Restriction map for
pF1KB7352
(Circular) (Six-base) MAPSORT of: pF1KB7352.seq Check: 4158 from: 1 to: 5642
pF1KB7352 5642 bp
With 182 enzymes: SgfI *
April 24, 2009 13:08 ..
AarI CACCTGCnnnn'nnnn_
Cuts at: 2472 2472
Size: 5642
AccI GT'mk_AC
Cuts at: 1043 2680 1043
Size: 1637 4005
Acc65I G'GTAC_C
Cuts at: 1307 2658 1307
Size: 1351 4291
AclI AA'CG_TT
Cuts at: 5482 5482
Size: 5642
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 298 757 1405 1717 2040 2237 3467 3899 4646
Size: 459 648 312 323 197 1230 432 747
Cuts at: 4646 4914 298
Size: 268 1026
Fragments arranged by size:
1230 1026 747 648 459 432 323 312
268 197
AfeI AGC'GCT
Cuts at: 154 154
Size: 5642
AflII C'TTAA_G
Cuts at: 379 379
Size: 5642
AflIII A'CryG_T
Cuts at: 2994 4098 5075 2994
Size: 1104 977 3561
Fragments arranged by size:
3561 1104 977
AgeI A'CCGG_T
Cuts at: 4030 4030
Size: 5642
AleI CACnn'nnGTG
Cuts at: 1163 2395 1163
Size: 1232 4410
AlfI GCAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 1787 2168 1787
Size: 381 5261
AlwNI CAG_nnn'CTG
Cuts at: 276 2121 2415 2947 4514 276
Size: 1845 294 532 1567 1404
Fragments arranged by size:
1845 1567 1404 532 294
ApaI G_GGCC'C
Cuts at: 1629 2170 1629
Size: 541 5101
ApaLI G'TGCA_C
Cuts at: 4412 4412
Size: 5642
ApoI r'AATT_y
Cuts at: 388 1294 1851 2646 4995 5251 388
Size: 906 557 795 2349 256 779
Fragments arranged by size:
2349 906 795 779 557 256
AseI AT'TA_AT
Cuts at: 20 20
Size: 5642
AsiSI GCG_AT'CGC
Cuts at: 104 104
Size: 5642
AvaI C'yCGr_G
Cuts at: 250 311 470 2065 2662 250
Size: 61 159 1595 597 3230
Fragments arranged by size:
3230 1595 597 159 61
BaeI ACnnnnGTAyCnnnnnnn_nnnnn'
Cuts at: 646 679 646
Size: 33 5609
BamHI G'GATC_C
Cuts at: 182 957 2667 182
Size: 775 1710 3157
Fragments arranged by size:
3157 1710 775
BanI G'GyrC_C
Cuts at: 1307 2658 3072 3320 3355 1307
Size: 1351 414 248 35 3594
Fragments arranged by size:
3594 1351 414 248 35
BanII G_rGCy'C
Cuts at: 194 783 1038 1438 1541 1629 2170 2333 2656
Size: 589 255 400 103 88 541 163 323
Cuts at: 2656 3686 194
Size: 1030 2150
Fragments arranged by size:
2150 1030 589 541 400 323 255 163
103 88
BbeI G_GCGC'C
Cuts at: 3076 3324 3076
Size: 248 5394
BciVI GTATCCnnnnn_n'
Cuts at: 1142 1387 3540 4301 5642 1142
Size: 245 2153 761 1341 1142
Fragments arranged by size:
2153 1341 1142 761 245
BclI T'GATC_A
Cuts at: 410 3162 410
Size: 2752 2890
BglI GCCn_nnn'nGGC
Cuts at: 516 516
Size: 5642
BglII A'GATC_T
Cuts at: 908 2198 3157 5194 908
Size: 1290 959 2037 1356
Fragments arranged by size:
2037 1356 1290 959
BlpI GC'TnA_GC
Cuts at: 1141 2327 2754 1141
Size: 1186 427 4029
Fragments arranged by size:
4029 1186 427
Bme1580I G_kGCm'C
Cuts at: 1629 2157 2170 2612 3267 3360 4416 1629
Size: 528 13 442 655 93 1056 2855
