Restriction map for
pF1KB7552
(Circular) (Six-base) MAPSORT of: pF1KB7552.seq Check: 4800 from: 1 to: 3917
pF1KB7552 3917 bp
With 182 enzymes: SgfI *
June 20, 2008 10:27 ..
AccI GT'mk_AC
Cuts at: 955 955
Size: 3917
Acc65I G'GTAC_C
Cuts at: 933 933
Size: 3917
AclI AA'CG_TT
Cuts at: 3757 3757
Size: 3917
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 649 1742 2174 2921 3189 649
Size: 1093 432 747 268 1377
Fragments arranged by size:
1377 1093 747 432 268
AflIII A'CryG_T
Cuts at: 909 1269 2373 3350 909
Size: 360 1104 977 1476
Fragments arranged by size:
1476 1104 977 360
AgeI A'CCGG_T
Cuts at: 2305 2305
Size: 3917
AlfI GCAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 578 578
Size: 3917
AlwNI CAG_nnn'CTG
Cuts at: 698 1222 2789 698
Size: 524 1567 1826
Fragments arranged by size:
1826 1567 524
ApaLI G'TGCA_C
Cuts at: 2687 2687
Size: 3917
ApoI r'AATT_y
Cuts at: 921 3270 3526 921
Size: 2349 256 1312
Fragments arranged by size:
2349 1312 256
AseI AT'TA_AT
Cuts at: 20 20
Size: 3917
AsiSI GCG_AT'CGC
Cuts at: 104 104
Size: 3917
AvaI C'yCGr_G
Cuts at: 937 937
Size: 3917
BamHI G'GATC_C
Cuts at: 942 942
Size: 3917
BanI G'GyrC_C
Cuts at: 622 933 1347 1595 1630 622
Size: 311 414 248 35 2909
Fragments arranged by size:
2909 414 311 248 35
BanII G_rGCy'C
Cuts at: 798 931 1961 798
Size: 133 1030 2754
Fragments arranged by size:
2754 1030 133
BbeI G_GCGC'C
Cuts at: 626 1351 1599 626
Size: 725 248 2944
Fragments arranged by size:
2944 725 248
BbvCI CC'TCA_GC
Cuts at: 250 250
Size: 3917
BcgI CGAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 90 124 90
Size: 34 3883
BciVI GTATCCnnnnn_n'
Cuts at: 334 1815 2576 3917 334
Size: 1481 761 1341 334
Fragments arranged by size:
1481 1341 761 334
BclI T'GATC_A
Cuts at: 1437 1437
Size: 3917
BfrBI ATG'CAT
Cuts at: 636 636
Size: 3917
BglII A'GATC_T
Cuts at: 671 1432 3469 671
Size: 761 2037 1119
Fragments arranged by size:
2037 1119 761
BlpI GC'TnA_GC
Cuts at: 1029 1029
Size: 3917
Bme1580I G_kGCm'C
Cuts at: 1542 1635 2691 1542
Size: 93 1056 2768
Fragments arranged by size:
2768 1056 93
BmrI ACTGGGnnnn_n'
Cuts at: 561 1129 1210 1545 3689 561
Size: 568 81 335 2144 789
Fragments arranged by size:
2144 789 568 335 81
BplI GAGnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 234 389 2284 234
Size: 155 1895 1867
Fragments arranged by size:
1895 1867 155
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 843 843
Size: 3917
Bpu10I CC'TnA_GC
Cuts at: 250 255 250
Size: 5 3912
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 1093 1972 2464 2762 1093
Size: 879 492 298 2248
Fragments arranged by size:
2248 879 492 298
BsaI GGTCTCn'nnnn_
