Restriction map for
pF1KB7583
(Circular) (Six-base) MAPSORT of: pF1KB7583.seq Check: 710 from: 1 to: 3776
pF1KB7583 3776 bp
With 182 enzymes: SgfI *
June 20, 2008 10:27 ..
AccI GT'mk_AC
Cuts at: 378 814 378
Size: 436 3340
Acc65I G'GTAC_C
Cuts at: 792 792
Size: 3776
AclI AA'CG_TT
Cuts at: 3616 3616
Size: 3776
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 457 514 1601 2033 2780 3048 457
Size: 57 1087 432 747 268 1185
Fragments arranged by size:
1185 1087 747 432 268 57
AfeI AGC'GCT
Cuts at: 568 568
Size: 3776
AflIII A'CryG_T
Cuts at: 1128 2232 3209 1128
Size: 1104 977 1695
Fragments arranged by size:
1695 1104 977
AgeI A'CCGG_T
Cuts at: 2164 2164
Size: 3776
AlwNI CAG_nnn'CTG
Cuts at: 141 1081 2648 141
Size: 940 1567 1269
Fragments arranged by size:
1567 1269 940
ApaLI G'TGCA_C
Cuts at: 2546 2546
Size: 3776
ApoI r'AATT_y
Cuts at: 780 3129 3385 780
Size: 2349 256 1171
Fragments arranged by size:
2349 1171 256
AseI AT'TA_AT
Cuts at: 20 20
Size: 3776
AsiSI GCG_AT'CGC
Cuts at: 104 104
Size: 3776
AvaI C'yCGr_G
Cuts at: 796 796
Size: 3776
BamHI G'GATC_C
Cuts at: 527 801 527
Size: 274 3502
BanI G'GyrC_C
Cuts at: 354 792 1206 1454 1489 354
Size: 438 414 248 35 2641
Fragments arranged by size:
2641 438 414 248 35
BanII G_rGCy'C
Cuts at: 318 790 1820 318
Size: 472 1030 2274
Fragments arranged by size:
2274 1030 472
BbeI G_GCGC'C
Cuts at: 1210 1458 1210
Size: 248 3528
BciVI GTATCCnnnnn_n'
Cuts at: 1674 2435 3776 1674
Size: 761 1341 1674
Fragments arranged by size:
1674 1341 761
BclI T'GATC_A
Cuts at: 1296 1296
Size: 3776
BglI GCCn_nnn'nGGC
Cuts at: 112 112
Size: 3776
BglII A'GATC_T
Cuts at: 1291 3328 1291
Size: 2037 1739
BlpI GC'TnA_GC
Cuts at: 888 888
Size: 3776
Bme1580I G_kGCm'C
Cuts at: 1401 1494 2550 1401
Size: 93 1056 2627
Fragments arranged by size:
2627 1056 93
BmgBI CAC'GTC
Cuts at: 603 603
Size: 3776
BmrI ACTGGGnnnn_n'
Cuts at: 370 988 1069 1404 3548 370
Size: 618 81 335 2144 598
Fragments arranged by size:
2144 618 598 335 81
BplI GAGnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 2143 2143
Size: 3776
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 166 184 355 526 660 166
Size: 18 171 171 134 3282
Fragments arranged by size:
3282 171 171 134 18
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 952 1831 2323 2621 952
Size: 879 492 298 2107
Fragments arranged by size:
2107 879 492 298
BsaI GGTCTCn'nnnn_
Cuts at: 772 3480 772
Size: 2708 1068
BsaAI yAC'GTr
Cuts at: 1129 1129
Size: 3776
BsaBI GATnn'nnATC
Cuts at: 6 130 1290 3327 6
Size: 124 1160 2037 455
Fragments arranged by size:
2037 1160 455 124
BsaHI Gr'CG_yC
Cuts at: 1207 1455 2157 3653 1207
Size: 248 702 1496 1330
Fragments arranged by size:
1496 1330 702 248
BsaWI w'CCGG_w
Cuts at: 530 965 1486 2164 2438 2585 2996 530
Size: 435 521 678 274 147 411 1310
Fragments arranged by size:
1310 678 521 435 411 274 147
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 549 549
Size: 3776
BseYI C'CCAG_C
Cuts at: 513 1201 1959 2536 3032 513
Size: 688 758 577 496 1257
Fragments arranged by size:
1257 758 688 577 496
BsiEI CG_ry'CG
Cuts at: 104 155 1318 1364 2572 104
Size: 51 1163 46 1208 1308
Fragments arranged by size:
1308 1208 1163 51 46
BsiHKAI G_wGCw'C
Cuts at: 613 790 1568 1758 2550 613
Size: 177 778 190 792 1839
Fragments arranged by size:
