Restriction map for
pF1KB7628
(Circular) (Six-base) MAPSORT of: pF1KB7628.seq Check: 4973 from: 1 to: 4232
pF1KB7628 4232 bp
With 182 enzymes: SgfI *
June 20, 2008 10:27 ..
AarI CACCTGCnnnn'nnnn_
Cuts at: 450 450
Size: 4232
AccI GT'mk_AC
Cuts at: 1270 1270
Size: 4232
Acc65I G'GTAC_C
Cuts at: 171 1248 171
Size: 1077 3155
AclI AA'CG_TT
Cuts at: 4072 4072
Size: 4232
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 626 2057 2489 3236 3504 626
Size: 1431 432 747 268 1354
Fragments arranged by size:
1431 1354 747 432 268
AfeI AGC'GCT
Cuts at: 385 385
Size: 4232
AflIII A'CryG_T
Cuts at: 1584 2688 3665 1584
Size: 1104 977 2151
Fragments arranged by size:
2151 1104 977
AgeI A'CCGG_T
Cuts at: 2620 2620
Size: 4232
AhdI GACnn_n'nnGTC
Cuts at: 655 655
Size: 4232
AlwNI CAG_nnn'CTG
Cuts at: 1537 3104 1537
Size: 1567 2665
ApaLI G'TGCA_C
Cuts at: 3002 3002
Size: 4232
ApoI r'AATT_y
Cuts at: 1236 3585 3841 1236
Size: 2349 256 1627
Fragments arranged by size:
2349 1627 256
AseI AT'TA_AT
Cuts at: 20 20
Size: 4232
AsiSI GCG_AT'CGC
Cuts at: 104 104
Size: 4232
AvaI C'yCGr_G
Cuts at: 166 240 850 1119 1252 166
Size: 74 610 269 133 3146
Fragments arranged by size:
3146 610 269 133 74
BamHI G'GATC_C
Cuts at: 856 1257 856
Size: 401 3831
BanI G'GyrC_C
Cuts at: 171 298 598 1248 1662 1910 1945 171
Size: 127 300 650 414 248 35 2458
Fragments arranged by size:
2458 650 414 300 248 127 35
BanII G_rGCy'C
Cuts at: 964 1187 1246 2276 964
Size: 223 59 1030 2920
Fragments arranged by size:
2920 1030 223 59
BbeI G_GCGC'C
Cuts at: 1666 1914 1666
Size: 248 3984
BcgI CGAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 90 124 411 445 90
Size: 34 287 34 3877
Fragments arranged by size:
3877 287 34 34
BciVI GTATCCnnnnn_n'
Cuts at: 2130 2891 4232 2130
Size: 761 1341 2130
Fragments arranged by size:
2130 1341 761
BclI T'GATC_A
Cuts at: 1752 1752
Size: 4232
BfrBI ATG'CAT
Cuts at: 978 978
Size: 4232
BglI GCCn_nnn'nGGC
Cuts at: 881 881
Size: 4232
BglII A'GATC_T
Cuts at: 1747 3784 1747
Size: 2037 2195
BlpI GC'TnA_GC
Cuts at: 1344 1344
Size: 4232
Bme1580I G_kGCm'C
Cuts at: 1022 1857 1950 3006 1022
Size: 835 93 1056 2248
Fragments arranged by size:
2248 1056 835 93
BmrI ACTGGGnnnn_n'
Cuts at: 521 960 1017 1444 1525 1860 4004 521
Size: 439 57 427 81 335 2144 749
Fragments arranged by size:
2144 749 439 427 335 81 57
BplI GAGnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 1145 2599 1145
Size: 1454 2778
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 355 1408 2287 2779 3077 355
Size: 1053 879 492 298 1510
Fragments arranged by size:
1510 1053 879 492 298
BsaI GGTCTCn'nnnn_
Cuts at: 3936 3936
Size: 4232
BsaAI yAC'GTr
Cuts at: 1585 1585
Size: 4232
BsaBI GATnn'nnATC
Cuts at: 6 1746 3783 6
Size: 1740 2037 455
Fragments arranged by size:
2037 1740 455
BsaHI Gr'CG_yC
Cuts at: 286 1663 1911 2613 4109 286
Size: 1377 248 702 1496 409
Fragments arranged by size:
1496 1377 702 409 248
BsaWI w'CCGG_w
Cuts at: 1421 1942 2620 2894 3041 3452 1421
Size: 521 678 274 147 411 2201
Fragments arranged by size:
2201 678 521 411 274 147
BsaXI ACnnnnnCTCCnnnnnnn_nnn'
Cuts at: 1188 1218 1188
Size: 30 4202
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 278 505 1231 278
Size: 227 726 3279
Fragments arranged by size:
3279 726 227
BseYI C'CCAG_C
Cuts at: 1087 1186 1657 2415 2992 3488 1087
Size: 99 471 758 577 496 1831
Fragments arranged by size:
1831 758 577 496 471 99
BsgI GTGCAGnnnnnnnnnnnnnn_nn'
Cuts at: 612 612
Size: 4232
BsiEI CG_ry'CG
Cuts at: 104 536 1774 1820 3028 104
Size: 432 1238 46 1208 1308
Fragments arranged by size:
1308 1238 1208 432 46
BsiHKAI G_wGCw'C
Cuts at: 964 1150 1246 2024 2214 3006 964
Size: 186 96 778 190 792 2190
Fragments arranged by size:
2190 792 778 190 186 96
BsmI GAATG_Cn'
Cuts at: 124 978 124
Size: 854 3378
BsmBI CGTCTCn'nnnn_
Cuts at: 761 761
Size: 4232
Bsp1286I G_dGCh'C
Cuts at: 964 1022 1150 1187 1246 1857 1950 2024 2214
Size: 58 128 37 59 611 93 74 190
Cuts at: 2214 2276 3006 964
Size: 62 730 2190
Fragments arranged by size:
2190 730 611 190 128 93 74 62
59 58 37
BspEI T'CCGG_A
Cuts at: 3452 3452
Size: 4232
BspHI T'CATG_A
Cuts at: 581 3408 581
Size: 2827 1405
BspMI ACCTGCnnnn'nnnn_
Cuts at: 450 1282 1496 1798 2179 450
Size: 832 214 302 381 2503
Fragments arranged by size:
2503 832 381 302 214
BsrBI CCG'CTC
Cuts at: 50 369 2524 2578 4062 4227 50
Size: 319 2155 54 1484 165 55
Fragments arranged by size:
2155 1484 319 165 55 54
BsrDI GCAATG_nn'
Cuts at: 962 2144 962
Size: 1182 3050
BsrFI r'CCGG_y
Cuts at: 615 2230 2411 2620 615
Size: 1615 181 209 2227
Fragments arranged by size:
2227 1615 209 181
BssSI C'ACGA_G
Cuts at: 2503 2861 2503
Size: 358 3874
BstAPI GCAn_nnn'nTGC
Cuts at: 419 1609 419
Size: 1190 3042
BstBI TT'CG_AA
Cuts at: 2593 2593
Size: 4232
BstXI CCAn_nnnn'nTGG
Cuts at: 810 810
Size: 4232
BstYI r'GATC_y
Cuts at: 1 856 1257 1724 1747 2082 2328 3340 3426
Size: 855 401 467 23 335 246 1012 86
Cuts at: 3426 3438 3784 1
Size: 12 346 449
Fragments arranged by size:
1012 855 467 449 401 346 335 246
86 23 12
BtgI C'CryG_G
Cuts at: 319 351 803 319
Size: 32 452 3748
Fragments arranged by size:
3748 452 32
BtgZI GCGATGnnnnnnnnnn'nnnn_
Cuts at: 2182 2363 2526 2182
Size: 181 163 3888
Fragments arranged by size:
3888 181 163
BtsI GCAGTG_nn'
Cuts at: 520 600 632 1163 1475 520
Size: 80 32 531 312 3277
Fragments arranged by size:
3277 531 312 80 32
DraI TTT'AAA
Cuts at: 1231 1231
Size: 4232
DrdI GACnn_nn'nnGTC
Cuts at: 1938 2796 1938
Size: 858 3374
EaeI y'GGCC_r
Cuts at: 230 452 533 617 1817 1991 2382 2409 3754