Cuts at: 3621 3621
Size: 3917
BsaAI yAC'GTr
Cuts at: 1270 1270
Size: 3917
BsaBI GATnn'nnATC
Cuts at: 6 1431 3468 6
Size: 1425 2037 455
Fragments arranged by size:
2037 1425 455
BsaHI Gr'CG_yC
Cuts at: 623 1348 1596 2298 3794 623
Size: 725 248 702 1496 746
Fragments arranged by size:
1496 746 725 702 248
BsaWI w'CCGG_w
Cuts at: 337 1106 1627 2305 2579 2726 3137 337
Size: 769 521 678 274 147 411 1117
Fragments arranged by size:
1117 769 678 521 411 274 147
BsaXI ACnnnnnCTCCnnnnnnn_nnn'
Cuts at: 279 309 376 406 279
Size: 30 67 30 3790
Fragments arranged by size:
3790 67 30 30
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 320 336 365 381 320
Size: 16 29 16 3856
Fragments arranged by size:
3856 29 16 16
BseYI C'CCAG_C
Cuts at: 308 1342 2100 2677 3173 308
Size: 1034 758 577 496 1052
Fragments arranged by size:
1052 1034 758 577 496
BsgI GTGCAGnnnnnnnnnnnnnn_nn'
Cuts at: 538 859 538
Size: 321 3596
BsiEI CG_ry'CG
Cuts at: 104 1459 1505 2713 104
Size: 1355 46 1208 1308
Fragments arranged by size:
1355 1308 1208 46
BsiHKAI G_wGCw'C
Cuts at: 931 1709 1899 2691 931
Size: 778 190 792 2157
Fragments arranged by size:
2157 792 778 190
Bsp1286I G_dGCh'C
Cuts at: 798 931 1542 1635 1709 1899 1961 2691 798
Size: 133 611 93 74 190 62 730 2024
Fragments arranged by size:
2024 730 611 190 133 93 74 62
BspEI T'CCGG_A
Cuts at: 337 3137 337
Size: 2800 1117
BspHI T'CATG_A
Cuts at: 3093 3093
Size: 3917
BspMI ACCTGCnnnn'nnnn_
Cuts at: 967 1181 1483 1864 967
Size: 214 302 381 3020
Fragments arranged by size:
3020 381 302 214
BsrBI CCG'CTC
Cuts at: 50 2209 2263 3747 3912 50
Size: 2159 54 1484 165 55
Fragments arranged by size:
2159 1484 165 55 54
BsrDI GCAATG_nn'
Cuts at: 1829 1829
Size: 3917
BsrFI r'CCGG_y
Cuts at: 1915 2096 2305 1915
Size: 181 209 3527
Fragments arranged by size:
3527 209 181
BssSI C'ACGA_G
Cuts at: 2188 2546 2188
Size: 358 3559
BstAPI GCAn_nnn'nTGC
Cuts at: 503 674 842 1294 503
Size: 171 168 452 3126
Fragments arranged by size:
3126 452 171 168
BstBI TT'CG_AA
Cuts at: 2278 2278
Size: 3917
BstYI r'GATC_y
Cuts at: 1 245 671 942 1409 1432 1767 2013 3025
Size: 244 426 271 467 23 335 246 1012
Cuts at: 3025 3111 3123 3469 1
Size: 86 12 346 449
Fragments arranged by size:
1012 467 449 426 346 335 271 246
244 86 23 12
BtgZI GCGATGnnnnnnnnnn'nnnn_
Cuts at: 575 1867 2048 2211 575
Size: 1292 181 163 2281
Fragments arranged by size:
2281 1292 181 163
BtsI GCAGTG_nn'
Cuts at: 555 756 1160 555
Size: 201 404 3312
Fragments arranged by size:
3312 404 201
DraI TTT'AAA
Cuts at: 916 916
Size: 3917
DraIII CAC_nnn'GTG
Cuts at: 281 281
Size: 3917
DrdI GACnn_nn'nnGTC
Cuts at: 1623 2481 1623
Size: 858 3059
