Restriction map for
pF1KB7574
(Circular) (Six-base) MAPSORT of: pF1KB7574.seq Check: 8775 from: 1 to: 3956
pF1KB7574 3956 bp
With 182 enzymes: SgfI *
June 20, 2008 10:27 ..
AccI GT'mk_AC
Cuts at: 994 994
Size: 3956
Acc65I G'GTAC_C
Cuts at: 972 972
Size: 3956
AclI AA'CG_TT
Cuts at: 950 3796 950
Size: 2846 1110
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 872 1781 2213 2960 3228 872
Size: 909 432 747 268 1600
Fragments arranged by size:
1600 909 747 432 268
AflIII A'CryG_T
Cuts at: 1308 2412 3389 1308
Size: 1104 977 1875
Fragments arranged by size:
1875 1104 977
AgeI A'CCGG_T
Cuts at: 2344 2344
Size: 3956
AlwNI CAG_nnn'CTG
Cuts at: 609 1261 2828 609
Size: 652 1567 1737
Fragments arranged by size:
1737 1567 652
ApaLI G'TGCA_C
Cuts at: 772 2726 772
Size: 1954 2002
ApoI r'AATT_y
Cuts at: 328 960 3309 3565 328
Size: 632 2349 256 719
Fragments arranged by size:
2349 719 632 256
AseI AT'TA_AT
Cuts at: 20 233 20
Size: 213 3743
AsiSI GCG_AT'CGC
Cuts at: 104 104
Size: 3956
AvaI C'yCGr_G
Cuts at: 355 976 355
Size: 621 3335
BamHI G'GATC_C
Cuts at: 981 981
Size: 3956
BanI G'GyrC_C
Cuts at: 182 972 1386 1634 1669 182
Size: 790 414 248 35 2469
Fragments arranged by size:
2469 790 414 248 35
BanII G_rGCy'C
Cuts at: 970 2000 970
Size: 1030 2926
BbeI G_GCGC'C
Cuts at: 1390 1638 1390
Size: 248 3708
BbsI GAAGACnn'nnnn_
Cuts at: 764 764
Size: 3956
BbvCI CC'TCA_GC
Cuts at: 917 917
Size: 3956
BciVI GTATCCnnnnn_n'
Cuts at: 1854 2615 3956 1854
Size: 761 1341 1854
Fragments arranged by size:
1854 1341 761
BclI T'GATC_A
Cuts at: 1476 1476
Size: 3956
BglII A'GATC_T
Cuts at: 346 1471 3508 346
Size: 1125 2037 794
Fragments arranged by size:
2037 1125 794
BlpI GC'TnA_GC
Cuts at: 602 1068 602
Size: 466 3490
Bme1580I G_kGCm'C
Cuts at: 776 1581 1674 2730 776
Size: 805 93 1056 2002
Fragments arranged by size:
2002 1056 805 93
BmrI ACTGGGnnnn_n'
Cuts at: 134 403 1168 1249 1584 3728 134
Size: 269 765 81 335 2144 362
Fragments arranged by size:
2144 765 362 335 269 81
BplI GAGnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 290 2323 290
Size: 2033 1923
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 704 704
Size: 3956
Bpu10I CC'TnA_GC
Cuts at: 917 917
Size: 3956
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 1132 2011 2503 2801 1132
Size: 879 492 298 2287
Fragments arranged by size:
2287 879 492 298
BsaI GGTCTCn'nnnn_
Cuts at: 182 282 3660 182
Size: 100 3378 478
Fragments arranged by size:
3378 478 100
BsaAI yAC'GTr
Cuts at: 1309 1309
Size: 3956
BsaBI GATnn'nnATC
Cuts at: 6 1470 3507 6
