Restriction map for
pF1KB7342
(Circular) (Six-base) MAPSORT of: pF1KB7342.seq Check: 4719 from: 1 to: 5795
pF1KB7342 5795 bp
With 182 enzymes: SgfI *
April 24, 2009 13:08 ..
AccI GT'mk_AC
Cuts at: 1280 2833 1280
Size: 1553 4242
Acc65I G'GTAC_C
Cuts at: 2811 2811
Size: 5795
AclI AA'CG_TT
Cuts at: 5635 5635
Size: 5795
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 214 297 869 2342 3620 4052 4799 5067 214
Size: 83 572 1473 1278 432 747 268 942
Fragments arranged by size:
1473 1278 942 747 572 432 268 83
AflII C'TTAA_G
Cuts at: 1623 1623
Size: 5795
AflIII A'CryG_T
Cuts at: 567 2236 3147 4251 5228 567
Size: 1669 911 1104 977 1134
Fragments arranged by size:
1669 1134 1104 977 911
AgeI A'CCGG_T
Cuts at: 1006 4183 1006
Size: 3177 2618
AhdI GACnn_n'nnGTC
Cuts at: 1856 1856
Size: 5795
AlfI GCAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 2793 2793
Size: 5795
AlwNI CAG_nnn'CTG
Cuts at: 3100 4667 3100
Size: 1567 4228
ApaI G_GGCC'C
Cuts at: 1513 1513
Size: 5795
ApaLI G'TGCA_C
Cuts at: 1467 4565 1467
Size: 3098 2697
ApoI r'AATT_y
Cuts at: 317 1720 2301 2755 2799 5148 5404 317
Size: 1403 581 454 44 2349 256 708
Fragments arranged by size:
2349 1403 708 581 454 256 44
AseI AT'TA_AT
Cuts at: 20 1667 20
Size: 1647 4148
AsiSI GCG_AT'CGC
Cuts at: 104 104
Size: 5795
AvaI C'yCGr_G
Cuts at: 2815 2815
Size: 5795
BaeI ACnnnnGTAyCnnnnnnn_nnnnn'
Cuts at: 995 1028 995
Size: 33 5762
BamHI G'GATC_C
Cuts at: 2820 2820
Size: 5795
BanI G'GyrC_C
Cuts at: 2811 3225 3473 3508 2811
Size: 414 248 35 5098
Fragments arranged by size:
5098 414 248 35
BanII G_rGCy'C
Cuts at: 519 1450 1513 2809 3839 519
Size: 931 63 1296 1030 2475
Fragments arranged by size:
2475 1296 1030 931 63
BbeI G_GCGC'C
Cuts at: 3229 3477 3229
Size: 248 5547
BbsI GAAGACnn'nnnn_
Cuts at: 356 2291 2645 356
Size: 1935 354 3506
Fragments arranged by size:
3506 1935 354
BcgI CGAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 712 746 712
Size: 34 5761
BciVI GTATCCnnnnn_n'
Cuts at: 1576 3693 4454 5795 1576
Size: 2117 761 1341 1576
Fragments arranged by size:
2117 1576 1341 761
BclI T'GATC_A
Cuts at: 791 3315 791
Size: 2524 3271
BfrBI ATG'CAT
Cuts at: 2780 2780
Size: 5795
BglII A'GATC_T
Cuts at: 3310 5347 3310
Size: 2037 3758
BlpI GC'TnA_GC
Cuts at: 2907 2907
Size: 5795
Bme1580I G_kGCm'C
Cuts at: 1471 1513 3420 3513 4569 1471
Size: 42 1907 93 1056 2697
Fragments arranged by size:
2697 1907 1056 93 42
BmgBI CAC'GTC
Cuts at: 1215 1215
Size: 5795
BmrI ACTGGGnnnn_n'
Cuts at: 689 3007 3088 3423 5567 689
Size: 2318 81 335 2144 917
Fragments arranged by size:
2318 2144 917 335 81
BplI GAGnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 4162 4162
Size: 5795
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 283 1511 2351 2749 283
Size: 1228 840 398 3329
Fragments arranged by size:
3329 1228 840 398
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 259 478 1159 1707 2971 3850 4342 4640 259
Size: 219 681 548 1264 879 492 298 1414
Fragments arranged by size:
1414 1264 879 681 548 492 298 219
BsaI GGTCTCn'nnnn_
Cuts at: 5499 5499
Size: 5795
BsaAI yAC'GTr
Cuts at: 3148 3148
Size: 5795
BsaBI GATnn'nnATC
Cuts at: 6 3309 5346 6
Size: 3303 2037 455
Fragments arranged by size:
3303 2037 455
BsaHI Gr'CG_yC
Cuts at: 3226 3474 4176 5672 3226
Size: 248 702 1496 3349
Fragments arranged by size:
3349 1496 702 248
BsaWI w'CCGG_w
Cuts at: 293 877 1006 2984 3505 4183 4457 4604 5015
Size: 584 129 1978 521 678 274 147 411
Cuts at: 5015 293
Size: 1073
Fragments arranged by size:
1978 1073 678 584 521 411 274 147
129
BsaXI ACnnnnnCTCCnnnnnnn_nnn'
Cuts at: 532 562 2324 2354 532
Size: 30 1762 30 3973
Fragments arranged by size:
3973 1762 30 30
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 280 1253 2635 280
Size: 973 1382 3440
Fragments arranged by size:
3440 1382 973
BseYI C'CCAG_C
Cuts at: 1560 2294 3220 3978 4555 5051 1560
Size: 734 926 758 577 496 2304
Fragments arranged by size:
2304 926 758 734 577 496
BsgI GTGCAGnnnnnnnnnnnnnn_nn'
Cuts at: 578 578
Size: 5795
BsiEI CG_ry'CG
Cuts at: 104 2709 3337 3383 4591 104
Size: 2605 628 46 1208 1308
Fragments arranged by size:
2605 1308 1208 628 46
BsiHKAI G_wGCw'C
Cuts at: 1450 1471 2809 3587 3777 4569 1450
Size: 21 1338 778 190 792 2676
Fragments arranged by size:
2676 1338 792 778 190 21
BsmI GAATG_Cn'
Cuts at: 1522 1522
Size: 5795
BsmBI CGTCTCn'nnnn_
Cuts at: 402 501 2144 402
Size: 99 1643 4053
Fragments arranged by size:
4053 1643 99
Bsp1286I G_dGCh'C
Cuts at: 519 1450 1471 1513 2809 3420 3513 3587 3777
Size: 931 21 42 1296 611 93 74 190
Cuts at: 3777 3839 4569 519
Size: 62 730 1745
Fragments arranged by size:
1745 1296 931 730 611 190 93 74
62 42 21
BspEI T'CCGG_A
Cuts at: 877 5015 877
Size: 4138 1657
BspHI T'CATG_A
Cuts at: 4971 4971
Size: 5795
BspMI ACCTGCnnnn'nnnn_
Cuts at: 2845 3059 3361 3742 2845
Size: 214 302 381 4898
Fragments arranged by size:
4898 381 302 214
BsrBI CCG'CTC
Cuts at: 50 4087 4141 5625 5790 50
Size: 4037 54 1484 165 55
Fragments arranged by size:
4037 1484 165 55 54
BsrDI GCAATG_nn'
Cuts at: 3707 3707
Size: 5795
BsrFI r'CCGG_y
Cuts at: 1006 2683 3793 3974 4183 1006
