Restriction map for
pF1KB7633
(Circular) (Six-base) MAPSORT of: pF1KB7633.seq Check: 258 from: 1 to: 4076
pF1KB7633 4076 bp
With 182 enzymes: SgfI *
June 26, 2008 10:49 ..
AccI GT'mk_AC
Cuts at: 1114 1114
Size: 4076
Acc65I G'GTAC_C
Cuts at: 1092 1092
Size: 4076
AclI AA'CG_TT
Cuts at: 3916 3916
Size: 4076
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 340 676 825 1901 2333 3080 3348 340
Size: 336 149 1076 432 747 268 1068
Fragments arranged by size:
1076 1068 747 432 336 268 149
AflII C'TTAA_G
Cuts at: 523 775 523
Size: 252 3824
AflIII A'CryG_T
Cuts at: 699 951 1428 2532 3509 699
Size: 252 477 1104 977 1266
Fragments arranged by size:
1266 1104 977 477 252
AgeI A'CCGG_T
Cuts at: 2464 2464
Size: 4076
AleI CACnn'nnGTG
Cuts at: 884 884
Size: 4076
AlwNI CAG_nnn'CTG
Cuts at: 491 743 1381 2948 491
Size: 252 638 1567 1619
Fragments arranged by size:
1619 1567 638 252
ApaLI G'TGCA_C
Cuts at: 613 865 2846 613
Size: 252 1981 1843
Fragments arranged by size:
1981 1843 252
ApoI r'AATT_y
Cuts at: 338 930 1080 3429 3685 338
Size: 592 150 2349 256 729
Fragments arranged by size:
2349 729 592 256 150
AseI AT'TA_AT
Cuts at: 20 20
Size: 4076
AsiSI GCG_AT'CGC
Cuts at: 104 104
Size: 4076
AvaI C'yCGr_G
Cuts at: 1096 1096
Size: 4076
BamHI G'GATC_C
Cuts at: 1101 1101
Size: 4076
BanI G'GyrC_C
Cuts at: 1092 1506 1754 1789 1092
Size: 414 248 35 3379
Fragments arranged by size:
3379 414 248 35
BanII G_rGCy'C
Cuts at: 601 1090 2120 601
Size: 489 1030 2557
Fragments arranged by size:
2557 1030 489
BbeI G_GCGC'C
Cuts at: 1510 1758 1510
Size: 248 3828
BbsI GAAGACnn'nnnn_
Cuts at: 328 509 328
Size: 181 3895
BciVI GTATCCnnnnn_n'
Cuts at: 1974 2735 4076 1974
Size: 761 1341 1974
Fragments arranged by size:
1974 1341 761
BclI T'GATC_A
Cuts at: 570 1596 570
Size: 1026 3050
BglII A'GATC_T
Cuts at: 538 1591 3628 538
Size: 1053 2037 986
Fragments arranged by size:
2037 1053 986
BlpI GC'TnA_GC
Cuts at: 1188 1188
Size: 4076
Bme1580I G_kGCm'C
Cuts at: 617 869 1701 1794 2850 617
Size: 252 832 93 1056 1843
Fragments arranged by size:
1843 1056 832 252 93
BmrI ACTGGGnnnn_n'
Cuts at: 372 1288 1369 1704 3848 372
Size: 916 81 335 2144 600
Fragments arranged by size:
2144 916 600 335 81
BplI GAGnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 2443 2443
Size: 4076
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 259 485 569 653 737 821 989 259
Size: 226 84 84 84 84 168 3346
Fragments arranged by size:
3346 226 168 84 84 84 84
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 1252 2131 2623 2921 1252
Size: 879 492 298 2407
Fragments arranged by size:
2407 879 492 298
BsaI GGTCTCn'nnnn_
Cuts at: 3780 3780
Size: 4076
