Restriction map for
pF1KB7339
(Circular) (Six-base) MAPSORT of: pF1KB7339.seq Check: 9484 from: 1 to: 5612
pF1KB7339 5612 bp
With 182 enzymes: SgfI *
April 24, 2009 13:08 ..
AarI CACCTGCnnnn'nnnn_
Cuts at: 1655 2040 2232 2412 1655
Size: 385 192 180 4855
Fragments arranged by size:
4855 385 192 180
AatII G_ACGT'C
Cuts at: 116 1996 116
Size: 1880 3732
AccI GT'mk_AC
Cuts at: 2650 2650
Size: 5612
Acc65I G'GTAC_C
Cuts at: 2015 2628 2015
Size: 613 4999
AclI AA'CG_TT
Cuts at: 1757 5452 1757
Size: 3695 1917
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 262 268 691 838 1000 1993 2380 3437 3869
Size: 6 423 147 162 993 387 1057 432
Cuts at: 3869 4616 4884 262
Size: 747 268 990
Fragments arranged by size:
1057 993 990 747 432 423 387 268
162 147 6
AflIII A'CryG_T
Cuts at: 2964 4068 5045 2964
Size: 1104 977 3531
Fragments arranged by size:
3531 1104 977
AgeI A'CCGG_T
Cuts at: 4000 4000
Size: 5612
AhdI GACnn_n'nnGTC
Cuts at: 219 702 878 219
Size: 483 176 4953
Fragments arranged by size:
4953 483 176
AloI GAACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 1477 1509 1882 1914 1477
Size: 32 373 32 5175
Fragments arranged by size:
5175 373 32 32
AlwNI CAG_nnn'CTG
Cuts at: 174 480 1647 2055 2110 2460 2917 4484 174
Size: 306 1167 408 55 350 457 1567 1302
Fragments arranged by size:
1567 1302 1167 457 408 350 306 55
ApaI G_GGCC'C
Cuts at: 1403 1969 1403
Size: 566 5046
ApaLI G'TGCA_C
Cuts at: 760 4382 760
Size: 3622 1990
ApoI r'AATT_y
Cuts at: 382 2032 2616 4965 5221 382
Size: 1650 584 2349 256 773
Fragments arranged by size:
2349 1650 773 584 256
AseI AT'TA_AT
Cuts at: 20 20
Size: 5612
AsiSI GCG_AT'CGC
Cuts at: 104 104
Size: 5612
AvaI C'yCGr_G
Cuts at: 146 209 988 1030 1190 1371 1496 2143 2582
Size: 63 779 42 160 181 125 647 439
Cuts at: 2582 2632 146
Size: 50 3126
Fragments arranged by size:
3126 779 647 439 181 160 125 63
50 42
BamHI G'GATC_C
Cuts at: 1091 1598 2637 1091
Size: 507 1039 4066
Fragments arranged by size:
4066 1039 507
BanI G'GyrC_C
Cuts at: 2015 2628 3042 3290 3325 2015
Size: 613 414 248 35 4302
Fragments arranged by size:
4302 613 414 248 35
BanII G_rGCy'C
Cuts at: 443 1031 1372 1403 1960 1969 2143 2433 2626
Size: 588 341 31 557 9 174 290 193
Cuts at: 2626 3656 443
Size: 1030 2399
Fragments arranged by size:
2399 1030 588 557 341 290 193 174
31 9
BbeI G_GCGC'C
Cuts at: 3046 3294 3046
Size: 248 5364
BbvCI CC'TCA_GC
Cuts at: 2574 2574
Size: 5612
BciVI GTATCCnnnnn_n'
Cuts at: 673 939 3510 4271 5612 673
Size: 266 2571 761 1341 673
Fragments arranged by size:
2571 1341 761 673 266
BclI T'GATC_A
Cuts at: 3132 3132
Size: 5612
BglII A'GATC_T
Cuts at: 3127 5164 3127
Size: 2037 3575
