Restriction map for
pF1KB7572
(Circular) (Six-base) MAPSORT of: pF1KB7572.seq Check: 2388 from: 1 to: 3953
pF1KB7572 3953 bp
With 182 enzymes: SgfI *
June 20, 2008 10:27 ..
AarI CACCTGCnnnn'nnnn_
Cuts at: 219 219
Size: 3953
AccI GT'mk_AC
Cuts at: 446 991 446
Size: 545 3408
Acc65I G'GTAC_C
Cuts at: 969 969
Size: 3953
AclI AA'CG_TT
Cuts at: 602 3793 602
Size: 3191 762
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 1778 2210 2957 3225 1778
Size: 432 747 268 2506
Fragments arranged by size:
2506 747 432 268
AfeI AGC'GCT
Cuts at: 586 586
Size: 3953
AflIII A'CryG_T
Cuts at: 1305 2409 3386 1305
Size: 1104 977 1872
Fragments arranged by size:
1872 1104 977
AgeI A'CCGG_T
Cuts at: 2341 2341
Size: 3953
AlwNI CAG_nnn'CTG
Cuts at: 190 1258 2825 190
Size: 1068 1567 1318
Fragments arranged by size:
1567 1318 1068
ApaI G_GGCC'C
Cuts at: 142 224 142
Size: 82 3871
ApaLI G'TGCA_C
Cuts at: 787 2723 787
Size: 1936 2017
ApoI r'AATT_y
Cuts at: 957 3306 3562 957
Size: 2349 256 1348
Fragments arranged by size:
2349 1348 256
AseI AT'TA_AT
Cuts at: 20 20
Size: 3953
AsiSI GCG_AT'CGC
Cuts at: 104 104
Size: 3953
AvaI C'yCGr_G
Cuts at: 766 973 766
Size: 207 3746
BamHI G'GATC_C
Cuts at: 820 978 820
Size: 158 3795
BanI G'GyrC_C
Cuts at: 536 610 808 969 1383 1631 1666 536
Size: 74 198 161 414 248 35 2823
Fragments arranged by size:
2823 414 248 198 161 74 35
BanII G_rGCy'C
Cuts at: 142 224 937 967 1997 142
Size: 82 713 30 1030 2098
Fragments arranged by size:
2098 1030 713 82 30
BbeI G_GCGC'C
Cuts at: 614 812 1387 1635 614
Size: 198 575 248 2932
Fragments arranged by size:
2932 575 248 198
BbvCI CC'TCA_GC
Cuts at: 530 625 530
Size: 95 3858
BcgI CGAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 833 867 833
Size: 34 3919
BciVI GTATCCnnnnn_n'
Cuts at: 1851 2612 3953 1851
Size: 761 1341 1851
Fragments arranged by size:
1851 1341 761
BclI T'GATC_A
Cuts at: 293 1473 293
Size: 1180 2773
BglI GCCn_nnn'nGGC
Cuts at: 270 841 270
Size: 571 3382
BglII A'GATC_T
Cuts at: 1468 3505 1468
Size: 2037 1916
BlpI GC'TnA_GC
Cuts at: 664 1065 664
Size: 401 3552
Bme1580I G_kGCm'C
Cuts at: 115 142 177 224 352 539 791 1578 1671
Size: 27 35 47 128 187 252 787 93
Cuts at: 1671 2727 115
Size: 1056 1341
Fragments arranged by size:
1341 1056 787 252 187 128 93 47
35 27
BmrI ACTGGGnnnn_n'
Cuts at: 1165 1246 1581 3725 1165
Size: 81 335 2144 1393
Fragments arranged by size:
2144 1393 335 81
BplI GAGnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 2320 2320
Size: 3953
Bpu10I CC'TnA_GC
Cuts at: 203 530 625 203
Size: 327 95 3531
Fragments arranged by size:
3531 327 95
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 1129 2008 2500 2798 1129
Size: 879 492 298 2284
Fragments arranged by size:
2284 879 492 298
BsaI GGTCTCn'nnnn_
Cuts at: 495 3657 495
Size: 3162 791
BsaAI yAC'GTr
Cuts at: 1306 1306
Size: 3953
BsaBI GATnn'nnATC
Cuts at: 6 1467 3504 6
Size: 1461 2037 455
Fragments arranged by size:
2037 1461 455
BsaHI Gr'CG_yC
Cuts at: 611 809 1384 1632 2334 3830 611
Size: 198 575 248 702 1496 734
Fragments arranged by size:
1496 734 702 575 248 198
BsaWI w'CCGG_w
Cuts at: 656 823 1142 1663 2341 2615 2762 3173 656
Size: 167 319 521 678 274 147 411 1436
Fragments arranged by size:
1436 678 521 411 319 274 167 147
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 249 250 266 249
Size: 1 16 3936
Fragments arranged by size:
3936 16 1
BseYI C'CCAG_C
Cuts at: 178 183 345 1378 2136 2713 3209 178
Size: 5 162 1033 758 577 496 922
Fragments arranged by size:
1033 922 758 577 496 162 5
BsgI GTGCAGnnnnnnnnnnnnnn_nn'
Cuts at: 397 397
Size: 3953
BsiEI CG_ry'CG
Cuts at: 104 657 784 872 1495 1541 2749 104
Size: 553 127 88 623 46 1208 1308
Fragments arranged by size:
1308 1208 623 553 127 88 46
BsiHKAI G_wGCw'C
Cuts at: 683 791 967 1745 1935 2727 683
Size: 108 176 778 190 792 1909
Fragments arranged by size:
1909 792 778 190 176 108
Bsp1286I G_dGCh'C
Cuts at: 115 142 177 224 352 539 683 791 937
Size: 27 35 47 128 187 144 108 146
Cuts at: 937 967 1578 1671 1745 1935 1997 2727 115
Size: 30 611 93 74 190 62 730 1341
Fragments arranged by size:
1341 730 611 190 187 146 144 128
108 93 74 62 47 35 30 27
BspEI T'CCGG_A
Cuts at: 823 3173 823
Size: 2350 1603
BspHI T'CATG_A
Cuts at: 3129 3129
Size: 3953
BspMI ACCTGCnnnn'nnnn_
Cuts at: 219 1003 1217 1519 1900 219
Size: 784 214 302 381 2272
Fragments arranged by size:
2272 784 381 302 214
BsrBI CCG'CTC
Cuts at: 50 898 2245 2299 3783 3948 50
Size: 848 1347 54 1484 165 55
Fragments arranged by size:
1484 1347 848 165 55 54
BsrDI GCAATG_nn'
Cuts at: 1865 1865
Size: 3953
BsrFI r'CCGG_y
Cuts at: 488 783 1951 2132 2341 488
Size: 295 1168 181 209 2100
Fragments arranged by size:
2100 1168 295 209 181
BssHII G'CGCG_C
Cuts at: 847 847
Size: 3953
BssSI C'ACGA_G
Cuts at: 2224 2582 2224
Size: 358 3595
BstAPI GCAn_nnn'nTGC
Cuts at: 1330 1330
Size: 3953
BstBI TT'CG_AA
Cuts at: 2314 2314
Size: 3953
BstXI CCAn_nnnn'nTGG
Cuts at: 345 915 345
Size: 570 3383
BstYI r'GATC_y
Cuts at: 1 820 978 1445 1468 1803 2049 3061 3147
Size: 819 158 467 23 335 246 1012 86
Cuts at: 3147 3159 3505 1
Size: 12 346 449
Fragments arranged by size:
1012 819 467 449 346 335 246 158
86 23 12
Bsu36I CC'TnA_GG
Cuts at: 119 119
Size: 3953
BtgZI GCGATGnnnnnnnnnn'nnnn_
Cuts at: 1903 2084 2247 1903
Size: 181 163 3609
Fragments arranged by size:
3609 181 163
BtsI GCAGTG_nn'
Cuts at: 1196 1196
Size: 3953
DraI TTT'AAA
Cuts at: 952 952
