Restriction map for
pF1KB7586
(Circular) (Six-base) MAPSORT of: pF1KB7586.seq Check: 7742 from: 1 to: 3977
pF1KB7586 3977 bp
With 182 enzymes: SgfI *
June 20, 2008 10:27 ..
AccI GT'mk_AC
Cuts at: 164 1015 164
Size: 851 3126
Acc65I G'GTAC_C
Cuts at: 993 993
Size: 3977
AclI AA'CG_TT
Cuts at: 416 3817 416
Size: 3401 576
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 236 1802 2234 2981 3249 236
Size: 1566 432 747 268 964
Fragments arranged by size:
1566 964 747 432 268
AflIII A'CryG_T
Cuts at: 1329 2433 3410 1329
Size: 1104 977 1896
Fragments arranged by size:
1896 1104 977
AgeI A'CCGG_T
Cuts at: 2365 2365
Size: 3977
AlwNI CAG_nnn'CTG
Cuts at: 552 1282 2849 552
Size: 730 1567 1680
Fragments arranged by size:
1680 1567 730
ApaLI G'TGCA_C
Cuts at: 2747 2747
Size: 3977
ApoI r'AATT_y
Cuts at: 919 981 3330 3586 919
Size: 62 2349 256 1310
Fragments arranged by size:
2349 1310 256 62
AseI AT'TA_AT
Cuts at: 20 694 20
Size: 674 3303
AsiSI GCG_AT'CGC
Cuts at: 104 104
Size: 3977
AvaI C'yCGr_G
Cuts at: 388 997 388
Size: 609 3368
BamHI G'GATC_C
Cuts at: 1002 1002
Size: 3977
BanI G'GyrC_C
Cuts at: 352 394 475 768 993 1407 1655 1690 352
Size: 42 81 293 225 414 248 35 2639
Fragments arranged by size:
2639 414 293 248 225 81 42 35
BanII G_rGCy'C
Cuts at: 303 991 2021 303
Size: 688 1030 2259
Fragments arranged by size:
2259 1030 688
BbeI G_GCGC'C
Cuts at: 356 479 1411 1659 356
Size: 123 932 248 2674
Fragments arranged by size:
2674 932 248 123
BbvCI CC'TCA_GC
Cuts at: 755 755
Size: 3977
BciVI GTATCCnnnnn_n'
Cuts at: 1875 2636 3977 1875
Size: 761 1341 1875
Fragments arranged by size:
1875 1341 761
BclI T'GATC_A
Cuts at: 1497 1497
Size: 3977
BglI GCCn_nnn'nGGC
Cuts at: 473 766 473
Size: 293 3684
BglII A'GATC_T
Cuts at: 1492 3529 1492
Size: 2037 1940
BlpI GC'TnA_GC
Cuts at: 151 1089 151
Size: 938 3039
Bme1580I G_kGCm'C
Cuts at: 397 1602 1695 2751 397
Size: 1205 93 1056 1623
Fragments arranged by size:
1623 1205 1056 93
BmrI ACTGGGnnnn_n'
Cuts at: 578 1189 1270 1605 3749 578
Size: 611 81 335 2144 806
Fragments arranged by size:
2144 806 611 335 81
BplI GAGnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 2344 2344
Size: 3977
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 511 574 511
Size: 63 3914
Bpu10I CC'TnA_GC
Cuts at: 251 257 755 251
Size: 6 498 3473
Fragments arranged by size:
3473 498 6
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 1153 2032 2524 2822 1153
Size: 879 492 298 2308
Fragments arranged by size:
2308 879 492 298
BsaI GGTCTCn'nnnn_
Cuts at: 377 3681 377
Size: 3304 673
BsaAI yAC'GTr
Cuts at: 1330 1330
Size: 3977
BsaBI GATnn'nnATC
Cuts at: 6 1491 3528 6
Size: 1485 2037 455
