Restriction map for
pF1KB7570
(Circular) (Six-base) MAPSORT of: pF1KB7570.seq Check: 9899 from: 1 to: 3950
pF1KB7570 3950 bp
With 182 enzymes: SgfI *
October 7, 2009 14:23 ..
AarI CACCTGCnnnn'nnnn_
Cuts at: 624 624
Size: 3950
AccI GT'mk_AC
Cuts at: 988 988
Size: 3950
Acc65I G'GTAC_C
Cuts at: 966 966
Size: 3950
AclI AA'CG_TT
Cuts at: 3790 3790
Size: 3950
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 788 1775 2207 2954 3222 788
Size: 987 432 747 268 1516
Fragments arranged by size:
1516 987 747 432 268
AflIII A'CryG_T
Cuts at: 1302 2406 3383 1302
Size: 1104 977 1869
Fragments arranged by size:
1869 1104 977
AgeI A'CCGG_T
Cuts at: 2338 2338
Size: 3950
AlfI GCAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 417 417
Size: 3950
AlwNI CAG_nnn'CTG
Cuts at: 396 615 1255 2822 396
Size: 219 640 1567 1524
Fragments arranged by size:
1567 1524 640 219
ApaLI G'TGCA_C
Cuts at: 2720 2720
Size: 3950
ApoI r'AATT_y
Cuts at: 559 954 3303 3559 559
Size: 395 2349 256 950
Fragments arranged by size:
2349 950 395 256
AseI AT'TA_AT
Cuts at: 20 20
Size: 3950
AsiSI GCG_AT'CGC
Cuts at: 104 104
Size: 3950
AvaI C'yCGr_G
Cuts at: 378 970 378
Size: 592 3358
BamHI G'GATC_C
Cuts at: 975 975
Size: 3950
BanI G'GyrC_C
Cuts at: 966 1380 1628 1663 966
Size: 414 248 35 3253
Fragments arranged by size:
3253 414 248 35
BanII G_rGCy'C
Cuts at: 139 298 815 836 964 1994 139
Size: 159 517 21 128 1030 2095
Fragments arranged by size:
2095 1030 517 159 128 21
BbeI G_GCGC'C
Cuts at: 1384 1632 1384
Size: 248 3702
BbsI GAAGACnn'nnnn_
Cuts at: 646 646
Size: 3950
BbvCI CC'TCA_GC
Cuts at: 127 431 127
Size: 304 3646
BcgI CGAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 90 124 90
Size: 34 3916
BciVI GTATCCnnnnn_n'
Cuts at: 1848 2609 3950 1848
Size: 761 1341 1848
Fragments arranged by size:
1848 1341 761
BclI T'GATC_A
Cuts at: 332 1470 332
Size: 1138 2812
BglII A'GATC_T
Cuts at: 1465 3502 1465
Size: 2037 1913
BlpI GC'TnA_GC
Cuts at: 133 1062 133
Size: 929 3021
Bme1580I G_kGCm'C
Cuts at: 253 1575 1668 2724 253
Size: 1322 93 1056 1479
Fragments arranged by size:
1479 1322 1056 93
BmrI ACTGGGnnnn_n'
Cuts at: 1162 1243 1578 3722 1162
Size: 81 335 2144 1390
Fragments arranged by size:
2144 1390 335 81
BplI GAGnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 216 441 2317 216
Size: 225 1876 1849
Fragments arranged by size:
1876 1849 225
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 571 829 571
Size: 258 3692
Bpu10I CC'TnA_GC
Cuts at: 127 431 127
Size: 304 3646
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 1126 2005 2497 2795 1126
Size: 879 492 298 2281
Fragments arranged by size:
2281 879 492 298
BsaI GGTCTCn'nnnn_
Cuts at: 202 429 3654 202
Size: 227 3225 498
Fragments arranged by size:
3225 498 227
BsaAI yAC'GTr
Cuts at: 862 1303 862
Size: 441 3509
BsaBI GATnn'nnATC
Cuts at: 6 1464 3501 6
Size: 1458 2037 455
Fragments arranged by size:
2037 1458 455
BsaHI Gr'CG_yC
Cuts at: 1381 1629 2331 3827 1381
Size: 248 702 1496 1504
Fragments arranged by size:
1504 1496 702 248
BsaWI w'CCGG_w
Cuts at: 1139 1660 2338 2612 2759 3170 1139
Size: 521 678 274 147 411 1919
Fragments arranged by size:
1919 678 521 411 274 147
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 313 844 313
Size: 531 3419
BseYI C'CCAG_C
Cuts at: 1375 2133 2710 3206 1375
Size: 758 577 496 2119
Fragments arranged by size:
2119 758 577 496
BsiEI CG_ry'CG
Cuts at: 104 1492 1538 2746 104
Size: 1388 46 1208 1308
Fragments arranged by size:
1388 1308 1208 46
BsiHKAI G_wGCw'C
Cuts at: 139 836 964 1742 1932 2724 139
Size: 697 128 778 190 792 1365
Fragments arranged by size:
1365 792 778 697 190 128
BsmI GAATG_Cn'
Cuts at: 282 282
Size: 3950
BsmBI CGTCTCn'nnnn_
Cuts at: 466 466
Size: 3950
Bsp1286I G_dGCh'C
Cuts at: 139 253 298 815 836 964 1575 1668 1742
Size: 114 45 517 21 128 611 93 74
Cuts at: 1742 1932 1994 2724 139
Size: 190 62 730 1365
Fragments arranged by size:
1365 730 611 517 190 128 114 93
74 62 45 21
BspEI T'CCGG_A
Cuts at: 3170 3170
Size: 3950
BspHI T'CATG_A
Cuts at: 182 3126 182
Size: 2944 1006
BspMI ACCTGCnnnn'nnnn_
Cuts at: 220 624 1000 1214 1516 1897 220
Size: 404 376 214 302 381 2273
Fragments arranged by size:
2273 404 381 376 302 214
BsrBI CCG'CTC
Cuts at: 50 321 2242 2296 3780 3945 50
Size: 271 1921 54 1484 165 55
Fragments arranged by size:
1921 1484 271 165 55 54
BsrDI GCAATG_nn'
Cuts at: 1862 1862
Size: 3950
BsrFI r'CCGG_y
Cuts at: 1948 2129 2338 1948
Size: 181 209 3560
Fragments arranged by size:
3560 209 181
BssSI C'ACGA_G
Cuts at: 2221 2579 2221
Size: 358 3592
BstAPI GCAn_nnn'nTGC
Cuts at: 1327 1327
Size: 3950
BstBI TT'CG_AA
Cuts at: 2311 2311
Size: 3950
BstEII G'GTnAC_C
Cuts at: 411 411
Size: 3950
BstYI r'GATC_y
Cuts at: 1 194 975 1442 1465 1800 2046 3058 3144
Size: 193 781 467 23 335 246 1012 86
Cuts at: 3144 3156 3502 1
Size: 12 346 449
Fragments arranged by size:
1012 781 467 449 346 335 246 193
86 23 12
BtgZI GCGATGnnnnnnnnnn'nnnn_
Cuts at: 1900 2081 2244 1900
Size: 181 163 3606
Fragments arranged by size:
3606 181 163
BtsI GCAGTG_nn'
Cuts at: 111 1193 111
Size: 1082 2868
DraI TTT'AAA
Cuts at: 949 949
Size: 3950
DrdI GACnn_nn'nnGTC
Cuts at: 1656 2514 1656
Size: 858 3092
EaeI y'GGCC_r
Cuts at: 144 1535 1709 2100 2127 3472 144
Size: 1391 174 391 27 1345 622
