Restriction map for
pF1KB7589
(Circular) (Six-base) MAPSORT of: pF1KB7589.seq Check: 4610 from: 1 to: 3785
pF1KB7589 3785 bp
With 182 enzymes: SgfI *
June 20, 2008 10:27 ..
AccI GT'mk_AC
Cuts at: 823 823
Size: 3785
Acc65I G'GTAC_C
Cuts at: 801 801
Size: 3785
AclI AA'CG_TT
Cuts at: 3625 3625
Size: 3785
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 1610 2042 2789 3057 1610
Size: 432 747 268 2338
Fragments arranged by size:
2338 747 432 268
AfeI AGC'GCT
Cuts at: 391 391
Size: 3785
AflIII A'CryG_T
Cuts at: 431 1137 2241 3218 431
Size: 706 1104 977 998
Fragments arranged by size:
1104 998 977 706
AgeI A'CCGG_T
Cuts at: 2173 2173
Size: 3785
AleI CACnn'nnGTG
Cuts at: 436 436
Size: 3785
AlwNI CAG_nnn'CTG
Cuts at: 249 537 1090 2657 249
Size: 288 553 1567 1377
Fragments arranged by size:
1567 1377 553 288
ApaI G_GGCC'C
Cuts at: 710 710
Size: 3785
ApaLI G'TGCA_C
Cuts at: 427 2555 427
Size: 2128 1657
ApoI r'AATT_y
Cuts at: 789 3138 3394 789
Size: 2349 256 1180
Fragments arranged by size:
2349 1180 256
AseI AT'TA_AT
Cuts at: 20 20
Size: 3785
AsiSI GCG_AT'CGC
Cuts at: 104 104
Size: 3785
AvaI C'yCGr_G
Cuts at: 496 630 805 496
Size: 134 175 3476
Fragments arranged by size:
3476 175 134
BamHI G'GATC_C
Cuts at: 610 810 610
Size: 200 3585
BanI G'GyrC_C
Cuts at: 111 123 268 717 801 1215 1463 1498 111
Size: 12 145 449 84 414 248 35 2398
Fragments arranged by size:
2398 449 414 248 145 84 35 12
BanII G_rGCy'C
Cuts at: 482 573 710 799 1829 482
Size: 91 137 89 1030 2438
Fragments arranged by size:
2438 1030 137 91 89
BbeI G_GCGC'C
Cuts at: 115 127 272 1219 1467 115
Size: 12 145 947 248 2433
Fragments arranged by size:
2433 947 248 145 12
BbvCI CC'TCA_GC
Cuts at: 701 701
Size: 3785
BciVI GTATCCnnnnn_n'
Cuts at: 1683 2444 3785 1683
Size: 761 1341 1683
Fragments arranged by size:
1683 1341 761
BclI T'GATC_A
Cuts at: 1305 1305
Size: 3785
BglI GCCn_nnn'nGGC
Cuts at: 676 676
Size: 3785
BglII A'GATC_T
Cuts at: 1300 3337 1300
Size: 2037 1748
BlpI GC'TnA_GC
Cuts at: 897 897
Size: 3785
Bme1580I G_kGCm'C
Cuts at: 431 710 722 1410 1503 2559 431
Size: 279 12 688 93 1056 1657
Fragments arranged by size:
1657 1056 688 279 93 12
BmrI ACTGGGnnnn_n'
Cuts at: 997 1078 1413 3557 997
Size: 81 335 2144 1225
Fragments arranged by size:
2144 1225 335 81
BplI GAGnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 710 2152 710
Size: 1442 2343
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 310 328 682 789 310
Size: 18 354 107 3306
Fragments arranged by size:
3306 354 107 18
Bpu10I CC'TnA_GC
Cuts at: 701 701
Size: 3785
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 961 1840 2332 2630 961
Size: 879 492 298 2116
Fragments arranged by size:
2116 879 492 298
BsaI GGTCTCn'nnnn_
Cuts at: 3489 3489
Size: 3785
BsaAI yAC'GTr
Cuts at: 446 1138 446
Size: 692 3093
BsaBI GATnn'nnATC
Cuts at: 6 1299 3336 6
Size: 1293 2037 455