Size: 222 81 84 1200 174 391 27 1345
Cuts at: 3754 230
Size: 708
Fragments arranged by size:
1345 1200 708 391 222 174 84 81
27
EagI C'GGCC_G
Cuts at: 533 1817 533
Size: 1284 2948
EarI CTCTTCn'nnn_
Cuts at: 2255 2465 2255
Size: 210 4022
EciI GGCGGAnnnnnnnnn_nn'
Cuts at: 2440 2750 2896 4042 2440
Size: 310 146 1146 2630
Fragments arranged by size:
2630 1146 310 146
EcoICRI GAG'CTC
Cuts at: 962 1244 962
Size: 282 3950
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 626 2057 2489 3236 3504 626
Size: 1431 432 747 268 1354
Fragments arranged by size:
1431 1354 747 432 268
EcoO109I rG'GnC_Cy
Cuts at: 882 1371 882
Size: 489 3743
EcoRI G'AATT_C
Cuts at: 1236 1236
Size: 4232
FalI AAGnnnnnCTTnnnnnnnn_nnnnn'
Cuts at: 2251 2251
Size: 4232
FspI TGC'GCA
Cuts at: 336 2013 336
Size: 1677 2555
FspAI rTGC'GCAy
Cuts at: 336 336
Size: 4232
HaeII r_GCGC'y
Cuts at: 220 248 387 1666 1914 2936 3915 220
Size: 28 139 1279 248 1022 979 537
Fragments arranged by size:
1279 1022 979 537 248 139 28
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 641 673 1033 1065 1162 1194 2074 2104 2106
Size: 32 360 32 97 32 880 30 2
Cuts at: 2106 2136 641
Size: 30 2737
Fragments arranged by size:
2737 880 360 97 32 32 32 30
30 2
HincII GTy'rAC
Cuts at: 1271 1271
Size: 4232
KasI G'GCGC_C
Cuts at: 1662 1910 1662
Size: 248 3984
KpnI G_GTAC'C
Cuts at: 175 1252 175
Size: 1077 3155
MluI A'CGCG_T
Cuts at: 3665 3665
Size: 4232
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 128 269 1160 2903 3087 128
Size: 141 891 1743 184 1273
Fragments arranged by size:
1743 1273 891 184 141
MscI TGG'CCA
Cuts at: 1993 1993
Size: 4232
MslI CAynn'nnrTG
Cuts at: 2221 2348 2221
Size: 127 4105
MspA1I CmG'CkG
Cuts at: 236 321 1343 1657 2017 3030 3275 236
Size: 85 1022 314 360 1013 245 1193
Fragments arranged by size:
1193 1022 1013 360 314 245 85
NaeI GCC'GGC
Cuts at: 617 2413 617
Size: 1796 2436
NarI GG'CG_CC
Cuts at: 1663 1911 1663
Size: 248 3984
NcoI C'CATG_G
Cuts at: 803 803
Size: 4232
NgoMIV G'CCGG_C
Cuts at: 615 2411 615
Size: 1796 2436
NsiI A_TGCA'T
Cuts at: 980 980
Size: 4232
NspI r_CATG'y
Cuts at: 396 697 1285 1785 2692 396
Size: 301 588 500 907 1936
Fragments arranged by size:
1936 907 588 500 301
PciI A'CATG_T
Cuts at: 2688 2688
Size: 4232
PfoI T'CCnGG_A
Cuts at: 662 662
Size: 4232
PmeI GTTT'AAAC
Cuts at: 1231 1231
Size: 4232
PpiI GAACnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 3371 3403 3371
Size: 32 4200
PspXI vC'TCGA_Gb
Cuts at: 240 240
Size: 4232
PsrI GAACnnnnnnTACnnnnnnn_nnnnn'
Cuts at: 340 372 340
Size: 32 4200
PstI C_TGCA'G
Cuts at: 346 639 1279 1964 346
Size: 293 640 685 2614
Fragments arranged by size:
2614 685 640 293
PvuI CG_AT'CG
Cuts at: 104 104
Size: 4232
PvuII CAG'CTG
Cuts at: 1657 2017 1657
Size: 360 3872
RsrII CG'GwC_CG