Fragments arranged by size:
1391 1345 622 391 174 27
EagI C'GGCC_G
Cuts at: 1535 1535
Size: 3950
EarI CTCTTCn'nnn_
Cuts at: 749 764 927 1973 2183 749
Size: 15 163 1046 210 2516
Fragments arranged by size:
2516 1046 210 163 15
EciI GGCGGAnnnnnnnnn_nn'
Cuts at: 2158 2468 2614 3760 2158
Size: 310 146 1146 2348
Fragments arranged by size:
2348 1146 310 146
EcoICRI GAG'CTC
Cuts at: 137 834 962 137
Size: 697 128 3125
Fragments arranged by size:
3125 697 128
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 571 788 829 1775 2207 2954 3222 571
Size: 217 41 946 432 747 268 1299
Fragments arranged by size:
1299 946 747 432 268 217 41
EcoNI CCTnn'n_nnAGG
Cuts at: 202 202
Size: 3950
EcoO109I rG'GnC_Cy
Cuts at: 435 450 1089 435
Size: 15 639 3296
Fragments arranged by size:
3296 639 15
EcoRI G'AATT_C
Cuts at: 954 954
Size: 3950
FalI AAGnnnnnCTTnnnnnnnn_nnnnn'
Cuts at: 1969 1969
Size: 3950
FspI TGC'GCA
Cuts at: 1731 1731
Size: 3950
HaeII r_GCGC'y
Cuts at: 171 1384 1632 2654 3633 171
Size: 1213 248 1022 979 488
Fragments arranged by size:
1213 1022 979 488 248
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 633 665 1792 1822 1824 1854 633
Size: 32 1127 30 2 30 2729
Fragments arranged by size:
2729 1127 32 30 30 2
HincII GTy'rAC
Cuts at: 584 989 584
Size: 405 3545
KasI G'GCGC_C
Cuts at: 1380 1628 1380
Size: 248 3702
KpnI G_GTAC'C
Cuts at: 970 970
Size: 3950
MluI A'CGCG_T
Cuts at: 3383 3383
Size: 3950
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 578 2621 2805 578
Size: 2043 184 1723
Fragments arranged by size:
2043 1723 184
MscI TGG'CCA
Cuts at: 1711 1711
Size: 3950
MslI CAynn'nnrTG
Cuts at: 1939 2066 1939
Size: 127 3823
MspA1I CmG'CkG
Cuts at: 630 807 1061 1375 1735 2748 2993 630
Size: 177 254 314 360 1013 245 1587
Fragments arranged by size:
1587 1013 360 314 254 245 177
NaeI GCC'GGC
Cuts at: 2131 2131
Size: 3950
NarI GG'CG_CC
Cuts at: 1381 1629 1381
Size: 248 3702
NgoMIV G'CCGG_C
Cuts at: 2129 2129
Size: 3950
NspI r_CATG'y
Cuts at: 1003 1503 2410 1003
Size: 500 907 2543
Fragments arranged by size:
2543 907 500
PasI CC'CwG_GG
Cuts at: 439 792 439
Size: 353 3597
PciI A'CATG_T
Cuts at: 2406 2406
Size: 3950
PmeI GTTT'AAAC
Cuts at: 949 949
Size: 3950
PmlI CAC'GTG
Cuts at: 862 862
Size: 3950
PpiI GAACnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 3089 3121 3089
Size: 32 3918
PstI C_TGCA'G
Cuts at: 539 635 997 1682 539
Size: 96 362 685 2807
Fragments arranged by size:
2807 685 362 96
PvuI CG_AT'CG
Cuts at: 104 104
Size: 3950
PvuII CAG'CTG
Cuts at: 630 807 1375 1735 630
Size: 177 568 360 2845
Fragments arranged by size:
2845 568 360 177
RsrII CG'GwC_CG
