Restriction map for
pF1KB7605
(Circular) (Six-base) MAPSORT of: pF1KB7605.seq Check: 7852 from: 1 to: 4010
pF1KB7605 4010 bp
With 182 enzymes: SgfI *
June 20, 2008 10:27 ..
AccI GT'mk_AC
Cuts at: 1048 1048
Size: 4010
Acc65I G'GTAC_C
Cuts at: 1026 1026
Size: 4010
AclI AA'CG_TT
Cuts at: 3850 3850
Size: 4010
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 293 1019 1835 2267 3014 3282 293
Size: 726 816 432 747 268 1021
Fragments arranged by size:
1021 816 747 726 432 268
AflII C'TTAA_G
Cuts at: 394 729 394
Size: 335 3675
AflIII A'CryG_T
Cuts at: 1362 2466 3443 1362
Size: 1104 977 1929
Fragments arranged by size:
1929 1104 977
AgeI A'CCGG_T
Cuts at: 2398 2398
Size: 4010
AlwNI CAG_nnn'CTG
Cuts at: 1315 2882 1315
Size: 1567 2443
ApaLI G'TGCA_C
Cuts at: 2780 2780
Size: 4010
ApoI r'AATT_y
Cuts at: 454 566 898 1014 3363 3619 454
Size: 112 332 116 2349 256 845
Fragments arranged by size:
2349 845 332 256 116 112
AseI AT'TA_AT
Cuts at: 20 152 20
Size: 132 3878
AsiSI GCG_AT'CGC
Cuts at: 104 104
Size: 4010
AvaI C'yCGr_G
Cuts at: 1030 1030
Size: 4010
BamHI G'GATC_C
Cuts at: 1035 1035
Size: 4010
BanI G'GyrC_C
Cuts at: 1026 1440 1688 1723 1026
Size: 414 248 35 3313
Fragments arranged by size:
3313 414 248 35
BanII G_rGCy'C
Cuts at: 326 1024 2054 326
Size: 698 1030 2282
Fragments arranged by size:
2282 1030 698
BbeI G_GCGC'C
Cuts at: 1444 1692 1444
Size: 248 3762
BbsI GAAGACnn'nnnn_
Cuts at: 619 784 619
Size: 165 3845
BciVI GTATCCnnnnn_n'
Cuts at: 1908 2669 4010 1908
Size: 761 1341 1908
Fragments arranged by size:
1908 1341 761
BclI T'GATC_A
Cuts at: 690 1530 690
Size: 840 3170
BglII A'GATC_T
Cuts at: 966 1525 3562 966
Size: 559 2037 1414
Fragments arranged by size:
2037 1414 559
BlpI GC'TnA_GC
Cuts at: 1122 1122
Size: 4010
Bme1580I G_kGCm'C
Cuts at: 1635 1728 2784 1635
Size: 93 1056 2861
Fragments arranged by size:
2861 1056 93
BmrI ACTGGGnnnn_n'
Cuts at: 1222 1303 1638 3782 1222
Size: 81 335 2144 1450
Fragments arranged by size:
2144 1450 335 81
BplI GAGnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 2377 2377
Size: 4010
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 835 835
Size: 4010
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 207 208 1186 2065 2557 2855 207
Size: 1 978 879 492 298 1362
Fragments arranged by size:
1362 978 879 492 298 1
BsaI GGTCTCn'nnnn_
Cuts at: 3714 3714
Size: 4010
BsaAI yAC'GTr
Cuts at: 1363 1363
Size: 4010
BsaBI GATnn'nnATC
Cuts at: 6 1524 3561 6
Size: 1518 2037 455
Fragments arranged by size:
2037 1518 455
BsaHI Gr'CG_yC
Cuts at: 1441 1689 2391 3887 1441
Size: 248 702 1496 1564
Fragments arranged by size:
1564 1496 702 248
BsaWI w'CCGG_w
Cuts at: 1199 1720 2398 2672 2819 3230 1199
Size: 521 678 274 147 411 1979
Fragments arranged by size:
1979 678 521 411 274 147
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 832 832
Size: 4010
BseYI C'CCAG_C
Cuts at: 1435 2193 2770 3266 1435
Size: 758 577 496 2179
Fragments arranged by size:
2179 758 577 496
BsgI GTGCAGnnnnnnnnnnnnnn_nn'
Cuts at: 853 853
Size: 4010
BsiEI CG_ry'CG
Cuts at: 104 1552 1598 2806 104
Size: 1448 46 1208 1308
Fragments arranged by size:
1448 1308 1208 46
BsiHKAI G_wGCw'C
Cuts at: 134 326 1024 1802 1992 2784 134
Size: 192 698 778 190 792 1360
Fragments arranged by size:
1360 792 778 698 192 190
Bsp1286I G_dGCh'C
Cuts at: 134 326 1024 1635 1728 1802 1992 2054 2784
Size: 192 698 611 93 74 190 62 730
Cuts at: 2784 134
Size: 1360
Fragments arranged by size:
1360 730 698 611 192 190 93 74
62
BspEI T'CCGG_A
Cuts at: 3230 3230
Size: 4010
BspHI T'CATG_A
Cuts at: 3186 3186
Size: 4010
BspMI ACCTGCnnnn'nnnn_
Cuts at: 1060 1274 1576 1957 1060
Size: 214 302 381 3113
Fragments arranged by size:
3113 381 302 214
BsrBI CCG'CTC
Cuts at: 50 2302 2356 3840 4005 50
Size: 2252 54 1484 165 55
Fragments arranged by size:
2252 1484 165 55 54
BsrDI GCAATG_nn'
Cuts at: 1922 1922
Size: 4010
BsrFI r'CCGG_y
Cuts at: 2008 2189 2398 2008
Size: 181 209 3620
Fragments arranged by size:
3620 209 181
BssSI C'ACGA_G
Cuts at: 2281 2639 2281
Size: 358 3652
BstAPI GCAn_nnn'nTGC
Cuts at: 1387 1387
Size: 4010
BstBI TT'CG_AA
Cuts at: 2371 2371
Size: 4010
BstYI r'GATC_y
Cuts at: 1 966 1035 1502 1525 1860 2106 3118 3204
Size: 965 69 467 23 335 246 1012 86
Cuts at: 3204 3216 3562 1
Size: 12 346 449
Fragments arranged by size:
1012 965 467 449 346 335 246 86
69 23 12
BtgI C'CryG_G
Cuts at: 107 107
Size: 4010
BtgZI GCGATGnnnnnnnnnn'nnnn_
Cuts at: 1960 2141 2304 1960
Size: 181 163 3666
Fragments arranged by size:
3666 181 163
BtsI GCAGTG_nn'
Cuts at: 332 670 1253 332
Size: 338 583 3089
Fragments arranged by size:
3089 583 338
DraI TTT'AAA
Cuts at: 1009 1009
Size: 4010
DrdI GACnn_nn'nnGTC
Cuts at: 1716 2574 1716
Size: 858 3152
EaeI y'GGCC_r
Cuts at: 1595 1769 2160 2187 3532 1595
Size: 174 391 27 1345 2073
Fragments arranged by size:
2073 1345 391 174 27
EagI C'GGCC_G
Cuts at: 1595 1595
Size: 4010
EarI CTCTTCn'nnn_
Cuts at: 578 2033 2243 578
Size: 1455 210 2345
Fragments arranged by size:
2345 1455 210
EciI GGCGGAnnnnnnnnn_nn'
Cuts at: 2218 2528 2674 3820 2218
Size: 310 146 1146 2408
Fragments arranged by size:
2408 1146 310 146
EcoICRI GAG'CTC
Cuts at: 324 1022 324
Size: 698 3312
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 293 835 1019 1835 2267 3014 3282 293
Size: 542 184 816 432 747 268 1021
Fragments arranged by size:
1021 816 747 542 432 268 184
EcoNI CCTnn'n_nnAGG
Cuts at: 559 559
Size: 4010
EcoO109I rG'GnC_Cy
Cuts at: 1149 1149
Size: 4010
EcoRI G'AATT_C
Cuts at: 1014 1014
Size: 4010
FalI AAGnnnnnCTTnnnnnnnn_nnnnn'
Cuts at: 201 513 955 2029 201
Size: 312 442 1074 2182
Fragments arranged by size:
2182 1074 442 312
FspI TGC'GCA
Cuts at: 1791 1791
Size: 4010
HaeII r_GCGC'y
Cuts at: 1444 1692 2714 3693 1444
Size: 248 1022 979 1761
Fragments arranged by size:
1761 1022 979 248
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 253 285 301 333 460 492 637 669 1852
Size: 32 16 32 127 32 145 32 1183
Cuts at: 1852 1882 1884 1914 253
Size: 30 2 30 2349
Fragments arranged by size:
2349 1183 145 127 32 32 32 32
30 30 16 2
HincII GTy'rAC
Cuts at: 1049 1049
Size: 4010
KasI G'GCGC_C
Cuts at: 1440 1688 1440
Size: 248 3762
KpnI G_GTAC'C
Cuts at: 1030 1030
Size: 4010
MluI A'CGCG_T
Cuts at: 3443 3443
Size: 4010
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 2681 2865 2681
Size: 184 3826
MscI TGG'CCA
Cuts at: 1771 1771
Size: 4010
MslI CAynn'nnrTG
Cuts at: 140 1999 2126 140
Size: 1859 127 2024
Fragments arranged by size:
2024 1859 127
MspA1I CmG'CkG
Cuts at: 366 867 1121 1435 1795 2808 3053 366
