Restriction map for
pF1KB7591
(Circular) (Six-base) MAPSORT of: pF1KB7591.seq Check: 2024 from: 1 to: 3791
pF1KB7591 3791 bp
With 182 enzymes: SgfI *
April 24, 2009 13:08 ..
AccI GT'mk_AC
Cuts at: 829 829
Size: 3791
Acc65I G'GTAC_C
Cuts at: 689 807 689
Size: 118 3673
AclI AA'CG_TT
Cuts at: 785 3631 785
Size: 2846 945
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 318 487 1616 2048 2795 3063 318
Size: 169 1129 432 747 268 1046
Fragments arranged by size:
1129 1046 747 432 268 169
AfeI AGC'GCT
Cuts at: 493 493
Size: 3791
AflIII A'CryG_T
Cuts at: 1143 2247 3224 1143
Size: 1104 977 1710
Fragments arranged by size:
1710 1104 977
AgeI A'CCGG_T
Cuts at: 2179 2179
Size: 3791
AleI CACnn'nnGTG
Cuts at: 211 211
Size: 3791
AlfI GCAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 187 187
Size: 3791
AlwNI CAG_nnn'CTG
Cuts at: 510 712 1096 2663 510
Size: 202 384 1567 1638
Fragments arranged by size:
1638 1567 384 202
ApaI G_GGCC'C
Cuts at: 402 402
Size: 3791
ApaLI G'TGCA_C
Cuts at: 2561 2561
Size: 3791
ApoI r'AATT_y
Cuts at: 795 3144 3400 795
Size: 2349 256 1186
Fragments arranged by size:
2349 1186 256
AseI AT'TA_AT
Cuts at: 20 20
Size: 3791
AsiSI GCG_AT'CGC
Cuts at: 104 104
Size: 3791
AvaI C'yCGr_G
Cuts at: 219 401 416 811 219
Size: 182 15 395 3199
Fragments arranged by size:
3199 395 182 15
BamHI G'GATC_C
Cuts at: 816 816
Size: 3791
BanI G'GyrC_C
Cuts at: 125 314 655 689 807 1221 1469 1504 125
Size: 189 341 34 118 414 248 35 2412
Fragments arranged by size:
2412 414 341 248 189 118 35 34
BanII G_rGCy'C
Cuts at: 402 805 1835 402
Size: 403 1030 2358
Fragments arranged by size:
2358 1030 403
BbeI G_GCGC'C
Cuts at: 129 318 659 1225 1473 129
Size: 189 341 566 248 2447
Fragments arranged by size:
2447 566 341 248 189
BbvCI CC'TCA_GC
Cuts at: 757 757
Size: 3791
BciVI GTATCCnnnnn_n'
Cuts at: 1689 2450 3791 1689
Size: 761 1341 1689
Fragments arranged by size:
1689 1341 761
BclI T'GATC_A
Cuts at: 1311 1311
Size: 3791
BglII A'GATC_T
Cuts at: 1306 3343 1306
Size: 2037 1754
BlpI GC'TnA_GC
Cuts at: 473 903 473
Size: 430 3361
Bme1580I G_kGCm'C
Cuts at: 402 1416 1509 2565 402
Size: 1014 93 1056 1628
Fragments arranged by size:
1628 1056 1014 93
BmrI ACTGGGnnnn_n'
Cuts at: 203 334 1003 1084 1419 3563 203
Size: 131 669 81 335 2144 431
Fragments arranged by size:
2144 669 431 335 131 81
BplI GAGnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 2158 2158
Size: 3791
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 379 379
Size: 3791
Bpu10I CC'TnA_GC
Cuts at: 757 757
Size: 3791
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 784 967 1846 2338 2636 784
Size: 183 879 492 298 1939
Fragments arranged by size:
