Restriction map for
pF1KB7383
(Circular) (Six-base) MAPSORT of: pF1KB7383.seq Check: 6595 from: 1 to: 4163
pF1KB7383 4163 bp
With 182 enzymes: SgfI *
October 7, 2009 14:23 ..
AarI CACCTGCnnnn'nnnn_
Cuts at: 585 981 585
Size: 396 3767
AccI GT'mk_AC
Cuts at: 390 1201 390
Size: 811 3352
Acc65I G'GTAC_C
Cuts at: 1179 1179
Size: 4163
AclI AA'CG_TT
Cuts at: 1157 4003 1157
Size: 2846 1317
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 331 767 1988 2420 3167 3435 331
Size: 436 1221 432 747 268 1059
Fragments arranged by size:
1221 1059 747 436 432 268
AflIII A'CryG_T
Cuts at: 1515 2619 3596 1515
Size: 1104 977 2082
Fragments arranged by size:
2082 1104 977
AgeI A'CCGG_T
Cuts at: 2551 2551
Size: 4163
AlwNI CAG_nnn'CTG
Cuts at: 1468 3035 1468
Size: 1567 2596
ApaLI G'TGCA_C
Cuts at: 2933 2933
Size: 4163
ApoI r'AATT_y
Cuts at: 265 1167 3516 3772 265
Size: 902 2349 256 656
Fragments arranged by size:
2349 902 656 256
AseI AT'TA_AT
Cuts at: 20 20
Size: 4163
AsiSI GCG_AT'CGC
Cuts at: 104 104
Size: 4163
AvaI C'yCGr_G
Cuts at: 1033 1102 1183 1033
Size: 69 81 4013
Fragments arranged by size:
4013 81 69
BamHI G'GATC_C
Cuts at: 346 1188 346
Size: 842 3321
BanI G'GyrC_C
Cuts at: 1179 1593 1841 1876 1179
Size: 414 248 35 3466
Fragments arranged by size:
3466 414 248 35
BanII G_rGCy'C
Cuts at: 1177 2207 1177
Size: 1030 3133
BbeI G_GCGC'C
Cuts at: 1597 1845 1597
Size: 248 3915
BbsI GAAGACnn'nnnn_
Cuts at: 319 425 535 319
Size: 106 110 3947
Fragments arranged by size:
3947 110 106
BbvCI CC'TCA_GC
Cuts at: 936 948 1005 1062 1074 1133 936
Size: 12 57 57 12 59 3966
Fragments arranged by size:
3966 59 57 57 12 12
BciVI GTATCCnnnnn_n'
Cuts at: 2061 2822 4163 2061
Size: 761 1341 2061
Fragments arranged by size:
2061 1341 761
BclI T'GATC_A
Cuts at: 1683 1683
Size: 4163
BglI GCCn_nnn'nGGC
Cuts at: 798 798
Size: 4163
BglII A'GATC_T
Cuts at: 1678 3715 1678
Size: 2037 2126
BlpI GC'TnA_GC
Cuts at: 136 1275 136
Size: 1139 3024
Bme1580I G_kGCm'C
Cuts at: 1788 1881 2937 1788
Size: 93 1056 3014
Fragments arranged by size:
3014 1056 93
BmrI ACTGGGnnnn_n'
Cuts at: 1375 1456 1791 3935 1375
Size: 81 335 2144 1603
Fragments arranged by size:
2144 1603 335 81
BplI GAGnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 2530 2530
Size: 4163
Bpu10I CC'TnA_GC
Cuts at: 120 889 936 948 1005 1062 1074 1133 120
Size: 769 47 12 57 57 12 59 3150
Fragments arranged by size:
3150 769 59 57 57 47 12 12
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 880 949 1006 1075 1134 1339 2218 2710 3008
Size: 69 57 69 59 205 879 492 298
Cuts at: 3008 880
Size: 2035