EaeI y'GGCC_r
Cuts at: 536 1502 1676 2067 2094 3439 536
Size: 966 174 391 27 1345 1014
Fragments arranged by size:
1345 1014 966 391 174 27
EagI C'GGCC_G
Cuts at: 1502 1502
Size: 3917
EarI CTCTTCn'nnn_
Cuts at: 428 1940 2150 428
Size: 1512 210 2195
Fragments arranged by size:
2195 1512 210
EciI GGCGGAnnnnnnnnn_nn'
Cuts at: 2125 2435 2581 3727 2125
Size: 310 146 1146 2315
Fragments arranged by size:
2315 1146 310 146
EcoICRI GAG'CTC
Cuts at: 929 929
Size: 3917
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 649 843 1742 2174 2921 3189 649
Size: 194 899 432 747 268 1377
Fragments arranged by size:
1377 899 747 432 268 194
EcoO109I rG'GnC_Cy
Cuts at: 1056 1056
Size: 3917
EcoRI G'AATT_C
Cuts at: 921 921
Size: 3917
FalI AAGnnnnnCTTnnnnnnnn_nnnnn'
Cuts at: 1936 1936
Size: 3917
FspI TGC'GCA
Cuts at: 1698 1698
Size: 3917
HaeII r_GCGC'y
Cuts at: 626 1351 1599 2621 3600 626
Size: 725 248 1022 979 943
Fragments arranged by size:
1022 979 943 725 248
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 376 408 553 585 783 815 1759 1789 1791
Size: 32 145 32 198 32 944 30 2
Cuts at: 1791 1821 376
Size: 30 2472
Fragments arranged by size:
2472 944 198 145 32 32 32 30
30 2
HincII GTy'rAC
Cuts at: 956 956
Size: 3917
HindIII A'AGCT_T
Cuts at: 454 454
Size: 3917
KasI G'GCGC_C
Cuts at: 622 1347 1595 622
Size: 725 248 2944
Fragments arranged by size:
2944 725 248
KpnI G_GTAC'C
Cuts at: 937 937
Size: 3917
MluI A'CGCG_T
Cuts at: 3350 3350
Size: 3917
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 2588 2772 2588
Size: 184 3733
MscI TGG'CCA
Cuts at: 538 1678 538
Size: 1140 2777
MslI CAynn'nnrTG
Cuts at: 277 560 815 881 1906 2033 277
Size: 283 255 66 1025 127 2161
Fragments arranged by size:
2161 1025 283 255 127 66
MspA1I CmG'CkG
Cuts at: 292 552 587 1028 1342 1702 2715 2960 292
Size: 260 35 441 314 360 1013 245 1249
Fragments arranged by size:
1249 1013 441 360 314 260 245 35
NaeI GCC'GGC
Cuts at: 2098 2098
Size: 3917
NarI GG'CG_CC
Cuts at: 623 1348 1596 623
Size: 725 248 2944
Fragments arranged by size:
2944 725 248
NgoMIV G'CCGG_C
Cuts at: 2096 2096
Size: 3917
NsiI A_TGCA'T
Cuts at: 638 638
Size: 3917
NspI r_CATG'y
Cuts at: 970 1470 2377 970
Size: 500 907 2510
Fragments arranged by size:
2510 907 500
PciI A'CATG_T
Cuts at: 2373 2373
Size: 3917
PflMI CCAn_nnn'nTGG
Cuts at: 698 698
Size: 3917
PmeI GTTT'AAAC
Cuts at: 916 916
Size: 3917
PpiI GAACnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 3056 3088 3056
Size: 32 3885
PshAI GACnn'nnGTC
Cuts at: 447 447
Size: 3917
PstI C_TGCA'G
Cuts at: 806 964 1649 806
Size: 158 685 3074
Fragments arranged by size:
3074 685 158