Size: 1677 1110 181 209 2618
Fragments arranged by size:
2618 1677 1110 209 181
BsrGI T'GTAC_A
Cuts at: 694 694
Size: 5795
BssSI C'ACGA_G
Cuts at: 4066 4424 4066
Size: 358 5437
BstAPI GCAn_nnn'nTGC
Cuts at: 754 1765 3172 754
Size: 1011 1407 3377
Fragments arranged by size:
3377 1407 1011
BstBI TT'CG_AA
Cuts at: 4156 4156
Size: 5795
BstXI CCAn_nnnn'nTGG
Cuts at: 114 114
Size: 5795
BstYI r'GATC_y
Cuts at: 1 578 1329 1896 2820 3287 3310 3645 3891
Size: 577 751 567 924 467 23 335 246
Cuts at: 3891 4903 4989 5001 5347 1
Size: 1012 86 12 346 449
Fragments arranged by size:
1012 924 751 577 567 467 449 346
335 246 86 23 12
BstZ17I GTA'TAC
Cuts at: 1281 1281
Size: 5795
BtgI C'CryG_G
Cuts at: 107 107
Size: 5795
BtgZI GCGATGnnnnnnnnnn'nnnn_
Cuts at: 203 3745 3926 4089 203
Size: 3542 181 163 1909
Fragments arranged by size:
3542 1909 181 163
BtsI GCAGTG_nn'
Cuts at: 1766 2249 3038 1766
Size: 483 789 4523
Fragments arranged by size:
4523 789 483
CspCI CAAnnnnnGTGGnnnnnnnnnn_nn'
Cuts at: 770 805 894 929 2472 2507 770
Size: 35 89 35 1543 35 4058
Fragments arranged by size:
4058 1543 89 35 35 35
DraI TTT'AAA
Cuts at: 2794 2794
Size: 5795
DraIII CAC_nnn'GTG
Cuts at: 843 843
Size: 5795
DrdI GACnn_nn'nnGTC
Cuts at: 2508 3501 4359 2508
Size: 993 858 3944
Fragments arranged by size:
3944 993 858
EaeI y'GGCC_r
Cuts at: 281 1566 1773 2418 2681 3380 3554 3945 3972
Size: 1285 207 645 263 699 174 391 27
Cuts at: 3972 5317 281
Size: 1345 759
Fragments arranged by size:
1345 1285 759 699 645 391 263 207
174 27
EagI C'GGCC_G
Cuts at: 3380 3380
Size: 5795
EarI CTCTTCn'nnn_
Cuts at: 463 2714 3818 4028 463
Size: 2251 1104 210 2230
Fragments arranged by size:
2251 2230 1104 210
EciI GGCGGAnnnnnnnnn_nn'
Cuts at: 4003 4313 4459 5605 4003
Size: 310 146 1146 4193
Fragments arranged by size:
4193 1146 310 146
EcoICRI GAG'CTC
Cuts at: 1448 2807 1448
Size: 1359 4436
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 214 283 297 869 1511 2342 2351 2749 3620
Size: 69 14 572 642 831 9 398 871
Cuts at: 3620 4052 4799 5067 214
Size: 432 747 268 942
Fragments arranged by size:
942 871 831 747 642 572 432 398
268 69 14 9
EcoO109I rG'GnC_Cy
Cuts at: 1509 1510 2326 2934 1509
Size: 1 816 608 4370
Fragments arranged by size:
4370 816 608 1
EcoRI G'AATT_C
Cuts at: 317 2799 317
Size: 2482 3313
EcoRV GAT'ATC
Cuts at: 687 687
Size: 5795
FalI AAGnnnnnCTTnnnnnnnn_nnnnn'
Cuts at: 226 1594 3814 226
Size: 1368 2220 2207
Fragments arranged by size:
2220 2207 1368
FspI TGC'GCA
Cuts at: 3576 3576
Size: 5795
HaeII r_GCGC'y
Cuts at: 3229 3477 4499 5478 3229
