Restriction map for
pF1KB7603
(Circular) (Six-base) MAPSORT of: pF1KB7603.seq Check: 9824 from: 1 to: 4004
pF1KB7603 4004 bp
With 182 enzymes: SgfI *
April 24, 2009 13:08 ..
AccI GT'mk_AC
Cuts at: 1042 1042
Size: 4004
Acc65I G'GTAC_C
Cuts at: 314 799 1020 314
Size: 485 221 3298
Fragments arranged by size:
3298 485 221
AclI AA'CG_TT
Cuts at: 3844 3844
Size: 4004
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 662 1829 2261 3008 3276 662
Size: 1167 432 747 268 1390
Fragments arranged by size:
1390 1167 747 432 268
AflII C'TTAA_G
Cuts at: 121 121
Size: 4004
AflIII A'CryG_T
Cuts at: 1356 2460 3437 1356
Size: 1104 977 1923
Fragments arranged by size:
1923 1104 977
AgeI A'CCGG_T
Cuts at: 2392 2392
Size: 4004
AlwNI CAG_nnn'CTG
Cuts at: 1309 2876 1309
Size: 1567 2437
ApaI G_GGCC'C
Cuts at: 209 209
Size: 4004
ApaLI G'TGCA_C
Cuts at: 2774 2774
Size: 4004
ApoI r'AATT_y
Cuts at: 985 1008 3357 3613 985
Size: 23 2349 256 1376
Fragments arranged by size:
2349 1376 256 23
AseI AT'TA_AT
Cuts at: 20 20
Size: 4004
AsiSI GCG_AT'CGC
Cuts at: 104 104
Size: 4004
AvaI C'yCGr_G
Cuts at: 201 269 364 485 1024 201
Size: 68 95 121 539 3181
Fragments arranged by size:
3181 539 121 95 68
BamHI G'GATC_C
Cuts at: 1029 1029
Size: 4004
BanI G'GyrC_C
Cuts at: 314 799 1020 1434 1682 1717 314
Size: 485 221 414 248 35 2601
Fragments arranged by size:
2601 485 414 248 221 35
BanII G_rGCy'C
Cuts at: 209 592 1018 2048 209
Size: 383 426 1030 2165
Fragments arranged by size:
2165 1030 426 383
BbeI G_GCGC'C
Cuts at: 1438 1686 1438
Size: 248 3756
BbsI GAAGACnn'nnnn_
Cuts at: 478 478
Size: 4004
BciVI GTATCCnnnnn_n'
Cuts at: 1902 2663 4004 1902
Size: 761 1341 1902
Fragments arranged by size:
1902 1341 761
BclI T'GATC_A
Cuts at: 1524 1524
Size: 4004
BfrBI ATG'CAT
Cuts at: 756 756
Size: 4004
BglII A'GATC_T
Cuts at: 1519 3556 1519
Size: 2037 1967
BlpI GC'TnA_GC
Cuts at: 1116 1116
Size: 4004
Bme1580I G_kGCm'C
Cuts at: 209 1629 1722 2778 209
Size: 1420 93 1056 1435
Fragments arranged by size:
1435 1420 1056 93
BmrI ACTGGGnnnn_n'
Cuts at: 1216 1297 1632 3776 1216
Size: 81 335 2144 1444
Fragments arranged by size:
2144 1444 335 81
BplI GAGnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 2371 2371
Size: 4004
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 464 606 464
Size: 142 3862
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 1180 2059 2551 2849 1180
Size: 879 492 298 2335
Fragments arranged by size:
2335 879 492 298
BsaI GGTCTCn'nnnn_
Cuts at: 450 3708 450
Size: 3258 746
BsaAI yAC'GTr
Cuts at: 1357 1357
Size: 4004
BsaBI GATnn'nnATC
Cuts at: 6 1518 3555 6
Size: 1512 2037 455
Fragments arranged by size:
2037 1512 455
