Restriction map for
pF1KB7629
(Circular) (Six-base) MAPSORT of: pF1KB7629.seq Check: 1885 from: 1 to: 4070
pF1KB7629 4070 bp
With 182 enzymes: SgfI *
October 7, 2009 14:24 ..
AccI GT'mk_AC
Cuts at: 1108 1108
Size: 4070
Acc65I G'GTAC_C
Cuts at: 1086 1086
Size: 4070
AclI AA'CG_TT
Cuts at: 3910 3910
Size: 4070
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 262 406 502 1895 2327 3074 3342 262
Size: 144 96 1393 432 747 268 990
Fragments arranged by size:
1393 990 747 432 268 144 96
AfeI AGC'GCT
Cuts at: 773 773
Size: 4070
AflIII A'CryG_T
Cuts at: 1422 2526 3503 1422
Size: 1104 977 1989
Fragments arranged by size:
1989 1104 977
AgeI A'CCGG_T
Cuts at: 2458 2458
Size: 4070
AlfI GCAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 790 790
Size: 4070
AlwNI CAG_nnn'CTG
Cuts at: 442 1375 2942 442
Size: 933 1567 1570
Fragments arranged by size:
1570 1567 933
ApaLI G'TGCA_C
Cuts at: 2840 2840
Size: 4070
ApoI r'AATT_y
Cuts at: 172 354 520 646 1074 3423 3679 172
Size: 182 166 126 428 2349 256 563
Fragments arranged by size:
2349 563 428 256 182 166 126
AseI AT'TA_AT
Cuts at: 20 20
Size: 4070
AsiSI GCG_AT'CGC
Cuts at: 104 104
Size: 4070
AvaI C'yCGr_G
Cuts at: 749 1090 749
Size: 341 3729
BamHI G'GATC_C
Cuts at: 1095 1095
Size: 4070
BanI G'GyrC_C
Cuts at: 1086 1500 1748 1783 1086
Size: 414 248 35 3373
Fragments arranged by size:
3373 414 248 35
BanII G_rGCy'C
Cuts at: 317 639 1084 2114 317
Size: 322 445 1030 2273
Fragments arranged by size:
2273 1030 445 322
BbeI G_GCGC'C
Cuts at: 1504 1752 1504
Size: 248 3822
BbsI GAAGACnn'nnnn_
Cuts at: 250 368 970 250
Size: 118 602 3350
Fragments arranged by size:
3350 602 118
BciVI GTATCCnnnnn_n'
Cuts at: 1968 2729 4070 1968
Size: 761 1341 1968
Fragments arranged by size:
1968 1341 761
BclI T'GATC_A
Cuts at: 1590 1590
Size: 4070
BglI GCCn_nnn'nGGC
Cuts at: 832 832
Size: 4070
BglII A'GATC_T
Cuts at: 1585 3622 1585
Size: 2037 2033
BlpI GC'TnA_GC
Cuts at: 763 1182 763
Size: 419 3651
Bme1580I G_kGCm'C
Cuts at: 1695 1788 2844 1695
Size: 93 1056 2921
Fragments arranged by size:
2921 1056 93
BmrI ACTGGGnnnn_n'
Cuts at: 690 1282 1363 1698 3842 690
Size: 592 81 335 2144 918
Fragments arranged by size:
2144 918 592 335 81
BplI GAGnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 2437 2437
Size: 4070
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 514 653 514
Size: 139 3931
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 1246 2125 2617 2915 1246
Size: 879 492 298 2401
Fragments arranged by size:
2401 879 492 298
BsaI GGTCTCn'nnnn_
