Restriction map for
pF1KB7452
(Circular) (Six-base) MAPSORT of: pF1KB7452.seq Check: 6844 from: 1 to: 3926
pF1KB7452 3926 bp
With 182 enzymes: SgfI *
June 20, 2008 10:27 ..
AarI CACCTGCnnnn'nnnn_
Cuts at: 676 676
Size: 3926
AccI GT'mk_AC
Cuts at: 964 964
Size: 3926
Acc65I G'GTAC_C
Cuts at: 138 942 138
Size: 804 3122
AclI AA'CG_TT
Cuts at: 3766 3766
Size: 3926
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 286 1751 2183 2930 3198 286
Size: 1465 432 747 268 1014
Fragments arranged by size:
1465 1014 747 432 268
AflIII A'CryG_T
Cuts at: 1278 2382 3359 1278
Size: 1104 977 1845
Fragments arranged by size:
1845 1104 977
AgeI A'CCGG_T
Cuts at: 2314 2314
Size: 3926
AlwNI CAG_nnn'CTG
Cuts at: 381 399 720 1231 2798 381
Size: 18 321 511 1567 1509
Fragments arranged by size:
1567 1509 511 321 18
ApaLI G'TGCA_C
Cuts at: 2696 2696
Size: 3926
ApoI r'AATT_y
Cuts at: 930 3279 3535 930
Size: 2349 256 1321
Fragments arranged by size:
2349 1321 256
AseI AT'TA_AT
Cuts at: 20 20
Size: 3926
AsiSI GCG_AT'CGC
Cuts at: 104 104
Size: 3926
AvaI C'yCGr_G
Cuts at: 185 286 369 577 946 185
Size: 101 83 208 369 3165
Fragments arranged by size:
3165 369 208 101 83
BamHI G'GATC_C
Cuts at: 951 951
Size: 3926
BanI G'GyrC_C
Cuts at: 138 355 416 591 633 849 942 1356 1604
Size: 217 61 175 42 216 93 414 248
Cuts at: 1604 1639 138
Size: 35 2425
Fragments arranged by size:
2425 414 248 217 216 175 93 61
42 35
BanII G_rGCy'C
Cuts at: 368 940 1970 368
Size: 572 1030 2324
Fragments arranged by size:
2324 1030 572
BbeI G_GCGC'C
Cuts at: 359 595 853 1360 1608 359
Size: 236 258 507 248 2677
Fragments arranged by size:
2677 507 258 248 236
BbsI GAAGACnn'nnnn_
Cuts at: 639 639
Size: 3926
BciVI GTATCCnnnnn_n'
Cuts at: 192 1824 2585 3926 192
Size: 1632 761 1341 192
Fragments arranged by size:
1632 1341 761 192
BclI T'GATC_A
Cuts at: 1446 1446
Size: 3926
BglI GCCn_nnn'nGGC
Cuts at: 112 307 566 742 758 112
Size: 195 259 176 16 3280
Fragments arranged by size:
3280 259 195 176 16
BglII A'GATC_T
Cuts at: 1441 3478 1441
Size: 2037 1889
BlpI GC'TnA_GC
Cuts at: 640 1038 640
Size: 398 3528
Bme1580I G_kGCm'C
Cuts at: 661 1551 1644 2700 661
Size: 890 93 1056 1887
Fragments arranged by size:
1887 1056 890 93
BmrI ACTGGGnnnn_n'
Cuts at: 1138 1219 1554 3698 1138
Size: 81 335 2144 1366
Fragments arranged by size:
2144 1366 335 81
BplI GAGnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 855 2293 855
Size: 1438 2488
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 295 703 742 802 850 895 295
Size: 408 39 60 48 45 3326
Fragments arranged by size:
3326 408 60 48 45 39
Bpu10I CC'TnA_GC
Cuts at: 487 547 487
Size: 60 3866
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 1102 1981 2473 2771 1102
Size: 879 492 298 2257
Fragments arranged by size:
2257 879 492 298
BsaI GGTCTCn'nnnn_
Cuts at: 3630 3630
Size: 3926
BsaAI yAC'GTr
Cuts at: 253 1279 253
Size: 1026 2900
BsaBI GATnn'nnATC