BsaHI Gr'CG_yC
Cuts at: 1435 1683 2385 3881 1435
Size: 248 702 1496 1558
Fragments arranged by size:
1558 1496 702 248
BsaWI w'CCGG_w
Cuts at: 1193 1714 2392 2666 2813 3224 1193
Size: 521 678 274 147 411 1973
Fragments arranged by size:
1973 678 521 411 274 147
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 352 621 352
Size: 269 3735
BseYI C'CCAG_C
Cuts at: 591 834 1429 2187 2764 3260 591
Size: 243 595 758 577 496 1335
Fragments arranged by size:
1335 758 595 577 496 243
BsgI GTGCAGnnnnnnnnnnnnnn_nn'
Cuts at: 166 397 166
Size: 231 3773
BsiEI CG_ry'CG
Cuts at: 104 1546 1592 2800 104
Size: 1442 46 1208 1308
Fragments arranged by size:
1442 1308 1208 46
BsiHKAI G_wGCw'C
Cuts at: 573 1018 1796 1986 2778 573
Size: 445 778 190 792 1799
Fragments arranged by size:
1799 792 778 445 190
Bsp1286I G_dGCh'C
Cuts at: 209 573 592 1018 1629 1722 1796 1986 2048
Size: 364 19 426 611 93 74 190 62
Cuts at: 2048 2778 209
Size: 730 1435
Fragments arranged by size:
1435 730 611 426 364 190 93 74
62 19
BspEI T'CCGG_A
Cuts at: 3224 3224
Size: 4004
BspHI T'CATG_A
Cuts at: 3180 3180
Size: 4004
BspMI ACCTGCnnnn'nnnn_
Cuts at: 369 468 1054 1268 1570 1951 369
Size: 99 586 214 302 381 2422
Fragments arranged by size:
2422 586 381 302 214 99
BsrBI CCG'CTC
Cuts at: 50 2296 2350 3834 3999 50
Size: 2246 54 1484 165 55
Fragments arranged by size:
2246 1484 165 55 54
BsrDI GCAATG_nn'
Cuts at: 554 1916 554
Size: 1362 2642
BsrFI r'CCGG_y
Cuts at: 2002 2183 2392 2002
Size: 181 209 3614
Fragments arranged by size:
3614 209 181
BssSI C'ACGA_G
Cuts at: 978 2275 2633 978
Size: 1297 358 2349
Fragments arranged by size:
2349 1297 358
BstAPI GCAn_nnn'nTGC
Cuts at: 301 1381 301
Size: 1080 2924
BstBI TT'CG_AA
Cuts at: 2365 2365
Size: 4004
BstXI CCAn_nnnn'nTGG
Cuts at: 859 859
Size: 4004
BstYI r'GATC_y
Cuts at: 1 615 1029 1496 1519 1854 2100 3112 3198
Size: 614 414 467 23 335 246 1012 86
Cuts at: 3198 3210 3556 1
Size: 12 346 449
Fragments arranged by size:
1012 614 467 449 414 346 335 246
86 23 12
BtgI C'CryG_G
Cuts at: 191 794 191
Size: 603 3401
BtgZI GCGATGnnnnnnnnnn'nnnn_
Cuts at: 1954 2135 2298 1954
Size: 181 163 3660
Fragments arranged by size:
3660 181 163
BtsI GCAGTG_nn'
Cuts at: 573 1247 573
Size: 674 3330
DraI TTT'AAA
Cuts at: 1003 1003
Size: 4004
DrdI GACnn_nn'nnGTC
Cuts at: 1710 2568 1710
Size: 858 3146
EaeI y'GGCC_r
Cuts at: 516 1589 1763 2154 2181 3526 516
Size: 1073 174 391 27 1345 994
Fragments arranged by size:
1345 1073 994 391 174 27
EagI C'GGCC_G
Cuts at: 1589 1589
Size: 4004
EarI CTCTTCn'nnn_
Cuts at: 393 639 2027 2237 393
Size: 246 1388 210 2160
Fragments arranged by size:
2160 1388 246 210
EciI GGCGGAnnnnnnnnn_nn'