Fragments arranged by size:
2037 1485 455
BsaHI Gr'CG_yC
Cuts at: 353 476 1408 1656 2358 3854 353
Size: 123 932 248 702 1496 476
Fragments arranged by size:
1496 932 702 476 248 123
BsaWI w'CCGG_w
Cuts at: 117 1166 1687 2365 2639 2786 3197 117
Size: 1049 521 678 274 147 411 897
Fragments arranged by size:
1049 897 678 521 411 274 147
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 244 690 848 244
Size: 446 158 3373
Fragments arranged by size:
3373 446 158
BseYI C'CCAG_C
Cuts at: 1402 2160 2737 3233 1402
Size: 758 577 496 2146
Fragments arranged by size:
2146 758 577 496
BsgI GTGCAGnnnnnnnnnnnnnn_nn'
Cuts at: 661 661
Size: 3977
BsiEI CG_ry'CG
Cuts at: 104 1519 1565 2773 104
Size: 1415 46 1208 1308
Fragments arranged by size:
1415 1308 1208 46
BsiHKAI G_wGCw'C
Cuts at: 991 1769 1959 2751 991
Size: 778 190 792 2217
Fragments arranged by size:
2217 792 778 190
BsmI GAATG_Cn'
Cuts at: 198 198
Size: 3977
Bsp1286I G_dGCh'C
Cuts at: 303 397 991 1602 1695 1769 1959 2021 2751
Size: 94 594 611 93 74 190 62 730
Cuts at: 2751 303
Size: 1529
Fragments arranged by size:
1529 730 611 594 190 94 93 74
62
BspEI T'CCGG_A
Cuts at: 117 3197 117
Size: 3080 897
BspHI T'CATG_A
Cuts at: 3153 3153
Size: 3977
BspMI ACCTGCnnnn'nnnn_
Cuts at: 1027 1241 1543 1924 1027
Size: 214 302 381 3080
Fragments arranged by size:
3080 381 302 214
BsrBI CCG'CTC
Cuts at: 50 2269 2323 3807 3972 50
Size: 2219 54 1484 165 55
Fragments arranged by size:
2219 1484 165 55 54
BsrDI GCAATG_nn'
Cuts at: 535 1889 535
Size: 1354 2623
BsrFI r'CCGG_y
Cuts at: 1975 2156 2365 1975
Size: 181 209 3587
Fragments arranged by size:
3587 209 181
BssSI C'ACGA_G
Cuts at: 2248 2606 2248
Size: 358 3619
BstAPI GCAn_nnn'nTGC
Cuts at: 1354 1354
Size: 3977
BstBI TT'CG_AA
Cuts at: 2338 2338
Size: 3977
BstEII G'GTnAC_C
Cuts at: 744 744
Size: 3977
BstYI r'GATC_y
Cuts at: 1 1002 1469 1492 1827 2073 3085 3171 3183
Size: 1001 467 23 335 246 1012 86 12
Cuts at: 3183 3529 1
Size: 346 449
Fragments arranged by size:
1012 1001 467 449 346 335 246 86
23 12
BtgI C'CryG_G
Cuts at: 107 107
Size: 3977
BtgZI GCGATGnnnnnnnnnn'nnnn_
Cuts at: 422 1927 2108 2271 422
Size: 1505 181 163 2128
Fragments arranged by size:
2128 1505 181 163
BtsI GCAGTG_nn'
Cuts at: 644 731 1220 644
Size: 87 489 3401
Fragments arranged by size:
3401 489 87
DraI TTT'AAA
Cuts at: 976 976
Size: 3977
DrdI GACnn_nn'nnGTC
Cuts at: 1683 2541 1683
Size: 858 3119
EaeI y'GGCC_r
Cuts at: 366 909 1562 1736 2127 2154 3499 366
Size: 543 653 174 391 27 1345 844
Fragments arranged by size:
1345 844 653 543 391 174 27
EagI C'GGCC_G
Cuts at: 1562 1562
Size: 3977
EarI CTCTTCn'nnn_
Cuts at: 212 230 657 2000 2210 212
Size: 18 427 1343 210 1979