1939 879 492 298 183
BsaI GGTCTCn'nnnn_
Cuts at: 3495 3495
Size: 3791
BsaAI yAC'GTr
Cuts at: 1144 1144
Size: 3791
BsaBI GATnn'nnATC
Cuts at: 6 1305 3342 6
Size: 1299 2037 455
Fragments arranged by size:
2037 1299 455
BsaHI Gr'CG_yC
Cuts at: 126 315 656 1222 1470 2172 3668 126
Size: 189 341 566 248 702 1496 249
Fragments arranged by size:
1496 702 566 341 249 248 189
BsaWI w'CCGG_w
Cuts at: 980 1501 2179 2453 2600 3011 980
Size: 521 678 274 147 411 1760
Fragments arranged by size:
1760 678 521 411 274 147
BseYI C'CCAG_C
Cuts at: 638 705 1216 1974 2551 3047 638
Size: 67 511 758 577 496 1382
Fragments arranged by size:
1382 758 577 511 496 67
BsgI GTGCAGnnnnnnnnnnnnnn_nn'
Cuts at: 272 364 272
Size: 92 3699
BsiEI CG_ry'CG
Cuts at: 104 1333 1379 2587 104
Size: 1229 46 1208 1308
Fragments arranged by size:
1308 1229 1208 46
BsiHKAI G_wGCw'C
Cuts at: 228 805 1583 1773 2565 228
Size: 577 778 190 792 1454
Fragments arranged by size:
1454 792 778 577 190
Bsp1286I G_dGCh'C
Cuts at: 228 402 805 1416 1509 1583 1773 1835 2565
Size: 174 403 611 93 74 190 62 730
Cuts at: 2565 228
Size: 1454
Fragments arranged by size:
1454 730 611 403 190 174 93 74
62
BspEI T'CCGG_A
Cuts at: 3011 3011
Size: 3791
BspHI T'CATG_A
Cuts at: 2967 2967
Size: 3791
BspMI ACCTGCnnnn'nnnn_
Cuts at: 610 841 1055 1357 1738 610
Size: 231 214 302 381 2663
Fragments arranged by size:
2663 381 302 231 214
BsrBI CCG'CTC
Cuts at: 50 2083 2137 3621 3786 50
Size: 2033 54 1484 165 55
Fragments arranged by size:
2033 1484 165 55 54
BsrDI GCAATG_nn'
Cuts at: 1703 1703
Size: 3791
BsrFI r'CCGG_y
Cuts at: 715 1789 1970 2179 715
Size: 1074 181 209 2327
Fragments arranged by size:
2327 1074 209 181
BssSI C'ACGA_G
Cuts at: 2062 2420 2062
Size: 358 3433
BstAPI GCAn_nnn'nTGC
Cuts at: 1168 1168
Size: 3791
BstBI TT'CG_AA
Cuts at: 2152 2152
Size: 3791
BstXI CCAn_nnnn'nTGG
Cuts at: 213 213
Size: 3791
BstYI r'GATC_y
Cuts at: 1 816 1283 1306 1641 1887 2899 2985 2997
Size: 815 467 23 335 246 1012 86 12
Cuts at: 2997 3343 1
Size: 346 449
Fragments arranged by size:
1012 815 467 449 346 335 246 86
23 12
BtgI C'CryG_G
Cuts at: 107 209 107
Size: 102 3689
BtgZI GCGATGnnnnnnnnnn'nnnn_
Cuts at: 1741 1922 2085 1741
Size: 181 163 3447
Fragments arranged by size:
3447 181 163
BtsI GCAGTG_nn'
Cuts at: 496 557 1034 496
Size: 61 477 3253
Fragments arranged by size:
3253 477 61
DraI TTT'AAA
Cuts at: 790 790
Size: 3791
DrdI GACnn_nn'nnGTC
Cuts at: 1497 2355 1497
Size: 858 2933
EaeI y'GGCC_r
Cuts at: 188 717 1376 1550 1941 1968 3313 188
Size: 529 659 174 391 27 1345 666
Fragments arranged by size:
1345 666 659 529 391 174 27
EagI C'GGCC_G
Cuts at: 1376 1376
Size: 3791
