Restriction map for
pF1KB7582
(Circular) (Six-base) MAPSORT of: pF1KB7582.seq Check: 5506 from: 1 to: 3974
pF1KB7582 3974 bp
With 182 enzymes: SgfI *
March 1, 2013 10:20 ..
AccI GT'mk_AC
Cuts at: 1012 1012
Size: 3974
Acc65I G'GTAC_C
Cuts at: 990 990
Size: 3974
AclI AA'CG_TT
Cuts at: 3814 3814
Size: 3974
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 254 385 526 1799 2231 2978 3246 254
Size: 131 141 1273 432 747 268 982
Fragments arranged by size:
1273 982 747 432 268 141 131
AflIII A'CryG_T
Cuts at: 1326 2430 3407 1326
Size: 1104 977 1893
Fragments arranged by size:
1893 1104 977
AgeI A'CCGG_T
Cuts at: 2362 2362
Size: 3974
AlwNI CAG_nnn'CTG
Cuts at: 814 1279 2846 814
Size: 465 1567 1942
Fragments arranged by size:
1942 1567 465
ApaI G_GGCC'C
Cuts at: 342 342
Size: 3974
ApaLI G'TGCA_C
Cuts at: 2744 2744
Size: 3974
ApoI r'AATT_y
Cuts at: 655 978 3327 3583 655
Size: 323 2349 256 1046
Fragments arranged by size:
2349 1046 323 256
AseI AT'TA_AT
Cuts at: 20 20
Size: 3974
AsiSI GCG_AT'CGC
Cuts at: 104 104
Size: 3974
AvaI C'yCGr_G
Cuts at: 994 994
Size: 3974
BamHI G'GATC_C
Cuts at: 516 999 516
Size: 483 3491
BanI G'GyrC_C
Cuts at: 990 1404 1652 1687 990
Size: 414 248 35 3277
Fragments arranged by size:
3277 414 248 35
BanII G_rGCy'C
Cuts at: 342 935 988 2018 342
Size: 593 53 1030 2298
Fragments arranged by size:
2298 1030 593 53
BbeI G_GCGC'C
Cuts at: 1408 1656 1408
Size: 248 3726
BciVI GTATCCnnnnn_n'
Cuts at: 1872 2633 3974 1872
Size: 761 1341 1872
Fragments arranged by size:
1872 1341 761
BclI T'GATC_A
Cuts at: 1494 1494
Size: 3974
BglII A'GATC_T
Cuts at: 1489 3526 1489
Size: 2037 1937
BlpI GC'TnA_GC
Cuts at: 1086 1086
Size: 3974
Bme1580I G_kGCm'C
Cuts at: 342 1599 1692 2748 342
Size: 1257 93 1056 1568
Fragments arranged by size:
1568 1257 1056 93
BmrI ACTGGGnnnn_n'
Cuts at: 866 1186 1267 1602 3746 866
Size: 320 81 335 2144 1094
Fragments arranged by size:
2144 1094 335 320 81
BplI GAGnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 2341 2341
Size: 3974
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 896 896
Size: 3974
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 1150 2029 2521 2819 1150
Size: 879 492 298 2305
Fragments arranged by size:
2305 879 492 298
BsaI GGTCTCn'nnnn_
Cuts at: 3678 3678
Size: 3974
BsaAI yAC'GTr
Cuts at: 1327 1327
Size: 3974
BsaBI GATnn'nnATC
Cuts at: 6 1488 3525 6
Size: 1482 2037 455
Fragments arranged by size:
2037 1482 455
BsaHI Gr'CG_yC
Cuts at: 1405 1653 2355 3851 1405
Size: 248 702 1496 1528
Fragments arranged by size:
1528 1496 702 248
BsaWI w'CCGG_w
Cuts at: 1163 1684 2362 2636 2783 3194 1163
Size: 521 678 274 147 411 1943
Fragments arranged by size:
1943 678 521 411 274 147
BsaXI ACnnnnnCTCCnnnnnnn_nnn'
Cuts at: 296 326 296
Size: 30 3944
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 301 304 628 816 301
Size: 3 324 188 3459
Fragments arranged by size:
3459 324 188 3
BseYI C'CCAG_C
Cuts at: 341 365 1399 2157 2734 3230 341
Size: 24 1034 758 577 496 1085
Fragments arranged by size:
1085 1034 758 577 496 24
BsiEI CG_ry'CG
Cuts at: 104 1516 1562 2770 104
Size: 1412 46 1208 1308
Fragments arranged by size:
1412 1308 1208 46
BsiHKAI G_wGCw'C
Cuts at: 935 988 1766 1956 2748 935
Size: 53 778 190 792 2161
Fragments arranged by size:
2161 792 778 190 53