Fragments arranged by size:
2037 1293 455
BsaHI Gr'CG_yC
Cuts at: 112 124 269 548 1216 1464 2166 3662 112
Size: 12 145 279 668 248 702 1496 235
Fragments arranged by size:
1496 702 668 279 248 235 145 12
BsaWI w'CCGG_w
Cuts at: 974 1495 2173 2447 2594 3005 974
Size: 521 678 274 147 411 1754
Fragments arranged by size:
1754 678 521 411 274 147
BsaXI ACnnnnnCTCCnnnnnnn_nnn'
Cuts at: 637 667 637
Size: 30 3755
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 679 679
Size: 3785
BseYI C'CCAG_C
Cuts at: 164 540 622 1210 1968 2545 3041 164
Size: 376 82 588 758 577 496 908
Fragments arranged by size:
908 758 588 577 496 376 82
BsgI GTGCAGnnnnnnnnnnnnnn_nn'
Cuts at: 298 298
Size: 3785
BsiEI CG_ry'CG
Cuts at: 104 1327 1373 2581 104
Size: 1223 46 1208 1308
Fragments arranged by size:
1308 1223 1208 46
BsiHKAI G_wGCw'C
Cuts at: 431 482 655 799 1577 1767 2559 431
Size: 51 173 144 778 190 792 1657
Fragments arranged by size:
1657 792 778 190 173 144 51
BsmBI CGTCTCn'nnnn_
Cuts at: 163 163
Size: 3785
Bsp1286I G_dGCh'C
Cuts at: 431 482 573 655 710 722 799 1410 1503
Size: 51 91 82 55 12 77 611 93
Cuts at: 1503 1577 1767 1829 2559 431
Size: 74 190 62 730 1657
Fragments arranged by size:
1657 730 611 190 93 91 82 77
74 62 55 51 12
BspEI T'CCGG_A
Cuts at: 3005 3005
Size: 3785
BspHI T'CATG_A
Cuts at: 2961 2961
Size: 3785
BspMI ACCTGCnnnn'nnnn_
Cuts at: 835 1049 1351 1732 835
Size: 214 302 381 2888
Fragments arranged by size:
2888 381 302 214
BsrBI CCG'CTC
Cuts at: 50 2077 2131 3615 3780 50
Size: 2027 54 1484 165 55
Fragments arranged by size:
2027 1484 165 55 54
BsrDI GCAATG_nn'
Cuts at: 1697 1697
Size: 3785
BsrFI r'CCGG_y
Cuts at: 400 1783 1964 2173 400
Size: 1383 181 209 2012
Fragments arranged by size:
2012 1383 209 181
BssHII G'CGCG_C
Cuts at: 219 221 352 219
Size: 2 131 3652
Fragments arranged by size:
3652 131 2
BssSI C'ACGA_G
Cuts at: 421 2056 2414 421
Size: 1635 358 1792
Fragments arranged by size:
1792 1635 358
BstAPI GCAn_nnn'nTGC
Cuts at: 249 426 1162 249
Size: 177 736 2872
Fragments arranged by size:
2872 736 177
BstBI TT'CG_AA
Cuts at: 2146 2146
Size: 3785
BstYI r'GATC_y
Cuts at: 1 534 610 810 1277 1300 1635 1881 2893
Size: 533 76 200 467 23 335 246 1012
Cuts at: 2893 2979 2991 3337 1
Size: 86 12 346 449
Fragments arranged by size:
1012 533 467 449 346 335 246 200
86 76 23 12
Bsu36I CC'TnA_GG
Cuts at: 674 674
Size: 3785
BtgI C'CryG_G
Cuts at: 107 119 107
Size: 12 3773
BtgZI GCGATGnnnnnnnnnn'nnnn_
Cuts at: 1735 1916 2079 1735
Size: 181 163 3441
Fragments arranged by size:
3441 181 163
BtsI GCAGTG_nn'
Cuts at: 1028 1028
Size: 3785
DraI TTT'AAA
Cuts at: 784 784
Size: 3785
DraIII CAC_nnn'GTG
Cuts at: 426 426
Size: 3785
DrdI GACnn_nn'nnGTC
Cuts at: 466 1491 2349 466
Size: 1025 858 1902
Fragments arranged by size:
1902 1025 858
EaeI y'GGCC_r
Cuts at: 129 1370 1544 1935 1962 3307 129
Size: 1241 174 391 27 1345 607