EarI CTCTTCn'nnn_
Cuts at: 1814 2024 1814
Size: 210 3581
EciI GGCGGAnnnnnnnnn_nn'
Cuts at: 1999 2309 2455 3601 1999
Size: 310 146 1146 2189
Fragments arranged by size:
2189 1146 310 146
EcoICRI GAG'CTC
Cuts at: 803 803
Size: 3791
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 318 379 487 1616 2048 2795 3063 318
Size: 61 108 1129 432 747 268 1046
Fragments arranged by size:
1129 1046 747 432 268 108 61
EcoNI CCTnn'n_nnAGG
Cuts at: 237 251 372 433 237
Size: 14 121 61 3595
Fragments arranged by size:
3595 121 61 14
EcoO109I rG'GnC_Cy
Cuts at: 197 930 197
Size: 733 3058
EcoRI G'AATT_C
Cuts at: 795 795
Size: 3791
FalI AAGnnnnnCTTnnnnnnnn_nnnnn'
Cuts at: 1810 1810
Size: 3791
FspI TGC'GCA
Cuts at: 1572 1572
Size: 3791
HaeII r_GCGC'y
Cuts at: 129 318 495 659 1225 1473 2495 3474 129
Size: 189 177 164 566 248 1022 979 446
Fragments arranged by size:
1022 979 566 446 248 189 177 164
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 1633 1663 1665 1695 1633
Size: 30 2 30 3729
Fragments arranged by size:
3729 30 30 2
HincII GTy'rAC
Cuts at: 830 830
Size: 3791
KasI G'GCGC_C
Cuts at: 125 314 655 1221 1469 125
Size: 189 341 566 248 2447
Fragments arranged by size:
2447 566 341 248 189
KpnI G_GTAC'C
Cuts at: 693 811 693
Size: 118 3673
MluI A'CGCG_T
Cuts at: 3224 3224
Size: 3791
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 329 2462 2646 329
Size: 2133 184 1474
Fragments arranged by size:
2133 1474 184
MscI TGG'CCA
Cuts at: 1552 1552
Size: 3791
MslI CAynn'nnrTG
Cuts at: 211 1780 1907 211
Size: 1569 127 2095
Fragments arranged by size:
2095 1569 127
MspA1I CmG'CkG
Cuts at: 357 902 1216 1576 2589 2834 357
Size: 545 314 360 1013 245 1314
Fragments arranged by size:
1314 1013 545 360 314 245
NaeI GCC'GGC
Cuts at: 717 1972 717
Size: 1255 2536
NarI GG'CG_CC
Cuts at: 126 315 656 1222 1470 126
Size: 189 341 566 248 2447
Fragments arranged by size:
2447 566 341 248 189
NcoI C'CATG_G
Cuts at: 107 107
Size: 3791
NgoMIV G'CCGG_C
Cuts at: 715 1970 715
Size: 1255 2536
NspI r_CATG'y
Cuts at: 844 1344 2251 844
Size: 500 907 2384
Fragments arranged by size:
2384 907 500
PciI A'CATG_T
Cuts at: 2247 2247
Size: 3791
PflMI CCAn_nnn'nTGG
Cuts at: 779 779
Size: 3791
PfoI T'CCnGG_A
Cuts at: 778 778
Size: 3791
PmeI GTTT'AAAC
Cuts at: 790 790
Size: 3791
PpiI GAACnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 2930 2962 2930
Size: 32 3759
PpuMI rG'GwC_Cy
Cuts at: 197 197
Size: 3791
PspOMI G'GGCC_C
Cuts at: 398 398
Size: 3791
PspXI vC'TCGA_Gb
Cuts at: 219 219
Size: 3791
PstI C_TGCA'G
Cuts at: 238 838 1523 238
Size: 600 685 2506
Fragments arranged by size:
2506 685 600
PvuI CG_AT'CG
Cuts at: 104 104
Size: 3791
PvuII CAG'CTG