Fragments arranged by size:
2855 1056 655 528 442 93 13
BmgBI CAC'GTC
Cuts at: 1987 1987
Size: 5642
BmrI ACTGGGnnnn_n'
Cuts at: 1967 2854 2935 3270 5414 1967
Size: 887 81 335 2144 2195
Fragments arranged by size:
2195 2144 887 335 81
BplI GAGnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 859 2091 4009 859
Size: 1232 1918 2492
Fragments arranged by size:
2492 1918 1232
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 157 286 973 1674 2608 157
Size: 129 687 701 934 3191
Fragments arranged by size:
3191 934 701 687 129
Bpu10I CC'TnA_GC
Cuts at: 188 716 2159 2376 2537 188
Size: 528 1443 217 161 3293
Fragments arranged by size:
3293 1443 528 217 161
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 1055 2088 2818 3697 4189 4487 1055
Size: 1033 730 879 492 298 2210
Fragments arranged by size:
2210 1033 879 730 492 298
BsaI GGTCTCn'nnnn_
Cuts at: 5346 5346
Size: 5642
BsaAI yAC'GTr
Cuts at: 1544 2397 2995 1544
Size: 853 598 4191
Fragments arranged by size:
4191 853 598
BsaBI GATnn'nnATC
Cuts at: 6 3156 5193 6
Size: 3150 2037 455
Fragments arranged by size:
3150 2037 455
BsaHI Gr'CG_yC
Cuts at: 3073 3321 4023 5519 3073
Size: 248 702 1496 3196
Fragments arranged by size:
3196 1496 702 248
BsaWI w'CCGG_w
Cuts at: 2831 3352 4030 4304 4451 4862 2831
Size: 521 678 274 147 411 3611
Fragments arranged by size:
3611 678 521 411 274 147
BsaXI ACnnnnnCTCCnnnnnnn_nnn'
Cuts at: 1023 1053 1023
Size: 30 5612
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 238 833 1402 238
Size: 595 569 4478
Fragments arranged by size:
4478 595 569
BseYI C'CCAG_C
Cuts at: 326 2517 3067 3825 4402 4898 326
Size: 2191 550 758 577 496 1070
Fragments arranged by size:
2191 1070 758 577 550 496
BsgI GTGCAGnnnnnnnnnnnnnn_nn'
Cuts at: 1426 1747 2158 2500 1426
Size: 321 411 342 4568
Fragments arranged by size:
4568 411 342 321
BsiEI CG_ry'CG
Cuts at: 104 3184 3230 4438 104
Size: 3080 46 1208 1308
Fragments arranged by size:
3080 1308 1208 46
BsiHKAI G_wGCw'C
Cuts at: 194 2333 2636 2656 3434 3624 4416 194
Size: 2139 303 20 778 190 792 1420
Fragments arranged by size:
2139 1420 792 778 303 190 20
BsmBI CGTCTCn'nnnn_
Cuts at: 133 2144 133
Size: 2011 3631
Bsp1286I G_dGCh'C
Cuts at: 194 783 1038 1438 1541 1629 2157 2170 2333
Size: 589 255 400 103 88 528 13 163
Cuts at: 2333 2612 2636 2656 3267 3360 3434 3624 3686
Size: 279 24 20 611 93 74 190 62
Cuts at: 3686 4416 194
Size: 730 1420
Fragments arranged by size:
1420 730 611 589 528 400 279 255
190 163 103 93 88 74 62 24
20 13
BspEI T'CCGG_A
Cuts at: 4862 4862
Size: 5642
BspHI T'CATG_A
Cuts at: 4818 4818
Size: 5642
BspMI ACCTGCnnnn'nnnn_
Cuts at: 884 2472 2692 2906 3208 3589 884
Size: 1588 220 214 302 381 2937
Fragments arranged by size:
2937 1588 381 302 220 214
BsrBI CCG'CTC
Cuts at: 50 161 180 3934 3988 5472 5637 50
Size: 111 19 3754 54 1484 165 55
Fragments arranged by size:
3754 1484 165 111 55 54 19
BsrDI GCAATG_nn'
Cuts at: 1217 2012 3554 1217
Size: 795 1542 3305
Fragments arranged by size:
3305 1542 795
BsrFI r'CCGG_y
Cuts at: 3640 3821 4030 3640
Size: 181 209 5252
Fragments arranged by size:
5252 209 181
BssSI C'ACGA_G
Cuts at: 2500 3913 4271 2500
Size: 1413 358 3871
Fragments arranged by size:
3871 1413 358
BstAPI GCAn_nnn'nTGC
Cuts at: 1935 3019 1935
Size: 1084 4558
BstBI TT'CG_AA
Cuts at: 4003 4003
Size: 5642
BstXI CCAn_nnnn'nTGG
Cuts at: 900 900
Size: 5642
BstYI r'GATC_y
Cuts at: 1 182 908 957 1697 2198 2462 2667 3134
Size: 181 726 49 740 501 264 205 467
Cuts at: 3134 3157 3492 3738 4750 4836 4848 5194 1
Size: 23 335 246 1012 86 12 346 449
Fragments arranged by size:
1012 740 726 501 467 449 346 335
264 246 205 181 86 49 23 12
Bsu36I CC'TnA_GG
Cuts at: 292 292
Size: 5642
BtgI C'CryG_G
Cuts at: 107 107
Size: 5642
BtgZI GCGATGnnnnnnnnnn'nnnn_
Cuts at: 204 636 3592 3773 3936 204
Size: 432 2956 181 163 1910
Fragments arranged by size:
2956 1910 432 181 163
BtsI GCAGTG_nn'
Cuts at: 1912 2157 2885 1912
Size: 245 728 4669
Fragments arranged by size:
4669 728 245
DraI TTT'AAA
Cuts at: 2641 2641
Size: 5642
DrdI GACnn_nn'nnGTC
Cuts at: 3348 4206 3348
Size: 858 4784
EaeI y'GGCC_r
Cuts at: 209 416 1121 2316 3227 3401 3792 3819 5164
Size: 207 705 1195 911 174 391 27 1345
Cuts at: 5164 209
Size: 687
Fragments arranged by size:
1345 1195 911 705 687 391 207 174
27
EagI C'GGCC_G
Cuts at: 3227 3227
Size: 5642
EarI CTCTTCn'nnn_
Cuts at: 1227 1448 1736 2287 2363 2395 3665 3875 1227
Size: 221 288 551 76 32 1270 210 2994
Fragments arranged by size:
2994 1270 551 288 221 210 76 32
EciI GGCGGAnnnnnnnnn_nn'
Cuts at: 1369 1711 2470 3850 4160 4306 5452 1369
Size: 342 759 1380 310 146 1146 1559
Fragments arranged by size:
1559 1380 1146 759 342 310 146
EcoICRI GAG'CTC
Cuts at: 192 2331 2654 192
Size: 2139 323 3180
Fragments arranged by size:
3180 2139 323
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 157 286 298 757 973 1405 1674 1717 2040
Size: 129 12 459 216 432 269 43 323
Cuts at: 2040 2237 2608 3467 3899 4646 4914 157
Size: 197 371 859 432 747 268 885
Fragments arranged by size:
885 859 747 459 432 432 371 323
269 268 216 197 129 43 12
EcoNI CCTnn'n_nnAGG
Cuts at: 295 295
Size: 5642
EcoO109I rG'GnC_Cy
Cuts at: 316 1590 1625 2166 2526 2781 316
Size: 1274 35 541 360 255 3177
Fragments arranged by size:
3177 1274 541 360 255 35
EcoRI G'AATT_C
Cuts at: 388 1294 2646 388
Size: 906 1352 3384
Fragments arranged by size:
3384 1352 906
FalI AAGnnnnnCTTnnnnnnnn_nnnnn'
Cuts at: 1302 3661 1302
Size: 2359 3283
FspI TGC'GCA