PvuI CG_AT'CG
Cuts at: 104 104
Size: 3917
PvuII CAG'CTG
Cuts at: 552 587 1342 1702 552
Size: 35 755 360 2767
Fragments arranged by size:
2767 755 360 35
RsrII CG'GwC_CG
Cuts at: 2112 2112
Size: 3917
SacI G_AGCT'C
Cuts at: 931 931
Size: 3917
SalI G'TCGA_C
Cuts at: 954 954
Size: 3917
SapI GCTCTTCn'nnn_
Cuts at: 1940 2150 1940
Size: 210 3707
SbfI CC_TGCA'GG
Cuts at: 964 964
Size: 3917
ScaI AGT'ACT
Cuts at: 3434 3434
Size: 3917
SfcI C'TryA_G
Cuts at: 33 162 445 802 960 1645 2638 2829 33
Size: 129 283 357 158 685 993 191 1121
Fragments arranged by size:
1121 993 685 357 283 191 158 129
SfoI GGC'GCC
Cuts at: 624 1349 1597 624
Size: 725 248 2944
Fragments arranged by size:
2944 725 248
SgfI GCG_AT'CGC
Cuts at: 104 104
Size: 3917
SmaI CCC'GGG
Cuts at: 939 939
Size: 3917
SmlI C'TyrA_G
Cuts at: 1072 1987 2479 2741 1072
Size: 915 492 262 2248
Fragments arranged by size:
2248 915 492 262
SpeI A'CTAG_T
Cuts at: 67 67
Size: 3917
SphI G_CATG'C
Cuts at: 970 1470 970
Size: 500 3417
StyI C'CwwG_G
Cuts at: 395 1051 1405 395
Size: 656 354 2907
Fragments arranged by size:
2907 656 354
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 1500 1633 2092 2301 1500
Size: 133 459 209 3116
Fragments arranged by size:
3116 459 209 133
TatI w'GTAC_w
Cuts at: 3432 3432
Size: 3917
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 3434 3466 3434
Size: 32 3885
Tth111I GACn'n_nGTC
Cuts at: 1714 1714
Size: 3917
XbaI T'CTAG_A
Cuts at: 571 948 571
Size: 377 3540
XcmI CCAnnnn_n'nnnnTGG
Cuts at: 316 1412 316
Size: 1096 2821
XmaI C'CCGG_G
Cuts at: 937 937
Size: 3917
XmnI GAAnn'nnTTC
Cuts at: 136 488 136
Size: 352 3565
Enzymes that do cut:
AccI Acc65I AclI AcuI AflIII AgeI AlfI AlwNI
ApaLI ApoI AseI AsiSI AvaI BamHI BanI BanII
BbeI BbvCI BcgI BciVI BclI BfrBI BglII BlpI
Bme1580I BmrI BplI BpmI Bpu10I BpuEI BsaI BsaAI
BsaBI BsaHI BsaWI BsaXI BseRI BseYI BsgI BsiEI
BsiHKAI Bsp1286I BspEI BspHI BspMI BsrBI BsrDI BsrFI
BssSI BstAPI BstBI BstYI BtgZI BtsI DraI DraIII
DrdI EaeI EagI EarI EciI EcoICRI Eco57MI EcoO109I
EcoRI FalI FspI HaeII Hin4I HincII HindIII KasI
KpnI MluI MmeI MscI MslI MspA1I NaeI NarI
NgoMIV NsiI NspI PciI PflMI PmeI PpiI PshAI
PstI PvuI PvuII RsrII SacI SalI SapI SbfI
ScaI SfcI SfoI SgfI SmaI SmlI SpeI SphI
StyI TaqII TatI TstI Tth111I XbaI XcmI XmaI
XmnI
Enzymes that do not cut:
AarI AatII AfeI AflII AhdI AleI AloI ApaI
AscI AvrII BaeI BbsI BglI BmgBI BmtI BsiWI
BsmI BsmBI BsrGI BssHII BstEII BstXI BstZ17I Bsu36I
BtgI ClaI CspCI EcoNI EcoRV FseI FspAI HpaI
MfeI NcoI NdeI NheI NotI NruI PacI PasI
PfoI PmlI PpuMI PsiI PspOMI PspXI PsrI SacII
SanDI SexAI SfiI SgrAI SnaBI SrfI SspI StuI
SwaI XhoI ZraI