
BmrI ACTGGGnnnn_n'
Cuts at: 815 1309 1390 1725 3869 815
Size: 494 81 335 2144 1043
Fragments arranged by size:
2144 1043 494 335 81
BplI GAGnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 1063 2464 1063
Size: 1401 2696
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 373 373
Size: 4097
Bpu10I CC'TnA_GC
Cuts at: 673 673
Size: 4097
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 1273 2152 2644 2942 1273
Size: 879 492 298 2428
Fragments arranged by size:
2428 879 492 298
BsaI GGTCTCn'nnnn_
Cuts at: 269 1068 3801 269
Size: 799 2733 565
Fragments arranged by size:
2733 799 565
BsaAI yAC'GTr
Cuts at: 984 1450 984
Size: 466 3631
BsaBI GATnn'nnATC
Cuts at: 6 1611 3648 6
Size: 1605 2037 455
Fragments arranged by size:
2037 1605 455
BsaHI Gr'CG_yC
Cuts at: 270 1528 1776 2478 3974 270
Size: 1258 248 702 1496 393
Fragments arranged by size:
1496 1258 702 393 248
BsaWI w'CCGG_w
Cuts at: 455 542 1286 1807 2485 2759 2906 3317 455
Size: 87 744 521 678 274 147 411 1235
Fragments arranged by size:
1235 744 678 521 411 274 147 87
BsaXI ACnnnnnCTCCnnnnnnn_nnn'
Cuts at: 450 480 994 1024 450
Size: 30 514 30 3523
Fragments arranged by size:
3523 514 30 30
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 720 720
Size: 4097
BseYI C'CCAG_C
Cuts at: 214 520 706 750 892 1024 1522 2280 2857
Size: 306 186 44 142 132 498 758 577
Cuts at: 2857 3353 214
Size: 496 958
Fragments arranged by size:
958 758 577 498 496 306 186 142
132 44
BsgI GTGCAGnnnnnnnnnnnnnn_nn'
Cuts at: 551 551
Size: 4097
BsiEI CG_ry'CG
Cuts at: 104 1639 1685 2893 104
Size: 1535 46 1208 1308
Fragments arranged by size:
1535 1308 1208 46
BsiHKAI G_wGCw'C
Cuts at: 1111 1889 2079 2871 1111
Size: 778 190 792 2337
Fragments arranged by size:
2337 792 778 190
Bsp1286I G_dGCh'C
Cuts at: 343 441 521 707 852 1111 1722 1815 1889
Size: 98 80 186 145 259 611 93 74
Cuts at: 1889 2079 2141 2871 343
Size: 190 62 730 1569
Fragments arranged by size:
1569 730 611 259 190 186 145 98
93 80 74 62
BspEI T'CCGG_A
Cuts at: 455 542 3317 455
Size: 87 2775 1235
Fragments arranged by size:
2775 1235 87
BspHI T'CATG_A
Cuts at: 3273 3273
Size: 4097
BspMI ACCTGCnnnn'nnnn_
Cuts at: 1147 1361 1663 2044 1147
Size: 214 302 381 3200
Fragments arranged by size:
3200 381 302 214
BsrBI CCG'CTC
Cuts at: 50 131 766 2389 2443 3927 4092 50
Size: 81 635 1623 54 1484 165 55
Fragments arranged by size:
1623 1484 635 165 81 55 54
BsrDI GCAATG_nn'
Cuts at: 2009 2009
Size: 4097
BsrFI r'CCGG_y
Cuts at: 2095 2276 2485 2095
Size: 181 209 3707
Fragments arranged by size:
3707 209 181
BssSI C'ACGA_G
Cuts at: 2368 2726 2368
Size: 358 3739
BstAPI GCAn_nnn'nTGC
Cuts at: 1474 1474
Size: 4097
BstBI TT'CG_AA
Cuts at: 2458 2458
Size: 4097
BstXI CCAn_nnnn'nTGG
Cuts at: 165 409 165
Size: 244 3853
BstYI r'GATC_y
Cuts at: 1 1122 1589 1612 1947 2193 3205 3291 3303
Size: 1121 467 23 335 246 1012 86 12
Cuts at: 3303 3649 1
Size: 346 449
