Restriction map for
pF1KB7429
(Circular) (Six-base) MAPSORT of: pF1KB7429.seq Check: 6927 from: 1 to: 4010
pF1KB7429 4010 bp
With 182 enzymes: SgfI *
June 20, 2008 10:27 ..
AarI CACCTGCnnnn'nnnn_
Cuts at: 533 533
Size: 4010
AccI GT'mk_AC
Cuts at: 1048 1048
Size: 4010
Acc65I G'GTAC_C
Cuts at: 1026 1026
Size: 4010
AclI AA'CG_TT
Cuts at: 3850 3850
Size: 4010
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 1835 2267 3014 3282 1835
Size: 432 747 268 2563
Fragments arranged by size:
2563 747 432 268
AfeI AGC'GCT
Cuts at: 880 880
Size: 4010
AflIII A'CryG_T
Cuts at: 1362 2466 3443 1362
Size: 1104 977 1929
Fragments arranged by size:
1929 1104 977
AgeI A'CCGG_T
Cuts at: 2398 2398
Size: 4010
AleI CACnn'nnGTG
Cuts at: 649 649
Size: 4010
AlfI GCAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 946 946
Size: 4010
AlwNI CAG_nnn'CTG
Cuts at: 304 1315 2882 304
Size: 1011 1567 1432
Fragments arranged by size:
1567 1432 1011
ApaI G_GGCC'C
Cuts at: 703 733 703
Size: 30 3980
ApaLI G'TGCA_C
Cuts at: 2780 2780
Size: 4010
ApoI r'AATT_y
Cuts at: 1014 3363 3619 1014
Size: 2349 256 1405
Fragments arranged by size:
2349 1405 256
AseI AT'TA_AT
Cuts at: 20 20
Size: 4010
AsiSI GCG_AT'CGC
Cuts at: 104 104
Size: 4010
AvaI C'yCGr_G
Cuts at: 1030 1030
Size: 4010
BamHI G'GATC_C
Cuts at: 1035 1035
Size: 4010
BanI G'GyrC_C
Cuts at: 141 521 870 902 982 1026 1440 1688 1723
Size: 380 349 32 80 44 414 248 35
Cuts at: 1723 141
Size: 2428
Fragments arranged by size:
2428 414 380 349 248 80 44 35
32
BanII G_rGCy'C
Cuts at: 703 733 1024 2054 703
Size: 30 291 1030 2659
Fragments arranged by size:
2659 1030 291 30
BbeI G_GCGC'C
Cuts at: 145 874 906 1444 1692 145
Size: 729 32 538 248 2463
Fragments arranged by size:
2463 729 538 248 32
BciVI GTATCCnnnnn_n'
Cuts at: 967 1908 2669 4010 967
Size: 941 761 1341 967
Fragments arranged by size:
1341 967 941 761
BclI T'GATC_A
Cuts at: 1530 1530
Size: 4010
BglII A'GATC_T
Cuts at: 1525 3562 1525
Size: 2037 1973
BlpI GC'TnA_GC
Cuts at: 214 1122 214
Size: 908 3102
Bme1580I G_kGCm'C
Cuts at: 519 703 733 935 985 1635 1728 2784 519
Size: 184 30 202 50 650 93 1056 1745
Fragments arranged by size:
1745 1056 650 202 184 93 50 30
BmgBI CAC'GTC
Cuts at: 852 942 852
Size: 90 3920
BmrI ACTGGGnnnn_n'
Cuts at: 597 706 1222 1303 1638 3782 597
Size: 109 516 81 335 2144 825
Fragments arranged by size:
2144 825 516 335 109 81
BplI GAGnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 234 2377 234
Size: 2143 1867
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 358 373 358
Size: 15 3995
Bpu10I CC'TnA_GC
Cuts at: 631 631
Size: 4010
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 742 1186 2065 2557 2855 742
Size: 444 879 492 298 1897
Fragments arranged by size:
1897 879 492 444 298
BsaI GGTCTCn'nnnn_
Cuts at: 808 3714 808
Size: 2906 1104
BsaAI yAC'GTr