Cuts at: 2212 2522 2668 3814 2212
Size: 310 146 1146 2402
Fragments arranged by size:
2402 1146 310 146
EcoICRI GAG'CTC
Cuts at: 1016 1016
Size: 4004
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 464 606 662 1829 2261 3008 3276 464
Size: 142 56 1167 432 747 268 1192
Fragments arranged by size:
1192 1167 747 432 268 142 56
EcoO109I rG'GnC_Cy
Cuts at: 205 206 650 1143 205
Size: 1 444 493 3066
Fragments arranged by size:
3066 493 444 1
EcoRI G'AATT_C
Cuts at: 1008 1008
Size: 4004
FalI AAGnnnnnCTTnnnnnnnn_nnnnn'
Cuts at: 954 2023 954
Size: 1069 2935
FspI TGC'GCA
Cuts at: 1785 1785
Size: 4004
HaeII r_GCGC'y
Cuts at: 249 1438 1686 2708 3687 249
Size: 1189 248 1022 979 566
Fragments arranged by size:
1189 1022 979 566 248
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 102 134 252 284 607 639 654 671 686
Size: 32 118 32 323 32 15 17 15
Cuts at: 686 721 753 958 990 1846 1876 1878 1908
Size: 35 32 205 32 856 30 2 30
Cuts at: 1908 102
Size: 2198
Fragments arranged by size:
2198 856 323 205 118 35 32 32
32 32 32 30 30 17 15 15
2
HincII GTy'rAC
Cuts at: 1043 1043
Size: 4004
HindIII A'AGCT_T
Cuts at: 870 931 870
Size: 61 3943
KasI G'GCGC_C
Cuts at: 1434 1682 1434
Size: 248 3756
KpnI G_GTAC'C
Cuts at: 318 803 1024 318
Size: 485 221 3298
Fragments arranged by size:
3298 485 221
MluI A'CGCG_T
Cuts at: 3437 3437
Size: 4004
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 2675 2859 2675
Size: 184 3820
MscI TGG'CCA
Cuts at: 518 1765 518
Size: 1247 2757
MslI CAynn'nnrTG
Cuts at: 328 978 1993 2120 328
Size: 650 1015 127 2212
Fragments arranged by size:
2212 1015 650 127
MspA1I CmG'CkG
Cuts at: 1115 1429 1789 2802 3047 1115
Size: 314 360 1013 245 2072
Fragments arranged by size:
2072 1013 360 314 245
NaeI GCC'GGC
Cuts at: 2185 2185
Size: 4004
NarI GG'CG_CC
Cuts at: 1435 1683 1435
Size: 248 3756
NcoI C'CATG_G
Cuts at: 794 794
Size: 4004
NgoMIV G'CCGG_C
Cuts at: 2183 2183
Size: 4004
NsiI A_TGCA'T
Cuts at: 758 758
Size: 4004
NspI r_CATG'y
Cuts at: 645 1057 1557 2464 645
Size: 412 500 907 2185
Fragments arranged by size:
2185 907 500 412
PasI CC'CwG_GG
Cuts at: 209 852 209
Size: 643 3361
PciI A'CATG_T
Cuts at: 2460 2460
Size: 4004
PflMI CCAn_nnn'nTGG
Cuts at: 996 996
Size: 4004
PfoI T'CCnGG_A
Cuts at: 768 925 768
Size: 157 3847
PmeI GTTT'AAAC
Cuts at: 1003 1003
Size: 4004
PpiI GAACnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 3143 3175 3143
Size: 32 3972
PpuMI rG'GwC_Cy
Cuts at: 650 650
Size: 4004
PsiI TTA'TAA
Cuts at: 908 962 908
Size: 54 3950
PspOMI G'GGCC_C
Cuts at: 205 205
Size: 4004
PstI C_TGCA'G
Cuts at: 1051 1736 1051
Size: 685 3319
PvuI CG_AT'CG
Cuts at: 104 104
Size: 4004
