Restriction map for
pF1KB7588
(Circular) (Six-base) MAPSORT of: pF1KB7588.seq Check: 4278 from: 1 to: 3980
pF1KB7588 3980 bp
With 182 enzymes: SgfI *
June 20, 2008 10:27 ..
AarI CACCTGCnnnn'nnnn_
Cuts at: 926 926
Size: 3980
AatII G_ACGT'C
Cuts at: 178 178
Size: 3980
AccI GT'mk_AC
Cuts at: 1018 1018
Size: 3980
Acc65I G'GTAC_C
Cuts at: 996 996
Size: 3980
AclI AA'CG_TT
Cuts at: 3820 3820
Size: 3980
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 212 1805 2237 2984 3252 212
Size: 1593 432 747 268 940
Fragments arranged by size:
1593 940 747 432 268
AflIII A'CryG_T
Cuts at: 1332 2436 3413 1332
Size: 1104 977 1899
Fragments arranged by size:
1899 1104 977
AgeI A'CCGG_T
Cuts at: 2368 2368
Size: 3980
AloI GAACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 658 690 862 894 658
Size: 32 172 32 3744
Fragments arranged by size:
3744 172 32 32
AlwNI CAG_nnn'CTG
Cuts at: 1285 2852 1285
Size: 1567 2413
ApaLI G'TGCA_C
Cuts at: 2750 2750
Size: 3980
ApoI r'AATT_y
Cuts at: 984 3333 3589 984
Size: 2349 256 1375
Fragments arranged by size:
2349 1375 256
AseI AT'TA_AT
Cuts at: 20 20
Size: 3980
AsiSI GCG_AT'CGC
Cuts at: 104 104
Size: 3980
AvaI C'yCGr_G
Cuts at: 220 481 1000 220
Size: 261 519 3200
Fragments arranged by size:
3200 519 261
BaeI ACnnnnGTAyCnnnnnnn_nnnnn'
Cuts at: 876 909 876
Size: 33 3947
BamHI G'GATC_C
Cuts at: 1005 1005
Size: 3980
BanI G'GyrC_C
Cuts at: 424 996 1410 1658 1693 424
Size: 572 414 248 35 2711
Fragments arranged by size:
2711 572 414 248 35
BanII G_rGCy'C
Cuts at: 270 482 785 793 934 994 2024 270
Size: 212 303 8 141 60 1030 2226
Fragments arranged by size:
2226 1030 303 212 141 60 8
BbeI G_GCGC'C
Cuts at: 428 1414 1662 428
Size: 986 248 2746
Fragments arranged by size:
2746 986 248
BbsI GAAGACnn'nnnn_
Cuts at: 218 586 218
Size: 368 3612
BciVI GTATCCnnnnn_n'
Cuts at: 1878 2639 3980 1878
Size: 761 1341 1878
Fragments arranged by size:
1878 1341 761
BclI T'GATC_A
Cuts at: 1500 1500
Size: 3980
BglI GCCn_nnn'nGGC
Cuts at: 616 616
Size: 3980
BglII A'GATC_T
Cuts at: 1495 3532 1495
Size: 2037 1943
BlpI GC'TnA_GC
Cuts at: 805 1092 805
Size: 287 3693
Bme1580I G_kGCm'C
Cuts at: 1605 1698 2754 1605
Size: 93 1056 2831
Fragments arranged by size:
2831 1056 93
BmrI ACTGGGnnnn_n'
Cuts at: 1192 1273 1608 3752 1192
Size: 81 335 2144 1420
Fragments arranged by size:
2144 1420 335 81
BmtI G_CTAG'C
Cuts at: 404 404
Size: 3980
BplI GAGnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 2347 2347
Size: 3980
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 717 752 886 717
Size: 35 134 3811
Fragments arranged by size:
3811 134 35
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 1156 2035 2527 2825 1156