Fragments arranged by size:
1121 1012 467 449 346 335 246 86
23 12
BtgZI GCGATGnnnnnnnnnn'nnnn_
Cuts at: 906 2047 2228 2391 906
Size: 1141 181 163 2612
Fragments arranged by size:
2612 1141 181 163
BtsI GCAGTG_nn'
Cuts at: 320 1340 320
Size: 1020 3077
CspCI CAAnnnnnGTGGnnnnnnnnnn_nn'
Cuts at: 309 344 309
Size: 35 4062
DraI TTT'AAA
Cuts at: 1096 1096
Size: 4097
DrdI GACnn_nn'nnGTC
Cuts at: 1803 2661 1803
Size: 858 3239
EaeI y'GGCC_r
Cuts at: 194 1682 1856 2247 2274 3619 194
Size: 1488 174 391 27 1345 672
Fragments arranged by size:
1488 1345 672 391 174 27
EagI C'GGCC_G
Cuts at: 1682 1682
Size: 4097
EarI CTCTTCn'nnn_
Cuts at: 225 348 693 2120 2330 225
Size: 123 345 1427 210 1992
Fragments arranged by size:
1992 1427 345 210 123
EciI GGCGGAnnnnnnnnn_nn'
Cuts at: 2305 2615 2761 3907 2305
Size: 310 146 1146 2495
Fragments arranged by size:
2495 1146 310 146
EcoICRI GAG'CTC
Cuts at: 1109 1109
Size: 4097
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 373 1922 2354 3101 3369 373
Size: 1549 432 747 268 1101
Fragments arranged by size:
1549 1101 747 432 268
EcoNI CCTnn'n_nnAGG
Cuts at: 484 484
Size: 4097
EcoO109I rG'GnC_Cy
Cuts at: 507 517 848 1236 507
Size: 10 331 388 3368
Fragments arranged by size:
3368 388 331 10
EcoRI G'AATT_C
Cuts at: 1101 1101
Size: 4097
FalI AAGnnnnnCTTnnnnnnnn_nnnnn'
Cuts at: 2116 2116
Size: 4097
FspI TGC'GCA
Cuts at: 1878 1878
Size: 4097
HaeII r_GCGC'y
Cuts at: 1531 1779 2801 3780 1531
Size: 248 1022 979 1848
Fragments arranged by size:
1848 1022 979 248
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 130 162 266 298 299 331 369 401 1939
Size: 32 104 32 1 32 38 32 1538
Cuts at: 1939 1969 1971 2001 130
Size: 30 2 30 2226
Fragments arranged by size:
2226 1538 104 38 32 32 32 32
30 30 2 1
HincII GTy'rAC
Cuts at: 450 1136 450
Size: 686 3411
KasI G'GCGC_C
Cuts at: 1527 1775 1527
Size: 248 3849
KpnI G_GTAC'C
Cuts at: 501 1117 501
Size: 616 3481
MluI A'CGCG_T
Cuts at: 3530 3530
Size: 4097
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 161 173 2768 2952 161
Size: 12 2595 184 1306
Fragments arranged by size:
2595 1306 184 12
MscI TGG'CCA
Cuts at: 196 1858 196
Size: 1662 2435
MslI CAynn'nnrTG
Cuts at: 163 2086 2213 163
Size: 1923 127 2047
Fragments arranged by size:
2047 1923 127
MspA1I CmG'CkG
Cuts at: 183 204 1024 1036 1208 1522 1882 2895 3140
Size: 21 820 12 172 314 360 1013 245
Cuts at: 3140 183
Size: 1140
Fragments arranged by size:
1140 1013 820 360 314 245 172 21
12
NaeI GCC'GGC
Cuts at: 2278 2278
Size: 4097
NarI GG'CG_CC
Cuts at: 1528 1776 1528
Size: 248 3849
NgoMIV G'CCGG_C
Cuts at: 2276 2276
Size: 4097
NspI r_CATG'y
Cuts at: 1150 1650 2557 1150
Size: 500 907 2690
Fragments arranged by size:
2690 907 500
PasI CC'CwG_GG
Cuts at: 1015 1015
Size: 4097
PciI A'CATG_T
Cuts at: 2553 2553
Size: 4097
PmeI GTTT'AAAC
Cuts at: 1096 1096
Size: 4097
PpiI GAACnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 3236 3268 3236