Size: 248 1022 979 3546
Fragments arranged by size:
3546 1022 979 248
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 833 865 1699 1731 1842 1874 3637 3667 3669
Size: 32 834 32 111 32 1763 30 2
Cuts at: 3669 3699 833
Size: 30 2929
Fragments arranged by size:
2929 1763 834 111 32 32 32 30
30 2
HincII GTy'rAC
Cuts at: 2005 2087 2834 2005
Size: 82 747 4966
Fragments arranged by size:
4966 747 82
HpaI GTT'AAC
Cuts at: 2087 2087
Size: 5795
KasI G'GCGC_C
Cuts at: 3225 3473 3225
Size: 248 5547
KpnI G_GTAC'C
Cuts at: 2815 2815
Size: 5795
MluI A'CGCG_T
Cuts at: 5228 5228
Size: 5795
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 2402 2504 4466 4650 2402
Size: 102 1962 184 3547
Fragments arranged by size:
3547 1962 184 102
MscI TGG'CCA
Cuts at: 1568 2420 3556 1568
Size: 852 1136 3807
Fragments arranged by size:
3807 1136 852
MslI CAynn'nnrTG
Cuts at: 1808 2121 2395 3784 3911 1808
Size: 313 274 1389 127 3692
Fragments arranged by size:
3692 1389 313 274 127
MspA1I CmG'CkG
Cuts at: 1564 2906 3220 3580 4593 4838 1564
Size: 1342 314 360 1013 245 2521
Fragments arranged by size:
2521 1342 1013 360 314 245
NaeI GCC'GGC
Cuts at: 2685 3976 2685
Size: 1291 4504
NarI GG'CG_CC
Cuts at: 3226 3474 3226
Size: 248 5547
NcoI C'CATG_G
Cuts at: 107 107
Size: 5795
NdeI CA'TA_TG
Cuts at: 2081 2081
Size: 5795
NgoMIV G'CCGG_C
Cuts at: 2683 3974 2683
Size: 1291 4504
NsiI A_TGCA'T
Cuts at: 2782 2782
Size: 5795
NspI r_CATG'y
Cuts at: 2240 2400 2780 2848 3348 4255 2240
Size: 160 380 68 500 907 3780
Fragments arranged by size:
3780 907 500 380 160 68
PasI CC'CwG_GG
Cuts at: 130 130
Size: 5795
PciI A'CATG_T
Cuts at: 2236 4251 2236
Size: 2015 3780
PmeI GTTT'AAAC
Cuts at: 2794 2794
Size: 5795
PpiI GAACnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 4934 4966 4934
Size: 32 5763
PpuMI rG'GwC_Cy
Cuts at: 2326 2326
Size: 5795
PsiI TTA'TAA
Cuts at: 2024 2024
Size: 5795
PspOMI G'GGCC_C
Cuts at: 1509 1509
Size: 5795
PstI C_TGCA'G
Cuts at: 759 2704 2842 3527 759
Size: 1945 138 685 3027
Fragments arranged by size:
3027 1945 685 138
PvuI CG_AT'CG
Cuts at: 104 2709 104
Size: 2605 3190
PvuII CAG'CTG
Cuts at: 1564 3220 3580 1564
Size: 1656 360 3779
Fragments arranged by size:
3779 1656 360
RsrII CG'GwC_CG
Cuts at: 3990 3990
Size: 5795
SacI G_AGCT'C
Cuts at: 1450 2809 1450
Size: 1359 4436
SalI G'TCGA_C
Cuts at: 2832 2832
Size: 5795
SapI GCTCTTCn'nnn_
Cuts at: 3818 4028 3818
Size: 210 5585
SbfI CC_TGCA'GG
Cuts at: 2842 2842
Size: 5795
ScaI AGT'ACT
Cuts at: 1703 5312 1703
Size: 3609 2186
SfcI C'TryA_G