Size: 879 492 298 2311
Fragments arranged by size:
2311 879 492 298
BsaI GGTCTCn'nnnn_
Cuts at: 3684 3684
Size: 3980
BsaAI yAC'GTr
Cuts at: 1333 1333
Size: 3980
BsaBI GATnn'nnATC
Cuts at: 6 1494 3531 6
Size: 1488 2037 455
Fragments arranged by size:
2037 1488 455
BsaHI Gr'CG_yC
Cuts at: 175 425 1411 1659 2361 3857 175
Size: 250 986 248 702 1496 298
Fragments arranged by size:
1496 986 702 298 250 248
BsaWI w'CCGG_w
Cuts at: 1169 1690 2368 2642 2789 3200 1169
Size: 521 678 274 147 411 1949
Fragments arranged by size:
1949 678 521 411 274 147
BsaXI ACnnnnnCTCCnnnnnnn_nnn'
Cuts at: 388 418 388
Size: 30 3950
BseYI C'CCAG_C
Cuts at: 136 369 627 727 849 1405 2163 2740 3236
Size: 233 258 100 122 556 758 577 496
Cuts at: 3236 136
Size: 880
Fragments arranged by size:
880 758 577 556 496 258 233 122
100
BsgI GTGCAGnnnnnnnnnnnnnn_nn'
Cuts at: 914 914
Size: 3980
BsiEI CG_ry'CG
Cuts at: 104 1522 1568 2776 104
Size: 1418 46 1208 1308
Fragments arranged by size:
1418 1308 1208 46
BsiHKAI G_wGCw'C
Cuts at: 722 994 1772 1962 2754 722
Size: 272 778 190 792 1948
Fragments arranged by size:
1948 792 778 272 190
BsmBI CGTCTCn'nnnn_
Cuts at: 203 203
Size: 3980
Bsp1286I G_dGCh'C
Cuts at: 270 482 722 785 793 934 994 1605 1698
Size: 212 240 63 8 141 60 611 93
Cuts at: 1698 1772 1962 2024 2754 270
Size: 74 190 62 730 1496
Fragments arranged by size:
1496 730 611 240 212 190 141 93
74 63 62 60 8
BspEI T'CCGG_A
Cuts at: 3200 3200
Size: 3980
BspHI T'CATG_A
Cuts at: 692 3156 692
Size: 2464 1516
BspMI ACCTGCnnnn'nnnn_
Cuts at: 926 1030 1244 1546 1927 926
Size: 104 214 302 381 2979
Fragments arranged by size:
2979 381 302 214 104
BsrBI CCG'CTC
Cuts at: 50 800 2272 2326 3810 3975 50
Size: 750 1472 54 1484 165 55
Fragments arranged by size:
1484 1472 750 165 55 54
BsrDI GCAATG_nn'
Cuts at: 1892 1892
Size: 3980
BsrFI r'CCGG_y
Cuts at: 1978 2159 2368 1978
Size: 181 209 3590
Fragments arranged by size:
3590 209 181
BssSI C'ACGA_G
Cuts at: 2251 2609 2251
Size: 358 3622
BstAPI GCAn_nnn'nTGC
Cuts at: 1357 1357
Size: 3980
BstBI TT'CG_AA
Cuts at: 2341 2341
Size: 3980
BstXI CCAn_nnnn'nTGG
Cuts at: 776 864 776
Size: 88 3892
BstYI r'GATC_y
Cuts at: 1 673 1005 1472 1495 1830 2076 3088 3174
Size: 672 332 467 23 335 246 1012 86
Cuts at: 3174 3186 3532 1
Size: 12 346 449
Fragments arranged by size:
1012 672 467 449 346 335 332 246
86 23 12
BtgZI GCGATGnnnnnnnnnn'nnnn_
Cuts at: 1930 2111 2274 1930
Size: 181 163 3636
Fragments arranged by size:
3636 181 163
BtsI GCAGTG_nn'
Cuts at: 1223 1223
Size: 3980
DraI TTT'AAA
Cuts at: 979 979
Size: 3980
DraIII CAC_nnn'GTG
Cuts at: 345 345
Size: 3980
DrdI GACnn_nn'nnGTC
Cuts at: 130 1686 2544 130
