Restriction map for
pF1KB7623
(Circular) (Six-base) MAPSORT of: pF1KB7623.seq Check: 2019 from: 1 to: 4055
pF1KB7623 4055 bp
With 182 enzymes: SgfI *
June 20, 2008 10:27 ..
AccI GT'mk_AC
Cuts at: 926 1093 926
Size: 167 3888
Acc65I G'GTAC_C
Cuts at: 1071 1071
Size: 4055
AclI AA'CG_TT
Cuts at: 3895 3895
Size: 4055
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 1880 2312 3059 3327 1880
Size: 432 747 268 2608
Fragments arranged by size:
2608 747 432 268
AflIII A'CryG_T
Cuts at: 626 935 1031 1407 2511 3488 626
Size: 309 96 376 1104 977 1193
Fragments arranged by size:
1193 1104 977 376 309 96
AgeI A'CCGG_T
Cuts at: 2443 2443
Size: 4055
AlwNI CAG_nnn'CTG
Cuts at: 564 1360 2927 564
Size: 796 1567 1692
Fragments arranged by size:
1692 1567 796
ApaI G_GGCC'C
Cuts at: 194 308 799 1015 194
Size: 114 491 216 3234
Fragments arranged by size:
3234 491 216 114
ApaLI G'TGCA_C
Cuts at: 2825 2825
Size: 4055
ApoI r'AATT_y
Cuts at: 1059 3408 3664 1059
Size: 2349 256 1450
Fragments arranged by size:
2349 1450 256
AseI AT'TA_AT
Cuts at: 20 20
Size: 4055
AsiSI GCG_AT'CGC
Cuts at: 104 104
Size: 4055
AvaI C'yCGr_G
Cuts at: 235 307 825 945 1020 1075 235
Size: 72 518 120 75 55 3215
Fragments arranged by size:
3215 518 120 75 72 55
BamHI G'GATC_C
Cuts at: 1080 1080
Size: 4055
BanI G'GyrC_C
Cuts at: 675 802 1071 1485 1733 1768 675
Size: 127 269 414 248 35 2962
Fragments arranged by size:
2962 414 269 248 127 35
BanII G_rGCy'C
Cuts at: 158 194 308 727 799 1015 1069 2099 158
Size: 36 114 419 72 216 54 1030 2114
Fragments arranged by size:
2114 1030 419 216 114 72 54 36
BbeI G_GCGC'C
Cuts at: 679 806 1489 1737 679
Size: 127 683 248 2997
Fragments arranged by size:
2997 683 248 127
BciVI GTATCCnnnnn_n'
Cuts at: 1953 2714 4055 1953
Size: 761 1341 1953
Fragments arranged by size:
1953 1341 761
BclI T'GATC_A
Cuts at: 1575 1575
Size: 4055
BglI GCCn_nnn'nGGC
Cuts at: 153 899 1010 153
Size: 746 111 3198
Fragments arranged by size:
3198 746 111
BglII A'GATC_T
Cuts at: 1570 3607 1570
Size: 2037 2018
BlpI GC'TnA_GC
Cuts at: 1167 1167
Size: 4055
Bme1580I G_kGCm'C
Cuts at: 194 308 799 1015 1680 1773 2829 194
Size: 114 491 216 665 93 1056 1420
Fragments arranged by size:
1420 1056 665 491 216 114 93
BmgBI CAC'GTC
Cuts at: 627 627
Size: 4055
BmrI ACTGGGnnnn_n'
Cuts at: 666 1267 1348 1683 3827 666
Size: 601 81 335 2144 894
Fragments arranged by size:
2144 894 601 335 81
BplI GAGnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 2422 2422
Size: 4055
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 157 157
Size: 4055
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 952 1231 2110 2602 2900 952
Size: 279 879 492 298 2107
Fragments arranged by size:
2107 879 492 298 279
BsaI GGTCTCn'nnnn_
Cuts at: 3759 3759
Size: 4055
BsaAI yAC'GTr
Cuts at: 588 938 1408 588
