Restriction map for
pF1KB7408
(Circular) (Six-base) MAPSORT of: pF1KB7408.seq Check: 7805 from: 1 to: 4295
pF1KB7408 4295 bp
With 182 enzymes: SgfI *
June 20, 2008 10:26 ..
AccI GT'mk_AC
Cuts at: 1333 1333
Size: 4295
Acc65I G'GTAC_C
Cuts at: 503 1311 503
Size: 808 3487
AclI AA'CG_TT
Cuts at: 4135 4135
Size: 4295
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 2120 2552 3299 3567 2120
Size: 432 747 268 2848
Fragments arranged by size:
2848 747 432 268
AflIII A'CryG_T
Cuts at: 1647 2751 3728 1647
Size: 1104 977 2214
Fragments arranged by size:
2214 1104 977
AgeI A'CCGG_T
Cuts at: 2683 2683
Size: 4295
AleI CACnn'nnGTG
Cuts at: 512 601 512
Size: 89 4206
AlwNI CAG_nnn'CTG
Cuts at: 1600 3167 1600
Size: 1567 2728
ApaLI G'TGCA_C
Cuts at: 3065 3065
Size: 4295
ApoI r'AATT_y
Cuts at: 660 1003 1026 1299 3648 3904 660
Size: 343 23 273 2349 256 1051
Fragments arranged by size:
2349 1051 343 273 256 23
AseI AT'TA_AT
Cuts at: 20 895 20
Size: 875 3420
AsiSI GCG_AT'CGC
Cuts at: 104 104
Size: 4295
AvaI C'yCGr_G
Cuts at: 1315 1315
Size: 4295
BamHI G'GATC_C
Cuts at: 1320 1320
Size: 4295
BanI G'GyrC_C
Cuts at: 299 503 1311 1725 1973 2008 299
Size: 204 808 414 248 35 2586
Fragments arranged by size:
2586 808 414 248 204 35
BanII G_rGCy'C
Cuts at: 500 1309 2339 500
Size: 809 1030 2456
Fragments arranged by size:
2456 1030 809
BbeI G_GCGC'C
Cuts at: 1729 1977 1729
Size: 248 4047
BbsI GAAGACnn'nnnn_
Cuts at: 277 388 277
Size: 111 4184
BcgI CGAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 244 278 355 389 244
Size: 34 77 34 4150
Fragments arranged by size:
4150 77 34 34
BciVI GTATCCnnnnn_n'
Cuts at: 2193 2954 4295 2193
Size: 761 1341 2193
Fragments arranged by size:
2193 1341 761
BclI T'GATC_A
Cuts at: 1815 1815
Size: 4295
BglII A'GATC_T
Cuts at: 522 1140 1810 3847 522
Size: 618 670 2037 970
Fragments arranged by size:
2037 970 670 618
BlpI GC'TnA_GC
Cuts at: 1407 1407
Size: 4295
Bme1580I G_kGCm'C
Cuts at: 241 1920 2013 3069 241
Size: 1679 93 1056 1467
Fragments arranged by size:
1679 1467 1056 93
BmgBI CAC'GTC
Cuts at: 510 603 510
Size: 93 4202
BmrI ACTGGGnnnn_n'
Cuts at: 1507 1588 1923 4067 1507
Size: 81 335 2144 1735
Fragments arranged by size:
2144 1735 335 81
BplI GAGnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 650 749 2662 650
Size: 99 1913 2283
Fragments arranged by size:
2283 1913 99
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 266 377 266
Size: 111 4184
Bpu10I CC'TnA_GC
Cuts at: 589 589
Size: 4295
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 1121 1132 1257 1471 2350 2842 3140 1121
Size: 11 125 214 879 492 298 2276
Fragments arranged by size:
