Restriction map for
pF1KB7508
(Circular) (Six-base) MAPSORT of: pF1KB7508.seq Check: 7599 from: 1 to: 3839
pF1KB7508 3839 bp
With 182 enzymes: SgfI *
March 2, 2010 14:27 ..
AarI CACCTGCnnnn'nnnn_
Cuts at: 661 661
Size: 3839
AccI GT'mk_AC
Cuts at: 877 877
Size: 3839
Acc65I G'GTAC_C
Cuts at: 223 855 223
Size: 632 3207
AclI AA'CG_TT
Cuts at: 3679 3679
Size: 3839
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 475 585 1664 2096 2843 3111 475
Size: 110 1079 432 747 268 1203
Fragments arranged by size:
1203 1079 747 432 268 110
AflIII A'CryG_T
Cuts at: 288 1191 2295 3272 288
Size: 903 1104 977 855
Fragments arranged by size:
1104 977 903 855
AgeI A'CCGG_T
Cuts at: 2227 2227
Size: 3839
AloI GAACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 788 820 788
Size: 32 3807
AlwNI CAG_nnn'CTG
Cuts at: 339 489 546 585 1144 2711 339
Size: 150 57 39 559 1567 1467
Fragments arranged by size:
1567 1467 559 150 57 39
ApaLI G'TGCA_C
Cuts at: 2609 2609
Size: 3839
ApoI r'AATT_y
Cuts at: 843 3192 3448 843
Size: 2349 256 1234
Fragments arranged by size:
2349 1234 256
AseI AT'TA_AT
Cuts at: 20 20
Size: 3839
AsiSI GCG_AT'CGC
Cuts at: 104 104
Size: 3839
AvaI C'yCGr_G
Cuts at: 303 744 859 303
Size: 441 115 3283
Fragments arranged by size:
3283 441 115
BamHI G'GATC_C
Cuts at: 864 864
Size: 3839
BanI G'GyrC_C
Cuts at: 223 297 855 1269 1517 1552 223
Size: 74 558 414 248 35 2510
Fragments arranged by size:
2510 558 414 248 74 35
BanII G_rGCy'C
Cuts at: 698 853 1883 698
Size: 155 1030 2654
Fragments arranged by size:
2654 1030 155
BbeI G_GCGC'C
Cuts at: 301 1273 1521 301
Size: 972 248 2619
Fragments arranged by size:
2619 972 248
BciVI GTATCCnnnnn_n'
Cuts at: 218 1737 2498 3839 218
Size: 1519 761 1341 218
Fragments arranged by size:
1519 1341 761 218
BclI T'GATC_A
Cuts at: 1359 1359
Size: 3839
BglI GCCn_nnn'nGGC
Cuts at: 448 448
Size: 3839
BglII A'GATC_T
Cuts at: 1354 3391 1354
Size: 2037 1802
BlpI GC'TnA_GC
Cuts at: 951 951
Size: 3839
Bme1580I G_kGCm'C
Cuts at: 1464 1557 2613 1464
Size: 93 1056 2690
Fragments arranged by size:
2690 1056 93
BmrI ACTGGGnnnn_n'
Cuts at: 657 1051 1132 1467 3611 657
Size: 394 81 335 2144 885
Fragments arranged by size:
2144 885 394 335 81
BplI GAGnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 2206 2206
Size: 3839
Bpu10I CC'TnA_GC
Cuts at: 710 710
Size: 3839
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 649 1015 1894 2386 2684 649
Size: 366 879 492 298 1804
Fragments arranged by size:
1804 879 492 366 298
BsaI GGTCTCn'nnnn_
Cuts at: 3543 3543
Size: 3839
BsaAI yAC'GTr
Cuts at: 1192 1192
Size: 3839