Fragments arranged by size:
1979 1343 427 210 18
EciI GGCGGAnnnnnnnnn_nn'
Cuts at: 323 344 2185 2495 2641 3787 323
Size: 21 1841 310 146 1146 513
Fragments arranged by size:
1841 1146 513 310 146 21
EcoICRI GAG'CTC
Cuts at: 989 989
Size: 3977
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 236 511 574 1802 2234 2981 3249 236
Size: 275 63 1228 432 747 268 964
Fragments arranged by size:
1228 964 747 432 275 268 63
EcoNI CCTnn'n_nnAGG
Cuts at: 260 260
Size: 3977
EcoO109I rG'GnC_Cy
Cuts at: 1116 1116
Size: 3977
EcoRI G'AATT_C
Cuts at: 981 981
Size: 3977
FalI AAGnnnnnCTTnnnnnnnn_nnnnn'
Cuts at: 1996 1996
Size: 3977
FspI TGC'GCA
Cuts at: 1758 1758
Size: 3977
HaeII r_GCGC'y
Cuts at: 356 479 1411 1659 2681 3660 356
Size: 123 932 248 1022 979 673
Fragments arranged by size:
1022 979 932 673 248 123
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 666 698 1819 1849 1851 1881 666
Size: 32 1121 30 2 30 2762
Fragments arranged by size:
2762 1121 32 30 30 2
HincII GTy'rAC
Cuts at: 165 1016 165
Size: 851 3126
KasI G'GCGC_C
Cuts at: 352 475 1407 1655 352
Size: 123 932 248 2674
Fragments arranged by size:
2674 932 248 123
KpnI G_GTAC'C
Cuts at: 997 997
Size: 3977
MluI A'CGCG_T
Cuts at: 3410 3410
Size: 3977
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 591 2648 2832 591
Size: 2057 184 1736
Fragments arranged by size:
2057 1736 184
MscI TGG'CCA
Cuts at: 368 911 1738 368
Size: 543 827 2607
Fragments arranged by size:
2607 827 543
MslI CAynn'nnrTG
Cuts at: 622 724 1966 2093 622
Size: 102 1242 127 2506
Fragments arranged by size:
2506 1242 127 102
MspA1I CmG'CkG
Cuts at: 401 543 754 785 907 1088 1402 1762 2775
Size: 142 211 31 122 181 314 360 1013
Cuts at: 2775 3020 401
Size: 245 1358
Fragments arranged by size:
1358 1013 360 314 245 211 181 142
122 31
NaeI GCC'GGC
Cuts at: 2158 2158
Size: 3977
NarI GG'CG_CC
Cuts at: 353 476 1408 1656 353
Size: 123 932 248 2674
Fragments arranged by size:
2674 932 248 123
NcoI C'CATG_G
Cuts at: 107 107
Size: 3977
NgoMIV G'CCGG_C
Cuts at: 2156 2156
Size: 3977
NspI r_CATG'y
Cuts at: 711 1030 1530 2437 711
Size: 319 500 907 2251
Fragments arranged by size:
2251 907 500 319
PciI A'CATG_T
Cuts at: 2433 2433
Size: 3977
PflMI CCAn_nnn'nTGG
Cuts at: 907 907
Size: 3977
PmeI GTTT'AAAC
Cuts at: 976 976
Size: 3977
PpiI GAACnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 3116 3148 3116
Size: 32 3945
PspXI vC'TCGA_Gb
Cuts at: 388 388
Size: 3977
PstI C_TGCA'G
Cuts at: 162 548 1024 1709 162
Size: 386 476 685 2430
Fragments arranged by size:
2430 685 476 386
PvuI CG_AT'CG
Cuts at: 104 104
Size: 3977
PvuII CAG'CTG
Cuts at: 543 754 1402 1762 543
Size: 211 648 360 2758
