Restriction map for
pF1KB7617
(Circular) (Six-base) MAPSORT of: pF1KB7617.seq Check: 7143 from: 1 to: 4034
pF1KB7617 4034 bp
With 182 enzymes: SgfI *
October 7, 2009 14:23 ..
AccI GT'mk_AC
Cuts at: 1072 1072
Size: 4034
Acc65I G'GTAC_C
Cuts at: 1050 1050
Size: 4034
AclI AA'CG_TT
Cuts at: 3874 3874
Size: 4034
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 1859 2291 3038 3306 1859
Size: 432 747 268 2587
Fragments arranged by size:
2587 747 432 268
AflII C'TTAA_G
Cuts at: 142 142
Size: 4034
AflIII A'CryG_T
Cuts at: 1386 2490 3467 1386
Size: 1104 977 1953
Fragments arranged by size:
1953 1104 977
AgeI A'CCGG_T
Cuts at: 2422 2422
Size: 4034
AhdI GACnn_n'nnGTC
Cuts at: 702 702
Size: 4034
AleI CACnn'nnGTG
Cuts at: 727 727
Size: 4034
AlwNI CAG_nnn'CTG
Cuts at: 1339 2906 1339
Size: 1567 2467
ApaLI G'TGCA_C
Cuts at: 2804 2804
Size: 4034
ApoI r'AATT_y
Cuts at: 137 163 277 316 534 596 1038 3387 3643
Size: 26 114 39 218 62 442 2349 256
Cuts at: 3643 137
Size: 528
Fragments arranged by size:
2349 528 442 256 218 114 62 39
26
AseI AT'TA_AT
Cuts at: 20 20
Size: 4034
AsiSI GCG_AT'CGC
Cuts at: 104 104
Size: 4034
AvaI C'yCGr_G
Cuts at: 1054 1054
Size: 4034
BamHI G'GATC_C
Cuts at: 1059 1059
Size: 4034
BanI G'GyrC_C
Cuts at: 1050 1464 1712 1747 1050
Size: 414 248 35 3337
Fragments arranged by size:
3337 414 248 35
BanII G_rGCy'C
Cuts at: 388 1048 2078 388
Size: 660 1030 2344
Fragments arranged by size:
2344 1030 660
BbeI G_GCGC'C
Cuts at: 1468 1716 1468
Size: 248 3786
BciVI GTATCCnnnnn_n'
Cuts at: 1932 2693 4034 1932
Size: 761 1341 1932
Fragments arranged by size:
1932 1341 761
BclI T'GATC_A
Cuts at: 1554 1554
Size: 4034
BglII A'GATC_T
Cuts at: 1549 3586 1549
Size: 2037 1997
BlpI GC'TnA_GC
Cuts at: 643 1146 643
Size: 503 3531
Bme1580I G_kGCm'C
Cuts at: 1659 1752 2808 1659
Size: 93 1056 2885
Fragments arranged by size:
2885 1056 93
BmrI ACTGGGnnnn_n'
Cuts at: 1246 1327 1662 3806 1246
Size: 81 335 2144 1474
Fragments arranged by size:
2144 1474 335 81
BplI GAGnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 2401 2401
Size: 4034
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 526 526
Size: 4034
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 1210 2089 2581 2879 1210
Size: 879 492 298 2365
Fragments arranged by size:
2365 879 492 298
BsaI GGTCTCn'nnnn_
Cuts at: 3738 3738
Size: 4034
BsaAI yAC'GTr
Cuts at: 1387 1387
Size: 4034
BsaBI GATnn'nnATC
Cuts at: 6 500 1548 3585 6
Size: 494 1048 2037 455
Fragments arranged by size:
2037 1048 494 455
BsaHI Gr'CG_yC
Cuts at: 1465 1713 2415 3911 1465
Size: 248 702 1496 1588
Fragments arranged by size:
1588 1496 702 248
BsaWI w'CCGG_w
Cuts at: 1223 1744 2422 2696 2843 3254 1223
Size: 521 678 274 147 411 2003
Fragments arranged by size:
2003 678 521 411 274 147
BsaXI ACnnnnnCTCCnnnnnnn_nnn'
Cuts at: 798 828 798
Size: 30 4004
BseYI C'CCAG_C
Cuts at: 1459 2217 2794 3290 1459
Size: 758 577 496 2203
Fragments arranged by size:
2203 758 577 496
BsgI GTGCAGnnnnnnnnnnnnnn_nn'
Cuts at: 734 734
Size: 4034
BsiEI CG_ry'CG
Cuts at: 104 1576 1622 2830 104
Size: 1472 46 1208 1308
Fragments arranged by size:
1472 1308 1208 46
BsiHKAI G_wGCw'C
Cuts at: 388 1048 1826 2016 2808 388
Size: 660 778 190 792 1614