Size: 1464 2037 455
Fragments arranged by size:
2037 1464 455
BsaHI Gr'CG_yC
Cuts at: 1387 1635 2337 3833 1387
Size: 248 702 1496 1510
Fragments arranged by size:
1510 1496 702 248
BsaWI w'CCGG_w
Cuts at: 1145 1666 2344 2618 2765 3176 1145
Size: 521 678 274 147 411 1925
Fragments arranged by size:
1925 678 521 411 274 147
BsaXI ACnnnnnCTCCnnnnnnn_nnn'
Cuts at: 128 158 128
Size: 30 3926
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 136 178 136
Size: 42 3914
BseYI C'CCAG_C
Cuts at: 1381 2139 2716 3212 1381
Size: 758 577 496 2125
Fragments arranged by size:
2125 758 577 496
BsiEI CG_ry'CG
Cuts at: 104 124 1498 1544 2752 104
Size: 20 1374 46 1208 1308
Fragments arranged by size:
1374 1308 1208 46 20
BsiHKAI G_wGCw'C
Cuts at: 603 776 787 883 970 1748 1938 2730 603
Size: 173 11 96 87 778 190 792 1829
Fragments arranged by size:
1829 792 778 190 173 96 87 11
BsmI GAATG_Cn'
Cuts at: 677 677
Size: 3956
BsmBI CGTCTCn'nnnn_
Cuts at: 160 160
Size: 3956
Bsp1286I G_dGCh'C
Cuts at: 603 776 787 883 970 1581 1674 1748 1938
Size: 173 11 96 87 611 93 74 190
Cuts at: 1938 2000 2730 603
Size: 62 730 1829
Fragments arranged by size:
1829 730 611 190 173 96 93 87
74 62 11
BspEI T'CCGG_A
Cuts at: 3176 3176
Size: 3956
BspHI T'CATG_A
Cuts at: 3132 3132
Size: 3956
BspMI ACCTGCnnnn'nnnn_
Cuts at: 1006 1220 1522 1903 1006
Size: 214 302 381 3059
Fragments arranged by size:
3059 381 302 214
BsrBI CCG'CTC
Cuts at: 50 2248 2302 3786 3951 50
Size: 2198 54 1484 165 55
Fragments arranged by size:
2198 1484 165 55 54
BsrDI GCAATG_nn'
Cuts at: 569 704 1868 569
Size: 135 1164 2657
Fragments arranged by size:
2657 1164 135
BsrFI r'CCGG_y
Cuts at: 123 1954 2135 2344 123
Size: 1831 181 209 1735
Fragments arranged by size:
1831 1735 209 181
BsrGI T'GTAC_A
Cuts at: 249 249
Size: 3956
BssSI C'ACGA_G
Cuts at: 2227 2585 2227
Size: 358 3598
BstAPI GCAn_nnn'nTGC
Cuts at: 1333 1333
Size: 3956
BstBI TT'CG_AA
Cuts at: 2317 2317
Size: 3956
BstXI CCAn_nnnn'nTGG
Cuts at: 201 201
Size: 3956
BstYI r'GATC_y
Cuts at: 1 336 346 490 981 1448 1471 1806 2052
Size: 335 10 144 491 467 23 335 246
Cuts at: 2052 3064 3150 3162 3508 1
Size: 1012 86 12 346 449
Fragments arranged by size:
1012 491 467 449 346 335 335 246
144 86 23 12 10
Bsu36I CC'TnA_GG
Cuts at: 899 899
Size: 3956
BtgZI GCGATGnnnnnnnnnn'nnnn_
Cuts at: 1906 2087 2250 1906
Size: 181 163 3612
Fragments arranged by size:
3612 181 163
BtsI GCAGTG_nn'
Cuts at: 1199 1199
Size: 3956
ClaI AT'CG_AT
Cuts at: 800 800
Size: 3956
DraI TTT'AAA
Cuts at: 955 955
Size: 3956
DrdI GACnn_nn'nnGTC