Bsp1286I G_dGCh'C
Cuts at: 342 935 988 1599 1692 1766 1956 2018 2748
Size: 593 53 611 93 74 190 62 730
Cuts at: 2748 342
Size: 1568
Fragments arranged by size:
1568 730 611 593 190 93 74 62
53
BspEI T'CCGG_A
Cuts at: 3194 3194
Size: 3974
BspHI T'CATG_A
Cuts at: 3150 3150
Size: 3974
BspMI ACCTGCnnnn'nnnn_
Cuts at: 1024 1238 1540 1921 1024
Size: 214 302 381 3077
Fragments arranged by size:
3077 381 302 214
BsrBI CCG'CTC
Cuts at: 50 2266 2320 3804 3969 50
Size: 2216 54 1484 165 55
Fragments arranged by size:
2216 1484 165 55 54
BsrDI GCAATG_nn'
Cuts at: 1886 1886
Size: 3974
BsrFI r'CCGG_y
Cuts at: 1972 2153 2362 1972
Size: 181 209 3584
Fragments arranged by size:
3584 209 181
BssSI C'ACGA_G
Cuts at: 2245 2603 2245
Size: 358 3616
BstAPI GCAn_nnn'nTGC
Cuts at: 430 814 1351 430
Size: 384 537 3053
Fragments arranged by size:
3053 537 384
BstBI TT'CG_AA
Cuts at: 2335 2335
Size: 3974
BstYI r'GATC_y
Cuts at: 1 503 516 999 1466 1489 1824 2070 3082
Size: 502 13 483 467 23 335 246 1012
Cuts at: 3082 3168 3180 3526 1
Size: 86 12 346 449
Fragments arranged by size:
1012 502 483 467 449 346 335 246
86 23 13 12
BtgI C'CryG_G
Cuts at: 107 107
Size: 3974
BtgZI GCGATGnnnnnnnnnn'nnnn_
Cuts at: 1924 2105 2268 1924
Size: 181 163 3630
Fragments arranged by size:
3630 181 163
BtsI GCAGTG_nn'
Cuts at: 1217 1217
Size: 3974
DraI TTT'AAA
Cuts at: 973 973
Size: 3974
DrdI GACnn_nn'nnGTC
Cuts at: 1680 2538 1680
Size: 858 3116
EaeI y'GGCC_r
Cuts at: 1559 1733 2124 2151 3496 1559
Size: 174 391 27 1345 2037
Fragments arranged by size:
2037 1345 391 174 27
EagI C'GGCC_G
Cuts at: 1559 1559
Size: 3974
EarI CTCTTCn'nnn_
Cuts at: 185 637 1997 2207 185
Size: 452 1360 210 1952
Fragments arranged by size:
1952 1360 452 210
EciI GGCGGAnnnnnnnnn_nn'
Cuts at: 2182 2492 2638 3784 2182
Size: 310 146 1146 2372
Fragments arranged by size:
2372 1146 310 146
EcoICRI GAG'CTC
Cuts at: 933 986 933
Size: 53 3921
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 254 385 526 896 1799 2231 2978 3246 254
Size: 131 141 370 903 432 747 268 982
Fragments arranged by size:
982 903 747 432 370 268 141 131
EcoO109I rG'GnC_Cy
Cuts at: 124 338 1113 124
Size: 214 775 2985
Fragments arranged by size:
2985 775 214
EcoRI G'AATT_C
Cuts at: 655 978 655
Size: 323 3651
FalI AAGnnnnnCTTnnnnnnnn_nnnnn'
Cuts at: 1993 1993
Size: 3974
FspI TGC'GCA
Cuts at: 1755 1755
Size: 3974
HaeII r_GCGC'y
Cuts at: 1408 1656 2678 3657 1408
Size: 248 1022 979 1725
Fragments arranged by size:
1725 1022 979 248
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 892 924 1816 1846 1848 1878 892
Size: 32 892 30 2 30 2988
Fragments arranged by size:
2988 892 32 30 30 2
HincII GTy'rAC
Cuts at: 1013 1013
Size: 3974
KasI G'GCGC_C
Cuts at: 1404 1652 1404
Size: 248 3726
KpnI G_GTAC'C
Cuts at: 994 994
Size: 3974
MluI A'CGCG_T
Cuts at: 3407 3407
Size: 3974
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 2645 2829 2645
Size: 184 3790
MscI TGG'CCA
Cuts at: 1735 1735
Size: 3974
MslI CAynn'nnrTG
Cuts at: 1963 2090 1963
Size: 127 3847
MspA1I CmG'CkG
Cuts at: 391 1085 1399 1759 2772 3017 391
Size: 694 314 360 1013 245 1348
Fragments arranged by size:
1348 1013 694 360 314 245
NaeI GCC'GGC
Cuts at: 2155 2155
Size: 3974
NarI GG'CG_CC
Cuts at: 1405 1653 1405
Size: 248 3726
NcoI C'CATG_G
Cuts at: 107 107