Cuts at: 2145 2145
Size: 3950
SacI G_AGCT'C
Cuts at: 139 836 964 139
Size: 697 128 3125
Fragments arranged by size:
3125 697 128
SalI G'TCGA_C
Cuts at: 987 987
Size: 3950
SapI GCTCTTCn'nnn_
Cuts at: 1973 2183 1973
Size: 210 3740
SbfI CC_TGCA'GG
Cuts at: 997 997
Size: 3950
ScaI AGT'ACT
Cuts at: 3467 3467
Size: 3950
SexAI A'CCwGG_T
Cuts at: 362 407 643 362
Size: 45 236 3669
Fragments arranged by size:
3669 236 45
SfcI C'TryA_G
Cuts at: 33 535 631 993 1678 2671 2862 33
Size: 502 96 362 685 993 191 1121
Fragments arranged by size:
1121 993 685 502 362 191 96
SfoI GGC'GCC
Cuts at: 1382 1630 1382
Size: 248 3702
SgfI GCG_AT'CGC
Cuts at: 104 104
Size: 3950
SmaI CCC'GGG
Cuts at: 380 972 380
Size: 592 3358
SmlI C'TyrA_G
Cuts at: 1105 2020 2512 2774 1105
Size: 915 492 262 2281
Fragments arranged by size:
2281 915 492 262
SpeI A'CTAG_T
Cuts at: 67 67
Size: 3950
SphI G_CATG'C
Cuts at: 1003 1503 1003
Size: 500 3450
StyI C'CwwG_G
Cuts at: 203 1084 1438 203
Size: 881 354 2715
Fragments arranged by size:
2715 881 354
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 720 1533 1666 2125 2334 720
Size: 813 133 459 209 2336
Fragments arranged by size:
2336 813 459 209 133
TatI w'GTAC_w
Cuts at: 3465 3465
Size: 3950
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 851 883 3467 3499 851
Size: 32 2584 32 1302
Fragments arranged by size:
2584 1302 32 32
Tth111I GACn'n_nGTC
Cuts at: 1747 1747
Size: 3950
XbaI T'CTAG_A
Cuts at: 981 981
Size: 3950
XcmI CCAnnnn_n'nnnnTGG
Cuts at: 1445 1445
Size: 3950
XmaI C'CCGG_G
Cuts at: 378 970 378
Size: 592 3358
XmnI GAAnn'nnTTC
Cuts at: 559 559
Size: 3950
Enzymes that do cut:
AarI AccI Acc65I AclI AcuI AflIII AgeI AlfI
AlwNI ApaLI ApoI AseI AsiSI AvaI BamHI BanI
BanII BbeI BbsI BbvCI BcgI BciVI BclI BglII
BlpI Bme1580I BmrI BplI BpmI Bpu10I BpuEI BsaI
BsaAI BsaBI BsaHI BsaWI BseRI BseYI BsiEI BsiHKAI
BsmI BsmBI Bsp1286I BspEI BspHI BspMI BsrBI BsrDI
BsrFI BssSI BstAPI BstBI BstEII BstYI BtgZI BtsI
DraI DrdI EaeI EagI EarI EciI EcoICRI Eco57MI
EcoNI EcoO109I EcoRI FalI FspI HaeII Hin4I HincII
KasI KpnI MluI MmeI MscI MslI MspA1I NaeI
NarI NgoMIV NspI PasI PciI PmeI PmlI PpiI
PstI PvuI PvuII RsrII SacI SalI SapI SbfI
ScaI SexAI SfcI SfoI SgfI SmaI SmlI SpeI
SphI StyI TaqII TatI TstI Tth111I XbaI XcmI
XmaI XmnI
Enzymes that do not cut:
AatII AfeI AflII AhdI AleI AloI ApaI AscI
AvrII BaeI BfrBI BglI BmgBI BmtI BsaXI BsgI
BsiWI BsrGI BssHII BstXI BstZ17I Bsu36I BtgI ClaI
CspCI DraIII EcoRV FseI FspAI HindIII HpaI MfeI
NcoI NdeI NheI NotI NruI NsiI PacI PflMI
PfoI PpuMI PshAI PsiI PspOMI PspXI PsrI SacII
SanDI SfiI SgrAI SnaBI SrfI SspI StuI SwaI
XhoI ZraI