Cuts at: 1662 2520 1662
Size: 858 3098
EaeI y'GGCC_r
Cuts at: 121 1541 1715 2106 2133 3478 121
Size: 1420 174 391 27 1345 599
Fragments arranged by size:
1420 1345 599 391 174 27
EagI C'GGCC_G
Cuts at: 121 1541 121
Size: 1420 2536
EarI CTCTTCn'nnn_
Cuts at: 280 873 1979 2189 280
Size: 593 1106 210 2047
Fragments arranged by size:
2047 1106 593 210
EciI GGCGGAnnnnnnnnn_nn'
Cuts at: 2164 2474 2620 3766 2164
Size: 310 146 1146 2354
Fragments arranged by size:
2354 1146 310 146
EcoICRI GAG'CTC
Cuts at: 968 968
Size: 3956
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 704 872 1781 2213 2960 3228 704
Size: 168 909 432 747 268 1432
Fragments arranged by size:
1432 909 747 432 268 168
EcoO109I rG'GnC_Cy
Cuts at: 207 863 895 1095 207
Size: 656 32 200 3068
Fragments arranged by size:
3068 656 200 32
EcoRI G'AATT_C
Cuts at: 960 960
Size: 3956
FalI AAGnnnnnCTTnnnnnnnn_nnnnn'
Cuts at: 952 1975 952
Size: 1023 2933
FspI TGC'GCA
Cuts at: 1737 1737
Size: 3956
HaeII r_GCGC'y
Cuts at: 1390 1638 2660 3639 1390
Size: 248 1022 979 1707
Fragments arranged by size:
1707 1022 979 248
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 179 211 870 902 1798 1828 1830 1860 179
Size: 32 659 32 896 30 2 30 2275
Fragments arranged by size:
2275 896 659 32 32 30 30 2
HincII GTy'rAC
Cuts at: 620 995 620
Size: 375 3581
HpaI GTT'AAC
Cuts at: 620 620
Size: 3956
KasI G'GCGC_C
Cuts at: 1386 1634 1386
Size: 248 3708
KpnI G_GTAC'C
Cuts at: 976 976
Size: 3956
MluI A'CGCG_T
Cuts at: 3389 3389
Size: 3956
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 2627 2811 2627
Size: 184 3772
MscI TGG'CCA
Cuts at: 1717 1717
Size: 3956
MslI CAynn'nnrTG
Cuts at: 199 1945 2072 199
Size: 1746 127 2083
Fragments arranged by size:
2083 1746 127
MspA1I CmG'CkG
Cuts at: 120 609 1067 1381 1741 2754 2999 120
Size: 489 458 314 360 1013 245 1077
Fragments arranged by size:
1077 1013 489 458 360 314 245
NaeI GCC'GGC
Cuts at: 125 2137 125
Size: 2012 1944
NarI GG'CG_CC
Cuts at: 1387 1635 1387
Size: 248 3708
NdeI CA'TA_TG
Cuts at: 651 651
Size: 3956
NgoMIV G'CCGG_C
Cuts at: 123 2135 123
Size: 2012 1944
NspI r_CATG'y
Cuts at: 1009 1509 2416 1009
Size: 500 907 2549
Fragments arranged by size:
2549 907 500
PacI TTA_AT'TAA
Cuts at: 590 590
Size: 3956
PciI A'CATG_T
Cuts at: 2412 2412
Size: 3956
PfoI T'CCnGG_A
Cuts at: 149 149
Size: 3956
PmeI GTTT'AAAC
Cuts at: 955 955
Size: 3956
PpiI GAACnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 3095 3127 3095
Size: 32 3924
PpuMI rG'GwC_Cy
Cuts at: 863 863
Size: 3956
PstI C_TGCA'G
Cuts at: 1003 1688 1003