Fragments arranged by size:
1345 1241 607 391 174 27
EagI C'GGCC_G
Cuts at: 1370 1370
Size: 3785
EarI CTCTTCn'nnn_
Cuts at: 1808 2018 1808
Size: 210 3575
EciI GGCGGAnnnnnnnnn_nn'
Cuts at: 391 1993 2303 2449 3595 391
Size: 1602 310 146 1146 581
Fragments arranged by size:
1602 1146 581 310 146
EcoICRI GAG'CTC
Cuts at: 480 797 480
Size: 317 3468
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 310 328 682 789 1610 2042 2789 3057 310
Size: 18 354 107 821 432 747 268 1038
Fragments arranged by size:
1038 821 747 432 354 268 107 18
EcoNI CCTnn'n_nnAGG
Cuts at: 664 664
Size: 3785
EcoO109I rG'GnC_Cy
Cuts at: 634 669 706 924 634
Size: 35 37 218 3495
Fragments arranged by size:
3495 218 37 35
EcoRI G'AATT_C
Cuts at: 789 789
Size: 3785
FalI AAGnnnnnCTTnnnnnnnn_nnnnn'
Cuts at: 1804 1804
Size: 3785
FspI TGC'GCA
Cuts at: 1566 1566
Size: 3785
HaeII r_GCGC'y
Cuts at: 115 127 272 393 605 1219 1467 2489 3468
Size: 12 145 121 212 614 248 1022 979
Cuts at: 3468 115
Size: 432
Fragments arranged by size:
1022 979 614 432 248 212 145 121
12
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 637 669 1627 1657 1659 1689 637
Size: 32 958 30 2 30 2733
Fragments arranged by size:
2733 958 32 30 30 2
HincII GTy'rAC
Cuts at: 824 824
Size: 3785
KasI G'GCGC_C
Cuts at: 111 123 268 1215 1463 111
Size: 12 145 947 248 2433
Fragments arranged by size:
2433 947 248 145 12
KpnI G_GTAC'C
Cuts at: 805 805
Size: 3785
MluI A'CGCG_T
Cuts at: 3218 3218
Size: 3785
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 680 2456 2640 680
Size: 1776 184 1825
Fragments arranged by size:
1825 1776 184
MscI TGG'CCA
Cuts at: 1546 1546
Size: 3785
MslI CAynn'nnrTG
Cuts at: 436 1774 1901 436
Size: 1338 127 2320
Fragments arranged by size:
2320 1338 127
MspA1I CmG'CkG
Cuts at: 121 369 510 896 1210 1570 2583 2828 121
Size: 248 141 386 314 360 1013 245 1078
Fragments arranged by size:
1078 1013 386 360 314 248 245 141
NaeI GCC'GGC
Cuts at: 402 1966 402
Size: 1564 2221
NarI GG'CG_CC
Cuts at: 112 124 269 1216 1464 112
Size: 12 145 947 248 2433
Fragments arranged by size:
2433 947 248 145 12
NcoI C'CATG_G
Cuts at: 107 107
Size: 3785
NgoMIV G'CCGG_C
Cuts at: 400 1964 400
Size: 1564 2221
NspI r_CATG'y
Cuts at: 420 838 1338 2245 420
Size: 418 500 907 1960
Fragments arranged by size:
1960 907 500 418
PasI CC'CwG_GG
Cuts at: 729 729
Size: 3785
PciI A'CATG_T
Cuts at: 2241 2241
Size: 3785
PfoI T'CCnGG_A
Cuts at: 530 606 530
Size: 76 3709
PmeI GTTT'AAAC
Cuts at: 784 784
Size: 3785
PmlI CAC'GTG
Cuts at: 446 446
Size: 3785
PpiI GAACnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 2924 2956 2924
Size: 32 3753
PpuMI rG'GwC_Cy
Cuts at: 634 634
Size: 3785
PspOMI G'GGCC_C
Cuts at: 706 706
Size: 3785
PspXI vC'TCGA_Gb
Cuts at: 496 496
Size: 3785
PstI C_TGCA'G
Cuts at: 245 374 832 1517 245
Size: 129 458 685 2513
Fragments arranged by size:
2513 685 458 129