Cuts at: 3423 3423
Size: 5642
HaeII r_GCGC'y
Cuts at: 156 1609 3076 3324 4346 5325 156
Size: 1453 1467 248 1022 979 473
Fragments arranged by size:
1467 1453 1022 979 473 248
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 770 802 1025 1057 1237 1269 1486 1518 1741
Size: 32 223 32 180 32 217 32 223
Cuts at: 1741 1773 3484 3514 3516 3546 770
Size: 32 1711 30 2 30 2866
Fragments arranged by size:
2866 1711 223 223 217 180 32 32
32 32 32 30 30 2
HincII GTy'rAC
Cuts at: 2681 2681
Size: 5642
KasI G'GCGC_C
Cuts at: 3072 3320 3072
Size: 248 5394
KpnI G_GTAC'C
Cuts at: 1311 2662 1311
Size: 1351 4291
MluI A'CGCG_T
Cuts at: 5075 5075
Size: 5642
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 804 1430 2105 4313 4497 804
Size: 626 675 2208 184 1949
Fragments arranged by size:
2208 1949 675 626 184
MscI TGG'CCA
Cuts at: 418 1123 3403 418
Size: 705 2280 2657
Fragments arranged by size:
2657 2280 705
MslI CAynn'nnrTG
Cuts at: 1163 1802 1825 2395 3631 3758 1163
Size: 639 23 570 1236 127 3047
Fragments arranged by size:
3047 1236 639 570 127 23
MspA1I CmG'CkG
Cuts at: 204 330 502 900 2172 2215 2753 3067 3427
Size: 126 172 398 1272 43 538 314 360
Cuts at: 3427 4440 4685 204
Size: 1013 245 1161
Fragments arranged by size:
1272 1161 1013 538 398 360 314 245
172 126 43
NaeI GCC'GGC
Cuts at: 3823 3823
Size: 5642
NarI GG'CG_CC
Cuts at: 3073 3321 3073
Size: 248 5394
NcoI C'CATG_G
Cuts at: 107 107
Size: 5642
NgoMIV G'CCGG_C
Cuts at: 3821 3821
Size: 5642
NspI r_CATG'y
Cuts at: 1935 2313 2409 2695 3195 4102 1935
Size: 378 96 286 500 907 3475
Fragments arranged by size:
3475 907 500 378 286 96
PciI A'CATG_T
Cuts at: 4098 4098
Size: 5642
PfoI T'CCnGG_A
Cuts at: 2468 2468
Size: 5642
PmeI GTTT'AAAC
Cuts at: 2641 2641
Size: 5642
PmlI CAC'GTG
Cuts at: 2397 2397
Size: 5642
PpiI GAACnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 4781 4813 4781
Size: 32 5610
PpuMI rG'GwC_Cy
Cuts at: 1590 2526 1590
Size: 936 4706
PshAI GACnn'nnGTC
Cuts at: 337 502 337
Size: 165 5477
PsiI TTA'TAA
Cuts at: 446 446
Size: 5642
PspOMI G'GGCC_C
Cuts at: 1625 2166 1625
Size: 541 5101
PstI C_TGCA'G
Cuts at: 1187 1556 1679 1940 2147 2247 2689 3374 1187
Size: 369 123 261 207 100 442 685 3455
Fragments arranged by size:
3455 685 442 369 261 207 123 100
PvuI CG_AT'CG
Cuts at: 104 104
Size: 5642
PvuII CAG'CTG
Cuts at: 204 330 502 900 2215 3067 3427 204
Size: 126 172 398 1315 852 360 2419
Fragments arranged by size:
2419 1315 852 398 360 172 126
RsrII CG'GwC_CG
Cuts at: 3837 3837
Size: 5642
SacI G_AGCT'C
Cuts at: 194 2333 2656 194
Size: 2139 323 3180
Fragments arranged by size:
3180 2139 323
SalI G'TCGA_C
Cuts at: 2679 2679
Size: 5642
SapI GCTCTTCn'nnn_
Cuts at: 1736 2395 3665 3875 1736
Size: 659 1270 210 3503
