Restriction map for
pF1KB7344
(Circular) (Six-base) MAPSORT of: pF1KB7344.seq Check: 1968 from: 1 to: 6260
pF1KB7344 6260 bp
With 182 enzymes: SgfI *
September 8, 2016 13:38 ..
AccI GT'mk_AC
Cuts at: 1353 2820 3298 1353
Size: 1467 478 4315
Fragments arranged by size:
4315 1467 478
Acc65I G'GTAC_C
Cuts at: 3276 3276
Size: 6260
AclI AA'CG_TT
Cuts at: 6100 6100
Size: 6260
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 108 701 2078 2668 4085 4517 5264 5532 108
Size: 593 1377 590 1417 432 747 268 836
Fragments arranged by size:
1417 1377 836 747 593 590 432 268
AflIII A'CryG_T
Cuts at: 3612 4716 5693 3612
Size: 1104 977 4179
Fragments arranged by size:
4179 1104 977
AgeI A'CCGG_T
Cuts at: 4648 4648
Size: 6260
AlfI GCAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 2047 2047
Size: 6260
AloI GAACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 985 1017 985
Size: 32 6228
AlwNI CAG_nnn'CTG
Cuts at: 1140 3565 5132 1140
Size: 2425 1567 2268
Fragments arranged by size:
2425 2268 1567
ApaLI G'TGCA_C
Cuts at: 2659 5030 2659
Size: 2371 3889
ApoI r'AATT_y
Cuts at: 178 323 612 758 938 957 963 1094 1174
Size: 145 289 146 180 19 6 131 80
Cuts at: 1174 2142 2181 2368 3264 5613 5869 178
Size: 968 39 187 896 2349 256 569
Fragments arranged by size:
2349 968 896 569 289 256 187 180
146 145 131 80 39 19 6
AseI AT'TA_AT
Cuts at: 20 413 434 1544 20
Size: 393 21 1110 4736
Fragments arranged by size:
4736 1110 393 21
AsiSI GCG_AT'CGC
Cuts at: 104 104
Size: 6260
AvaI C'yCGr_G
Cuts at: 970 2553 2929 3280 970
Size: 1583 376 351 3950
Fragments arranged by size:
3950 1583 376 351
AvrII C'CTAG_G
Cuts at: 2394 2394
Size: 6260
BamHI G'GATC_C
Cuts at: 3285 3285
Size: 6260
BanI G'GyrC_C
Cuts at: 217 3004 3049 3073 3276 3690 3938 3973 217
Size: 2787 45 24 203 414 248 35 2504
Fragments arranged by size:
2787 2504 414 248 203 45 35 24
BanII G_rGCy'C
Cuts at: 495 536 2480 3274 4304 495
Size: 41 1944 794 1030 2451
Fragments arranged by size:
2451 1944 1030 794 41
BbeI G_GCGC'C
Cuts at: 3694 3942 3694
Size: 248 6012
BbsI GAAGACnn'nnnn_
Cuts at: 299 914 2336 2849 299
Size: 615 1422 513 3710
Fragments arranged by size:
3710 1422 615 513
BciVI GTATCCnnnnn_n'
Cuts at: 2346 4158 4919 6260 2346
Size: 1812 761 1341 2346
Fragments arranged by size:
2346 1812 1341 761
BclI T'GATC_A
Cuts at: 384 3780 384
Size: 3396 2864
BfrBI ATG'CAT
Cuts at: 1985 1985
Size: 6260
BglI GCCn_nnn'nGGC
Cuts at: 3047 3047
Size: 6260
BglII A'GATC_T
Cuts at: 2037 3775 5812 2037
Size: 1738 2037 2485
Fragments arranged by size:
2485 2037 1738
BlpI GC'TnA_GC
Cuts at: 3372 3372
Size: 6260