Size: 1556 858 1566
Fragments arranged by size:
1566 1556 858
EaeI y'GGCC_r
Cuts at: 554 617 1565 1739 2130 2157 3502 554
Size: 63 948 174 391 27 1345 1032
Fragments arranged by size:
1345 1032 948 391 174 63 27
EagI C'GGCC_G
Cuts at: 1565 1565
Size: 3980
EarI CTCTTCn'nnn_
Cuts at: 2003 2213 2003
Size: 210 3770
EciI GGCGGAnnnnnnnnn_nn'
Cuts at: 345 2188 2498 2644 3790 345
Size: 1843 310 146 1146 535
Fragments arranged by size:
1843 1146 535 310 146
EcoICRI GAG'CTC
Cuts at: 992 992
Size: 3980
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 212 717 752 886 1805 2237 2984 3252 212
Size: 505 35 134 919 432 747 268 940
Fragments arranged by size:
940 919 747 505 432 268 134 35
EcoO109I rG'GnC_Cy
Cuts at: 132 1119 132
Size: 987 2993
EcoRI G'AATT_C
Cuts at: 984 984
Size: 3980
FalI AAGnnnnnCTTnnnnnnnn_nnnnn'
Cuts at: 1999 1999
Size: 3980
FspI TGC'GCA
Cuts at: 1761 1761
Size: 3980
HaeII r_GCGC'y
Cuts at: 428 449 955 1414 1662 2684 3663 428
Size: 21 506 459 248 1022 979 745
Fragments arranged by size:
1022 979 745 506 459 248 21
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 665 697 1822 1852 1854 1884 665
Size: 32 1125 30 2 30 2761
Fragments arranged by size:
2761 1125 32 30 30 2
HincII GTy'rAC
Cuts at: 1019 1019
Size: 3980
HindIII A'AGCT_T
Cuts at: 377 377
Size: 3980
KasI G'GCGC_C
Cuts at: 424 1410 1658 424
Size: 986 248 2746
Fragments arranged by size:
2746 986 248
KpnI G_GTAC'C
Cuts at: 1000 1000
Size: 3980
MluI A'CGCG_T
Cuts at: 3413 3413
Size: 3980
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 856 863 2651 2835 856
Size: 7 1788 184 2001
Fragments arranged by size:
2001 1788 184 7
MscI TGG'CCA
Cuts at: 1741 1741
Size: 3980
MslI CAynn'nnrTG
Cuts at: 818 1969 2096 818
Size: 1151 127 2702
Fragments arranged by size:
2702 1151 127
MspA1I CmG'CkG
Cuts at: 552 772 948 1091 1405 1765 2778 3023 552
Size: 220 176 143 314 360 1013 245 1509
Fragments arranged by size:
1509 1013 360 314 245 220 176 143
NaeI GCC'GGC
Cuts at: 2161 2161
Size: 3980
NarI GG'CG_CC
Cuts at: 425 1411 1659 425
Size: 986 248 2746
Fragments arranged by size:
2746 986 248
NgoMIV G'CCGG_C
Cuts at: 2159 2159
Size: 3980
NheI G'CTAG_C
Cuts at: 400 400
Size: 3980
NspI r_CATG'y
Cuts at: 1033 1533 2440 1033
Size: 500 907 2573
Fragments arranged by size:
2573 907 500
PasI CC'CwG_GG
Cuts at: 786 786
Size: 3980
PciI A'CATG_T
Cuts at: 2436 2436
Size: 3980
PfoI T'CCnGG_A
Cuts at: 961 961
Size: 3980
PmeI GTTT'AAAC
Cuts at: 979 979
Size: 3980
PpiI GAACnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 3119 3151 3119
Size: 32 3948
PpuMI rG'GwC_Cy
Cuts at: 132 132
Size: 3980
PstI C_TGCA'G
Cuts at: 1027 1712 1027
Size: 685 3295
PvuI CG_AT'CG