Size: 3953
DraIII CAC_nnn'GTG
Cuts at: 283 283
Size: 3953
DrdI GACnn_nn'nnGTC
Cuts at: 1659 2517 1659
Size: 858 3095
EaeI y'GGCC_r
Cuts at: 746 781 869 1538 1712 2103 2130 3475 746
Size: 35 88 669 174 391 27 1345 1224
Fragments arranged by size:
1345 1224 669 391 174 88 35 27
EagI C'GGCC_G
Cuts at: 781 869 1538 781
Size: 88 669 3196
Fragments arranged by size:
3196 669 88
EarI CTCTTCn'nnn_
Cuts at: 1976 2186 1976
Size: 210 3743
EciI GGCGGAnnnnnnnnn_nn'
Cuts at: 673 2161 2471 2617 3763 673
Size: 1488 310 146 1146 863
Fragments arranged by size:
1488 1146 863 310 146
EcoICRI GAG'CTC
Cuts at: 965 965
Size: 3953
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 1778 2210 2957 3225 1778
Size: 432 747 268 2506
Fragments arranged by size:
2506 747 432 268
EcoNI CCTnn'n_nnAGG
Cuts at: 529 529
Size: 3953
EcoO109I rG'GnC_Cy
Cuts at: 139 221 314 463 754 795 1092 139
Size: 82 93 149 291 41 297 3000
Fragments arranged by size:
3000 297 291 149 93 82 41
EcoRI G'AATT_C
Cuts at: 957 957
Size: 3953
FalI AAGnnnnnCTTnnnnnnnn_nnnnn'
Cuts at: 1972 1972
Size: 3953
FspI TGC'GCA
Cuts at: 1734 1734
Size: 3953
HaeII r_GCGC'y
Cuts at: 190 588 614 812 1387 1635 2657 3636 190
Size: 398 26 198 575 248 1022 979 507
Fragments arranged by size:
1022 979 575 507 398 248 198 26
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 1795 1825 1827 1857 1795
Size: 30 2 30 3891
Fragments arranged by size:
3891 30 30 2
HincII GTy'rAC
Cuts at: 606 992 606
Size: 386 3567
HpaI GTT'AAC
Cuts at: 606 606
Size: 3953
KasI G'GCGC_C
Cuts at: 610 808 1383 1631 610
Size: 198 575 248 2932
Fragments arranged by size:
2932 575 248 198
KpnI G_GTAC'C
Cuts at: 973 973
Size: 3953
MluI A'CGCG_T
Cuts at: 3386 3386
Size: 3953
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 2624 2808 2624
Size: 184 3769
MscI TGG'CCA
Cuts at: 1714 1714
Size: 3953
MslI CAynn'nnrTG
Cuts at: 913 1942 2069 913
Size: 1029 127 2797
Fragments arranged by size:
2797 1029 127
MspA1I CmG'CkG
Cuts at: 366 1064 1378 1738 2751 2996 366
Size: 698 314 360 1013 245 1323
Fragments arranged by size:
1323 1013 698 360 314 245
NaeI GCC'GGC
Cuts at: 2134 2134
Size: 3953
NarI GG'CG_CC
Cuts at: 611 809 1384 1632 611
Size: 198 575 248 2932
Fragments arranged by size:
2932 575 248 198
NgoMIV G'CCGG_C
Cuts at: 2132 2132
Size: 3953
NspI r_CATG'y
Cuts at: 1006 1506 2413 1006
Size: 500 907 2546
Fragments arranged by size:
2546 907 500
PasI CC'CwG_GG
Cuts at: 319 384 319
Size: 65 3888
PciI A'CATG_T
Cuts at: 2409 2409
Size: 3953
PflMI CCAn_nnn'nTGG
Cuts at: 552 552
Size: 3953
PmeI GTTT'AAAC
Cuts at: 952 952
Size: 3953
PpiI GAACnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 3092 3124 3092
Size: 32 3921
PpuMI rG'GwC_Cy