PvuI CG_AT'CG
Cuts at: 104 104
Size: 3785
PvuII CAG'CTG
Cuts at: 369 1210 1570 369
Size: 841 360 2584
Fragments arranged by size:
2584 841 360
RsrII CG'GwC_CG
Cuts at: 1980 1980
Size: 3785
SacI G_AGCT'C
Cuts at: 482 799 482
Size: 317 3468
SacII CC_GC'GG
Cuts at: 122 122
Size: 3785
SalI G'TCGA_C
Cuts at: 822 822
Size: 3785
SapI GCTCTTCn'nnn_
Cuts at: 1808 2018 1808
Size: 210 3575
SbfI CC_TGCA'GG
Cuts at: 245 832 245
Size: 587 3198
ScaI AGT'ACT
Cuts at: 3302 3302
Size: 3785
SfcI C'TryA_G
Cuts at: 33 241 370 828 1513 2506 2697 33
Size: 208 129 458 685 993 191 1121
Fragments arranged by size:
1121 993 685 458 208 191 129
SfoI GGC'GCC
Cuts at: 113 125 270 1217 1465 113
Size: 12 145 947 248 2433
Fragments arranged by size:
2433 947 248 145 12
SgfI GCG_AT'CGC
Cuts at: 104 104
Size: 3785
SmaI CCC'GGG
Cuts at: 632 807 632
Size: 175 3610
SmlI C'TyrA_G
Cuts at: 496 940 1855 2347 2609 496
Size: 444 915 492 262 1672
Fragments arranged by size:
1672 915 492 444 262
SpeI A'CTAG_T
Cuts at: 67 67
Size: 3785
SphI G_CATG'C
Cuts at: 420 838 1338 420
Size: 418 500 2867
Fragments arranged by size:
2867 500 418
StuI AGG'CCT
Cuts at: 774 774
Size: 3785
StyI C'CwwG_G
Cuts at: 107 775 919 1273 107
Size: 668 144 354 2619
Fragments arranged by size:
2619 668 354 144
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 1368 1501 1960 2169 1368
Size: 133 459 209 2984
Fragments arranged by size:
2984 459 209 133
TatI w'GTAC_w
Cuts at: 3300 3300
Size: 3785
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 634 666 3302 3334 634
Size: 32 2636 32 1085
Fragments arranged by size:
2636 1085 32 32
Tth111I GACn'n_nGTC
Cuts at: 1582 1582
Size: 3785
XbaI T'CTAG_A
Cuts at: 816 816
Size: 3785
XcmI CCAnnnn_n'nnnnTGG
Cuts at: 1280 1280
Size: 3785
XhoI C'TCGA_G
Cuts at: 496 496
Size: 3785
XmaI C'CCGG_G
Cuts at: 630 805 630
Size: 175 3610
Enzymes that do cut:
AccI Acc65I AclI AcuI AfeI AflIII AgeI AleI
AlwNI ApaI ApaLI ApoI AseI AsiSI AvaI BamHI
BanI BanII BbeI BbvCI BciVI BclI BglI BglII
BlpI Bme1580I BmrI BplI BpmI Bpu10I BpuEI BsaI
BsaAI BsaBI BsaHI BsaWI BsaXI BseRI BseYI BsgI
BsiEI BsiHKAI BsmBI Bsp1286I BspEI BspHI BspMI BsrBI
BsrDI BsrFI BssHII BssSI BstAPI BstBI BstYI Bsu36I
BtgI BtgZI BtsI DraI DraIII DrdI EaeI EagI
EarI EciI EcoICRI Eco57MI EcoNI EcoO109I EcoRI FalI
FspI HaeII Hin4I HincII KasI KpnI MluI MmeI
MscI MslI MspA1I NaeI NarI NcoI NgoMIV NspI
PasI PciI PfoI PmeI PmlI PpiI PpuMI PspOMI
PspXI PstI PvuI PvuII RsrII SacI SacII SalI
SapI SbfI ScaI SfcI SfoI SgfI SmaI SmlI
SpeI SphI StuI StyI TaqII TatI TstI Tth111I
XbaI XcmI XhoI XmaI
Enzymes that do not cut:
AarI AatII AflII AhdI AlfI AloI AscI AvrII
BaeI BbsI BcgI BfrBI BmgBI BmtI BsiWI BsmI
BsrGI BstEII BstXI BstZ17I ClaI CspCI EcoRV FseI
FspAI HindIII HpaI MfeI NdeI NheI NotI NruI
NsiI PacI PflMI PshAI PsiI PsrI SanDI SexAI
SfiI SgrAI SnaBI SrfI SspI SwaI XmnI ZraI