Cuts at: 6 1440 3477 6
Size: 1434 2037 455
Fragments arranged by size:
2037 1434 455
BsaHI Gr'CG_yC
Cuts at: 356 592 806 850 1357 1605 2307 3803 356
Size: 236 214 44 507 248 702 1496 479
Fragments arranged by size:
1496 702 507 479 248 236 214 44
BsaWI w'CCGG_w
Cuts at: 1115 1636 2314 2588 2735 3146 1115
Size: 521 678 274 147 411 1895
Fragments arranged by size:
1895 678 521 411 274 147
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 226 304 595 880 226
Size: 78 291 285 3272
Fragments arranged by size:
3272 291 285 78
BseYI C'CCAG_C
Cuts at: 1351 2109 2686 3182 1351
Size: 758 577 496 2095
Fragments arranged by size:
2095 758 577 496
BsgI GTGCAGnnnnnnnnnnnnnn_nn'
Cuts at: 631 631
Size: 3926
BsiEI CG_ry'CG
Cuts at: 104 152 171 203 299 734 750 1468 1514
Size: 48 19 32 96 435 16 718 46
Cuts at: 1514 2722 104
Size: 1208 1308
Fragments arranged by size:
1308 1208 718 435 96 48 46 32
19 16
BsiHKAI G_wGCw'C
Cuts at: 940 1718 1908 2700 940
Size: 778 190 792 2166
Fragments arranged by size:
2166 792 778 190
Bsp1286I G_dGCh'C
Cuts at: 368 661 940 1551 1644 1718 1908 1970 2700
Size: 293 279 611 93 74 190 62 730
Cuts at: 2700 368
Size: 1594
Fragments arranged by size:
1594 730 611 293 279 190 93 74
62
BspEI T'CCGG_A
Cuts at: 3146 3146
Size: 3926
BspHI T'CATG_A
Cuts at: 3102 3102
Size: 3926
BspMI ACCTGCnnnn'nnnn_
Cuts at: 219 676 976 1190 1492 1873 219
Size: 457 300 214 302 381 2272
Fragments arranged by size:
2272 457 381 302 300 214
BsrBI CCG'CTC
Cuts at: 50 2218 2272 3756 3921 50
Size: 2168 54 1484 165 55
Fragments arranged by size:
2168 1484 165 55 54
BsrDI GCAATG_nn'
Cuts at: 1838 1838
Size: 3926
BsrFI r'CCGG_y
Cuts at: 147 151 493 700 835 912 1924 2105 2314
Size: 4 342 207 135 77 1012 181 209
Cuts at: 2314 147
Size: 1759
Fragments arranged by size:
1759 1012 342 209 207 181 135 77
4
BssHII G'CGCG_C
Cuts at: 407 694 696 704 788 820 407
Size: 287 2 8 84 32 3513
Fragments arranged by size:
3513 287 84 32 8 2
BssSI C'ACGA_G
Cuts at: 625 2197 2555 625
Size: 1572 358 1996
Fragments arranged by size:
1996 1572 358
BstAPI GCAn_nnn'nTGC
Cuts at: 1303 1303
Size: 3926
BstBI TT'CG_AA
Cuts at: 2287 2287
Size: 3926
BstYI r'GATC_y
Cuts at: 1 951 1418 1441 1776 2022 3034 3120 3132
Size: 950 467 23 335 246 1012 86 12
Cuts at: 3132 3478 1
Size: 346 449
Fragments arranged by size:
1012 950 467 449 346 335 246 86
23 12
BtgI C'CryG_G
Cuts at: 107 740 107
Size: 633 3293
BtgZI GCGATGnnnnnnnnnn'nnnn_
Cuts at: 882 1876 2057 2220 882
Size: 994 181 163 2588
Fragments arranged by size:
2588 994 181 163
BtsI GCAGTG_nn'
Cuts at: 122 619 1169 122
Size: 497 550 2879
Fragments arranged by size:
2879 550 497
DraI TTT'AAA
Cuts at: 925 925
Size: 3926
DrdI GACnn_nn'nnGTC
Cuts at: 1632 2490 1632
Size: 858 3068
EaeI y'GGCC_r
Cuts at: 149 168 200 567 731 743 1511 1685 2076
Size: 19 32 367 164 12 768 174 391
Cuts at: 2076 2103 3448 149
Size: 27 1345 627