Fragments arranged by size:
1614 792 778 660 190
BsmI GAATG_Cn'
Cuts at: 150 899 150
Size: 749 3285
Bsp1286I G_dGCh'C
Cuts at: 388 1048 1659 1752 1826 2016 2078 2808 388
Size: 660 611 93 74 190 62 730 1614
Fragments arranged by size:
1614 730 660 611 190 93 74 62
BspEI T'CCGG_A
Cuts at: 3254 3254
Size: 4034
BspHI T'CATG_A
Cuts at: 3210 3210
Size: 4034
BspMI ACCTGCnnnn'nnnn_
Cuts at: 802 1084 1298 1600 1981 802
Size: 282 214 302 381 2855
Fragments arranged by size:
2855 381 302 282 214
BsrBI CCG'CTC
Cuts at: 50 2326 2380 3864 4029 50
Size: 2276 54 1484 165 55
Fragments arranged by size:
2276 1484 165 55 54
BsrDI GCAATG_nn'
Cuts at: 607 1946 607
Size: 1339 2695
BsrFI r'CCGG_y
Cuts at: 2032 2213 2422 2032
Size: 181 209 3644
Fragments arranged by size:
3644 209 181
BsrGI T'GTAC_A
Cuts at: 483 483
Size: 4034
BssSI C'ACGA_G
Cuts at: 2305 2663 2305
Size: 358 3676
BstAPI GCAn_nnn'nTGC
Cuts at: 1411 1411
Size: 4034
BstBI TT'CG_AA
Cuts at: 2395 2395
Size: 4034
BstYI r'GATC_y
Cuts at: 1 236 1059 1526 1549 1884 2130 3142 3228
Size: 235 823 467 23 335 246 1012 86
Cuts at: 3228 3240 3586 1
Size: 12 346 449
Fragments arranged by size:
1012 823 467 449 346 335 246 235
86 23 12
BtgI C'CryG_G
Cuts at: 107 107
Size: 4034
BtgZI GCGATGnnnnnnnnnn'nnnn_
Cuts at: 1984 2165 2328 1984
Size: 181 163 3690
Fragments arranged by size:
3690 181 163
BtsI GCAGTG_nn'
Cuts at: 1277 1277
Size: 4034
DraI TTT'AAA
Cuts at: 1033 1033
Size: 4034
DrdI GACnn_nn'nnGTC
Cuts at: 1740 2598 1740
Size: 858 3176
EaeI y'GGCC_r
Cuts at: 515 1619 1793 2184 2211 3556 515
Size: 1104 174 391 27 1345 993
Fragments arranged by size:
1345 1104 993 391 174 27
EagI C'GGCC_G
Cuts at: 1619 1619
Size: 4034
EarI CTCTTCn'nnn_
Cuts at: 2057 2267 2057
Size: 210 3824
EciI GGCGGAnnnnnnnnn_nn'
Cuts at: 686 2242 2552 2698 3844 686
Size: 1556 310 146 1146 876
Fragments arranged by size:
1556 1146 876 310 146
EcoICRI GAG'CTC
Cuts at: 386 1046 386
Size: 660 3374
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 526 1859 2291 3038 3306 526
Size: 1333 432 747 268 1254
Fragments arranged by size:
1333 1254 747 432 268
EcoO109I rG'GnC_Cy
Cuts at: 1173 1173
Size: 4034
EcoRI G'AATT_C
Cuts at: 277 1038 277
Size: 761 3273
FalI AAGnnnnnCTTnnnnnnnn_nnnnn'
Cuts at: 2053 2053
Size: 4034
FspI TGC'GCA
Cuts at: 1815 1815
Size: 4034
HaeII r_GCGC'y
Cuts at: 1468 1716 2738 3717 1468
Size: 248 1022 979 1785
Fragments arranged by size:
1785 1022 979 248
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 462 494 688 720 1876 1906 1908 1938 462
Size: 32 194 32 1156 30 2 30 2558
Fragments arranged by size:
2558 1156 194 32 32 30 30 2
HincII GTy'rAC
Cuts at: 1003 1073 1003
Size: 70 3964
HindIII A'AGCT_T
Cuts at: 244 244
Size: 4034
KasI G'GCGC_C
Cuts at: 1464 1712 1464
Size: 248 3786
KpnI G_GTAC'C
Cuts at: 1054 1054
Size: 4034
MluI A'CGCG_T
Cuts at: 3467 3467
Size: 4034
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 2705 2889 2705
Size: 184 3850
MscI TGG'CCA
Cuts at: 1795 1795
Size: 4034
MslI CAynn'nnrTG
Cuts at: 727 904 2023 2150 727
Size: 177 1119 127 2611
Fragments arranged by size:
2611 1119 177 127
MspA1I CmG'CkG
Cuts at: 1145 1459 1819 2832 3077 1145
Size: 314 360 1013 245 2102
Fragments arranged by size:
2102 1013 360 314 245