Size: 350 470 3235
Fragments arranged by size:
3235 470 350
BsaBI GATnn'nnATC
Cuts at: 6 1569 3606 6
Size: 1563 2037 455
Fragments arranged by size:
2037 1563 455
BsaHI Gr'CG_yC
Cuts at: 113 676 803 1486 1734 2436 3932 113
Size: 563 127 683 248 702 1496 236
Fragments arranged by size:
1496 702 683 563 248 236 127
BsaWI w'CCGG_w
Cuts at: 1244 1765 2443 2717 2864 3275 1244
Size: 521 678 274 147 411 2024
Fragments arranged by size:
2024 678 521 411 274 147
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 481 759 849 481
Size: 278 90 3687
Fragments arranged by size:
3687 278 90
BseYI C'CCAG_C
Cuts at: 354 757 1480 2238 2815 3311 354
Size: 403 723 758 577 496 1098
Fragments arranged by size:
1098 758 723 577 496 403
BsgI GTGCAGnnnnnnnnnnnnnn_nn'
Cuts at: 631 631
Size: 4055
BsiEI CG_ry'CG
Cuts at: 104 163 1597 1643 2851 104
Size: 59 1434 46 1208 1308
Fragments arranged by size:
1434 1308 1208 59 46
BsiHKAI G_wGCw'C
Cuts at: 1069 1847 2037 2829 1069
Size: 778 190 792 2295
Fragments arranged by size:
2295 792 778 190
BsiWI C'GTAC_G
Cuts at: 385 896 385
Size: 511 3544
Bsp1286I G_dGCh'C
Cuts at: 158 194 308 727 799 1015 1069 1680 1773
Size: 36 114 419 72 216 54 611 93
Cuts at: 1773 1847 2037 2099 2829 158
Size: 74 190 62 730 1384
Fragments arranged by size:
1384 730 611 419 216 190 114 93
74 72 62 54 36
BspEI T'CCGG_A
Cuts at: 3275 3275
Size: 4055
BspHI T'CATG_A
Cuts at: 3231 3231
Size: 4055
BspMI ACCTGCnnnn'nnnn_
Cuts at: 1105 1319 1621 2002 1105
Size: 214 302 381 3158
Fragments arranged by size:
3158 381 302 214
BsrBI CCG'CTC
Cuts at: 50 141 234 687 2347 2401 3885 4050 50
Size: 91 93 453 1660 54 1484 165 55
Fragments arranged by size:
1660 1484 453 165 93 91 55 54
BsrDI GCAATG_nn'
Cuts at: 1967 1967
Size: 4055
BsrFI r'CCGG_y
Cuts at: 637 853 2053 2234 2443 637
Size: 216 1200 181 209 2249
Fragments arranged by size:
2249 1200 216 209 181
BssSI C'ACGA_G
Cuts at: 2326 2684 2326
Size: 358 3697
BstAPI GCAn_nnn'nTGC
Cuts at: 335 1432 335
Size: 1097 2958
BstBI TT'CG_AA
Cuts at: 2416 2416
Size: 4055
BstXI CCAn_nnnn'nTGG
Cuts at: 672 672
Size: 4055
BstYI r'GATC_y
Cuts at: 1 1080 1547 1570 1905 2151 3163 3249 3261
Size: 1079 467 23 335 246 1012 86 12
Cuts at: 3261 3607 1
Size: 346 449
Fragments arranged by size:
1079 1012 467 449 346 335 246 86
23 12
BtgI C'CryG_G
Cuts at: 107 107
Size: 4055
BtgZI GCGATGnnnnnnnnnn'nnnn_
Cuts at: 2005 2186 2349 2005
Size: 181 163 3711
Fragments arranged by size:
3711 181 163
BtsI GCAGTG_nn'
Cuts at: 1298 1298
Size: 4055
DraI TTT'AAA
Cuts at: 1054 1054
Size: 4055
DrdI GACnn_nn'nnGTC
Cuts at: 783 1761 2619 783
Size: 978 858 2219
Fragments arranged by size:
2219 978 858
EaeI y'GGCC_r
Cuts at: 160 872 1640 1814 2205 2232 3577 160
Size: 712 768 174 391 27 1345 638
Fragments arranged by size:
1345 768 712 638 391 174 27
EagI C'GGCC_G
Cuts at: 160 1640 160
Size: 1480 2575
EarI CTCTTCn'nnn_
Cuts at: 2078 2288 2078
Size: 210 3845
EciI GGCGGAnnnnnnnnn_nn'
Cuts at: 234 2263 2573 2719 3865 234
Size: 2029 310 146 1146 424
Fragments arranged by size:
2029 1146 424 310 146
EcoICRI GAG'CTC
Cuts at: 1067 1067
Size: 4055
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 157 1880 2312 3059 3327 157
Size: 1723 432 747 268 885
Fragments arranged by size:
1723 885 747 432 268
EcoNI CCTnn'n_nnAGG
Cuts at: 574 574
Size: 4055
EcoO109I rG'GnC_Cy
Cuts at: 190 304 1011 1194 190
Size: 114 707 183 3051
Fragments arranged by size:
3051 707 183 114
EcoRI G'AATT_C
Cuts at: 1059 1059
Size: 4055
FalI AAGnnnnnCTTnnnnnnnn_nnnnn'
Cuts at: 2074 2074
Size: 4055
FspI TGC'GCA
Cuts at: 1836 1836
Size: 4055
HaeII r_GCGC'y
Cuts at: 679 685 806 890 1489 1737 2759 3738 679
Size: 6 121 84 599 248 1022 979 996
Fragments arranged by size:
1022 996 979 599 248 121 84 6
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 1897 1927 1929 1959 1897
Size: 30 2 30 3993
Fragments arranged by size:
3993 30 30 2
HincII GTy'rAC
Cuts at: 1094 1094
Size: 4055
KasI G'GCGC_C
Cuts at: 675 802 1485 1733 675
Size: 127 683 248 2997
Fragments arranged by size:
2997 683 248 127
KpnI G_GTAC'C
Cuts at: 1075 1075
Size: 4055
MluI A'CGCG_T
Cuts at: 1031 3488 1031
Size: 2457 1598
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 2726 2910 2726
Size: 184 3871
MscI TGG'CCA
Cuts at: 1816 1816
Size: 4055
MslI CAynn'nnrTG
Cuts at: 457 778 2044 2171 457
Size: 321 1266 127 2341
Fragments arranged by size:
2341 1266 321 127
MspA1I CmG'CkG
Cuts at: 375 564 602 729 1166 1480 1840 2853 3098
Size: 189 38 127 437 314 360 1013 245
Cuts at: 3098 375
Size: 1332
Fragments arranged by size:
1332 1013 437 360 314 245 189 127
38
NaeI GCC'GGC
Cuts at: 639 2236 639
Size: 1597 2458
NarI GG'CG_CC
Cuts at: 676 803 1486 1734 676
Size: 127 683 248 2997
Fragments arranged by size:
2997 683 248 127
NcoI C'CATG_G
Cuts at: 107 107
Size: 4055
NgoMIV G'CCGG_C
Cuts at: 637 2234 637
Size: 1597 2458
NspI r_CATG'y
Cuts at: 819 1108 1608 2515 819
Size: 289 500 907 2359
Fragments arranged by size:
2359 907 500 289
PciI A'CATG_T
Cuts at: 2511 2511
Size: 4055
PflMI CCAn_nnn'nTGG
Cuts at: 186 564 186
Size: 378 3677
PmeI GTTT'AAAC
Cuts at: 1054 1054
Size: 4055
PmlI CAC'GTG
Cuts at: 938 938
Size: 4055
PpiI GAACnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 412 444 3194 3226 412
Size: 32 2750 32 1241
Fragments arranged by size:
2750 1241 32 32
PspOMI G'GGCC_C
Cuts at: 190 304 795 1011 190
Size: 114 491 216 3234
Fragments arranged by size:
3234 491 216 114
PspXI vC'TCGA_Gb
Cuts at: 945 945
Size: 4055
PstI C_TGCA'G
Cuts at: 1010 1102 1787 1010
Size: 92 685 3278
Fragments arranged by size:
3278 685 92
PvuI CG_AT'CG