Cuts at: 3774 3774
Size: 4070
BsaAI yAC'GTr
Cuts at: 1423 1423
Size: 4070
BsaBI GATnn'nnATC
Cuts at: 6 1584 3621 6
Size: 1578 2037 455
Fragments arranged by size:
2037 1578 455
BsaHI Gr'CG_yC
Cuts at: 967 1501 1749 2451 3947 967
Size: 534 248 702 1496 1090
Fragments arranged by size:
1496 1090 702 534 248
BsaWI w'CCGG_w
Cuts at: 1259 1780 2458 2732 2879 3290 1259
Size: 521 678 274 147 411 2039
Fragments arranged by size:
2039 678 521 411 274 147
BsaXI ACnnnnnCTCCnnnnnnn_nnn'
Cuts at: 268 298 268
Size: 30 4040
BseYI C'CCAG_C
Cuts at: 958 1495 2253 2830 3326 958
Size: 537 758 577 496 1702
Fragments arranged by size:
1702 758 577 537 496
BsiEI CG_ry'CG
Cuts at: 104 1612 1658 2866 104
Size: 1508 46 1208 1308
Fragments arranged by size:
1508 1308 1208 46
BsiHKAI G_wGCw'C
Cuts at: 317 1084 1862 2052 2844 317
Size: 767 778 190 792 1543
Fragments arranged by size:
1543 792 778 767 190
Bsp1286I G_dGCh'C
Cuts at: 317 639 1084 1695 1788 1862 2052 2114 2844
Size: 322 445 611 93 74 190 62 730
Cuts at: 2844 317
Size: 1543
Fragments arranged by size:
1543 730 611 445 322 190 93 74
62
BspEI T'CCGG_A
Cuts at: 3290 3290
Size: 4070
BspHI T'CATG_A
Cuts at: 3246 3246
Size: 4070
BspMI ACCTGCnnnn'nnnn_
Cuts at: 1120 1334 1636 2017 1120
Size: 214 302 381 3173
Fragments arranged by size:
3173 381 302 214
BsrBI CCG'CTC
Cuts at: 50 2362 2416 3900 4065 50
Size: 2312 54 1484 165 55
Fragments arranged by size:
2312 1484 165 55 54
BsrDI GCAATG_nn'
Cuts at: 162 1982 162
Size: 1820 2250
BsrFI r'CCGG_y
Cuts at: 2068 2249 2458 2068
Size: 181 209 3680
Fragments arranged by size:
3680 209 181
BssSI C'ACGA_G
Cuts at: 2341 2699 2341
Size: 358 3712
BstAPI GCAn_nnn'nTGC
Cuts at: 1447 1447
Size: 4070
BstBI TT'CG_AA
Cuts at: 2431 2431
Size: 4070
BstYI r'GATC_y
Cuts at: 1 237 936 975 1095 1562 1585 1920 2166
Size: 236 699 39 120 467 23 335 246
Cuts at: 2166 3178 3264 3276 3622 1
Size: 1012 86 12 346 449
Fragments arranged by size:
1012 699 467 449 346 335 246 236
120 86 39 23 12
Bsu36I CC'TnA_GG
Cuts at: 182 182
Size: 4070
BtgI C'CryG_G
Cuts at: 690 690
Size: 4070
BtgZI GCGATGnnnnnnnnnn'nnnn_
Cuts at: 2020 2201 2364 2020
Size: 181 163 3726
Fragments arranged by size:
3726 181 163
BtsI GCAGTG_nn'
Cuts at: 1313 1313
Size: 4070
DraI TTT'AAA
Cuts at: 713 1069 713
Size: 356 3714
DraIII CAC_nnn'GTG
Cuts at: 264 264
Size: 4070
DrdI GACnn_nn'nnGTC
Cuts at: 1776 2634 1776
Size: 858 3212
EaeI y'GGCC_r
Cuts at: 795 1655 1829 2220 2247 3592 795
Size: 860 174 391 27 1345 1273
Fragments arranged by size:
1345 1273 860 391 174 27