Cuts at: 1363 1363
Size: 4010
BsaBI GATnn'nnATC
Cuts at: 6 619 1524 3561 6
Size: 613 905 2037 455
Fragments arranged by size:
2037 905 613 455
BsaHI Gr'CG_yC
Cuts at: 142 871 903 1441 1689 2391 3887 142
Size: 729 32 538 248 702 1496 265
Fragments arranged by size:
1496 729 702 538 265 248 32
BsaWI w'CCGG_w
Cuts at: 1199 1720 2398 2672 2819 3230 1199
Size: 521 678 274 147 411 1979
Fragments arranged by size:
1979 678 521 411 274 147
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 261 261
Size: 4010
BseYI C'CCAG_C
Cuts at: 803 1435 2193 2770 3266 803
Size: 632 758 577 496 1547
Fragments arranged by size:
1547 758 632 577 496
BsgI GTGCAGnnnnnnnnnnnnnn_nn'
Cuts at: 673 720 673
Size: 47 3963
BsiEI CG_ry'CG
Cuts at: 104 1552 1598 2806 104
Size: 1448 46 1208 1308
Fragments arranged by size:
1448 1308 1208 46
BsiHKAI G_wGCw'C
Cuts at: 319 1024 1802 1992 2784 319
Size: 705 778 190 792 1545
Fragments arranged by size:
1545 792 778 705 190
Bsp1286I G_dGCh'C
Cuts at: 319 519 703 733 935 985 1024 1635 1728
Size: 200 184 30 202 50 39 611 93
Cuts at: 1728 1802 1992 2054 2784 319
Size: 74 190 62 730 1545
Fragments arranged by size:
1545 730 611 202 200 190 184 93
74 62 50 39 30
BspEI T'CCGG_A
Cuts at: 3230 3230
Size: 4010
BspHI T'CATG_A
Cuts at: 3186 3186
Size: 4010
BspMI ACCTGCnnnn'nnnn_
Cuts at: 533 587 647 914 1060 1274 1576 1957 533
Size: 54 60 267 146 214 302 381 2586
Fragments arranged by size:
2586 381 302 267 214 146 60 54
BsrBI CCG'CTC
Cuts at: 50 153 208 342 442 2302 2356 3840 4005
Size: 103 55 134 100 1860 54 1484 165
Cuts at: 4005 50
Size: 55
Fragments arranged by size:
1860 1484 165 134 103 100 55 55
54
BsrDI GCAATG_nn'
Cuts at: 1922 1922
Size: 4010
BsrFI r'CCGG_y
Cuts at: 121 817 919 2008 2189 2398 121
Size: 696 102 1089 181 209 1733
Fragments arranged by size:
1733 1089 696 209 181 102
BssHII G'CGCG_C
Cuts at: 743 743
Size: 4010
BssSI C'ACGA_G
Cuts at: 382 778 2281 2639 382
Size: 396 1503 358 1753
Fragments arranged by size:
1753 1503 396 358
BstAPI GCAn_nnn'nTGC
Cuts at: 989 1387 989
Size: 398 3612
BstBI TT'CG_AA
Cuts at: 2371 2371
Size: 4010
BstYI r'GATC_y
Cuts at: 1 1035 1502 1525 1860 2106 3118 3204 3216
Size: 1034 467 23 335 246 1012 86 12
Cuts at: 3216 3562 1
Size: 346 449
Fragments arranged by size:
1034 1012 467 449 346 335 246 86
23 12
Bsu36I CC'TnA_GG
Cuts at: 163 798 163
Size: 635 3375
BtgI C'CryG_G
Cuts at: 320 410 320
Size: 90 3920
BtgZI GCGATGnnnnnnnnnn'nnnn_
Cuts at: 1960 2141 2304 1960
Size: 181 163 3666
Fragments arranged by size:
3666 181 163
BtsI GCAGTG_nn'
Cuts at: 506 1253 506
Size: 747 3263
CspCI CAAnnnnnGTGGnnnnnnnnnn_nn'
Cuts at: 219 254 219
Size: 35 3975
DraI TTT'AAA
Cuts at: 1009 1009
Size: 4010
DraIII CAC_nnn'GTG
Cuts at: 605 915 605
Size: 310 3700
DrdI GACnn_nn'nnGTC
Cuts at: 1716 2574 1716
Size: 858 3152
EaeI y'GGCC_r