Size: 685 3271
PvuI CG_AT'CG
Cuts at: 104 104
Size: 3956
PvuII CAG'CTG
Cuts at: 609 1381 1741 609
Size: 772 360 2824
Fragments arranged by size:
2824 772 360
RsrII CG'GwC_CG
Cuts at: 2151 2151
Size: 3956
SacI G_AGCT'C
Cuts at: 970 970
Size: 3956
SalI G'TCGA_C
Cuts at: 993 993
Size: 3956
SapI GCTCTTCn'nnn_
Cuts at: 1979 2189 1979
Size: 210 3746
SbfI CC_TGCA'GG
Cuts at: 1003 1003
Size: 3956
ScaI AGT'ACT
Cuts at: 3473 3473
Size: 3956
SfcI C'TryA_G
Cuts at: 33 535 999 1684 2677 2868 33
Size: 502 464 685 993 191 1121
Fragments arranged by size:
1121 993 685 502 464 191
SfoI GGC'GCC
Cuts at: 1388 1636 1388
Size: 248 3708
SgfI GCG_AT'CGC
Cuts at: 104 104
Size: 3956
SmaI CCC'GGG
Cuts at: 357 978 357
Size: 621 3335
SmlI C'TyrA_G
Cuts at: 1111 2026 2518 2780 1111
Size: 915 492 262 2287
Fragments arranged by size:
2287 915 492 262
SpeI A'CTAG_T
Cuts at: 67 67
Size: 3956
SphI G_CATG'C
Cuts at: 1009 1509 1009
Size: 500 3456
StuI AGG'CCT
Cuts at: 685 685
Size: 3956
StyI C'CwwG_G
Cuts at: 1090 1444 1090
Size: 354 3602
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 1539 1672 2131 2340 1539
Size: 133 459 209 3155
Fragments arranged by size:
3155 459 209 133
TatI w'GTAC_w
Cuts at: 249 3471 249
Size: 3222 734
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 3473 3505 3473
Size: 32 3924
Tth111I GACn'n_nGTC
Cuts at: 1753 1753
Size: 3956
XbaI T'CTAG_A
Cuts at: 987 987
Size: 3956
XcmI CCAnnnn_n'nnnnTGG
Cuts at: 147 1451 147
Size: 1304 2652
XmaI C'CCGG_G
Cuts at: 355 976 355
Size: 621 3335
Enzymes that do cut:
AccI Acc65I AclI AcuI AflIII AgeI AlwNI ApaLI
ApoI AseI AsiSI AvaI BamHI BanI BanII BbeI
BbsI BbvCI BciVI BclI BglII BlpI Bme1580I BmrI
BplI BpmI Bpu10I BpuEI BsaI BsaAI BsaBI BsaHI
BsaWI BsaXI BseRI BseYI BsiEI BsiHKAI BsmI BsmBI
Bsp1286I BspEI BspHI BspMI BsrBI BsrDI BsrFI BsrGI
BssSI BstAPI BstBI BstXI BstYI Bsu36I BtgZI BtsI
ClaI DraI DrdI EaeI EagI EarI EciI EcoICRI
Eco57MI EcoO109I EcoRI FalI FspI HaeII Hin4I HincII
HpaI KasI KpnI MluI MmeI MscI MslI MspA1I
NaeI NarI NdeI NgoMIV NspI PacI PciI PfoI
PmeI PpiI PpuMI PstI PvuI PvuII RsrII SacI
SalI SapI SbfI ScaI SfcI SfoI SgfI SmaI
SmlI SpeI SphI StuI StyI TaqII TatI TstI
Tth111I XbaI XcmI XmaI
Enzymes that do not cut:
AarI AatII AfeI AflII AhdI AleI AlfI AloI
ApaI AscI AvrII BaeI BcgI BfrBI BglI BmgBI
BmtI BsgI BsiWI BssHII BstEII BstZ17I BtgI CspCI
DraIII EcoNI EcoRV FseI FspAI HindIII MfeI NcoI
NheI NotI NruI NsiI PasI PflMI PmlI PshAI
PsiI PspOMI PspXI PsrI SacII SanDI SexAI SfiI
SgrAI SnaBI SrfI SspI SwaI XhoI XmnI ZraI