Fragments arranged by size:
3503 1270 659 210
SbfI CC_TGCA'GG
Cuts at: 2689 2689
Size: 5642
ScaI AGT'ACT
Cuts at: 5159 5159
Size: 5642
SfcI C'TryA_G
Cuts at: 33 513 1183 1552 1594 1675 1936 2143 2243
Size: 480 670 369 42 81 261 207 100
Cuts at: 2243 2445 2685 3370 4363 4554 33
Size: 202 240 685 993 191 1121
Fragments arranged by size:
1121 993 685 670 480 369 261 240
207 202 191 100 81 42
SfoI GGC'GCC
Cuts at: 3074 3322 3074
Size: 248 5394
SgfI GCG_AT'CGC
Cuts at: 104 104
Size: 5642
SmaI CCC'GGG
Cuts at: 472 2664 472
Size: 2192 3450
SmlI C'TyrA_G
Cuts at: 379 1070 2103 2797 3712 4204 4466 379
Size: 691 1033 694 915 492 262 1555
Fragments arranged by size:
1555 1033 915 694 691 492 262
SpeI A'CTAG_T
Cuts at: 67 67
Size: 5642
SphI G_CATG'C
Cuts at: 2409 2695 3195 2409
Size: 286 500 4856
Fragments arranged by size:
4856 500 286
SspI AAT'ATT
Cuts at: 699 699
Size: 5642
StuI AGG'CCT
Cuts at: 2374 2374
Size: 5642
StyI C'CwwG_G
Cuts at: 107 2579 2776 3130 107
Size: 2472 197 354 2619
Fragments arranged by size:
2619 2472 354 197
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 383 1656 2407 3225 3358 3817 4026 383
Size: 1273 751 818 133 459 209 1999
Fragments arranged by size:
1999 1273 818 751 459 209 133
TatI w'GTAC_w
Cuts at: 5157 5157
Size: 5642
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 949 981 1023 1055 5159 5191 949
Size: 32 42 32 4104 32 1400
Fragments arranged by size:
4104 1400 42 32 32 32
Tth111I GACn'n_nGTC
Cuts at: 3439 3439
Size: 5642
XbaI T'CTAG_A
Cuts at: 2673 2673
Size: 5642
XcmI CCAnnnn_n'nnnnTGG
Cuts at: 231 411 1428 3137 231
Size: 180 1017 1709 2736
Fragments arranged by size:
2736 1709 1017 180
XmaI C'CCGG_G
Cuts at: 470 2662 470
Size: 2192 3450
Enzymes that do cut:
AarI AccI Acc65I AclI AcuI AfeI AflII AflIII
AgeI AleI AlfI AlwNI ApaI ApaLI ApoI AseI
AsiSI AvaI BaeI BamHI BanI BanII BbeI BciVI
BclI BglI BglII BlpI Bme1580I BmgBI BmrI BplI
BpmI Bpu10I BpuEI BsaI BsaAI BsaBI BsaHI BsaWI
BsaXI BseRI BseYI BsgI BsiEI BsiHKAI BsmBI Bsp1286I
BspEI BspHI BspMI BsrBI BsrDI BsrFI BssSI BstAPI
BstBI BstXI BstYI Bsu36I BtgI BtgZI BtsI DraI
DrdI EaeI EagI EarI EciI EcoICRI Eco57MI EcoNI
EcoO109I EcoRI FalI FspI HaeII Hin4I HincII KasI
KpnI MluI MmeI MscI MslI MspA1I NaeI NarI
NcoI NgoMIV NspI PciI PfoI PmeI PmlI PpiI
PpuMI PshAI PsiI PspOMI PstI PvuI PvuII RsrII
SacI SalI SapI SbfI ScaI SfcI SfoI SgfI
SmaI SmlI SpeI SphI SspI StuI StyI TaqII
TatI TstI Tth111I XbaI XcmI XmaI
Enzymes that do not cut:
AatII AhdI AloI AscI AvrII BbsI BbvCI BcgI
BfrBI BmtI BsiWI BsmI BsrGI BssHII BstEII BstZ17I
ClaI CspCI DraIII EcoRV FseI FspAI HindIII HpaI
MfeI NdeI NheI NotI NruI NsiI PacI PasI
PflMI PspXI PsrI SacII SanDI SexAI SfiI SgrAI
SnaBI SrfI SwaI XhoI XmnI ZraI