BlpI GC'TnA_GC
Cuts at: 1366 2724 1366
Size: 1358 4254
Bme1580I G_kGCm'C
Cuts at: 764 1403 1563 1969 3237 3330 4386 764
Size: 639 160 406 1268 93 1056 1990
Fragments arranged by size:
1990 1268 1056 639 406 160 93
BmgBI CAC'GTC
Cuts at: 1257 1257
Size: 5612
BmrI ACTGGGnnnn_n'
Cuts at: 2314 2824 2905 3240 5384 2314
Size: 510 81 335 2144 2542
Fragments arranged by size:
2542 2144 510 335 81
BplI GAGnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 3979 3979
Size: 5612
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 388 427 457 1062 2527 388
Size: 39 30 605 1465 3473
Fragments arranged by size:
3473 1465 605 39 30
Bpu10I CC'TnA_GC
Cuts at: 2574 2574
Size: 5612
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 2788 3667 4159 4457 2788
Size: 879 492 298 3943
Fragments arranged by size:
3943 879 492 298
BsaI GGTCTCn'nnnn_
Cuts at: 134 1144 1187 1825 2459 5316 134
Size: 1010 43 638 634 2857 430
Fragments arranged by size:
2857 1010 638 634 430 43
BsaAI yAC'GTr
Cuts at: 188 1736 2965 188
Size: 1548 1229 2835
Fragments arranged by size:
2835 1548 1229
BsaBI GATnn'nnATC
Cuts at: 6 3126 5163 6
Size: 3120 2037 455
Fragments arranged by size:
3120 2037 455
BsaHI Gr'CG_yC
Cuts at: 113 612 1993 2071 3043 3291 3993 5489 113
Size: 499 1381 78 972 248 702 1496 236
Fragments arranged by size:
1496 1381 972 702 499 248 236 78
BsaWI w'CCGG_w
Cuts at: 648 1385 1595 1685 2801 3322 4000 4274 4421
Size: 737 210 90 1116 521 678 274 147
Cuts at: 4421 4832 648
Size: 411 1428
Fragments arranged by size:
1428 1116 737 678 521 411 274 210
147 90
BsaXI ACnnnnnCTCCnnnnnnn_nnn'
Cuts at: 1275 1305 1477 1507 2408 2438 1275
Size: 30 172 30 901 30 4449
Fragments arranged by size:
4449 901 172 30 30 30
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 1039 1178 1280 2533 2536 1039
Size: 139 102 1253 3 4115
Fragments arranged by size:
4115 1253 139 102 3
BseYI C'CCAG_C
Cuts at: 174 550 895 932 1161 2205 2590 3037 3795
Size: 376 345 37 229 1044 385 447 758
Cuts at: 3795 4372 4868 174
Size: 577 496 918
Fragments arranged by size:
1044 918 758 577 496 447 385 376
345 229 37
BsgI GTGCAGnnnnnnnnnnnnnn_nn'
Cuts at: 2260 2440 2532 2260
Size: 180 92 5340
Fragments arranged by size:
5340 180 92
BsiEI CG_ry'CG
Cuts at: 104 1613 2152 3154 3200 4408 104
Size: 1509 539 1002 46 1208 1308
Fragments arranged by size:
1509 1308 1208 1002 539 46
BsiHKAI G_wGCw'C
Cuts at: 443 473 764 1960 2168 2221 2228 2626 3404
Size: 30 291 1196 208 53 7 398 778
Cuts at: 3404 3594 4386 443
Size: 190 792 1669
Fragments arranged by size:
1669 1196 792 778 398 291 208 190
53 30 7
BsmBI CGTCTCn'nnnn_
Cuts at: 1984 1984
Size: 5612
Bsp1286I G_dGCh'C