PvuII CAG'CTG
Cuts at: 1429 1789 1429
Size: 360 3644
RsrII CG'GwC_CG
Cuts at: 2199 2199
Size: 4004
SacI G_AGCT'C
Cuts at: 1018 1018
Size: 4004
SalI G'TCGA_C
Cuts at: 1041 1041
Size: 4004
SapI GCTCTTCn'nnn_
Cuts at: 393 2027 2237 393
Size: 1634 210 2160
Fragments arranged by size:
2160 1634 210
SbfI CC_TGCA'GG
Cuts at: 1051 1051
Size: 4004
ScaI AGT'ACT
Cuts at: 3521 3521
Size: 4004
SfcI C'TryA_G
Cuts at: 33 1047 1732 2725 2916 33
Size: 1014 685 993 191 1121
Fragments arranged by size:
1121 1014 993 685 191
SfoI GGC'GCC
Cuts at: 1436 1684 1436
Size: 248 3756
SgfI GCG_AT'CGC
Cuts at: 104 104
Size: 4004
SmaI CCC'GGG
Cuts at: 203 1026 203
Size: 823 3181
SmlI C'TyrA_G
Cuts at: 121 1159 2074 2566 2828 121
Size: 1038 915 492 262 1297
Fragments arranged by size:
1297 1038 915 492 262
SpeI A'CTAG_T
Cuts at: 67 67
Size: 4004
SphI G_CATG'C
Cuts at: 645 1057 1557 645
Size: 412 500 3092
Fragments arranged by size:
3092 500 412
StyI C'CwwG_G
Cuts at: 794 1138 1492 794
Size: 344 354 3306
Fragments arranged by size:
3306 354 344
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 865 1587 1720 2179 2388 865
Size: 722 133 459 209 2481
Fragments arranged by size:
2481 722 459 209 133
TatI w'GTAC_w
Cuts at: 3519 3519
Size: 4004
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 774 806 3521 3553 774
Size: 32 2715 32 1225
Fragments arranged by size:
2715 1225 32 32
Tth111I GACn'n_nGTC
Cuts at: 1801 1801
Size: 4004
XbaI T'CTAG_A
Cuts at: 1035 1035
Size: 4004
XcmI CCAnnnn_n'nnnnTGG
Cuts at: 168 897 1499 168
Size: 729 602 2673
Fragments arranged by size:
2673 729 602
XmaI C'CCGG_G
Cuts at: 201 1024 201
Size: 823 3181
Enzymes that do cut:
AccI Acc65I AclI AcuI AflII AflIII AgeI AlwNI
ApaI ApaLI ApoI AseI AsiSI AvaI BamHI BanI
BanII BbeI BbsI BciVI BclI BfrBI BglII BlpI
Bme1580I BmrI BplI BpmI BpuEI BsaI BsaAI BsaBI
BsaHI BsaWI BseRI BseYI BsgI BsiEI BsiHKAI Bsp1286I
BspEI BspHI BspMI BsrBI BsrDI BsrFI BssSI BstAPI
BstBI BstXI BstYI BtgI BtgZI BtsI DraI DrdI
EaeI EagI EarI EciI EcoICRI Eco57MI EcoO109I EcoRI
FalI FspI HaeII Hin4I HincII HindIII KasI KpnI
MluI MmeI MscI MslI MspA1I NaeI NarI NcoI
NgoMIV NsiI NspI PasI PciI PflMI PfoI PmeI
PpiI PpuMI PsiI PspOMI PstI PvuI PvuII RsrII
SacI SalI SapI SbfI ScaI SfcI SfoI SgfI
SmaI SmlI SpeI SphI StyI TaqII TatI TstI
Tth111I XbaI XcmI XmaI
Enzymes that do not cut:
AarI AatII AfeI AhdI AleI AlfI AloI AscI
AvrII BaeI BbvCI BcgI BglI BmgBI BmtI Bpu10I
BsaXI BsiWI BsmI BsmBI BsrGI BssHII BstEII BstZ17I
Bsu36I ClaI CspCI DraIII EcoNI EcoRV FseI FspAI
HpaI MfeI NdeI NheI NotI NruI PacI PmlI
PshAI PspXI PsrI SacII SanDI SexAI SfiI SgrAI
SnaBI SrfI SspI StuI SwaI XhoI XmnI ZraI