EagI C'GGCC_G
Cuts at: 1655 1655
Size: 4070
EarI CTCTTCn'nnn_
Cuts at: 581 2093 2303 581
Size: 1512 210 2348
Fragments arranged by size:
2348 1512 210
EciI GGCGGAnnnnnnnnn_nn'
Cuts at: 2278 2588 2734 3880 2278
Size: 310 146 1146 2468
Fragments arranged by size:
2468 1146 310 146
EcoICRI GAG'CTC
Cuts at: 315 1082 315
Size: 767 3303
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 262 406 502 514 653 1895 2327 3074 3342
Size: 144 96 12 139 1242 432 747 268
Cuts at: 3342 262
Size: 990
Fragments arranged by size:
1242 990 747 432 268 144 139 96
12
EcoNI CCTnn'n_nnAGG
Cuts at: 181 181
Size: 4070
EcoO109I rG'GnC_Cy
Cuts at: 1209 1209
Size: 4070
EcoRI G'AATT_C
Cuts at: 172 646 1074 172
Size: 474 428 3168
Fragments arranged by size:
3168 474 428
FalI AAGnnnnnCTTnnnnnnnn_nnnnn'
Cuts at: 2089 2089
Size: 4070
FspI TGC'GCA
Cuts at: 1851 1851
Size: 4070
HaeII r_GCGC'y
Cuts at: 749 775 1504 1752 2774 3753 749
Size: 26 729 248 1022 979 1066
Fragments arranged by size:
1066 1022 979 729 248 26
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 626 658 1912 1942 1944 1974 626
Size: 32 1254 30 2 30 2722
Fragments arranged by size:
2722 1254 32 30 30 2
HincII GTy'rAC
Cuts at: 1109 1109
Size: 4070
HindIII A'AGCT_T
Cuts at: 346 346
Size: 4070
KasI G'GCGC_C
Cuts at: 1500 1748 1500
Size: 248 3822
KpnI G_GTAC'C
Cuts at: 1090 1090
Size: 4070
MfeI C'AATT_G
Cuts at: 608 608
Size: 4070
MluI A'CGCG_T
Cuts at: 3503 3503
Size: 4070
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 936 2741 2925 936
Size: 1805 184 2081
Fragments arranged by size:
2081 1805 184
MscI TGG'CCA
Cuts at: 797 1831 797
Size: 1034 3036
MslI CAynn'nnrTG
Cuts at: 2059 2186 2059
Size: 127 3943
MspA1I CmG'CkG
Cuts at: 1181 1495 1855 2868 3113 1181
Size: 314 360 1013 245 2138
Fragments arranged by size:
2138 1013 360 314 245
NaeI GCC'GGC
Cuts at: 2251 2251
Size: 4070
NarI GG'CG_CC
Cuts at: 1501 1749 1501
Size: 248 3822
NgoMIV G'CCGG_C
Cuts at: 2249 2249
Size: 4070
NspI r_CATG'y
Cuts at: 590 781 1123 1623 2530 590
Size: 191 342 500 907 2130
Fragments arranged by size:
2130 907 500 342 191
PasI CC'CwG_GG
Cuts at: 270 270
Size: 4070
PciI A'CATG_T
Cuts at: 2526 2526
Size: 4070
PflMI CCAn_nnn'nTGG
Cuts at: 680 680
Size: 4070
PmeI GTTT'AAAC
Cuts at: 1069 1069
Size: 4070
PpiI GAACnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 3209 3241 3209
Size: 32 4038
PstI C_TGCA'G
Cuts at: 1117 1802 1117
Size: 685 3385
PvuI CG_AT'CG
Cuts at: 104 104
Size: 4070
PvuII CAG'CTG
Cuts at: 1495 1855 1495
Size: 360 3710
RsrII CG'GwC_CG