Cuts at: 443 473 764 1031 1372 1403 1563 1960 1969
Size: 30 291 267 341 31 160 397 9
Cuts at: 1969 2143 2168 2221 2228 2433 2626 3237 3330
Size: 174 25 53 7 205 193 611 93
Cuts at: 3330 3404 3594 3656 4386 443
Size: 74 190 62 730 1669
Fragments arranged by size:
1669 730 611 397 341 291 267 205
193 190 174 160 93 74 62 53
31 30 25 9 7
BspEI T'CCGG_A
Cuts at: 648 1595 4832 648
Size: 947 3237 1428
Fragments arranged by size:
3237 1428 947
BspHI T'CATG_A
Cuts at: 785 4788 785
Size: 4003 1609
BspMI ACCTGCnnnn'nnnn_
Cuts at: 170 1655 2040 2232 2412 2662 2876 3178 3559
Size: 1485 385 192 180 250 214 302 381
Cuts at: 3559 170
Size: 2223
Fragments arranged by size:
2223 1485 385 381 302 250 214 192
180
BsrBI CCG'CTC
Cuts at: 50 750 1076 1174 3904 3958 5442 5607 50
Size: 700 326 98 2730 54 1484 165 55
Fragments arranged by size:
2730 1484 700 326 165 98 55 54
BsrDI GCAATG_nn'
Cuts at: 3524 3524
Size: 5612
BsrFI r'CCGG_y
Cuts at: 314 3610 3791 4000 314
Size: 3296 181 209 1926
Fragments arranged by size:
3296 1926 209 181
BssHII G'CGCG_C
Cuts at: 2189 2189
Size: 5612
BssSI C'ACGA_G
Cuts at: 3883 4241 3883
Size: 358 5254
BstAPI GCAn_nnn'nTGC
Cuts at: 2418 2989 2418
Size: 571 5041
BstBI TT'CG_AA
Cuts at: 3973 3973
Size: 5612
BstEII G'GTnAC_C
Cuts at: 2244 2301 2244
Size: 57 5555
BstXI CCAn_nnnn'nTGG
Cuts at: 330 729 1086 330
Size: 399 357 4856
Fragments arranged by size:
4856 399 357
BstYI r'GATC_y
Cuts at: 1 371 963 1063 1091 1598 1907 2345 2637
Size: 370 592 100 28 507 309 438 292
Cuts at: 2637 3104 3127 3462 3708 4720 4806 4818 5164
Size: 467 23 335 246 1012 86 12 346
Cuts at: 5164 1
Size: 449
Fragments arranged by size:
1012 592 507 467 449 438 370 346
335 309 292 246 100 86 28 23
12
Bsu36I CC'TnA_GG
Cuts at: 1198 1198
Size: 5612
BtgI C'CryG_G
Cuts at: 107 339 2372 107
Size: 232 2033 3347
Fragments arranged by size:
3347 2033 232
BtgZI GCGATGnnnnnnnnnn'nnnn_
Cuts at: 1836 3562 3743 3906 1836
Size: 1726 181 163 3542
Fragments arranged by size:
3542 1726 181 163
BtsI GCAGTG_nn'
Cuts at: 2062 2520 2532 2855 2062
Size: 458 12 323 4819
Fragments arranged by size:
4819 458 323 12
DraI TTT'AAA
Cuts at: 2611 2611
Size: 5612
DraIII CAC_nnn'GTG
Cuts at: 2178 2178
Size: 5612
DrdI GACnn_nn'nnGTC
Cuts at: 2076 3318 4176 2076
Size: 1242 858 3512
Fragments arranged by size:
3512 1242 858
EaeI y'GGCC_r
Cuts at: 605 719 731 1610 2149 3197 3371 3762 3789
Size: 114 12 879 539 1048 174 391 27
Cuts at: 3789 5134 605
Size: 1345 1083
Fragments arranged by size:
1345 1083 1048 879 539 391 174 114
27 12
EagI C'GGCC_G
Cuts at: 1610 2149 3197 1610
Size: 539 1048 4025
Fragments arranged by size:
4025 1048 539