Cuts at: 104 104
Size: 3980
PvuII CAG'CTG
Cuts at: 552 1405 1765 552
Size: 853 360 2767
Fragments arranged by size:
2767 853 360
RsrII CG'GwC_CG
Cuts at: 2175 2175
Size: 3980
SacI G_AGCT'C
Cuts at: 994 994
Size: 3980
SalI G'TCGA_C
Cuts at: 1017 1017
Size: 3980
SanDI GG'GwC_CC
Cuts at: 132 132
Size: 3980
SapI GCTCTTCn'nnn_
Cuts at: 2003 2213 2003
Size: 210 3770
SbfI CC_TGCA'GG
Cuts at: 1027 1027
Size: 3980
ScaI AGT'ACT
Cuts at: 3497 3497
Size: 3980
SfcI C'TryA_G
Cuts at: 33 249 390 1023 1708 2701 2892 33
Size: 216 141 633 685 993 191 1121
Fragments arranged by size:
1121 993 685 633 216 191 141
SfoI GGC'GCC
Cuts at: 426 1412 1660 426
Size: 986 248 2746
Fragments arranged by size:
2746 986 248
SgfI GCG_AT'CGC
Cuts at: 104 104
Size: 3980
SmaI CCC'GGG
Cuts at: 1002 1002
Size: 3980
SmlI C'TyrA_G
Cuts at: 1135 2050 2542 2804 1135
Size: 915 492 262 2311
Fragments arranged by size:
2311 915 492 262
SpeI A'CTAG_T
Cuts at: 67 67
Size: 3980
SphI G_CATG'C
Cuts at: 1033 1533 1033
Size: 500 3480
StyI C'CwwG_G
Cuts at: 1114 1468 1114
Size: 354 3626
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 490 1563 1696 2155 2364 490
Size: 1073 133 459 209 2106
Fragments arranged by size:
2106 1073 459 209 133
TatI w'GTAC_w
Cuts at: 3495 3495
Size: 3980
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 3497 3529 3497
Size: 32 3948
Tth111I GACn'n_nGTC
Cuts at: 1777 1777
Size: 3980
XbaI T'CTAG_A
Cuts at: 1011 1011
Size: 3980
XcmI CCAnnnn_n'nnnnTGG
Cuts at: 549 741 1475 549
Size: 192 734 3054
Fragments arranged by size:
3054 734 192
XmaI C'CCGG_G
Cuts at: 1000 1000
Size: 3980
ZraI GAC'GTC
Cuts at: 176 176
Size: 3980
Enzymes that do cut:
AarI AatII AccI Acc65I AclI AcuI AflIII AgeI
AloI AlwNI ApaLI ApoI AseI AsiSI AvaI BaeI
BamHI BanI BanII BbeI BbsI BciVI BclI BglI
BglII BlpI Bme1580I BmrI BmtI BplI BpmI BpuEI
BsaI BsaAI BsaBI BsaHI BsaWI BsaXI BseYI BsgI
BsiEI BsiHKAI BsmBI Bsp1286I BspEI BspHI BspMI BsrBI
BsrDI BsrFI BssSI BstAPI BstBI BstXI BstYI BtgZI
BtsI DraI DraIII DrdI EaeI EagI EarI EciI
EcoICRI Eco57MI EcoO109I EcoRI FalI FspI HaeII Hin4I
HincII HindIII KasI KpnI MluI MmeI MscI MslI
MspA1I NaeI NarI NgoMIV NheI NspI PasI PciI
PfoI PmeI PpiI PpuMI PstI PvuI PvuII RsrII
SacI SalI SanDI SapI SbfI ScaI SfcI SfoI
SgfI SmaI SmlI SpeI SphI StyI TaqII TatI
TstI Tth111I XbaI XcmI XmaI ZraI
Enzymes that do not cut:
AfeI AflII AhdI AleI AlfI ApaI AscI AvrII
BbvCI BcgI BfrBI BmgBI Bpu10I BseRI BsiWI BsmI
BsrGI BssHII BstEII BstZ17I Bsu36I BtgI ClaI CspCI
EcoNI EcoRV FseI FspAI HpaI MfeI NcoI NdeI
NotI NruI NsiI PacI PflMI PmlI PshAI PsiI
PspOMI PspXI PsrI SacII SexAI SfiI SgrAI SnaBI
SrfI SspI StuI SwaI XhoI XmnI