Cuts at: 463 795 463
Size: 332 3621
PspOMI G'GGCC_C
Cuts at: 138 220 138
Size: 82 3871
PstI C_TGCA'G
Cuts at: 1000 1685 1000
Size: 685 3268
PvuI CG_AT'CG
Cuts at: 104 104
Size: 3953
PvuII CAG'CTG
Cuts at: 1378 1738 1378
Size: 360 3593
RsrII CG'GwC_CG
Cuts at: 2148 2148
Size: 3953
SacI G_AGCT'C
Cuts at: 967 967
Size: 3953
SalI G'TCGA_C
Cuts at: 990 990
Size: 3953
SanDI GG'GwC_CC
Cuts at: 795 795
Size: 3953
SapI GCTCTTCn'nnn_
Cuts at: 1976 2186 1976
Size: 210 3743
SbfI CC_TGCA'GG
Cuts at: 1000 1000
Size: 3953
ScaI AGT'ACT
Cuts at: 3470 3470
Size: 3953
SexAI A'CCwGG_T
Cuts at: 279 279
Size: 3953
SfcI C'TryA_G
Cuts at: 33 996 1681 2674 2865 33
Size: 963 685 993 191 1121
Fragments arranged by size:
1121 993 963 685 191
SfoI GGC'GCC
Cuts at: 612 810 1385 1633 612
Size: 198 575 248 2932
Fragments arranged by size:
2932 575 248 198
SgfI GCG_AT'CGC
Cuts at: 104 104
Size: 3953
SmaI CCC'GGG
Cuts at: 768 975 768
Size: 207 3746
SmlI C'TyrA_G
Cuts at: 1108 2023 2515 2777 1108
Size: 915 492 262 2284
Fragments arranged by size:
2284 915 492 262
SpeI A'CTAG_T
Cuts at: 67 67
Size: 3953
SphI G_CATG'C
Cuts at: 1006 1506 1006
Size: 500 3453
StyI C'CwwG_G
Cuts at: 1087 1441 1087
Size: 354 3599
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 1536 1669 2128 2337 1536
Size: 133 459 209 3152
Fragments arranged by size:
3152 459 209 133
TatI w'GTAC_w
Cuts at: 698 3468 698
Size: 2770 1183
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 3470 3502 3470
Size: 32 3921
Tth111I GACn'n_nGTC
Cuts at: 1750 1750
Size: 3953
XbaI T'CTAG_A
Cuts at: 984 984
Size: 3953
XcmI CCAnnnn_n'nnnnTGG
Cuts at: 342 1448 342
Size: 1106 2847
XmaI C'CCGG_G
Cuts at: 766 973 766
Size: 207 3746
Enzymes that do cut:
AarI AccI Acc65I AclI AcuI AfeI AflIII AgeI
AlwNI ApaI ApaLI ApoI AseI AsiSI AvaI BamHI
BanI BanII BbeI BbvCI BcgI BciVI BclI BglI
BglII BlpI Bme1580I BmrI BplI Bpu10I BpuEI BsaI
BsaAI BsaBI BsaHI BsaWI BseRI BseYI BsgI BsiEI
BsiHKAI Bsp1286I BspEI BspHI BspMI BsrBI BsrDI BsrFI
BssHII BssSI BstAPI BstBI BstXI BstYI Bsu36I BtgZI
BtsI DraI DraIII DrdI EaeI EagI EarI EciI
EcoICRI Eco57MI EcoNI EcoO109I EcoRI FalI FspI HaeII
Hin4I HincII HpaI KasI KpnI MluI MmeI MscI
MslI MspA1I NaeI NarI NgoMIV NspI PasI PciI
PflMI PmeI PpiI PpuMI PspOMI PstI PvuI PvuII
RsrII SacI SalI SanDI SapI SbfI ScaI SexAI
SfcI SfoI SgfI SmaI SmlI SpeI SphI StyI
TaqII TatI TstI Tth111I XbaI XcmI XmaI
Enzymes that do not cut:
AatII AflII AhdI AleI AlfI AloI AscI AvrII
BaeI BbsI BfrBI BmgBI BmtI BpmI BsaXI BsiWI
BsmI BsmBI BsrGI BstEII BstZ17I BtgI ClaI CspCI
EcoRV FseI FspAI HindIII MfeI NcoI NdeI NheI
NotI NruI NsiI PacI PfoI PmlI PshAI PsiI
PspXI PsrI SacII SfiI SgrAI SnaBI SrfI SspI
StuI SwaI XhoI XmnI ZraI