Bme1580I G_kGCm'C
Cuts at: 2663 3078 3885 3978 5034 2663
Size: 415 807 93 1056 3889
Fragments arranged by size:
3889 1056 807 415 93
BmrI ACTGGGnnnn_n'
Cuts at: 1249 3020 3472 3553 3888 6032 1249
Size: 1771 452 81 335 2144 1477
Fragments arranged by size:
2144 1771 1477 452 335 81
BmtI G_CTAG'C
Cuts at: 1408 1408
Size: 6260
BplI GAGnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 4627 4627
Size: 6260
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 135 392 554 773 135
Size: 257 162 219 5622
Fragments arranged by size:
5622 257 219 162
Bpu10I CC'TnA_GC
Cuts at: 2576 3115 2576
Size: 539 5721
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 3436 4315 4807 5105 3436
Size: 879 492 298 4591
Fragments arranged by size:
4591 879 492 298
BsaI GGTCTCn'nnnn_
Cuts at: 5964 5964
Size: 6260
BsaAI yAC'GTr
Cuts at: 3613 3613
Size: 6260
BsaBI GATnn'nnATC
Cuts at: 6 1514 3774 5811 6
Size: 1508 2260 2037 455
Fragments arranged by size:
2260 2037 1508 455
BsaHI Gr'CG_yC
Cuts at: 2241 3691 3939 4641 6137 2241
Size: 1450 248 702 1496 2364
Fragments arranged by size:
2364 1496 1450 702 248
BsaWI w'CCGG_w
Cuts at: 3449 3970 4648 4922 5069 5480 3449
Size: 521 678 274 147 411 4229
Fragments arranged by size:
4229 678 521 411 274 147
BsaXI ACnnnnnCTCCnnnnnnn_nnn'
Cuts at: 1313 1343 1313
Size: 30 6230
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 1756 1826 2060 2063 3168 1756
Size: 70 234 3 1105 4848
Fragments arranged by size:
4848 1105 234 70 3
BseYI C'CCAG_C
Cuts at: 3064 3685 4443 5020 5516 3064
Size: 621 758 577 496 3808
Fragments arranged by size:
3808 758 621 577 496
BsgI GTGCAGnnnnnnnnnnnnnn_nn'
Cuts at: 1865 1865
Size: 6260
BsiEI CG_ry'CG
Cuts at: 104 3802 3848 5056 104
Size: 3698 46 1208 1308
Fragments arranged by size:
3698 1308 1208 46
BsiHKAI G_wGCw'C
Cuts at: 495 536 1836 2545 2663 3274 4052 4242 5034
Size: 41 1300 709 118 611 778 190 792
Cuts at: 5034 495
Size: 1721
Fragments arranged by size:
1721 1300 792 778 709 611 190 118
41
BsmI GAATG_Cn'
Cuts at: 231 1987 2105 231
Size: 1756 118 4386
Fragments arranged by size:
4386 1756 118
Bsp1286I G_dGCh'C
Cuts at: 495 536 1836 2480 2545 2663 3078 3274 3885
Size: 41 1300 644 65 118 415 196 611
Cuts at: 3885 3978 4052 4242 4304 5034 495
Size: 93 74 190 62 730 1721
Fragments arranged by size:
1721 1300 730 644 611 415 196 190
118 93 74 65 62 41
BspEI T'CCGG_A
Cuts at: 5480 5480
Size: 6260
BspHI T'CATG_A
Cuts at: 5436 5436
Size: 6260
BspMI ACCTGCnnnn'nnnn_
Cuts at: 1409 3310 3524 3826 4207 1409
Size: 1901 214 302 381 3462
Fragments arranged by size:
3462 1901 381 302 214