1839 792 778 190 177
BsmBI CGTCTCn'nnnn_
Cuts at: 561 561
Size: 3776
Bsp1286I G_dGCh'C
Cuts at: 318 613 790 1401 1494 1568 1758 1820 2550
Size: 295 177 611 93 74 190 62 730
Cuts at: 2550 318
Size: 1544
Fragments arranged by size:
1544 730 611 295 190 177 93 74
62
BspEI T'CCGG_A
Cuts at: 530 2996 530
Size: 2466 1310
BspHI T'CATG_A
Cuts at: 2952 2952
Size: 3776
BspMI ACCTGCnnnn'nnnn_
Cuts at: 826 1040 1342 1723 826
Size: 214 302 381 2879
Fragments arranged by size:
2879 381 302 214
BsrBI CCG'CTC
Cuts at: 50 168 672 2068 2122 3606 3771 50
Size: 118 504 1396 54 1484 165 55
Fragments arranged by size:
1484 1396 504 165 118 55 54
BsrDI GCAATG_nn'
Cuts at: 1688 1688
Size: 3776
BsrFI r'CCGG_y
Cuts at: 1774 1955 2164 1774
Size: 181 209 3386
Fragments arranged by size:
3386 209 181
BssSI C'ACGA_G
Cuts at: 2047 2405 2047
Size: 358 3418
BstAPI GCAn_nnn'nTGC
Cuts at: 1153 1153
Size: 3776
BstBI TT'CG_AA
Cuts at: 2137 2137
Size: 3776
BstYI r'GATC_y
Cuts at: 1 527 801 1268 1291 1626 1872 2884 2970
Size: 526 274 467 23 335 246 1012 86
Cuts at: 2970 2982 3328 1
Size: 12 346 449
Fragments arranged by size:
1012 526 467 449 346 335 274 246
86 23 12
BtgI C'CryG_G
Cuts at: 107 107
Size: 3776
BtgZI GCGATGnnnnnnnnnn'nnnn_
Cuts at: 1726 1907 2070 1726
Size: 181 163 3432
Fragments arranged by size:
3432 181 163
BtsI GCAGTG_nn'
Cuts at: 657 702 1019 657
Size: 45 317 3414
Fragments arranged by size:
3414 317 45
DraI TTT'AAA
Cuts at: 775 775
Size: 3776
DrdI GACnn_nn'nnGTC
Cuts at: 1482 2340 1482
Size: 858 2918
EaeI y'GGCC_r
Cuts at: 152 1361 1535 1926 1953 3298 152
Size: 1209 174 391 27 1345 630
Fragments arranged by size:
1345 1209 630 391 174 27
EagI C'GGCC_G
Cuts at: 152 1361 152
Size: 1209 2567
EarI CTCTTCn'nnn_
Cuts at: 490 1799 2009 490
Size: 1309 210 2257
Fragments arranged by size:
2257 1309 210
EciI GGCGGAnnnnnnnnn_nn'
Cuts at: 1984 2294 2440 3586 1984
Size: 310 146 1146 2174
Fragments arranged by size:
2174 1146 310 146
EcoICRI GAG'CTC
Cuts at: 788 788
Size: 3776
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 166 184 355 457 514 526 660 1601 2033
Size: 18 171 102 57 12 134 941 432
Cuts at: 2033 2780 3048 166
Size: 747 268 894
Fragments arranged by size:
941 894 747 432 268 171 134 102
57 18 12
EcoO109I rG'GnC_Cy
Cuts at: 364 915 364
Size: 551 3225
EcoRI G'AATT_C
Cuts at: 780 780
Size: 3776
FalI AAGnnnnnCTTnnnnnnnn_nnnnn'
Cuts at: 1795 1795
Size: 3776
FspI TGC'GCA
Cuts at: 1557 1557
Size: 3776
HaeII r_GCGC'y
Cuts at: 570 1210 1458 2480 3459 570
Size: 640 248 1022 979 887
Fragments arranged by size:
1022 979 887 640 248
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 1618 1648 1650 1680 1618
Size: 30 2 30 3714
Fragments arranged by size:
3714 30 30 2
HincII GTy'rAC
Cuts at: 379 600 815 379
Size: 221 215 3340
Fragments arranged by size:
3340 221 215
KasI G'GCGC_C
Cuts at: 1206 1454 1206
Size: 248 3528
KpnI G_GTAC'C
Cuts at: 796 796
Size: 3776
MluI A'CGCG_T
Cuts at: 3209 3209
Size: 3776
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 596 602 2447 2631 596
Size: 6 1845 184 1741
Fragments arranged by size:
1845 1741 184 6
MscI TGG'CCA
Cuts at: 1537 1537
Size: 3776
MslI CAynn'nnrTG
Cuts at: 136 194 1765 1892 136
Size: 58 1571 127 2020
Fragments arranged by size:
2020 1571 127 58
MspA1I CmG'CkG
Cuts at: 504 887 1201 1561 2574 2819 504