Size: 3974
NdeI CA'TA_TG
Cuts at: 299 299
Size: 3974
NgoMIV G'CCGG_C
Cuts at: 2153 2153
Size: 3974
NspI r_CATG'y
Cuts at: 652 808 1027 1527 2434 652
Size: 156 219 500 907 2192
Fragments arranged by size:
2192 907 500 219 156
PciI A'CATG_T
Cuts at: 2430 2430
Size: 3974
PflMI CCAn_nnn'nTGG
Cuts at: 574 574
Size: 3974
PmeI GTTT'AAAC
Cuts at: 973 973
Size: 3974
PpiI GAACnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 3113 3145 3113
Size: 32 3942
PpuMI rG'GwC_Cy
Cuts at: 124 124
Size: 3974
PspOMI G'GGCC_C
Cuts at: 338 338
Size: 3974
PstI C_TGCA'G
Cuts at: 180 222 426 1021 1706 180
Size: 42 204 595 685 2448
Fragments arranged by size:
2448 685 595 204 42
PvuI CG_AT'CG
Cuts at: 104 104
Size: 3974
PvuII CAG'CTG
Cuts at: 391 1399 1759 391
Size: 1008 360 2606
Fragments arranged by size:
2606 1008 360
RsrII CG'GwC_CG
Cuts at: 2169 2169
Size: 3974
SacI G_AGCT'C
Cuts at: 935 988 935
Size: 53 3921
SalI G'TCGA_C
Cuts at: 1011 1011
Size: 3974
SapI GCTCTTCn'nnn_
Cuts at: 1997 2207 1997
Size: 210 3764
SbfI CC_TGCA'GG
Cuts at: 1021 1021
Size: 3974
ScaI AGT'ACT
Cuts at: 3491 3491
Size: 3974
SfcI C'TryA_G
Cuts at: 33 176 218 422 818 1017 1702 2695 2886
Size: 143 42 204 396 199 685 993 191
Cuts at: 2886 33
Size: 1121
Fragments arranged by size:
1121 993 685 396 204 199 191 143
42
SfoI GGC'GCC
Cuts at: 1406 1654 1406
Size: 248 3726
SgfI GCG_AT'CGC
Cuts at: 104 104
Size: 3974
SmaI CCC'GGG
Cuts at: 996 996
Size: 3974
SmlI C'TyrA_G
Cuts at: 1129 2044 2536 2798 1129
Size: 915 492 262 2305
Fragments arranged by size:
2305 915 492 262
SpeI A'CTAG_T
Cuts at: 67 67
Size: 3974
SphI G_CATG'C
Cuts at: 1027 1527 1027
Size: 500 3474
StyI C'CwwG_G
Cuts at: 107 866 896 1108 1462 107
Size: 759 30 212 354 2619
Fragments arranged by size:
2619 759 354 212 30
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 1557 1690 2149 2358 1557
Size: 133 459 209 3173
Fragments arranged by size:
3173 459 209 133
TatI w'GTAC_w
Cuts at: 461 3489 461
Size: 3028 946
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 3491 3523 3491
Size: 32 3942
Tth111I GACn'n_nGTC
Cuts at: 252 1771 252
Size: 1519 2455
XbaI T'CTAG_A
Cuts at: 1005 1005
Size: 3974
XcmI CCAnnnn_n'nnnnTGG
Cuts at: 114 1469 114
Size: 1355 2619
XmaI C'CCGG_G
Cuts at: 994 994
Size: 3974
Enzymes that do cut:
AccI Acc65I AclI AcuI AflIII AgeI AlwNI ApaI
ApaLI ApoI AseI AsiSI AvaI BamHI BanI BanII
BbeI BciVI BclI BglII BlpI Bme1580I BmrI BplI
BpmI BpuEI BsaI BsaAI BsaBI BsaHI BsaWI BsaXI
BseRI BseYI BsiEI BsiHKAI Bsp1286I BspEI BspHI BspMI
BsrBI BsrDI BsrFI BssSI BstAPI BstBI BstYI BtgI
BtgZI BtsI DraI DrdI EaeI EagI EarI EciI
EcoICRI Eco57MI EcoO109I EcoRI FalI FspI HaeII Hin4I
HincII KasI KpnI MluI MmeI MscI MslI MspA1I
NaeI NarI NcoI NdeI NgoMIV NspI PciI PflMI
PmeI PpiI PpuMI PspOMI PstI PvuI PvuII RsrII
SacI SalI SapI SbfI ScaI SfcI SfoI SgfI
SmaI SmlI SpeI SphI StyI TaqII TatI TstI
Tth111I XbaI XcmI XmaI
Enzymes that do not cut:
AarI AatII AfeI AflII AhdI AleI AlfI AloI
AscI AvrII BaeI BbsI BbvCI BcgI BfrBI BglI
BmgBI BmtI Bpu10I BsgI BsiWI BsmI BsmBI BsrGI
BssHII BstEII BstXI BstZ17I Bsu36I ClaI CspCI DraIII
EcoNI EcoRV FseI FspAI HindIII HpaI MfeI NheI
NotI NruI NsiI PacI PasI PfoI PmlI PshAI
PsiI PspXI PsrI SacII SanDI SexAI SfiI SgrAI
SnaBI SrfI SspI StuI SwaI XhoI XmnI ZraI