BsrBI CCG'CTC
Cuts at: 50 3071 4552 4606 6090 6255 50
Size: 3021 1481 54 1484 165 55
Fragments arranged by size:
3021 1484 1481 165 55 54
BsrDI GCAATG_nn'
Cuts at: 143 4172 143
Size: 4029 2231
BsrFI r'CCGG_y
Cuts at: 4258 4439 4648 4258
Size: 181 209 5870
Fragments arranged by size:
5870 209 181
BsrGI T'GTAC_A
Cuts at: 1737 1737
Size: 6260
BssSI C'ACGA_G
Cuts at: 4531 4889 4531
Size: 358 5902
BstAPI GCAn_nnn'nTGC
Cuts at: 1140 3637 1140
Size: 2497 3763
BstBI TT'CG_AA
Cuts at: 762 4621 762
Size: 3859 2401
BstXI CCAn_nnnn'nTGG
Cuts at: 3187 3187
Size: 6260
BstYI r'GATC_y
Cuts at: 1 401 2037 3285 3752 3775 4110 4356 5368
Size: 400 1636 1248 467 23 335 246 1012
Cuts at: 5368 5454 5466 5812 1
Size: 86 12 346 449
Fragments arranged by size:
1636 1248 1012 467 449 400 346 335
246 86 23 12
Bsu36I CC'TnA_GG
Cuts at: 2465 2465
Size: 6260
BtgI C'CryG_G
Cuts at: 107 107
Size: 6260
BtgZI GCGATGnnnnnnnnnn'nnnn_
Cuts at: 4210 4391 4554 4210
Size: 181 163 5916
Fragments arranged by size:
5916 181 163
BtsI GCAGTG_nn'
Cuts at: 3042 3503 3042
Size: 461 5799
DraI TTT'AAA
Cuts at: 1100 3259 1100
Size: 2159 4101
DrdI GACnn_nn'nnGTC
Cuts at: 3966 4824 3966
Size: 858 5402
EaeI y'GGCC_r
Cuts at: 798 2431 3144 3845 4019 4410 4437 5782 798
Size: 1633 713 701 174 391 27 1345 1276
Fragments arranged by size:
1633 1345 1276 713 701 391 174 27
EagI C'GGCC_G
Cuts at: 3845 3845
Size: 6260
EarI CTCTTCn'nnn_
Cuts at: 992 4283 4493 992
Size: 3291 210 2759
Fragments arranged by size:
3291 2759 210
EciI GGCGGAnnnnnnnnn_nn'
Cuts at: 3183 4468 4778 4924 6070 3183
Size: 1285 310 146 1146 3373
Fragments arranged by size:
3373 1285 1146 310 146
EcoICRI GAG'CTC
Cuts at: 493 534 3272 493
Size: 41 2738 3481
Fragments arranged by size:
3481 2738 41
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 108 135 392 554 701 773 2078 2668 4085
Size: 27 257 162 147 72 1305 590 1417
Cuts at: 4085 4517 5264 5532 108
Size: 432 747 268 836
Fragments arranged by size:
1417 1305 836 747 590 432 268 257
162 147 72 27
EcoNI CCTnn'n_nnAGG
Cuts at: 2464 2464
Size: 6260
EcoO109I rG'GnC_Cy
Cuts at: 2362 2557 3399 2362
Size: 195 842 5223
Fragments arranged by size:
5223 842 195
EcoRI G'AATT_C
Cuts at: 178 3264 178
Size: 3086 3174
FalI AAGnnnnnCTTnnnnnnnn_nnnnn'
Cuts at: 1655 4279 1655
Size: 2624 3636
FspI TGC'GCA
Cuts at: 4041 4041
Size: 6260
HaeII r_GCGC'y
Cuts at: 2732 3694 3942 4964 5943 2732
Size: 962 248 1022 979 3049
Fragments arranged by size:
3049 1022 979 962 248
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 838 870 1711 1743 1754 1769 1786 1801 2532
Size: 32 841 32 11 15 17 15 731