Cuts at: 749 888 917 1595 1769 2160 2187 3532 749
Size: 139 29 678 174 391 27 1345 1227
Fragments arranged by size:
1345 1227 678 391 174 139 29 27
EagI C'GGCC_G
Cuts at: 1595 1595
Size: 4010
EarI CTCTTCn'nnn_
Cuts at: 143 198 2033 2243 143
Size: 55 1835 210 1910
Fragments arranged by size:
1910 1835 210 55
EciI GGCGGAnnnnnnnnn_nn'
Cuts at: 2218 2528 2674 3820 2218
Size: 310 146 1146 2408
Fragments arranged by size:
2408 1146 310 146
EcoICRI GAG'CTC
Cuts at: 1022 1022
Size: 4010
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 358 373 1835 2267 3014 3282 358
Size: 15 1462 432 747 268 1086
Fragments arranged by size:
1462 1086 747 432 268 15
EcoNI CCTnn'n_nnAGG
Cuts at: 264 264
Size: 4010
EcoO109I rG'GnC_Cy
Cuts at: 167 301 591 700 729 730 1149 167
Size: 134 290 109 29 1 419 3028
Fragments arranged by size:
3028 419 290 134 109 29 1
EcoRI G'AATT_C
Cuts at: 1014 1014
Size: 4010
FalI AAGnnnnnCTTnnnnnnnn_nnnnn'
Cuts at: 2029 2029
Size: 4010
FspI TGC'GCA
Cuts at: 1791 1791
Size: 4010
HaeII r_GCGC'y
Cuts at: 145 874 882 906 1444 1692 2714 3693 145
Size: 729 8 24 538 248 1022 979 462
Fragments arranged by size:
1022 979 729 538 462 248 24 8
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 781 813 1852 1882 1884 1914 781
Size: 32 1039 30 2 30 2877
Fragments arranged by size:
2877 1039 32 30 30 2
HincII GTy'rAC
Cuts at: 1049 1049
Size: 4010
KasI G'GCGC_C
Cuts at: 141 870 902 1440 1688 141
Size: 729 32 538 248 2463
Fragments arranged by size:
2463 729 538 248 32
KpnI G_GTAC'C
Cuts at: 1030 1030
Size: 4010
MluI A'CGCG_T
Cuts at: 3443 3443
Size: 4010
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 116 2681 2865 116
Size: 2565 184 1261
Fragments arranged by size:
2565 1261 184
MscI TGG'CCA
Cuts at: 751 1771 751
Size: 1020 2990
MslI CAynn'nnrTG
Cuts at: 404 649 1999 2126 404
Size: 245 1350 127 2288
Fragments arranged by size:
2288 1350 245 127
MspA1I CmG'CkG
Cuts at: 322 1121 1435 1795 2808 3053 322
Size: 799 314 360 1013 245 1279
Fragments arranged by size:
1279 1013 799 360 314 245
NaeI GCC'GGC
Cuts at: 123 921 2191 123
Size: 798 1270 1942
Fragments arranged by size:
1942 1270 798
NarI GG'CG_CC
Cuts at: 142 871 903 1441 1689 142
Size: 729 32 538 248 2463
Fragments arranged by size:
2463 729 538 248 32
NgoMIV G'CCGG_C
Cuts at: 121 919 2189 121
Size: 798 1270 1942
Fragments arranged by size:
1942 1270 798
NspI r_CATG'y
Cuts at: 1063 1563 2470 1063
Size: 500 907 2603
Fragments arranged by size:
2603 907 500
PciI A'CATG_T
Cuts at: 2466 2466
Size: 4010
PflMI CCAn_nnn'nTGG
Cuts at: 915 915
Size: 4010
PfoI T'CCnGG_A
Cuts at: 448 448
Size: 4010
PmeI GTTT'AAAC
Cuts at: 1009 1009
Size: 4010
PpiI GAACnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 3149 3181 3149
Size: 32 3978
PpuMI rG'GwC_Cy
Cuts at: 591 591
Size: 4010
PspOMI G'GGCC_C
Cuts at: 699 729 699
Size: 30 3980