Fragments arranged by size:
2035 879 492 298 205 69 69 59
57
BsaI GGTCTCn'nnnn_
Cuts at: 3867 3867
Size: 4163
BsaAI yAC'GTr
Cuts at: 1516 1516
Size: 4163
BsaBI GATnn'nnATC
Cuts at: 6 959 1016 1085 1144 1677 3714 6
Size: 953 57 69 59 533 2037 455
Fragments arranged by size:
2037 953 533 455 69 59 57
BsaHI Gr'CG_yC
Cuts at: 1594 1842 2544 4040 1594
Size: 248 702 1496 1717
Fragments arranged by size:
1717 1496 702 248
BsaWI w'CCGG_w
Cuts at: 1352 1873 2551 2825 2972 3383 1352
Size: 521 678 274 147 411 2132
Fragments arranged by size:
2132 678 521 411 274 147
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 842 842
Size: 4163
BseYI C'CCAG_C
Cuts at: 1588 2346 2923 3419 1588
Size: 758 577 496 2332
Fragments arranged by size:
2332 758 577 496
BsgI GTGCAGnnnnnnnnnnnnnn_nn'
Cuts at: 267 267
Size: 4163
BsiEI CG_ry'CG
Cuts at: 104 1705 1751 2959 104
Size: 1601 46 1208 1308
Fragments arranged by size:
1601 1308 1208 46
BsiHKAI G_wGCw'C
Cuts at: 578 1177 1955 2145 2937 578
Size: 599 778 190 792 1804
Fragments arranged by size:
1804 792 778 599 190
BsmBI CGTCTCn'nnnn_
Cuts at: 332 332
Size: 4163
Bsp1286I G_dGCh'C
Cuts at: 578 1177 1788 1881 1955 2145 2207 2937 578
Size: 599 611 93 74 190 62 730 1804
Fragments arranged by size:
1804 730 611 599 190 93 74 62
BspEI T'CCGG_A
Cuts at: 3383 3383
Size: 4163
BspHI T'CATG_A
Cuts at: 504 3339 504
Size: 2835 1328
BspMI ACCTGCnnnn'nnnn_
Cuts at: 585 981 1213 1427 1729 2110 585
Size: 396 232 214 302 381 2638
Fragments arranged by size:
2638 396 381 302 232 214
BsrBI CCG'CTC
Cuts at: 50 140 2455 2509 3993 4158 50
Size: 90 2315 54 1484 165 55
Fragments arranged by size:
2315 1484 165 90 55 54
BsrDI GCAATG_nn'
Cuts at: 2075 2075
Size: 4163
BsrFI r'CCGG_y
Cuts at: 2161 2342 2551 2161
Size: 181 209 3773
Fragments arranged by size:
3773 209 181
BsrGI T'GTAC_A
Cuts at: 357 357
Size: 4163
BssSI C'ACGA_G
Cuts at: 2434 2792 2434
Size: 358 3805
BstAPI GCAn_nnn'nTGC
Cuts at: 678 1540 678
Size: 862 3301
BstBI TT'CG_AA
Cuts at: 2524 2524
Size: 4163
BstYI r'GATC_y
Cuts at: 1 346 1188 1655 1678 2013 2259 3271 3357
Size: 345 842 467 23 335 246 1012 86
Cuts at: 3357 3369 3715 1
Size: 12 346 449
Fragments arranged by size:
1012 842 467 449 346 345 335 246
86 23 12
BtgI C'CryG_G
Cuts at: 350 350
Size: 4163
BtgZI GCGATGnnnnnnnnnn'nnnn_
Cuts at: 2113 2294 2457 2113
Size: 181 163 3819
Fragments arranged by size:
3819 181 163
BtsI GCAGTG_nn'
Cuts at: 770 1406 770
Size: 636 3527
DraI TTT'AAA
Cuts at: 824 1162 824
Size: 338 3825
DrdI GACnn_nn'nnGTC
Cuts at: 1869 2727 1869
Size: 858 3305
EaeI y'GGCC_r