Fragments arranged by size:
2758 648 360 211
RsrII CG'GwC_CG
Cuts at: 2172 2172
Size: 3977
SacI G_AGCT'C
Cuts at: 991 991
Size: 3977
SalI G'TCGA_C
Cuts at: 163 1014 163
Size: 851 3126
SapI GCTCTTCn'nnn_
Cuts at: 657 2000 2210 657
Size: 1343 210 2424
Fragments arranged by size:
2424 1343 210
SbfI CC_TGCA'GG
Cuts at: 1024 1024
Size: 3977
ScaI AGT'ACT
Cuts at: 3494 3494
Size: 3977
SfcI C'TryA_G
Cuts at: 33 158 181 544 1020 1705 2698 2889 33
Size: 125 23 363 476 685 993 191 1121
Fragments arranged by size:
1121 993 685 476 363 191 125 23
SfoI GGC'GCC
Cuts at: 354 477 1409 1657 354
Size: 123 932 248 2674
Fragments arranged by size:
2674 932 248 123
SgfI GCG_AT'CGC
Cuts at: 104 104
Size: 3977
SmaI CCC'GGG
Cuts at: 999 999
Size: 3977
SmlI C'TyrA_G
Cuts at: 388 1132 2047 2539 2801 388
Size: 744 915 492 262 1564
Fragments arranged by size:
1564 915 744 492 262
SpeI A'CTAG_T
Cuts at: 67 67
Size: 3977
SphI G_CATG'C
Cuts at: 1030 1530 1030
Size: 500 3477
StyI C'CwwG_G
Cuts at: 107 303 1111 1465 107
Size: 196 808 354 2619
Fragments arranged by size:
2619 808 354 196
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 1560 1693 2152 2361 1560
Size: 133 459 209 3176
Fragments arranged by size:
3176 459 209 133
TatI w'GTAC_w
Cuts at: 3492 3492
Size: 3977
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 805 837 3494 3526 805
Size: 32 2657 32 1256
Fragments arranged by size:
2657 1256 32 32
Tth111I GACn'n_nGTC
Cuts at: 1774 1774
Size: 3977
XbaI T'CTAG_A
Cuts at: 1008 1008
Size: 3977
XcmI CCAnnnn_n'nnnnTGG
Cuts at: 919 1472 919
Size: 553 3424
XhoI C'TCGA_G
Cuts at: 388 388
Size: 3977
XmaI C'CCGG_G
Cuts at: 997 997
Size: 3977
Enzymes that do cut:
AccI Acc65I AclI AcuI AflIII AgeI AlwNI ApaLI
ApoI AseI AsiSI AvaI BamHI BanI BanII BbeI
BbvCI BciVI BclI BglI BglII BlpI Bme1580I BmrI
BplI BpmI Bpu10I BpuEI BsaI BsaAI BsaBI BsaHI
BsaWI BseRI BseYI BsgI BsiEI BsiHKAI BsmI Bsp1286I
BspEI BspHI BspMI BsrBI BsrDI BsrFI BssSI BstAPI
BstBI BstEII BstYI BtgI BtgZI BtsI DraI DrdI
EaeI EagI EarI EciI EcoICRI Eco57MI EcoNI EcoO109I
EcoRI FalI FspI HaeII Hin4I HincII KasI KpnI
MluI MmeI MscI MslI MspA1I NaeI NarI NcoI
NgoMIV NspI PciI PflMI PmeI PpiI PspXI PstI
PvuI PvuII RsrII SacI SalI SapI SbfI ScaI
SfcI SfoI SgfI SmaI SmlI SpeI SphI StyI
TaqII TatI TstI Tth111I XbaI XcmI XhoI XmaI
Enzymes that do not cut:
AarI AatII AfeI AflII AhdI AleI AlfI AloI
ApaI AscI AvrII BaeI BbsI BcgI BfrBI BmgBI
BmtI BsaXI BsiWI BsmBI BsrGI BssHII BstXI BstZ17I
Bsu36I ClaI CspCI DraIII EcoRV FseI FspAI HindIII
HpaI MfeI NdeI NheI NotI NruI NsiI PacI
PasI PfoI PmlI PpuMI PshAI PsiI PspOMI PsrI
SacII SanDI SexAI SfiI SgrAI SnaBI SrfI SspI
StuI SwaI XmnI ZraI