BsaBI GATnn'nnATC
Cuts at: 6 1353 3390 6
Size: 1347 2037 455
Fragments arranged by size:
2037 1347 455
BsaHI Gr'CG_yC
Cuts at: 298 1270 1518 2220 3716 298
Size: 972 248 702 1496 421
Fragments arranged by size:
1496 972 702 421 248
BsaWI w'CCGG_w
Cuts at: 1028 1549 2227 2501 2648 3059 1028
Size: 521 678 274 147 411 1808
Fragments arranged by size:
1808 678 521 411 274 147
BsaXI ACnnnnnCTCCnnnnnnn_nnn'
Cuts at: 207 237 788 818 207
Size: 30 551 30 3228
Fragments arranged by size:
3228 551 30 30
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 432 734 432
Size: 302 3537
BseYI C'CCAG_C
Cuts at: 585 1264 2022 2599 3095 585
Size: 679 758 577 496 1329
Fragments arranged by size:
1329 758 679 577 496
BsgI GTGCAGnnnnnnnnnnnnnn_nn'
Cuts at: 535 535
Size: 3839
BsiEI CG_ry'CG
Cuts at: 104 1381 1427 2635 104
Size: 1277 46 1208 1308
Fragments arranged by size:
1308 1277 1208 46
BsiHKAI G_wGCw'C
Cuts at: 853 1631 1821 2613 853
Size: 778 190 792 2079
Fragments arranged by size:
2079 792 778 190
Bsp1286I G_dGCh'C
Cuts at: 698 853 1464 1557 1631 1821 1883 2613 698
Size: 155 611 93 74 190 62 730 1924
Fragments arranged by size:
1924 730 611 190 155 93 74 62
BspEI T'CCGG_A
Cuts at: 3059 3059
Size: 3839
BspHI T'CATG_A
Cuts at: 3015 3015
Size: 3839
BspMI ACCTGCnnnn'nnnn_
Cuts at: 367 554 661 889 1103 1405 1786 367
Size: 187 107 228 214 302 381 2420
Fragments arranged by size:
2420 381 302 228 214 187 107
BsrBI CCG'CTC
Cuts at: 50 534 2131 2185 3669 3834 50
Size: 484 1597 54 1484 165 55
Fragments arranged by size:
1597 1484 484 165 55 54
BsrDI GCAATG_nn'
Cuts at: 1751 1751
Size: 3839
BsrFI r'CCGG_y
Cuts at: 386 554 1837 2018 2227 386
Size: 168 1283 181 209 1998
Fragments arranged by size:
1998 1283 209 181 168
BssSI C'ACGA_G
Cuts at: 697 2110 2468 697
Size: 1413 358 2068
Fragments arranged by size:
2068 1413 358
BstAPI GCAn_nnn'nTGC
Cuts at: 359 546 1216 359
Size: 187 670 2982
Fragments arranged by size:
2982 670 187
BstBI TT'CG_AA
Cuts at: 2200 2200
Size: 3839
BstXI CCAn_nnnn'nTGG
Cuts at: 114 114
Size: 3839
BstYI r'GATC_y
Cuts at: 1 864 1331 1354 1689 1935 2947 3033 3045
Size: 863 467 23 335 246 1012 86 12
Cuts at: 3045 3391 1
Size: 346 449
Fragments arranged by size:
1012 863 467 449 346 335 246 86
23 12
BtgZI GCGATGnnnnnnnnnn'nnnn_
Cuts at: 1789 1970 2133 1789
Size: 181 163 3495
Fragments arranged by size:
3495 181 163
BtsI GCAGTG_nn'
Cuts at: 321 1082 321
Size: 761 3078
DraI TTT'AAA
Cuts at: 838 838
Size: 3839
DrdI GACnn_nn'nnGTC
Cuts at: 1545 2403 1545
Size: 858 2981
EaeI y'GGCC_r
Cuts at: 330 470 667 1424 1598 1989 2016 3361 330