NaeI GCC'GGC
Cuts at: 2215 2215
Size: 4034
NarI GG'CG_CC
Cuts at: 1465 1713 1465
Size: 248 3786
NcoI C'CATG_G
Cuts at: 107 107
Size: 4034
NgoMIV G'CCGG_C
Cuts at: 2213 2213
Size: 4034
NspI r_CATG'y
Cuts at: 1087 1587 2494 1087
Size: 500 907 2627
Fragments arranged by size:
2627 907 500
PacI TTA_AT'TAA
Cuts at: 935 935
Size: 4034
PciI A'CATG_T
Cuts at: 2490 2490
Size: 4034
PfoI T'CCnGG_A
Cuts at: 706 706
Size: 4034
PmeI GTTT'AAAC
Cuts at: 1033 1033
Size: 4034
PpiI GAACnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 3173 3205 3173
Size: 32 4002
PsiI TTA'TAA
Cuts at: 169 169
Size: 4034
PstI C_TGCA'G
Cuts at: 1081 1766 1081
Size: 685 3349
PvuI CG_AT'CG
Cuts at: 104 104
Size: 4034
PvuII CAG'CTG
Cuts at: 1459 1819 1459
Size: 360 3674
RsrII CG'GwC_CG
Cuts at: 2229 2229
Size: 4034
SacI G_AGCT'C
Cuts at: 388 1048 388
Size: 660 3374
SalI G'TCGA_C
Cuts at: 1071 1071
Size: 4034
SapI GCTCTTCn'nnn_
Cuts at: 2057 2267 2057
Size: 210 3824
SbfI CC_TGCA'GG
Cuts at: 1081 1081
Size: 4034
ScaI AGT'ACT
Cuts at: 3551 3551
Size: 4034
SfcI C'TryA_G
Cuts at: 33 1077 1762 2755 2946 33
Size: 1044 685 993 191 1121
Fragments arranged by size:
1121 1044 993 685 191
SfoI GGC'GCC
Cuts at: 1466 1714 1466
Size: 248 3786
SgfI GCG_AT'CGC
Cuts at: 104 104
Size: 4034
SmaI CCC'GGG
Cuts at: 1056 1056
Size: 4034
SmlI C'TyrA_G
Cuts at: 142 1189 2104 2596 2858 142
Size: 1047 915 492 262 1318
Fragments arranged by size:
1318 1047 915 492 262
SpeI A'CTAG_T
Cuts at: 67 67
Size: 4034
SphI G_CATG'C
Cuts at: 1087 1587 1087
Size: 500 3534
SspI AAT'ATT
Cuts at: 219 219
Size: 4034
StyI C'CwwG_G
Cuts at: 107 1168 1522 107
Size: 1061 354 2619
Fragments arranged by size:
2619 1061 354
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 1617 1750 2209 2418 1617
Size: 133 459 209 3233
Fragments arranged by size:
3233 459 209 133
TatI w'GTAC_w
Cuts at: 483 3549 483
Size: 3066 968
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 3551 3583 3551
Size: 32 4002
Tth111I GACn'n_nGTC
Cuts at: 1831 1831
Size: 4034
XbaI T'CTAG_A
Cuts at: 1065 1065
Size: 4034
XcmI CCAnnnn_n'nnnnTGG
Cuts at: 1529 1529
Size: 4034
XmaI C'CCGG_G
Cuts at: 1054 1054
Size: 4034
XmnI GAAnn'nnTTC
Cuts at: 899 899
Size: 4034
Enzymes that do cut:
AccI Acc65I AclI AcuI AflII AflIII AgeI AhdI
AleI AlwNI ApaLI ApoI AseI AsiSI AvaI BamHI
BanI BanII BbeI BciVI BclI BglII BlpI Bme1580I
BmrI BplI BpmI BpuEI BsaI BsaAI BsaBI BsaHI
BsaWI BsaXI BseYI BsgI BsiEI BsiHKAI BsmI Bsp1286I
BspEI BspHI BspMI BsrBI BsrDI BsrFI BsrGI BssSI
BstAPI BstBI BstYI BtgI BtgZI BtsI DraI DrdI
EaeI EagI EarI EciI EcoICRI Eco57MI EcoO109I EcoRI
FalI FspI HaeII Hin4I HincII HindIII KasI KpnI
MluI MmeI MscI MslI MspA1I NaeI NarI NcoI
NgoMIV NspI PacI PciI PfoI PmeI PpiI PsiI
PstI PvuI PvuII RsrII SacI SalI SapI SbfI
ScaI SfcI SfoI SgfI SmaI SmlI SpeI SphI
SspI StyI TaqII TatI TstI Tth111I XbaI XcmI
XmaI XmnI
Enzymes that do not cut:
AarI AatII AfeI AlfI AloI ApaI AscI AvrII
BaeI BbsI BbvCI BcgI BfrBI BglI BmgBI BmtI
Bpu10I BseRI BsiWI BsmBI BssHII BstEII BstXI BstZ17I
Bsu36I ClaI CspCI DraIII EcoNI EcoRV FseI FspAI
HpaI MfeI NdeI NheI NotI NruI NsiI PasI
PflMI PmlI PpuMI PshAI PspOMI PspXI PsrI SacII
SanDI SexAI SfiI SgrAI SnaBI SrfI StuI SwaI
XhoI ZraI