Size: 32 4065
PpuMI rG'GwC_Cy
Cuts at: 507 507
Size: 4097
PspOMI G'GGCC_C
Cuts at: 517 848 517
Size: 331 3766
PstI C_TGCA'G
Cuts at: 938 1144 1829 938
Size: 206 685 3206
Fragments arranged by size:
3206 685 206
PvuI CG_AT'CG
Cuts at: 104 104
Size: 4097
PvuII CAG'CTG
Cuts at: 183 1024 1522 1882 183
Size: 841 498 360 2398
Fragments arranged by size:
2398 841 498 360
RsrII CG'GwC_CG
Cuts at: 2292 2292
Size: 4097
SacI G_AGCT'C
Cuts at: 1111 1111
Size: 4097
SalI G'TCGA_C
Cuts at: 1134 1134
Size: 4097
SapI GCTCTTCn'nnn_
Cuts at: 225 348 2120 2330 225
Size: 123 1772 210 1992
Fragments arranged by size:
1992 1772 210 123
SbfI CC_TGCA'GG
Cuts at: 1144 1144
Size: 4097
ScaI AGT'ACT
Cuts at: 3614 3614
Size: 4097
SfcI C'TryA_G
Cuts at: 33 934 965 1140 1825 2818 3009 33
Size: 901 31 175 685 993 191 1121
Fragments arranged by size:
1121 993 901 685 191 175 31
SfiI GGCCn_nnn'nGGCC
Cuts at: 176 176
Size: 4097
SfoI GGC'GCC
Cuts at: 1529 1777 1529
Size: 248 3849
SgfI GCG_AT'CGC
Cuts at: 104 104
Size: 4097
SmaI CCC'GGG
Cuts at: 1119 1119
Size: 4097
SmlI C'TyrA_G
Cuts at: 1252 2167 2659 2921 1252
Size: 915 492 262 2428
Fragments arranged by size:
2428 915 492 262
SpeI A'CTAG_T
Cuts at: 67 67
Size: 4097
SphI G_CATG'C
Cuts at: 1150 1650 1150
Size: 500 3597
StyI C'CwwG_G
Cuts at: 119 1231 1585 119
Size: 1112 354 2631
Fragments arranged by size:
2631 1112 354
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 1680 1813 2272 2481 1680
Size: 133 459 209 3296
Fragments arranged by size:
3296 459 209 133
TatI w'GTAC_w
Cuts at: 3612 3612
Size: 4097
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 3614 3646 3614
Size: 32 4065
Tth111I GACn'n_nGTC
Cuts at: 538 1894 538
Size: 1356 2741
XbaI T'CTAG_A
Cuts at: 1128 1128
Size: 4097
XcmI CCAnnnn_n'nnnnTGG
Cuts at: 409 612 1592 409
Size: 203 980 2914
Fragments arranged by size:
2914 980 203
XmaI C'CCGG_G
Cuts at: 1117 1117
Size: 4097
Enzymes that do cut:
AccI Acc65I AclI AcuI AflIII AgeI AhdI AlfI
AlwNI ApaI ApaLI ApoI AseI AsiSI AvaI BamHI
BanI BanII BbeI BbsI BcgI BciVI BclI BglI
BglII BlpI Bme1580I BmgBI BmrI BplI BpmI Bpu10I
BpuEI BsaI BsaAI BsaBI BsaHI BsaWI BsaXI BseRI
BseYI BsgI BsiEI BsiHKAI Bsp1286I BspEI BspHI BspMI
BsrBI BsrDI BsrFI BssSI BstAPI BstBI BstXI BstYI
BtgZI BtsI CspCI DraI DrdI EaeI EagI EarI
EciI EcoICRI Eco57MI EcoNI EcoO109I EcoRI FalI FspI
HaeII Hin4I HincII KasI KpnI MluI MmeI MscI
MslI MspA1I NaeI NarI NgoMIV NspI PasI PciI
PmeI PpiI PpuMI PspOMI PstI PvuI PvuII RsrII
SacI SalI SapI SbfI ScaI SfcI SfiI SfoI
SgfI SmaI SmlI SpeI SphI StyI TaqII TatI
TstI Tth111I XbaI XcmI XmaI
Enzymes that do not cut:
AarI AatII AfeI AflII AleI AloI AscI AvrII
BaeI BbvCI BfrBI BmtI BsiWI BsmI BsmBI BsrGI
BssHII BstEII BstZ17I Bsu36I BtgI ClaI DraIII EcoRV
FseI FspAI HindIII HpaI MfeI NcoI NdeI NheI
NotI NruI NsiI PacI PflMI PfoI PmlI PshAI
PsiI PspXI PsrI SacII SanDI SexAI SgrAI SnaBI
SrfI SspI StuI SwaI XhoI XmnI ZraI