Size: 140 197 757 174 391 27 1345 808
Fragments arranged by size:
1345 808 757 391 197 174 140 27
EagI C'GGCC_G
Cuts at: 1424 1424
Size: 3839
EarI CTCTTCn'nnn_
Cuts at: 1862 2072 1862
Size: 210 3629
EciI GGCGGAnnnnnnnnn_nn'
Cuts at: 2047 2357 2503 3649 2047
Size: 310 146 1146 2237
Fragments arranged by size:
2237 1146 310 146
EcoICRI GAG'CTC
Cuts at: 851 851
Size: 3839
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 475 585 1664 2096 2843 3111 475
Size: 110 1079 432 747 268 1203
Fragments arranged by size:
1203 1079 747 432 268 110
EcoO109I rG'GnC_Cy
Cuts at: 543 978 543
Size: 435 3404
EcoRI G'AATT_C
Cuts at: 843 843
Size: 3839
FalI AAGnnnnnCTTnnnnnnnn_nnnnn'
Cuts at: 643 1858 643
Size: 1215 2624
FspI TGC'GCA
Cuts at: 649 1620 649
Size: 971 2868
HaeII r_GCGC'y
Cuts at: 301 518 1273 1521 2543 3522 301
Size: 217 755 248 1022 979 618
Fragments arranged by size:
1022 979 755 618 248 217
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 1681 1711 1713 1743 1681
Size: 30 2 30 3777
Fragments arranged by size:
3777 30 30 2
HincII GTy'rAC
Cuts at: 878 878
Size: 3839
KasI G'GCGC_C
Cuts at: 297 1269 1517 297
Size: 972 248 2619
Fragments arranged by size:
2619 972 248
KpnI G_GTAC'C
Cuts at: 227 859 227
Size: 632 3207
MluI A'CGCG_T
Cuts at: 3272 3272
Size: 3839
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 2510 2694 2510
Size: 184 3655
MscI TGG'CCA
Cuts at: 472 669 1600 472
Size: 197 931 2711
Fragments arranged by size:
2711 931 197
MslI CAynn'nnrTG
Cuts at: 112 1828 1955 112
Size: 1716 127 1996
Fragments arranged by size:
1996 1716 127
MspA1I CmG'CkG
Cuts at: 278 950 1264 1624 2637 2882 278
Size: 672 314 360 1013 245 1235
Fragments arranged by size:
1235 1013 672 360 314 245
NaeI GCC'GGC
Cuts at: 2020 2020
Size: 3839
NarI GG'CG_CC
Cuts at: 298 1270 1518 298
Size: 972 248 2619
Fragments arranged by size:
2619 972 248
NgoMIV G'CCGG_C
Cuts at: 2018 2018
Size: 3839
NspI r_CATG'y
Cuts at: 292 892 1392 2299 292
Size: 600 500 907 1832
Fragments arranged by size:
1832 907 600 500
PciI A'CATG_T
Cuts at: 288 2295 288
Size: 2007 1832
PmeI GTTT'AAAC
Cuts at: 838 838
Size: 3839
PpiI GAACnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 788 820 2978 3010 788
Size: 32 2158 32 1617
Fragments arranged by size:
2158 1617 32 32
PpuMI rG'GwC_Cy
Cuts at: 543 543
Size: 3839
PspXI vC'TCGA_Gb
Cuts at: 744 744
Size: 3839
PstI C_TGCA'G
Cuts at: 176 314 542 658 886 1571 176
Size: 138 228 116 228 685 2444
Fragments arranged by size:
2444 685 228 228 138 116
PvuI CG_AT'CG
Cuts at: 104 104
Size: 3839
PvuII CAG'CTG
Cuts at: 1264 1624 1264
Size: 360 3479