Cuts at: 1748 1922 2313 2340 3685 1748
Size: 174 391 27 1345 2226
Fragments arranged by size:
2226 1345 391 174 27
EagI C'GGCC_G
Cuts at: 1748 1748
Size: 4163
EarI CTCTTCn'nnn_
Cuts at: 2186 2396 2186
Size: 210 3953
EciI GGCGGAnnnnnnnnn_nn'
Cuts at: 2371 2681 2827 3973 2371
Size: 310 146 1146 2561
Fragments arranged by size:
2561 1146 310 146
EcoICRI GAG'CTC
Cuts at: 1175 1175
Size: 4163
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 331 767 1988 2420 3167 3435 331
Size: 436 1221 432 747 268 1059
Fragments arranged by size:
1221 1059 747 436 432 268
EcoO109I rG'GnC_Cy
Cuts at: 1302 1302
Size: 4163
EcoRI G'AATT_C
Cuts at: 1167 1167
Size: 4163
FalI AAGnnnnnCTTnnnnnnnn_nnnnn'
Cuts at: 336 2182 336
Size: 1846 2317
FspI TGC'GCA
Cuts at: 671 1944 671
Size: 1273 2890
HaeII r_GCGC'y
Cuts at: 1597 1845 2867 3846 1597
Size: 248 1022 979 1914
Fragments arranged by size:
1914 1022 979 248
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 452 484 698 730 2005 2035 2037 2067 452
Size: 32 214 32 1275 30 2 30 2548
Fragments arranged by size:
2548 1275 214 32 32 30 30 2
HincII GTy'rAC
Cuts at: 498 1202 498
Size: 704 3459
KasI G'GCGC_C
Cuts at: 1593 1841 1593
Size: 248 3915
KpnI G_GTAC'C
Cuts at: 1183 1183
Size: 4163
MluI A'CGCG_T
Cuts at: 3596 3596
Size: 4163
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 404 880 2834 3018 404
Size: 476 1954 184 1549
Fragments arranged by size:
1954 1549 476 184
MscI TGG'CCA
Cuts at: 1924 1924
Size: 4163
MslI CAynn'nnrTG
Cuts at: 244 649 655 2152 2279 244
Size: 405 6 1497 127 2128
Fragments arranged by size:
2128 1497 405 127 6
MspA1I CmG'CkG
Cuts at: 814 952 1009 1078 1137 1274 1588 1948 2961
Size: 138 57 69 59 137 314 360 1013
Cuts at: 2961 3206 814
Size: 245 1771
Fragments arranged by size:
1771 1013 360 314 245 138 137 69
59 57
NaeI GCC'GGC
Cuts at: 2344 2344
Size: 4163
NarI GG'CG_CC
Cuts at: 1594 1842 1594
Size: 248 3915
NgoMIV G'CCGG_C
Cuts at: 2342 2342
Size: 4163
NspI r_CATG'y
Cuts at: 1216 1716 2623 1216
Size: 500 907 2756
Fragments arranged by size:
2756 907 500
PciI A'CATG_T
Cuts at: 2619 2619
Size: 4163
PfoI T'CCnGG_A
Cuts at: 413 413
Size: 4163
PmeI GTTT'AAAC
Cuts at: 1162 1162
Size: 4163
PpiI GAACnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 3302 3334 3302
Size: 32 4131
PshAI GACnn'nnGTC
Cuts at: 194 194
Size: 4163
PsiI TTA'TAA
Cuts at: 263 263
Size: 4163
PstI C_TGCA'G
Cuts at: 1210 1895 1210
Size: 685 3478
PvuI CG_AT'CG
Cuts at: 104 104
Size: 4163
PvuII CAG'CTG
Cuts at: 814 1588 1948 814
Size: 774 360 3029
Fragments arranged by size:
3029 774 360
RsrII CG'GwC_CG