BsaAI yAC'GTr
Cuts at: 954 1429 954
Size: 475 3601
BsaBI GATnn'nnATC
Cuts at: 6 1590 3627 6
Size: 1584 2037 455
Fragments arranged by size:
2037 1584 455
BsaHI Gr'CG_yC
Cuts at: 1507 1755 2457 3953 1507
Size: 248 702 1496 1630
Fragments arranged by size:
1630 1496 702 248
BsaWI w'CCGG_w
Cuts at: 1265 1786 2464 2738 2885 3296 1265
Size: 521 678 274 147 411 2045
Fragments arranged by size:
2045 678 521 411 274 147
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 199 199
Size: 4076
BseYI C'CCAG_C
Cuts at: 1501 2259 2836 3332 1501
Size: 758 577 496 2245
Fragments arranged by size:
2245 758 577 496
BsgI GTGCAGnnnnnnnnnnnnnn_nn'
Cuts at: 377 377
Size: 4076
BsiEI CG_ry'CG
Cuts at: 104 1618 1664 2872 104
Size: 1514 46 1208 1308
Fragments arranged by size:
1514 1308 1208 46
BsiHKAI G_wGCw'C
Cuts at: 601 617 869 1090 1868 2058 2850 601
Size: 16 252 221 778 190 792 1827
Fragments arranged by size:
1827 792 778 252 221 190 16
BsmI GAATG_Cn'
Cuts at: 487 739 487
Size: 252 3824
Bsp1286I G_dGCh'C
Cuts at: 601 617 869 1090 1701 1794 1868 2058 2120
Size: 16 252 221 611 93 74 190 62
Cuts at: 2120 2850 601
Size: 730 1827
Fragments arranged by size:
1827 730 611 252 221 190 93 74
62 16
BspEI T'CCGG_A
Cuts at: 3296 3296
Size: 4076
BspHI T'CATG_A
Cuts at: 3252 3252
Size: 4076
BspMI ACCTGCnnnn'nnnn_
Cuts at: 1126 1340 1642 2023 1126
Size: 214 302 381 3179
Fragments arranged by size:
3179 381 302 214
BsrBI CCG'CTC
Cuts at: 50 2368 2422 3906 4071 50
Size: 2318 54 1484 165 55
Fragments arranged by size:
2318 1484 165 55 54
BsrDI GCAATG_nn'
Cuts at: 193 1988 193
Size: 1795 2281
BsrFI r'CCGG_y
Cuts at: 2074 2255 2464 2074
Size: 181 209 3686
Fragments arranged by size:
3686 209 181
BssSI C'ACGA_G
Cuts at: 2347 2705 2347
Size: 358 3718
BstAPI GCAn_nnn'nTGC
Cuts at: 1453 1453
Size: 4076
BstBI TT'CG_AA
Cuts at: 2437 2437
Size: 4076
BstYI r'GATC_y
Cuts at: 1 538 1101 1568 1591 1926 2172 3184 3270
Size: 537 563 467 23 335 246 1012 86
Cuts at: 3270 3282 3628 1
Size: 12 346 449
Fragments arranged by size:
1012 563 537 467 449 346 335 246
86 23 12
Bsu36I CC'TnA_GG
Cuts at: 943 943
Size: 4076
BtgI C'CryG_G
Cuts at: 107 107
Size: 4076
BtgZI GCGATGnnnnnnnnnn'nnnn_
Cuts at: 2026 2207 2370 2026
Size: 181 163 3732
Fragments arranged by size:
3732 181 163
BtsI GCAGTG_nn'
Cuts at: 173 492 744 1319 173
Size: 319 252 575 2930
Fragments arranged by size:
2930 575 319 252
ClaI AT'CG_AT
Cuts at: 761 761
Size: 4076
DraI TTT'AAA
Cuts at: 1075 1075
Size: 4076
DrdI GACnn_nn'nnGTC
Cuts at: 132 1782 2640 132
Size: 1650 858 1568
Fragments arranged by size:
1650 1568 858
EaeI y'GGCC_r
Cuts at: 1053 1661 1835 2226 2253 3598 1053
Size: 608 174 391 27 1345 1531