Cuts at: 2265 2265
Size: 4070
SacI G_AGCT'C
Cuts at: 317 1084 317
Size: 767 3303
SalI G'TCGA_C
Cuts at: 1107 1107
Size: 4070
SapI GCTCTTCn'nnn_
Cuts at: 581 2093 2303 581
Size: 1512 210 2348
Fragments arranged by size:
2348 1512 210
SbfI CC_TGCA'GG
Cuts at: 1117 1117
Size: 4070
ScaI AGT'ACT
Cuts at: 3587 3587
Size: 4070
SfcI C'TryA_G
Cuts at: 33 1113 1798 2791 2982 33
Size: 1080 685 993 191 1121
Fragments arranged by size:
1121 1080 993 685 191
SfoI GGC'GCC
Cuts at: 1502 1750 1502
Size: 248 3822
SgfI GCG_AT'CGC
Cuts at: 104 104
Size: 4070
SmaI CCC'GGG
Cuts at: 1092 1092
Size: 4070
SmlI C'TyrA_G
Cuts at: 1225 2140 2632 2894 1225
Size: 915 492 262 2401
Fragments arranged by size:
2401 915 492 262
SpeI A'CTAG_T
Cuts at: 67 67
Size: 4070
SphI G_CATG'C
Cuts at: 590 1123 1623 590
Size: 533 500 3037
Fragments arranged by size:
3037 533 500
SspI AAT'ATT
Cuts at: 1013 1013
Size: 4070
StyI C'CwwG_G
Cuts at: 1204 1558 1204
Size: 354 3716
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 1653 1786 2245 2454 1653
Size: 133 459 209 3269
Fragments arranged by size:
3269 459 209 133
TatI w'GTAC_w
Cuts at: 3585 3585
Size: 4070
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 268 300 3587 3619 268
Size: 32 3287 32 719
Fragments arranged by size:
3287 719 32 32
Tth111I GACn'n_nGTC
Cuts at: 1867 1867
Size: 4070
XbaI T'CTAG_A
Cuts at: 1101 1101
Size: 4070
XcmI CCAnnnn_n'nnnnTGG
Cuts at: 1565 1565
Size: 4070
XmaI C'CCGG_G
Cuts at: 1090 1090
Size: 4070
XmnI GAAnn'nnTTC
Cuts at: 176 176
Size: 4070
Enzymes that do cut:
AccI Acc65I AclI AcuI AfeI AflIII AgeI AlfI
AlwNI ApaLI ApoI AseI AsiSI AvaI BamHI BanI
BanII BbeI BbsI BciVI BclI BglI BglII BlpI
Bme1580I BmrI BplI BpmI BpuEI BsaI BsaAI BsaBI
BsaHI BsaWI BsaXI BseYI BsiEI BsiHKAI Bsp1286I BspEI
BspHI BspMI BsrBI BsrDI BsrFI BssSI BstAPI BstBI
BstYI Bsu36I BtgI BtgZI BtsI DraI DraIII DrdI
EaeI EagI EarI EciI EcoICRI Eco57MI EcoNI EcoO109I
EcoRI FalI FspI HaeII Hin4I HincII HindIII KasI
KpnI MfeI MluI MmeI MscI MslI MspA1I NaeI
NarI NgoMIV NspI PasI PciI PflMI PmeI PpiI
PstI PvuI PvuII RsrII SacI SalI SapI SbfI
ScaI SfcI SfoI SgfI SmaI SmlI SpeI SphI
SspI StyI TaqII TatI TstI Tth111I XbaI XcmI
XmaI XmnI
Enzymes that do not cut:
AarI AatII AflII AhdI AleI AloI ApaI AscI
AvrII BaeI BbvCI BcgI BfrBI BmgBI BmtI Bpu10I
BseRI BsgI BsiWI BsmI BsmBI BsrGI BssHII BstEII
BstXI BstZ17I ClaI CspCI EcoRV FseI FspAI HpaI
NcoI NdeI NheI NotI NruI NsiI PacI PfoI
PmlI PpuMI PshAI PsiI PspOMI PspXI PsrI SacII
SanDI SexAI SfiI SgrAI SnaBI SrfI StuI SwaI
XhoI ZraI