Cuts at: 2532 2564 2826 2858 2898 2930 4102 4132 4134
Size: 32 262 32 40 32 1172 30 2
Cuts at: 4134 4164 838
Size: 30 2934
Fragments arranged by size:
2934 1172 841 731 262 40 32 32
32 32 32 30 30 17 15 15
11 2
HincII GTy'rAC
Cuts at: 171 3299 171
Size: 3128 3132
HpaI GTT'AAC
Cuts at: 171 171
Size: 6260
KasI G'GCGC_C
Cuts at: 3690 3938 3690
Size: 248 6012
KpnI G_GTAC'C
Cuts at: 3280 3280
Size: 6260
MluI A'CGCG_T
Cuts at: 5693 5693
Size: 6260
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 2913 4931 5115 2913
Size: 2018 184 4058
Fragments arranged by size:
4058 2018 184
MscI TGG'CCA
Cuts at: 2433 3146 4021 2433
Size: 713 875 4672
Fragments arranged by size:
4672 875 713
MslI CAynn'nnrTG
Cuts at: 3055 4249 4376 3055
Size: 1194 127 4939
Fragments arranged by size:
4939 1194 127
MspA1I CmG'CkG
Cuts at: 859 1396 1849 3020 3371 3685 4045 5058 5303
Size: 537 453 1171 351 314 360 1013 245
Cuts at: 5303 859
Size: 1816
Fragments arranged by size:
1816 1171 1013 537 453 360 351 314
245
NaeI GCC'GGC
Cuts at: 4441 4441
Size: 6260
NarI GG'CG_CC
Cuts at: 3691 3939 3691
Size: 248 6012
NcoI C'CATG_G
Cuts at: 107 107
Size: 6260
NdeI CA'TA_TG
Cuts at: 396 1281 2214 396
Size: 885 933 4442
Fragments arranged by size:
4442 933 885
NgoMIV G'CCGG_C
Cuts at: 4439 4439
Size: 6260
NheI G'CTAG_C
Cuts at: 1404 1404
Size: 6260
NsiI A_TGCA'T
Cuts at: 1987 1987
Size: 6260
NspI r_CATG'y
Cuts at: 855 3313 3813 4720 855
Size: 2458 500 907 2395
Fragments arranged by size:
2458 2395 907 500
PasI CC'CwG_GG
Cuts at: 1925 2358 1925
Size: 433 5827
PciI A'CATG_T
Cuts at: 4716 4716
Size: 6260
PflMI CCAn_nnn'nTGG
Cuts at: 113 227 397 2725 113
Size: 114 170 2328 3648
Fragments arranged by size:
3648 2328 170 114
PmeI GTTT'AAAC
Cuts at: 3259 3259
Size: 6260
PpiI GAACnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 5399 5431 5399
Size: 32 6228
PpuMI rG'GwC_Cy
Cuts at: 2362 2557 2362
Size: 195 6065
PsiI TTA'TAA
Cuts at: 203 703 203
Size: 500 5760
PstI C_TGCA'G
Cuts at: 3307 3992 3307
Size: 685 5575
PvuI CG_AT'CG
Cuts at: 104 104
Size: 6260
PvuII CAG'CTG
Cuts at: 1396 1849 3685 4045 1396
Size: 453 1836 360 3611
Fragments arranged by size:
3611 1836 453 360
RsrII CG'GwC_CG
Cuts at: 4455 4455
Size: 6260
SacI G_AGCT'C
Cuts at: 495 536 3274 495
Size: 41 2738 3481
Fragments arranged by size:
3481 2738 41
SalI G'TCGA_C
Cuts at: 3297 3297
Size: 6260
SapI GCTCTTCn'nnn_
Cuts at: 4283 4493 4283
Size: 210 6050
SbfI CC_TGCA'GG
Cuts at: 3307 3307
Size: 6260
ScaI AGT'ACT
Cuts at: 1635 5777 1635
Size: 4142 2118
SexAI A'CCwGG_T
Cuts at: 2559 2559
Size: 6260
SfcI C'TryA_G