Cuts at: 2358 2358
Size: 4163
SacI G_AGCT'C
Cuts at: 1177 1177
Size: 4163
SalI G'TCGA_C
Cuts at: 1200 1200
Size: 4163
SapI GCTCTTCn'nnn_
Cuts at: 2186 2396 2186
Size: 210 3953
SbfI CC_TGCA'GG
Cuts at: 1210 1210
Size: 4163
ScaI AGT'ACT
Cuts at: 3680 3680
Size: 4163
SfcI C'TryA_G
Cuts at: 33 702 1206 1891 2884 3075 33
Size: 669 504 685 993 191 1121
Fragments arranged by size:
1121 993 685 669 504 191
SfoI GGC'GCC
Cuts at: 1595 1843 1595
Size: 248 3915
SgfI GCG_AT'CGC
Cuts at: 104 104
Size: 4163
SmaI CCC'GGG
Cuts at: 1185 1185
Size: 4163
SmlI C'TyrA_G
Cuts at: 895 964 1021 1090 1149 1318 2233 2725 2987
Size: 69 57 69 59 169 915 492 262
Cuts at: 2987 895
Size: 2071
Fragments arranged by size:
2071 915 492 262 169 69 69 59
57
SpeI A'CTAG_T
Cuts at: 67 67
Size: 4163
SphI G_CATG'C
Cuts at: 1216 1716 1216
Size: 500 3663
StyI C'CwwG_G
Cuts at: 226 1297 1651 226
Size: 1071 354 2738
Fragments arranged by size:
2738 1071 354
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 1746 1879 2338 2547 1746
Size: 133 459 209 3362
Fragments arranged by size:
3362 459 209 133
TatI w'GTAC_w
Cuts at: 357 3678 357
Size: 3321 842
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 912 944 1038 1070 1109 1141 3680 3712 912
Size: 32 94 32 39 32 2539 32 1363
Fragments arranged by size:
2539 1363 94 39 32 32 32 32
Tth111I GACn'n_nGTC
Cuts at: 1960 1960
Size: 4163
XbaI T'CTAG_A
Cuts at: 1194 1194
Size: 4163
XcmI CCAnnnn_n'nnnnTGG
Cuts at: 199 1658 199
Size: 1459 2704
XmaI C'CCGG_G
Cuts at: 1183 1183
Size: 4163
XmnI GAAnn'nnTTC
Cuts at: 659 659
Size: 4163
Enzymes that do cut:
AarI AccI Acc65I AclI AcuI AflIII AgeI AlwNI
ApaLI ApoI AseI AsiSI AvaI BamHI BanI BanII
BbeI BbsI BbvCI BciVI BclI BglI BglII BlpI
Bme1580I BmrI BplI Bpu10I BpuEI BsaI BsaAI BsaBI
BsaHI BsaWI BseRI BseYI BsgI BsiEI BsiHKAI BsmBI
Bsp1286I BspEI BspHI BspMI BsrBI BsrDI BsrFI BsrGI
BssSI BstAPI BstBI BstYI BtgI BtgZI BtsI DraI
DrdI EaeI EagI EarI EciI EcoICRI Eco57MI EcoO109I
EcoRI FalI FspI HaeII Hin4I HincII KasI KpnI
MluI MmeI MscI MslI MspA1I NaeI NarI NgoMIV
NspI PciI PfoI PmeI PpiI PshAI PsiI PstI
PvuI PvuII RsrII SacI SalI SapI SbfI ScaI
SfcI SfoI SgfI SmaI SmlI SpeI SphI StyI
TaqII TatI TstI Tth111I XbaI XcmI XmaI XmnI
Enzymes that do not cut:
AatII AfeI AflII AhdI AleI AlfI AloI ApaI
AscI AvrII BaeI BcgI BfrBI BmgBI BmtI BpmI
BsaXI BsiWI BsmI BssHII BstEII BstXI BstZ17I Bsu36I
ClaI CspCI DraIII EcoNI EcoRV FseI FspAI HindIII
HpaI MfeI NcoI NdeI NheI NotI NruI NsiI
PacI PasI PflMI PmlI PpuMI PspOMI PspXI PsrI
SacII SanDI SexAI SfiI SgrAI SnaBI SrfI SspI
StuI SwaI XhoI ZraI