2276 879 492 298 214 125 11
BsaI GGTCTCn'nnnn_
Cuts at: 169 242 353 3999 169
Size: 73 111 3646 465
Fragments arranged by size:
3646 465 111 73
BsaAI yAC'GTr
Cuts at: 1648 1648
Size: 4295
BsaBI GATnn'nnATC
Cuts at: 6 1809 3846 6
Size: 1803 2037 455
Fragments arranged by size:
2037 1803 455
BsaHI Gr'CG_yC
Cuts at: 1726 1974 2676 4172 1726
Size: 248 702 1496 1849
Fragments arranged by size:
1849 1496 702 248
BsaWI w'CCGG_w
Cuts at: 1484 2005 2683 2957 3104 3515 1484
Size: 521 678 274 147 411 2264
Fragments arranged by size:
2264 678 521 411 274 147
BsaXI ACnnnnnCTCCnnnnnnn_nnn'
Cuts at: 487 517 487
Size: 30 4265
BseYI C'CCAG_C
Cuts at: 1720 2478 3055 3551 1720
Size: 758 577 496 2464
Fragments arranged by size:
2464 758 577 496
BsgI GTGCAGnnnnnnnnnnnnnn_nn'
Cuts at: 850 850
Size: 4295
BsiEI CG_ry'CG
Cuts at: 104 1837 1883 3091 104
Size: 1733 46 1208 1308
Fragments arranged by size:
1733 1308 1208 46
BsiHKAI G_wGCw'C
Cuts at: 1309 2087 2277 3069 1309
Size: 778 190 792 2535
Fragments arranged by size:
2535 792 778 190
Bsp1286I G_dGCh'C
Cuts at: 241 500 1309 1920 2013 2087 2277 2339 3069
Size: 259 809 611 93 74 190 62 730
Cuts at: 3069 241
Size: 1467
Fragments arranged by size:
1467 809 730 611 259 190 93 74
62
BspEI T'CCGG_A
Cuts at: 3515 3515
Size: 4295
BspHI T'CATG_A
Cuts at: 3471 3471
Size: 4295
BspMI ACCTGCnnnn'nnnn_
Cuts at: 1345 1559 1861 2242 1345
Size: 214 302 381 3398
Fragments arranged by size:
3398 381 302 214
BsrBI CCG'CTC
Cuts at: 50 221 443 2587 2641 4125 4290 50
Size: 171 222 2144 54 1484 165 55
Fragments arranged by size:
2144 1484 222 171 165 55 54
BsrDI GCAATG_nn'
Cuts at: 2207 2207
Size: 4295
BsrFI r'CCGG_y
Cuts at: 132 2293 2474 2683 132
Size: 2161 181 209 1744
Fragments arranged by size:
2161 1744 209 181
BsrGI T'GTAC_A
Cuts at: 975 975
Size: 4295
BssSI C'ACGA_G
Cuts at: 2566 2924 2566
Size: 358 3937
BstAPI GCAn_nnn'nTGC
Cuts at: 188 299 410 1672 188
Size: 111 111 1262 2811
Fragments arranged by size:
2811 1262 111 111
BstBI TT'CG_AA
Cuts at: 2656 2656
Size: 4295
BstXI CCAn_nnnn'nTGG
Cuts at: 514 514
Size: 4295
BstYI r'GATC_y
Cuts at: 1 522 573 1140 1320 1787 1810 2145 2391
Size: 521 51 567 180 467 23 335 246
Cuts at: 2391 3403 3489 3501 3847 1
Size: 1012 86 12 346 449
Fragments arranged by size:
1012 567 521 467 449 346 335 246
180 86 51 23 12
BtgI C'CryG_G
Cuts at: 107 513 107
Size: 406 3889
BtgZI GCGATGnnnnnnnnnn'nnnn_
Cuts at: 2245 2426 2589 2245
Size: 181 163 3951
Fragments arranged by size:
3951 181 163
BtsI GCAGTG_nn'
Cuts at: 306 417 907 1538 306
Size: 111 490 631 3063
Fragments arranged by size:
3063 631 490 111
DraI TTT'AAA
Cuts at: 1031 1294 1031