Fragments arranged by size:
1531 1345 608 391 174 27
EagI C'GGCC_G
Cuts at: 1661 1661
Size: 4076
EarI CTCTTCn'nnn_
Cuts at: 2099 2309 2099
Size: 210 3866
EciI GGCGGAnnnnnnnnn_nn'
Cuts at: 2284 2594 2740 3886 2284
Size: 310 146 1146 2474
Fragments arranged by size:
2474 1146 310 146
EcoICRI GAG'CTC
Cuts at: 599 1088 599
Size: 489 3587
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 259 340 485 569 653 676 737 821 825
Size: 81 145 84 84 23 61 84 4
Cuts at: 825 989 1901 2333 3080 3348 259
Size: 164 912 432 747 268 987
Fragments arranged by size:
987 912 747 432 268 164 145 84
84 84 81 61 23 4
EcoO109I rG'GnC_Cy
Cuts at: 1215 1215
Size: 4076
EcoRI G'AATT_C
Cuts at: 930 1080 930
Size: 150 3926
FalI AAGnnnnnCTTnnnnnnnn_nnnnn'
Cuts at: 2095 2095
Size: 4076
FspI TGC'GCA
Cuts at: 1857 1857
Size: 4076
HaeII r_GCGC'y
Cuts at: 1510 1758 2780 3759 1510
Size: 248 1022 979 1827
Fragments arranged by size:
1827 1022 979 248
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 588 620 754 786 1918 1948 1950 1980 588
Size: 32 134 32 1132 30 2 30 2684
Fragments arranged by size:
2684 1132 134 32 32 30 30 2
HincII GTy'rAC
Cuts at: 1115 1115
Size: 4076
KasI G'GCGC_C
Cuts at: 1506 1754 1506
Size: 248 3828
KpnI G_GTAC'C
Cuts at: 1096 1096
Size: 4076
MfeI C'AATT_G
Cuts at: 392 392
Size: 4076
MluI A'CGCG_T
Cuts at: 3509 3509
Size: 4076
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 2747 2931 2747
Size: 184 3892
MscI TGG'CCA
Cuts at: 1055 1837 1055
Size: 782 3294
MslI CAynn'nnrTG
Cuts at: 608 884 2065 2192 608
Size: 276 1181 127 2492
Fragments arranged by size:
2492 1181 276 127
MspA1I CmG'CkG
Cuts at: 318 1187 1501 1861 2874 3119 318
Size: 869 314 360 1013 245 1275
Fragments arranged by size:
1275 1013 869 360 314 245
NaeI GCC'GGC
Cuts at: 2257 2257
Size: 4076
NarI GG'CG_CC
Cuts at: 1507 1755 1507
Size: 248 3828
NcoI C'CATG_G
Cuts at: 107 107
Size: 4076
NgoMIV G'CCGG_C
Cuts at: 2255 2255
Size: 4076
NspI r_CATG'y
Cuts at: 703 1129 1629 2536 703
Size: 426 500 907 2243
Fragments arranged by size:
2243 907 500 426
PciI A'CATG_T
Cuts at: 699 2532 699
Size: 1833 2243
PflMI CCAn_nnn'nTGG
Cuts at: 162 162
Size: 4076
PfoI T'CCnGG_A
Cuts at: 155 155
Size: 4076
PmeI GTTT'AAAC
Cuts at: 1075 1075
Size: 4076
PmlI CAC'GTG
Cuts at: 954 954
Size: 4076
PpiI GAACnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 244 276 3215 3247 244
Size: 32 2939 32 1073
Fragments arranged by size:
2939 1073 32 32
PsiI TTA'TAA
Cuts at: 983 1030 983
Size: 47 4029
PstI C_TGCA'G
Cuts at: 117 283 1123 1808 117
Size: 166 840 685 2385
Fragments arranged by size:
2385 840 685 166
PvuI CG_AT'CG
Cuts at: 104 104
Size: 4076
PvuII CAG'CTG