PstI C_TGCA'G
Cuts at: 182 1057 1742 182
Size: 875 685 2450
Fragments arranged by size:
2450 875 685
PvuI CG_AT'CG
Cuts at: 104 104
Size: 4010
PvuII CAG'CTG
Cuts at: 1435 1795 1435
Size: 360 3650
RsrII CG'GwC_CG
Cuts at: 2205 2205
Size: 4010
SacI G_AGCT'C
Cuts at: 1024 1024
Size: 4010
SacII CC_GC'GG
Cuts at: 323 323
Size: 4010
SalI G'TCGA_C
Cuts at: 1047 1047
Size: 4010
SapI GCTCTTCn'nnn_
Cuts at: 143 198 2033 2243 143
Size: 55 1835 210 1910
Fragments arranged by size:
1910 1835 210 55
SbfI CC_TGCA'GG
Cuts at: 1057 1057
Size: 4010
ScaI AGT'ACT
Cuts at: 3527 3527
Size: 4010
SfcI C'TryA_G
Cuts at: 33 178 1053 1738 2731 2922 33
Size: 145 875 685 993 191 1121
Fragments arranged by size:
1121 993 875 685 191 145
SfoI GGC'GCC
Cuts at: 143 872 904 1442 1690 143
Size: 729 32 538 248 2463
Fragments arranged by size:
2463 729 538 248 32
SgfI GCG_AT'CGC
Cuts at: 104 104
Size: 4010
SmaI CCC'GGG
Cuts at: 1032 1032
Size: 4010
SmlI C'TyrA_G
Cuts at: 757 1165 2080 2572 2834 757
Size: 408 915 492 262 1933
Fragments arranged by size:
1933 915 492 408 262
SpeI A'CTAG_T
Cuts at: 67 67
Size: 4010
SphI G_CATG'C
Cuts at: 1063 1563 1063
Size: 500 3510
StuI AGG'CCT
Cuts at: 259 259
Size: 4010
StyI C'CwwG_G
Cuts at: 170 422 1144 1498 170
Size: 252 722 354 2682
Fragments arranged by size:
2682 722 354 252
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 616 1593 1726 2185 2394 616
Size: 977 133 459 209 2232
Fragments arranged by size:
2232 977 459 209 133
TatI w'GTAC_w
Cuts at: 3525 3525
Size: 4010
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 3527 3559 3527
Size: 32 3978
Tth111I GACn'n_nGTC
Cuts at: 991 1807 991
Size: 816 3194
XbaI T'CTAG_A
Cuts at: 1041 1041
Size: 4010
XcmI CCAnnnn_n'nnnnTGG
Cuts at: 570 1505 570
Size: 935 3075
XmaI C'CCGG_G
Cuts at: 1030 1030
Size: 4010
Enzymes that do cut:
AarI AccI Acc65I AclI AcuI AfeI AflIII AgeI
AleI AlfI AlwNI ApaI ApaLI ApoI AseI AsiSI
AvaI BamHI BanI BanII BbeI BciVI BclI BglII
BlpI Bme1580I BmgBI BmrI BplI BpmI Bpu10I BpuEI
BsaI BsaAI BsaBI BsaHI BsaWI BseRI BseYI BsgI
BsiEI BsiHKAI Bsp1286I BspEI BspHI BspMI BsrBI BsrDI
BsrFI BssHII BssSI BstAPI BstBI BstYI Bsu36I BtgI
BtgZI BtsI CspCI DraI DraIII DrdI EaeI EagI
EarI EciI EcoICRI Eco57MI EcoNI EcoO109I EcoRI FalI
FspI HaeII Hin4I HincII KasI KpnI MluI MmeI
MscI MslI MspA1I NaeI NarI NgoMIV NspI PciI
PflMI PfoI PmeI PpiI PpuMI PspOMI PstI PvuI
PvuII RsrII SacI SacII SalI SapI SbfI ScaI
SfcI SfoI SgfI SmaI SmlI SpeI SphI StuI
StyI TaqII TatI TstI Tth111I XbaI XcmI XmaI
Enzymes that do not cut:
AatII AflII AhdI AloI AscI AvrII BaeI BbsI
BbvCI BcgI BfrBI BglI BmtI BsaXI BsiWI BsmI
BsmBI BsrGI BstEII BstXI BstZ17I ClaI EcoRV FseI
FspAI HindIII HpaI MfeI NcoI NdeI NheI NotI
NruI NsiI PacI PasI PmlI PshAI PsiI PspXI
PsrI SanDI SexAI SfiI SgrAI SnaBI SrfI SspI
SwaI XhoI XmnI ZraI