Size: 383 314 360 1013 245 1461
Fragments arranged by size:
1461 1013 383 360 314 245
NaeI GCC'GGC
Cuts at: 1957 1957
Size: 3776
NarI GG'CG_CC
Cuts at: 1207 1455 1207
Size: 248 3528
NcoI C'CATG_G
Cuts at: 107 107
Size: 3776
NgoMIV G'CCGG_C
Cuts at: 1955 1955
Size: 3776
NspI r_CATG'y
Cuts at: 684 829 1329 2236 684
Size: 145 500 907 2224
Fragments arranged by size:
2224 907 500 145
PciI A'CATG_T
Cuts at: 2232 2232
Size: 3776
PmeI GTTT'AAAC
Cuts at: 775 775
Size: 3776
PpiI GAACnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 2915 2947 2915
Size: 32 3744
PpuMI rG'GwC_Cy
Cuts at: 364 364
Size: 3776
PstI C_TGCA'G
Cuts at: 743 823 1508 743
Size: 80 685 3011
Fragments arranged by size:
3011 685 80
PvuI CG_AT'CG
Cuts at: 104 104
Size: 3776
PvuII CAG'CTG
Cuts at: 504 1201 1561 504
Size: 697 360 2719
Fragments arranged by size:
2719 697 360
RsrII CG'GwC_CG
Cuts at: 1971 1971
Size: 3776
SacI G_AGCT'C
Cuts at: 790 790
Size: 3776
SalI G'TCGA_C
Cuts at: 377 813 377
Size: 436 3340
SanDI GG'GwC_CC
Cuts at: 364 364
Size: 3776
SapI GCTCTTCn'nnn_
Cuts at: 1799 2009 1799
Size: 210 3566
SbfI CC_TGCA'GG
Cuts at: 823 823
Size: 3776
ScaI AGT'ACT
Cuts at: 3293 3293
Size: 3776
SfcI C'TryA_G
Cuts at: 33 739 819 1504 2497 2688 33
Size: 706 80 685 993 191 1121
Fragments arranged by size:
1121 993 706 685 191 80
SfoI GGC'GCC
Cuts at: 1208 1456 1208
Size: 248 3528
SgfI GCG_AT'CGC
Cuts at: 104 104
Size: 3776
SmaI CCC'GGG
Cuts at: 798 798
Size: 3776
SmlI C'TyrA_G
Cuts at: 931 1846 2338 2600 931
Size: 915 492 262 2107
Fragments arranged by size:
2107 915 492 262
SpeI A'CTAG_T
Cuts at: 67 67
Size: 3776
SphI G_CATG'C
Cuts at: 684 829 1329 684
Size: 145 500 3131
Fragments arranged by size:
3131 500 145
StyI C'CwwG_G
Cuts at: 107 910 1264 107
Size: 803 354 2619
Fragments arranged by size:
2619 803 354
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 1359 1492 1951 2160 1359
Size: 133 459 209 2975
Fragments arranged by size:
2975 459 209 133
TatI w'GTAC_w
Cuts at: 3291 3291
Size: 3776
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 3293 3325 3293
Size: 32 3744
Tth111I GACn'n_nGTC
Cuts at: 1573 1573
Size: 3776
XbaI T'CTAG_A
Cuts at: 807 807
Size: 3776
XcmI CCAnnnn_n'nnnnTGG
Cuts at: 1271 1271
Size: 3776
XmaI C'CCGG_G
Cuts at: 796 796
Size: 3776
Enzymes that do cut:
AccI Acc65I AclI AcuI AfeI AflIII AgeI AlwNI
ApaLI ApoI AseI AsiSI AvaI BamHI BanI BanII
BbeI BciVI BclI BglI BglII BlpI Bme1580I BmgBI
BmrI BplI BpmI BpuEI BsaI BsaAI BsaBI BsaHI
BsaWI BseRI BseYI BsiEI BsiHKAI BsmBI Bsp1286I BspEI
BspHI BspMI BsrBI BsrDI BsrFI BssSI BstAPI BstBI
BstYI BtgI BtgZI BtsI DraI DrdI EaeI EagI
EarI EciI EcoICRI Eco57MI EcoO109I EcoRI FalI FspI
HaeII Hin4I HincII KasI KpnI MluI MmeI MscI
MslI MspA1I NaeI NarI NcoI NgoMIV NspI PciI
PmeI PpiI PpuMI PstI PvuI PvuII RsrII SacI
SalI SanDI SapI SbfI ScaI SfcI SfoI SgfI
SmaI SmlI SpeI SphI StyI TaqII TatI TstI
Tth111I XbaI XcmI XmaI
Enzymes that do not cut:
AarI AatII AflII AhdI AleI AlfI AloI ApaI
AscI AvrII BaeI BbsI BbvCI BcgI BfrBI BmtI
Bpu10I BsaXI BsgI BsiWI BsmI BsrGI BssHII BstEII
BstXI BstZ17I Bsu36I ClaI CspCI DraIII EcoNI EcoRV
FseI FspAI HindIII HpaI MfeI NdeI NheI NotI
NruI NsiI PacI PasI PflMI PfoI PmlI PshAI
PsiI PspOMI PspXI PsrI SacII SexAI SfiI SgrAI
SnaBI SrfI SspI StuI SwaI XhoI XmnI ZraI