Cuts at: 104 104
Size: 4055
PvuII CAG'CTG
Cuts at: 564 1480 1840 564
Size: 916 360 2779
Fragments arranged by size:
2779 916 360
RsrII CG'GwC_CG
Cuts at: 2250 2250
Size: 4055
SacI G_AGCT'C
Cuts at: 1069 1069
Size: 4055
SalI G'TCGA_C
Cuts at: 1092 1092
Size: 4055
SapI GCTCTTCn'nnn_
Cuts at: 2078 2288 2078
Size: 210 3845
SbfI CC_TGCA'GG
Cuts at: 1010 1102 1010
Size: 92 3963
ScaI AGT'ACT
Cuts at: 3572 3572
Size: 4055
SfcI C'TryA_G
Cuts at: 33 918 1006 1098 1783 2776 2967 33
Size: 885 88 92 685 993 191 1121
Fragments arranged by size:
1121 993 885 685 191 92 88
SfiI GGCCn_nnn'nGGCC
Cuts at: 1010 1010
Size: 4055
SfoI GGC'GCC
Cuts at: 677 804 1487 1735 677
Size: 127 683 248 2997
Fragments arranged by size:
2997 683 248 127
SgfI GCG_AT'CGC
Cuts at: 104 104
Size: 4055
SmaI CCC'GGG
Cuts at: 1077 1077
Size: 4055
SmlI C'TyrA_G
Cuts at: 235 945 967 1210 2125 2617 2879 235
Size: 710 22 243 915 492 262 1411
Fragments arranged by size:
1411 915 710 492 262 243 22
SpeI A'CTAG_T
Cuts at: 67 67
Size: 4055
SphI G_CATG'C
Cuts at: 819 1108 1608 819
Size: 289 500 3266
Fragments arranged by size:
3266 500 289
StyI C'CwwG_G
Cuts at: 107 299 1189 1543 107
Size: 192 890 354 2619
Fragments arranged by size:
2619 890 354 192
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 771 1638 1771 2230 2439 771
Size: 867 133 459 209 2387
Fragments arranged by size:
2387 867 459 209 133
TatI w'GTAC_w
Cuts at: 1040 3570 1040
Size: 2530 1525
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 3572 3604 3572
Size: 32 4023
Tth111I GACn'n_nGTC
Cuts at: 1852 1852
Size: 4055
XbaI T'CTAG_A
Cuts at: 1086 1086
Size: 4055
XcmI CCAnnnn_n'nnnnTGG
Cuts at: 1550 1550
Size: 4055
XhoI C'TCGA_G
Cuts at: 235 945 235
Size: 710 3345
XmaI C'CCGG_G
Cuts at: 1075 1075
Size: 4055
Enzymes that do cut:
AccI Acc65I AclI AcuI AflIII AgeI AlwNI ApaI
ApaLI ApoI AseI AsiSI AvaI BamHI BanI BanII
BbeI BciVI BclI BglI BglII BlpI Bme1580I BmgBI
BmrI BplI BpmI BpuEI BsaI BsaAI BsaBI BsaHI
BsaWI BseRI BseYI BsgI BsiEI BsiHKAI BsiWI Bsp1286I
BspEI BspHI BspMI BsrBI BsrDI BsrFI BssSI BstAPI
BstBI BstXI BstYI BtgI BtgZI BtsI DraI DrdI
EaeI EagI EarI EciI EcoICRI Eco57MI EcoNI EcoO109I
EcoRI FalI FspI HaeII Hin4I HincII KasI KpnI
MluI MmeI MscI MslI MspA1I NaeI NarI NcoI
NgoMIV NspI PciI PflMI PmeI PmlI PpiI PspOMI
PspXI PstI PvuI PvuII RsrII SacI SalI SapI
SbfI ScaI SfcI SfiI SfoI SgfI SmaI SmlI
SpeI SphI StyI TaqII TatI TstI Tth111I XbaI
XcmI XhoI XmaI
Enzymes that do not cut:
AarI AatII AfeI AflII AhdI AleI AlfI AloI
AscI AvrII BaeI BbsI BbvCI BcgI BfrBI BmtI
Bpu10I BsaXI BsmI BsmBI BsrGI BssHII BstEII BstZ17I
Bsu36I ClaI CspCI DraIII EcoRV FseI FspAI HindIII
HpaI MfeI NdeI NheI NotI NruI NsiI PacI
PasI PfoI PpuMI PshAI PsiI PsrI SacII SanDI
SexAI SgrAI SnaBI SrfI SspI StuI SwaI XmnI
ZraI