Size: 263 4032
DrdI GACnn_nn'nnGTC
Cuts at: 2001 2859 2001
Size: 858 3437
EaeI y'GGCC_r
Cuts at: 899 1157 1880 2054 2445 2472 3817 899
Size: 258 723 174 391 27 1345 1377
Fragments arranged by size:
1377 1345 723 391 258 174 27
EagI C'GGCC_G
Cuts at: 1880 1880
Size: 4295
EarI CTCTTCn'nnn_
Cuts at: 147 1248 2318 2528 147
Size: 1101 1070 210 1914
Fragments arranged by size:
1914 1101 1070 210
EciI GGCGGAnnnnnnnnn_nn'
Cuts at: 2503 2813 2959 4105 2503
Size: 310 146 1146 2693
Fragments arranged by size:
2693 1146 310 146
EcoICRI GAG'CTC
Cuts at: 1307 1307
Size: 4295
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 266 377 2120 2552 3299 3567 266
Size: 111 1743 432 747 268 994
Fragments arranged by size:
1743 994 747 432 268 111
EcoNI CCTnn'n_nnAGG
Cuts at: 210 321 432 210
Size: 111 111 4073
Fragments arranged by size:
4073 111 111
EcoO109I rG'GnC_Cy
Cuts at: 1434 1434
Size: 4295
EcoRI G'AATT_C
Cuts at: 660 1299 660
Size: 639 3656
EcoRV GAT'ATC
Cuts at: 930 930
Size: 4295
FalI AAGnnnnnCTTnnnnnnnn_nnnnn'
Cuts at: 2314 2314
Size: 4295
FspI TGC'GCA
Cuts at: 2076 2076
Size: 4295
HaeII r_GCGC'y
Cuts at: 353 464 488 1729 1977 2999 3978 353
Size: 111 24 1241 248 1022 979 670
Fragments arranged by size:
1241 1022 979 670 248 111 24
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 919 951 2137 2167 2169 2199 919
Size: 32 1186 30 2 30 3015
Fragments arranged by size:
3015 1186 32 30 30 2
HincII GTy'rAC
Cuts at: 700 1334 700
Size: 634 3661
KasI G'GCGC_C
Cuts at: 1725 1973 1725
Size: 248 4047
KpnI G_GTAC'C
Cuts at: 507 1315 507
Size: 808 3487
MluI A'CGCG_T
Cuts at: 3728 3728
Size: 4295
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 2966 3150 2966
Size: 184 4111
MscI TGG'CCA
Cuts at: 901 2056 901
Size: 1155 3140
MslI CAynn'nnrTG
Cuts at: 512 583 601 1187 2284 2411 512
Size: 71 18 586 1097 127 2396
Fragments arranged by size:
2396 1097 586 127 71 18
MspA1I CmG'CkG
Cuts at: 1406 1720 2080 3093 3338 1406
Size: 314 360 1013 245 2363
Fragments arranged by size:
2363 1013 360 314 245
NaeI GCC'GGC
Cuts at: 134 2476 134
Size: 2342 1953
NarI GG'CG_CC
Cuts at: 1726 1974 1726
Size: 248 4047
NcoI C'CATG_G
Cuts at: 107 107
Size: 4295
NgoMIV G'CCGG_C
Cuts at: 132 2474 132
Size: 2342 1953
NspI r_CATG'y
Cuts at: 1348 1848 2755 1348
Size: 500 907 2888
Fragments arranged by size:
2888 907 500
PciI A'CATG_T
Cuts at: 2751 2751
Size: 4295
PmeI GTTT'AAAC
Cuts at: 1294 1294
Size: 4295
PpiI GAACnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 3434 3466 3434
Size: 32 4263
PstI C_TGCA'G
Cuts at: 325 436 1342 2027 325
Size: 111 906 685 2593
Fragments arranged by size:
2593 906 685 111