Fragments arranged by size:
1345 768 627 391 367 174 164 32
27 19 12
EagI C'GGCC_G
Cuts at: 149 168 200 731 1511 149
Size: 19 32 531 780 2564
Fragments arranged by size:
2564 780 531 32 19
EarI CTCTTCn'nnn_
Cuts at: 502 715 898 1949 2159 502
Size: 213 183 1051 210 2269
Fragments arranged by size:
2269 1051 213 210 183
EciI GGCGGAnnnnnnnnn_nn'
Cuts at: 2134 2444 2590 3736 2134
Size: 310 146 1146 2324
Fragments arranged by size:
2324 1146 310 146
EcoICRI GAG'CTC
Cuts at: 938 938
Size: 3926
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 286 295 703 742 802 850 895 1751 2183
Size: 9 408 39 60 48 45 856 432
Cuts at: 2183 2930 3198 286
Size: 747 268 1014
Fragments arranged by size:
1014 856 747 432 408 268 60 48
45 39 9
EcoNI CCTnn'n_nnAGG
Cuts at: 226 265 226
Size: 39 3887
EcoO109I rG'GnC_Cy
Cuts at: 1065 1065
Size: 3926
EcoRI G'AATT_C
Cuts at: 930 930
Size: 3926
FalI AAGnnnnnCTTnnnnnnnn_nnnnn'
Cuts at: 1945 1945
Size: 3926
FspI TGC'GCA
Cuts at: 604 1707 604
Size: 1103 2823
HaeII r_GCGC'y
Cuts at: 359 399 494 595 853 1360 1608 2630 3609
Size: 40 95 101 258 507 248 1022 979
Cuts at: 3609 359
Size: 676
Fragments arranged by size:
1022 979 676 507 258 248 101 95
40
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 1768 1798 1800 1830 1768
Size: 30 2 30 3864
Fragments arranged by size:
3864 30 30 2
HincII GTy'rAC
Cuts at: 965 965
Size: 3926
KasI G'GCGC_C
Cuts at: 355 591 849 1356 1604 355
Size: 236 258 507 248 2677
Fragments arranged by size:
2677 507 258 248 236
KpnI G_GTAC'C
Cuts at: 142 946 142
Size: 804 3122
MluI A'CGCG_T
Cuts at: 3359 3359
Size: 3926
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 2597 2781 2597
Size: 184 3742
MscI TGG'CCA
Cuts at: 1687 1687
Size: 3926
MslI CAynn'nnrTG
Cuts at: 1915 2042 1915
Size: 127 3799
MspA1I CmG'CkG
Cuts at: 167 597 639 717 1037 1351 1711 2724 2969
Size: 430 42 78 320 314 360 1013 245
Cuts at: 2969 167
Size: 1124
Fragments arranged by size:
1124 1013 430 360 320 314 245 78
42
NaeI GCC'GGC
Cuts at: 149 495 702 837 2107 149
Size: 346 207 135 1270 1968
Fragments arranged by size:
1968 1270 346 207 135
NarI GG'CG_CC
Cuts at: 356 592 850 1357 1605 356
Size: 236 258 507 248 2677
Fragments arranged by size:
2677 507 258 248 236
NcoI C'CATG_G
Cuts at: 107 107
Size: 3926
NgoMIV G'CCGG_C
Cuts at: 147 493 700 835 2105 147
Size: 346 207 135 1270 1968
Fragments arranged by size:
1968 1270 346 207 135
NotI GC'GGCC_GC
Cuts at: 168 168
Size: 3926
NruI TCG'CGA
Cuts at: 901 901
Size: 3926
NspI r_CATG'y
Cuts at: 979 1479 2386 979
Size: 500 907 2519
Fragments arranged by size:
2519 907 500
PciI A'CATG_T
Cuts at: 2382 2382
Size: 3926
PflMI CCAn_nnn'nTGG
Cuts at: 720 720
Size: 3926
PfoI T'CCnGG_A
Cuts at: 827 827
Size: 3926
PmeI GTTT'AAAC
Cuts at: 925 925
Size: 3926
PpiI GAACnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 3065 3097 3065
Size: 32 3894
PstI C_TGCA'G
Cuts at: 389 797 973 1658 389