Cuts at: 33 755 1892 2700 2838 3523 4516 4707 33
Size: 722 1137 808 138 685 993 191 1121
Fragments arranged by size:
1137 1121 993 808 722 685 191 138
SfoI GGC'GCC
Cuts at: 3227 3475 3227
Size: 248 5547
SgfI GCG_AT'CGC
Cuts at: 104 104
Size: 5795
SmaI CCC'GGG
Cuts at: 2817 2817
Size: 5795
SmlI C'TyrA_G
Cuts at: 274 493 1138 1623 1686 2950 3865 4357 4619
Size: 219 645 485 63 1264 915 492 262
Cuts at: 4619 274
Size: 1450
Fragments arranged by size:
1450 1264 915 645 492 485 262 219
63
SpeI A'CTAG_T
Cuts at: 67 67
Size: 5795
SphI G_CATG'C
Cuts at: 2848 3348 2848
Size: 500 5295
SspI AAT'ATT
Cuts at: 1746 2355 1746
Size: 609 5186
StyI C'CwwG_G
Cuts at: 107 1578 2504 2929 3283 107
Size: 1471 926 425 354 2619
Fragments arranged by size:
2619 1471 926 425 354
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 1013 1078 2590 3378 3511 3970 4179 1013
Size: 65 1512 788 133 459 209 2629
Fragments arranged by size:
2629 1512 788 459 209 133 65
TatI w'GTAC_w
Cuts at: 435 694 1701 2143 5310 435
Size: 259 1007 442 3167 920
Fragments arranged by size:
3167 1007 920 442 259
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 872 904 5312 5344 872
Size: 32 4408 32 1323
Fragments arranged by size:
4408 1323 32 32
Tth111I GACn'n_nGTC
Cuts at: 3592 3592
Size: 5795
XbaI T'CTAG_A
Cuts at: 321 2736 2826 321
Size: 2415 90 3290
Fragments arranged by size:
3290 2415 90
XcmI CCAnnnn_n'nnnnTGG
Cuts at: 1576 2584 3290 1576
Size: 1008 706 4081
Fragments arranged by size:
4081 1008 706
XmaI C'CCGG_G
Cuts at: 2815 2815
Size: 5795
Enzymes that do cut:
AccI Acc65I AclI AcuI AflII AflIII AgeI AhdI
AlfI AlwNI ApaI ApaLI ApoI AseI AsiSI AvaI
BaeI BamHI BanI BanII BbeI BbsI BcgI BciVI
BclI BfrBI BglII BlpI Bme1580I BmgBI BmrI BplI
BpmI BpuEI BsaI BsaAI BsaBI BsaHI BsaWI BsaXI
BseRI BseYI BsgI BsiEI BsiHKAI BsmI BsmBI Bsp1286I
BspEI BspHI BspMI BsrBI BsrDI BsrFI BsrGI BssSI
BstAPI BstBI BstXI BstYI BstZ17I BtgI BtgZI BtsI
CspCI DraI DraIII DrdI EaeI EagI EarI EciI
EcoICRI Eco57MI EcoO109I EcoRI EcoRV FalI FspI HaeII
Hin4I HincII HpaI KasI KpnI MluI MmeI MscI
MslI MspA1I NaeI NarI NcoI NdeI NgoMIV NsiI
NspI PasI PciI PmeI PpiI PpuMI PsiI PspOMI
PstI PvuI PvuII RsrII SacI SalI SapI SbfI
ScaI SfcI SfoI SgfI SmaI SmlI SpeI SphI
SspI StyI TaqII TatI TstI Tth111I XbaI XcmI
XmaI
Enzymes that do not cut:
AarI AatII AfeI AleI AloI AscI AvrII BbvCI
BglI BmtI Bpu10I BsiWI BssHII BstEII Bsu36I ClaI
EcoNI FseI FspAI HindIII MfeI NheI NotI NruI
PacI PflMI PfoI PmlI PshAI PspXI PsrI SacII
SanDI SexAI SfiI SgrAI SnaBI SrfI StuI SwaI
XhoI XmnI ZraI