PvuI CG_AT'CG
Cuts at: 104 104
Size: 4295
PvuII CAG'CTG
Cuts at: 1720 2080 1720
Size: 360 3935
RsrII CG'GwC_CG
Cuts at: 2490 2490
Size: 4295
SacI G_AGCT'C
Cuts at: 1309 1309
Size: 4295
SalI G'TCGA_C
Cuts at: 1332 1332
Size: 4295
SapI GCTCTTCn'nnn_
Cuts at: 2318 2528 2318
Size: 210 4085
SbfI CC_TGCA'GG
Cuts at: 1342 1342
Size: 4295
ScaI AGT'ACT
Cuts at: 3812 3812
Size: 4295
SfcI C'TryA_G
Cuts at: 33 210 321 432 1338 2023 3016 3207 33
Size: 177 111 111 906 685 993 191 1121
Fragments arranged by size:
1121 993 906 685 191 177 111 111
SfoI GGC'GCC
Cuts at: 1727 1975 1727
Size: 248 4047
SgfI GCG_AT'CGC
Cuts at: 104 104
Size: 4295
SmaI CCC'GGG
Cuts at: 1317 1317
Size: 4295
SmlI C'TyrA_G
Cuts at: 1111 1136 1272 1450 2365 2857 3119 1111
Size: 25 136 178 915 492 262 2287
Fragments arranged by size:
2287 915 492 262 178 136 25
SpeI A'CTAG_T
Cuts at: 67 67
Size: 4295
SphI G_CATG'C
Cuts at: 1348 1848 1348
Size: 500 3795
SspI AAT'ATT
Cuts at: 837 1122 837
Size: 285 4010
StyI C'CwwG_G
Cuts at: 107 1429 1783 107
Size: 1322 354 2619
Fragments arranged by size:
2619 1322 354
SwaI ATTT'AAAT
Cuts at: 1031 1031
Size: 4295
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 1878 2011 2470 2679 1878
Size: 133 459 209 3494
Fragments arranged by size:
3494 459 209 133
TatI w'GTAC_w
Cuts at: 975 3810 975
Size: 2835 1460
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 3812 3844 3812
Size: 32 4263
Tth111I GACn'n_nGTC
Cuts at: 695 2092 695
Size: 1397 2898
XbaI T'CTAG_A
Cuts at: 1326 1326
Size: 4295
XcmI CCAnnnn_n'nnnnTGG
Cuts at: 1790 1790
Size: 4295
XmaI C'CCGG_G
Cuts at: 1315 1315
Size: 4295
Enzymes that do cut:
AccI Acc65I AclI AcuI AflIII AgeI AleI AlwNI
ApaLI ApoI AseI AsiSI AvaI BamHI BanI BanII
BbeI BbsI BcgI BciVI BclI BglII BlpI Bme1580I
BmgBI BmrI BplI BpmI Bpu10I BpuEI BsaI BsaAI
BsaBI BsaHI BsaWI BsaXI BseYI BsgI BsiEI BsiHKAI
Bsp1286I BspEI BspHI BspMI BsrBI BsrDI BsrFI BsrGI
BssSI BstAPI BstBI BstXI BstYI BtgI BtgZI BtsI
DraI DrdI EaeI EagI EarI EciI EcoICRI Eco57MI
EcoNI EcoO109I EcoRI EcoRV FalI FspI HaeII Hin4I
HincII KasI KpnI MluI MmeI MscI MslI MspA1I
NaeI NarI NcoI NgoMIV NspI PciI PmeI PpiI
PstI PvuI PvuII RsrII SacI SalI SapI SbfI
ScaI SfcI SfoI SgfI SmaI SmlI SpeI SphI
SspI StyI SwaI TaqII TatI TstI Tth111I XbaI
XcmI XmaI
Enzymes that do not cut:
AarI AatII AfeI AflII AhdI AlfI AloI ApaI
AscI AvrII BaeI BbvCI BfrBI BglI BmtI BseRI
BsiWI BsmI BsmBI BssHII BstEII BstZ17I Bsu36I ClaI
CspCI DraIII FseI FspAI HindIII HpaI MfeI NdeI
NheI NotI NruI NsiI PacI PasI PflMI PfoI
PmlI PpuMI PshAI PsiI PspOMI PspXI PsrI SacII
SanDI SexAI SfiI SgrAI SnaBI SrfI StuI XhoI
XmnI ZraI