Cuts at: 33 1505 3303 3988 4981 5172 33
Size: 1472 1798 685 993 191 1121
Fragments arranged by size:
1798 1472 1121 993 685 191
SfoI GGC'GCC
Cuts at: 3692 3940 3692
Size: 248 6012
SgfI GCG_AT'CGC
Cuts at: 104 104
Size: 6260
SmaI CCC'GGG
Cuts at: 3282 3282
Size: 6260
SmlI C'TyrA_G
Cuts at: 970 3415 4330 4822 5084 970
Size: 2445 915 492 262 2146
Fragments arranged by size:
2445 2146 915 492 262
SpeI A'CTAG_T
Cuts at: 67 67
Size: 6260
SphI G_CATG'C
Cuts at: 3313 3813 3313
Size: 500 5760
SspI AAT'ATT
Cuts at: 1548 1548
Size: 6260
StuI AGG'CCT
Cuts at: 525 525
Size: 6260
StyI C'CwwG_G
Cuts at: 107 1454 1488 2394 3394 3748 107
Size: 1347 34 906 1000 354 2619
Fragments arranged by size:
2619 1347 1000 906 354 34
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 845 2510 2635 3049 3843 3976 4435 4644 845
Size: 1665 125 414 794 133 459 209 2461
Fragments arranged by size:
2461 1665 794 459 414 209 133 125
TatI w'GTAC_w
Cuts at: 1463 1633 1737 2565 5775 1463
Size: 170 104 828 3210 1948
Fragments arranged by size:
3210 1948 828 170 104
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 853 885 3006 3038 5777 5809 853
Size: 32 2121 32 2739 32 1304
Fragments arranged by size:
2739 2121 1304 32 32 32
Tth111I GACn'n_nGTC
Cuts at: 2622 4057 2622
Size: 1435 4825
XbaI T'CTAG_A
Cuts at: 528 780 3291 528
Size: 252 2511 3497
Fragments arranged by size:
3497 2511 252
XcmI CCAnnnn_n'nnnnTGG
Cuts at: 3755 3755
Size: 6260
XhoI C'TCGA_G
Cuts at: 970 970
Size: 6260
XmaI C'CCGG_G
Cuts at: 3280 3280
Size: 6260
XmnI GAAnn'nnTTC
Cuts at: 2142 2142
Size: 6260
Enzymes that do cut:
AccI Acc65I AclI AcuI AflIII AgeI AlfI AloI
AlwNI ApaLI ApoI AseI AsiSI AvaI AvrII BamHI
BanI BanII BbeI BbsI BciVI BclI BfrBI BglI
BglII BlpI Bme1580I BmrI BmtI BplI BpmI Bpu10I
BpuEI BsaI BsaAI BsaBI BsaHI BsaWI BsaXI BseRI
BseYI BsgI BsiEI BsiHKAI BsmI Bsp1286I BspEI BspHI
BspMI BsrBI BsrDI BsrFI BsrGI BssSI BstAPI BstBI
BstXI BstYI Bsu36I BtgI BtgZI BtsI DraI DrdI
EaeI EagI EarI EciI EcoICRI Eco57MI EcoNI EcoO109I
EcoRI FalI FspI HaeII Hin4I HincII HpaI KasI
KpnI MluI MmeI MscI MslI MspA1I NaeI NarI
NcoI NdeI NgoMIV NheI NsiI NspI PasI PciI
PflMI PmeI PpiI PpuMI PsiI PstI PvuI PvuII
RsrII SacI SalI SapI SbfI ScaI SexAI SfcI
SfoI SgfI SmaI SmlI SpeI SphI SspI StuI
StyI TaqII TatI TstI Tth111I XbaI XcmI XhoI
XmaI XmnI
Enzymes that do not cut:
AarI AatII AfeI AflII AhdI AleI ApaI AscI
BaeI BbvCI BcgI BmgBI BsiWI BsmBI BssHII BstEII
BstZ17I ClaI CspCI DraIII EcoRV FseI FspAI HindIII
MfeI NotI NruI PacI PfoI PmlI PshAI PspOMI
PspXI PsrI SacII SanDI SfiI SgrAI SnaBI SrfI
SwaI ZraI