Size: 408 176 685 2657
Fragments arranged by size:
2657 685 408 176
PvuI CG_AT'CG
Cuts at: 104 299 104
Size: 195 3731
PvuII CAG'CTG
Cuts at: 717 1351 1711 717
Size: 634 360 2932
Fragments arranged by size:
2932 634 360
RsrII CG'GwC_CG
Cuts at: 2121 2121
Size: 3926
SacI G_AGCT'C
Cuts at: 940 940
Size: 3926
SalI G'TCGA_C
Cuts at: 963 963
Size: 3926
SapI GCTCTTCn'nnn_
Cuts at: 715 898 1949 2159 715
Size: 183 1051 210 2482
Fragments arranged by size:
2482 1051 210 183
SbfI CC_TGCA'GG
Cuts at: 973 973
Size: 3926
ScaI AGT'ACT
Cuts at: 3443 3443
Size: 3926
SfcI C'TryA_G
Cuts at: 33 385 534 793 969 1654 2647 2838 33
Size: 352 149 259 176 685 993 191 1121
Fragments arranged by size:
1121 993 685 352 259 191 176 149
SfoI GGC'GCC
Cuts at: 357 593 851 1358 1606 357
Size: 236 258 507 248 2677
Fragments arranged by size:
2677 507 258 248 236
SgfI GCG_AT'CGC
Cuts at: 104 104
Size: 3926
SgrAI Cr'CCGG_yG
Cuts at: 493 700 493
Size: 207 3719
SmaI CCC'GGG
Cuts at: 187 371 948 187
Size: 184 577 3165
Fragments arranged by size:
3165 577 184
SmlI C'TyrA_G
Cuts at: 1081 1996 2488 2750 1081
Size: 915 492 262 2257
Fragments arranged by size:
2257 915 492 262
SpeI A'CTAG_T
Cuts at: 67 67
Size: 3926
SphI G_CATG'C
Cuts at: 979 1479 979
Size: 500 3426
StyI C'CwwG_G
Cuts at: 107 1060 1414 107
Size: 953 354 2619
Fragments arranged by size:
2619 953 354
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 1509 1642 2101 2310 1509
Size: 133 459 209 3125
Fragments arranged by size:
3125 459 209 133
TatI w'GTAC_w
Cuts at: 3441 3441
Size: 3926
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 3443 3475 3443
Size: 32 3894
Tth111I GACn'n_nGTC
Cuts at: 1723 1723
Size: 3926
XbaI T'CTAG_A
Cuts at: 957 957
Size: 3926
XcmI CCAnnnn_n'nnnnTGG
Cuts at: 114 1421 114
Size: 1307 2619
XmaI C'CCGG_G
Cuts at: 185 369 946 185
Size: 184 577 3165
Fragments arranged by size:
3165 577 184
Enzymes that do cut:
AarI AccI Acc65I AclI AcuI AflIII AgeI AlwNI
ApaLI ApoI AseI AsiSI AvaI BamHI BanI BanII
BbeI BbsI BciVI BclI BglI BglII BlpI Bme1580I
BmrI BplI BpmI Bpu10I BpuEI BsaI BsaAI BsaBI
BsaHI BsaWI BseRI BseYI BsgI BsiEI BsiHKAI Bsp1286I
BspEI BspHI BspMI BsrBI BsrDI BsrFI BssHII BssSI
BstAPI BstBI BstYI BtgI BtgZI BtsI DraI DrdI
EaeI EagI EarI EciI EcoICRI Eco57MI EcoNI EcoO109I
EcoRI FalI FspI HaeII Hin4I HincII KasI KpnI
MluI MmeI MscI MslI MspA1I NaeI NarI NcoI
NgoMIV NotI NruI NspI PciI PflMI PfoI PmeI
PpiI PstI PvuI PvuII RsrII SacI SalI SapI
SbfI ScaI SfcI SfoI SgfI SgrAI SmaI SmlI
SpeI SphI StyI TaqII TatI TstI Tth111I XbaI
XcmI XmaI
Enzymes that do not cut:
AatII AfeI AflII AhdI AleI AlfI AloI ApaI
AscI AvrII BaeI BbvCI BcgI BfrBI BmgBI BmtI
BsaXI BsiWI BsmI BsmBI BsrGI BstEII BstXI BstZ17I
Bsu36I ClaI CspCI DraIII EcoRV FseI FspAI HindIII
HpaI MfeI NdeI NheI NsiI PacI PasI PmlI
PpuMI PshAI PsiI PspOMI PspXI PsrI SacII SanDI
SexAI SfiI SnaBI SrfI SspI StuI SwaI XhoI
XmnI ZraI