Cuts at: 318 1501 1861 318
Size: 1183 360 2533
Fragments arranged by size:
2533 1183 360
RsrII CG'GwC_CG
Cuts at: 2271 2271
Size: 4076
SacI G_AGCT'C
Cuts at: 601 1090 601
Size: 489 3587
SalI G'TCGA_C
Cuts at: 1113 1113
Size: 4076
SapI GCTCTTCn'nnn_
Cuts at: 2099 2309 2099
Size: 210 3866
SbfI CC_TGCA'GG
Cuts at: 1123 1123
Size: 4076
ScaI AGT'ACT
Cuts at: 3593 3593
Size: 4076
SexAI A'CCwGG_T
Cuts at: 130 130
Size: 4076
SfcI C'TryA_G
Cuts at: 33 113 279 1119 1804 2797 2988 33
Size: 80 166 840 685 993 191 1121
Fragments arranged by size:
1121 993 840 685 191 166 80
SfoI GGC'GCC
Cuts at: 1508 1756 1508
Size: 248 3828
SgfI GCG_AT'CGC
Cuts at: 104 104
Size: 4076
SmaI CCC'GGG
Cuts at: 1098 1098
Size: 4076
SmlI C'TyrA_G
Cuts at: 523 775 1231 2146 2638 2900 523
Size: 252 456 915 492 262 1699
Fragments arranged by size:
1699 915 492 456 262 252
SpeI A'CTAG_T
Cuts at: 67 67
Size: 4076
SphI G_CATG'C
Cuts at: 1129 1629 1129
Size: 500 3576
StuI AGG'CCT
Cuts at: 922 922
Size: 4076
StyI C'CwwG_G
Cuts at: 107 299 1210 1564 107
Size: 192 911 354 2619
Fragments arranged by size:
2619 911 354 192
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 1659 1792 2251 2460 1659
Size: 133 459 209 3275
Fragments arranged by size:
3275 459 209 133
TatI w'GTAC_w
Cuts at: 3591 3591
Size: 4076
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 3593 3625 3593
Size: 32 4044
Tth111I GACn'n_nGTC
Cuts at: 287 1873 287
Size: 1586 2490
XbaI T'CTAG_A
Cuts at: 1107 1107
Size: 4076
XcmI CCAnnnn_n'nnnnTGG
Cuts at: 1571 1571
Size: 4076
XmaI C'CCGG_G
Cuts at: 1096 1096
Size: 4076
XmnI GAAnn'nnTTC
Cuts at: 502 670 502
Size: 168 3908
Enzymes that do cut:
AccI Acc65I AclI AcuI AflII AflIII AgeI AleI
AlwNI ApaLI ApoI AseI AsiSI AvaI BamHI BanI
BanII BbeI BbsI BciVI BclI BglII BlpI Bme1580I
BmrI BplI BpmI BpuEI BsaI BsaAI BsaBI BsaHI
BsaWI BseRI BseYI BsgI BsiEI BsiHKAI BsmI Bsp1286I
BspEI BspHI BspMI BsrBI BsrDI BsrFI BssSI BstAPI
BstBI BstYI Bsu36I BtgI BtgZI BtsI ClaI DraI
DrdI EaeI EagI EarI EciI EcoICRI Eco57MI EcoO109I
EcoRI FalI FspI HaeII Hin4I HincII KasI KpnI
MfeI MluI MmeI MscI MslI MspA1I NaeI NarI
NcoI NgoMIV NspI PciI PflMI PfoI PmeI PmlI
PpiI PsiI PstI PvuI PvuII RsrII SacI SalI
SapI SbfI ScaI SexAI SfcI SfoI SgfI SmaI
SmlI SpeI SphI StuI StyI TaqII TatI TstI
Tth111I XbaI XcmI XmaI XmnI
Enzymes that do not cut:
AarI AatII AfeI AhdI AlfI AloI ApaI AscI
AvrII BaeI BbvCI BcgI BfrBI BglI BmgBI BmtI
Bpu10I BsaXI BsiWI BsmBI BsrGI BssHII BstEII BstXI
BstZ17I CspCI DraIII EcoNI EcoRV FseI FspAI HindIII
HpaI NdeI NheI NotI NruI NsiI PacI PasI
PpuMI PshAI PspOMI PspXI PsrI SacII SanDI SfiI
SgrAI SnaBI SrfI SspI SwaI XhoI ZraI