Cuts at: 357 1216 1576 357
Size: 859 360 2572
Fragments arranged by size:
2572 859 360
RsrII CG'GwC_CG
Cuts at: 1986 1986
Size: 3791
SacI G_AGCT'C
Cuts at: 805 805
Size: 3791
SalI G'TCGA_C
Cuts at: 828 828
Size: 3791
SanDI GG'GwC_CC
Cuts at: 197 197
Size: 3791
SapI GCTCTTCn'nnn_
Cuts at: 1814 2024 1814
Size: 210 3581
SbfI CC_TGCA'GG
Cuts at: 838 838
Size: 3791
ScaI AGT'ACT
Cuts at: 3308 3308
Size: 3791
SfcI C'TryA_G
Cuts at: 33 234 618 834 1519 2512 2703 33
Size: 201 384 216 685 993 191 1121
Fragments arranged by size:
1121 993 685 384 216 201 191
SfoI GGC'GCC
Cuts at: 127 316 657 1223 1471 127
Size: 189 341 566 248 2447
Fragments arranged by size:
2447 566 341 248 189
SgfI GCG_AT'CGC
Cuts at: 104 104
Size: 3791
SmaI CCC'GGG
Cuts at: 418 813 418
Size: 395 3396
SmlI C'TyrA_G
Cuts at: 219 763 946 1861 2353 2615 219
Size: 544 183 915 492 262 1395
Fragments arranged by size:
1395 915 544 492 262 183
SpeI A'CTAG_T
Cuts at: 67 67
Size: 3791
SphI G_CATG'C
Cuts at: 844 1344 844
Size: 500 3291
StyI C'CwwG_G
Cuts at: 107 925 1279 107
Size: 818 354 2619
Fragments arranged by size:
2619 818 354
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 719 1374 1507 1966 2175 719
Size: 655 133 459 209 2335
Fragments arranged by size:
2335 655 459 209 133
TatI w'GTAC_w
Cuts at: 3306 3306
Size: 3791
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 3308 3340 3308
Size: 32 3759
Tth111I GACn'n_nGTC
Cuts at: 1588 1588
Size: 3791
XbaI T'CTAG_A
Cuts at: 822 822
Size: 3791
XcmI CCAnnnn_n'nnnnTGG
Cuts at: 776 1286 776
Size: 510 3281
XhoI C'TCGA_G
Cuts at: 219 219
Size: 3791
XmaI C'CCGG_G
Cuts at: 416 811 416
Size: 395 3396
Enzymes that do cut:
AccI Acc65I AclI AcuI AfeI AflIII AgeI AleI
AlfI AlwNI ApaI ApaLI ApoI AseI AsiSI AvaI
BamHI BanI BanII BbeI BbvCI BciVI BclI BglII
BlpI Bme1580I BmrI BplI BpmI Bpu10I BpuEI BsaI
BsaAI BsaBI BsaHI BsaWI BseYI BsgI BsiEI BsiHKAI
Bsp1286I BspEI BspHI BspMI BsrBI BsrDI BsrFI BssSI
BstAPI BstBI BstXI BstYI BtgI BtgZI BtsI DraI
DrdI EaeI EagI EarI EciI EcoICRI Eco57MI EcoNI
EcoO109I EcoRI FalI FspI HaeII Hin4I HincII KasI
KpnI MluI MmeI MscI MslI MspA1I NaeI NarI
NcoI NgoMIV NspI PciI PflMI PfoI PmeI PpiI
PpuMI PspOMI PspXI PstI PvuI PvuII RsrII SacI
SalI SanDI SapI SbfI ScaI SfcI SfoI SgfI
SmaI SmlI SpeI SphI StyI TaqII TatI TstI
Tth111I XbaI XcmI XhoI XmaI
Enzymes that do not cut:
AarI AatII AflII AhdI AloI AscI AvrII BaeI
BbsI BcgI BfrBI BglI BmgBI BmtI BsaXI BseRI
BsiWI BsmI BsmBI BsrGI BssHII BstEII BstZ17I Bsu36I
ClaI CspCI DraIII EcoRV FseI FspAI HindIII HpaI
MfeI NdeI NheI NotI NruI NsiI PacI PasI
PmlI PshAI PsiI PsrI SacII SexAI SfiI SgrAI
SnaBI SrfI SspI StuI SwaI XmnI ZraI