Size: 501 254 314 360 1013 245 1323
Fragments arranged by size:
1323 1013 501 360 314 254 245
NaeI GCC'GGC
Cuts at: 2191 2191
Size: 4010
NarI GG'CG_CC
Cuts at: 1441 1689 1441
Size: 248 3762
NcoI C'CATG_G
Cuts at: 107 107
Size: 4010
NgoMIV G'CCGG_C
Cuts at: 2189 2189
Size: 4010
NspI r_CATG'y
Cuts at: 1063 1563 2470 1063
Size: 500 907 2603
Fragments arranged by size:
2603 907 500
PciI A'CATG_T
Cuts at: 2466 2466
Size: 4010
PmeI GTTT'AAAC
Cuts at: 1009 1009
Size: 4010
PpiI GAACnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 3149 3181 3149
Size: 32 3978
PsiI TTA'TAA
Cuts at: 715 715
Size: 4010
PstI C_TGCA'G
Cuts at: 1057 1742 1057
Size: 685 3325
PvuI CG_AT'CG
Cuts at: 104 104
Size: 4010
PvuII CAG'CTG
Cuts at: 366 867 1435 1795 366
Size: 501 568 360 2581
Fragments arranged by size:
2581 568 501 360
RsrII CG'GwC_CG
Cuts at: 2205 2205
Size: 4010
SacI G_AGCT'C
Cuts at: 326 1024 326
Size: 698 3312
SalI G'TCGA_C
Cuts at: 1047 1047
Size: 4010
SapI GCTCTTCn'nnn_
Cuts at: 2033 2243 2033
Size: 210 3800
SbfI CC_TGCA'GG
Cuts at: 1057 1057
Size: 4010
ScaI AGT'ACT
Cuts at: 3527 3527
Size: 4010
SfcI C'TryA_G
Cuts at: 33 200 400 1053 1738 2731 2922 33
Size: 167 200 653 685 993 191 1121
Fragments arranged by size:
1121 993 685 653 200 191 167
SfoI GGC'GCC
Cuts at: 1442 1690 1442
Size: 248 3762
SgfI GCG_AT'CGC
Cuts at: 104 104
Size: 4010
SmaI CCC'GGG
Cuts at: 1032 1032
Size: 4010
SmlI C'TyrA_G
Cuts at: 186 223 394 729 1165 2080 2572 2834 186
Size: 37 171 335 436 915 492 262 1362
Fragments arranged by size:
1362 915 492 436 335 262 171 37
SpeI A'CTAG_T
Cuts at: 67 67
Size: 4010
SphI G_CATG'C
Cuts at: 1063 1563 1063
Size: 500 3510
StyI C'CwwG_G
Cuts at: 107 1144 1498 107
Size: 1037 354 2619
Fragments arranged by size:
2619 1037 354
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 1593 1726 2185 2394 1593
Size: 133 459 209 3209
Fragments arranged by size:
3209 459 209 133
TatI w'GTAC_w
Cuts at: 3525 3525
Size: 4010
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 3527 3559 3527
Size: 32 3978
Tth111I GACn'n_nGTC
Cuts at: 1807 1807
Size: 4010
XbaI T'CTAG_A
Cuts at: 1041 1041
Size: 4010
XcmI CCAnnnn_n'nnnnTGG
Cuts at: 114 1505 114
Size: 1391 2619
XmaI C'CCGG_G
Cuts at: 1030 1030
Size: 4010
Enzymes that do cut:
AccI Acc65I AclI AcuI AflII AflIII AgeI AlwNI
ApaLI ApoI AseI AsiSI AvaI BamHI BanI BanII
BbeI BbsI BciVI BclI BglII BlpI Bme1580I BmrI
BplI BpmI BpuEI BsaI BsaAI BsaBI BsaHI BsaWI
BseRI BseYI BsgI BsiEI BsiHKAI Bsp1286I BspEI BspHI
BspMI BsrBI BsrDI BsrFI BssSI BstAPI BstBI BstYI
BtgI BtgZI BtsI DraI DrdI EaeI EagI EarI
EciI EcoICRI Eco57MI EcoNI EcoO109I EcoRI FalI FspI
HaeII Hin4I HincII KasI KpnI MluI MmeI MscI
MslI MspA1I NaeI NarI NcoI NgoMIV NspI PciI
PmeI PpiI PsiI PstI PvuI PvuII RsrII SacI
SalI SapI SbfI ScaI SfcI SfoI SgfI SmaI
SmlI SpeI SphI StyI TaqII TatI TstI Tth111I
XbaI XcmI XmaI
Enzymes that do not cut:
AarI AatII AfeI AhdI AleI AlfI AloI ApaI
AscI AvrII BaeI BbvCI BcgI BfrBI BglI BmgBI
BmtI Bpu10I BsaXI BsiWI BsmI BsmBI BsrGI BssHII
BstEII BstXI BstZ17I Bsu36I ClaI CspCI DraIII EcoRV
FseI FspAI HindIII HpaI MfeI NdeI NheI NotI
NruI NsiI PacI PasI PflMI PfoI PmlI PpuMI
PshAI PspOMI PspXI PsrI SacII SanDI SexAI SfiI
SgrAI SnaBI SrfI SspI StuI SwaI XhoI XmnI
ZraI