Cuts at: 2427 2427
Size: 4232
SacI G_AGCT'C
Cuts at: 964 1246 964
Size: 282 3950
SacII CC_GC'GG
Cuts at: 322 322
Size: 4232
SalI G'TCGA_C
Cuts at: 1269 1269
Size: 4232
SapI GCTCTTCn'nnn_
Cuts at: 2255 2465 2255
Size: 210 4022
SbfI CC_TGCA'GG
Cuts at: 1279 1279
Size: 4232
ScaI AGT'ACT
Cuts at: 799 3749 799
Size: 2950 1282
SexAI A'CCwGG_T
Cuts at: 713 713
Size: 4232
SfcI C'TryA_G
Cuts at: 33 342 635 735 1275 1960 2953 3144 33
Size: 309 293 100 540 685 993 191 1121
Fragments arranged by size:
1121 993 685 540 309 293 191 100
SfoI GGC'GCC
Cuts at: 1664 1912 1664
Size: 248 3984
SgfI GCG_AT'CGC
Cuts at: 104 104
Size: 4232
SmaI CCC'GGG
Cuts at: 168 1254 168
Size: 1086 3146
SmlI C'TyrA_G
Cuts at: 240 370 1119 1387 2302 2794 3056 240
Size: 130 749 268 915 492 262 1416
Fragments arranged by size:
1416 915 749 492 268 262 130
SpeI A'CTAG_T
Cuts at: 67 67
Size: 4232
SphI G_CATG'C
Cuts at: 1285 1785 1285
Size: 500 3732
StyI C'CwwG_G
Cuts at: 803 809 1366 1720 803
Size: 6 557 354 3315
Fragments arranged by size:
3315 557 354 6
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 286 1815 1948 2407 2616 286
Size: 1529 133 459 209 1902
Fragments arranged by size:
1902 1529 459 209 133
TatI w'GTAC_w
Cuts at: 797 968 3747 797
Size: 171 2779 1282
Fragments arranged by size:
2779 1282 171
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 848 880 884 916 1188 1220 3749 3781 848
Size: 32 4 32 272 32 2529 32 1299
Fragments arranged by size:
2529 1299 272 32 32 32 32 4
Tth111I GACn'n_nGTC
Cuts at: 2029 2029
Size: 4232
XbaI T'CTAG_A
Cuts at: 1263 1263
Size: 4232
XcmI CCAnnnn_n'nnnnTGG
Cuts at: 1727 1727
Size: 4232
XhoI C'TCGA_G
Cuts at: 240 1119 240
Size: 879 3353
XmaI C'CCGG_G
Cuts at: 166 1252 166
Size: 1086 3146
Enzymes that do cut:
AarI AccI Acc65I AclI AcuI AfeI AflIII AgeI
AhdI AlwNI ApaLI ApoI AseI AsiSI AvaI BamHI
BanI BanII BbeI BcgI BciVI BclI BfrBI BglI
BglII BlpI Bme1580I BmrI BplI BpuEI BsaI BsaAI
BsaBI BsaHI BsaWI BsaXI BseRI BseYI BsgI BsiEI
BsiHKAI BsmI BsmBI Bsp1286I BspEI BspHI BspMI BsrBI
BsrDI BsrFI BssSI BstAPI BstBI BstXI BstYI BtgI
BtgZI BtsI DraI DrdI EaeI EagI EarI EciI
EcoICRI Eco57MI EcoO109I EcoRI FalI FspI FspAI HaeII
Hin4I HincII KasI KpnI MluI MmeI MscI MslI
MspA1I NaeI NarI NcoI NgoMIV NsiI NspI PciI
PfoI PmeI PpiI PspXI PsrI PstI PvuI PvuII
RsrII SacI SacII SalI SapI SbfI ScaI SexAI
SfcI SfoI SgfI SmaI SmlI SpeI SphI StyI
TaqII TatI TstI Tth111I XbaI XcmI XhoI XmaI
Enzymes that do not cut:
AatII AflII AleI AlfI AloI ApaI AscI AvrII
BaeI BbsI BbvCI BmgBI BmtI BpmI Bpu10I BsiWI
BsrGI BssHII BstEII BstZ17I Bsu36I ClaI CspCI DraIII
EcoNI EcoRV FseI HindIII HpaI MfeI NdeI NheI
NotI NruI PacI PasI PflMI PmlI PpuMI PshAI
PsiI PspOMI SanDI SfiI SgrAI SnaBI SrfI SspI
StuI SwaI XmnI ZraI