RsrII CG'GwC_CG
Cuts at: 2034 2034
Size: 3839
SacI G_AGCT'C
Cuts at: 853 853
Size: 3839
SalI G'TCGA_C
Cuts at: 876 876
Size: 3839
SapI GCTCTTCn'nnn_
Cuts at: 1862 2072 1862
Size: 210 3629
SbfI CC_TGCA'GG
Cuts at: 886 886
Size: 3839
ScaI AGT'ACT
Cuts at: 3356 3356
Size: 3839
SfcI C'TryA_G
Cuts at: 33 172 310 538 606 654 882 1567 2560
Size: 139 138 228 68 48 228 685 993
Cuts at: 2560 2751 33
Size: 191 1121
Fragments arranged by size:
1121 993 685 228 228 191 139 138
68 48
SfoI GGC'GCC
Cuts at: 299 1271 1519 299
Size: 972 248 2619
Fragments arranged by size:
2619 972 248
SgfI GCG_AT'CGC
Cuts at: 104 104
Size: 3839
SmaI CCC'GGG
Cuts at: 305 861 305
Size: 556 3283
SmlI C'TyrA_G
Cuts at: 628 744 994 1909 2401 2663 628
Size: 116 250 915 492 262 1804
Fragments arranged by size:
1804 915 492 262 250 116
SpeI A'CTAG_T
Cuts at: 67 67
Size: 3839
SphI G_CATG'C
Cuts at: 892 1392 892
Size: 500 3339
StuI AGG'CCT
Cuts at: 442 442
Size: 3839
StyI C'CwwG_G
Cuts at: 494 973 1327 494
Size: 479 354 3006
Fragments arranged by size:
3006 479 354
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 1422 1555 2014 2223 1422
Size: 133 459 209 3038
Fragments arranged by size:
3038 459 209 133
TatI w'GTAC_w
Cuts at: 3354 3354
Size: 3839
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 3356 3388 3356
Size: 32 3807
Tth111I GACn'n_nGTC
Cuts at: 1636 1636
Size: 3839
XbaI T'CTAG_A
Cuts at: 870 870
Size: 3839
XcmI CCAnnnn_n'nnnnTGG
Cuts at: 1334 1334
Size: 3839
XhoI C'TCGA_G
Cuts at: 744 744
Size: 3839
XmaI C'CCGG_G
Cuts at: 303 859 303
Size: 556 3283
Enzymes that do cut:
AarI AccI Acc65I AclI AcuI AflIII AgeI AloI
AlwNI ApaLI ApoI AseI AsiSI AvaI BamHI BanI
BanII BbeI BciVI BclI BglI BglII BlpI Bme1580I
BmrI BplI Bpu10I BpuEI BsaI BsaAI BsaBI BsaHI
BsaWI BsaXI BseRI BseYI BsgI BsiEI BsiHKAI Bsp1286I
BspEI BspHI BspMI BsrBI BsrDI BsrFI BssSI BstAPI
BstBI BstXI BstYI BtgZI BtsI DraI DrdI EaeI
EagI EarI EciI EcoICRI Eco57MI EcoO109I EcoRI FalI
FspI HaeII Hin4I HincII KasI KpnI MluI MmeI
MscI MslI MspA1I NaeI NarI NgoMIV NspI PciI
PmeI PpiI PpuMI PspXI PstI PvuI PvuII RsrII
SacI SalI SapI SbfI ScaI SfcI SfoI SgfI
SmaI SmlI SpeI SphI StuI StyI TaqII TatI
TstI Tth111I XbaI XcmI XhoI XmaI
Enzymes that do not cut:
AatII AfeI AflII AhdI AleI AlfI ApaI AscI
AvrII BaeI BbsI BbvCI BcgI BfrBI BmgBI BmtI
BpmI BsiWI BsmI BsmBI BsrGI BssHII BstEII BstZ17I
Bsu36I BtgI ClaI CspCI DraIII EcoNI EcoRV FseI
FspAI HindIII HpaI MfeI NcoI NdeI NheI NotI
NruI NsiI PacI PasI PflMI PfoI PmlI PshAI
PsiI PspOMI PsrI SacII SanDI SexAI SfiI SgrAI
SnaBI SrfI SspI SwaI XmnI ZraI