EarI CTCTTCn'nnn_
Cuts at: 784 1540 2173 3635 3845 784
Size: 756 633 1462 210 2551
Fragments arranged by size:
2551 1462 756 633 210
EciI GGCGGAnnnnnnnnn_nn'
Cuts at: 1071 1960 3820 4130 4276 5422 1071
Size: 889 1860 310 146 1146 1261
Fragments arranged by size:
1860 1261 1146 889 310 146
EcoICRI GAG'CTC
Cuts at: 441 1958 2624 441
Size: 1517 666 3429
Fragments arranged by size:
3429 1517 666
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 262 268 388 427 457 691 838 1000 1062
Size: 6 120 39 30 234 147 162 62
Cuts at: 1062 1993 2380 2527 3437 3869 4616 4884 262
Size: 931 387 147 910 432 747 268 990
Fragments arranged by size:
990 931 910 747 432 387 268 234
162 147 147 120 62 39 30 6
EcoNI CCTnn'n_nnAGG
Cuts at: 286 286
Size: 5612
EcoO109I rG'GnC_Cy
Cuts at: 228 997 1336 1400 1965 2233 2315 2751 228
Size: 769 339 64 565 268 82 436 3089
Fragments arranged by size:
3089 769 565 436 339 268 82 64
EcoRI G'AATT_C
Cuts at: 382 2616 382
Size: 2234 3378
FalI AAGnnnnnCTTnnnnnnnn_nnnnn'
Cuts at: 3631 3631
Size: 5612
FspI TGC'GCA
Cuts at: 3393 3393
Size: 5612
HaeII r_GCGC'y
Cuts at: 902 3046 3294 4316 5295 902
Size: 2144 248 1022 979 1219
Fragments arranged by size:
2144 1219 1022 979 248
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 205 237 259 291 518 550 688 720 864
Size: 32 22 32 227 32 138 32 144
Cuts at: 864 896 900 932 941 948 973 980 2514
Size: 32 4 32 9 7 25 7 1534
Cuts at: 2514 2546 3454 3484 3486 3516 205
Size: 32 908 30 2 30 2301
Fragments arranged by size:
2301 1534 908 227 144 138 32 32
32 32 32 32 32 30 30 25
22 9 7 7 4 2
HincII GTy'rAC
Cuts at: 2651 2651
Size: 5612
KasI G'GCGC_C
Cuts at: 3042 3290 3042
Size: 248 5364
KpnI G_GTAC'C
Cuts at: 2019 2632 2019
Size: 613 4999
MluI A'CGCG_T
Cuts at: 5045 5045
Size: 5612
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 2364 4283 4467 2364
Size: 1919 184 3509
Fragments arranged by size:
3509 1919 184
MscI TGG'CCA
Cuts at: 607 3373 607
Size: 2766 2846
MslI CAynn'nnrTG
Cuts at: 907 2134 3601 3728 907
Size: 1227 1467 127 2791
Fragments arranged by size:
2791 1467 1227 127
MspA1I CmG'CkG
Cuts at: 171 661 1365 2122 2448 2723 3037 3397 4410
Size: 490 704 757 326 275 314 360 1013
Cuts at: 4410 4655 171
Size: 245 1128
Fragments arranged by size:
1128 1013 757 704 490 360 326 314
275 245
NaeI GCC'GGC
Cuts at: 316 3793 316
Size: 3477 2135
NarI GG'CG_CC
Cuts at: 3043 3291 3043
Size: 248 5364
NcoI C'CATG_G
Cuts at: 107 339 107
Size: 232 5380
NgoMIV G'CCGG_C
Cuts at: 314 3791 314
Size: 3477 2135
NspI r_CATG'y
Cuts at: 2665 3165 4072 2665
Size: 500 907 4205
Fragments arranged by size:
4205 907 500
PasI CC'CwG_GG
Cuts at: 708 708
Size: 5612
PciI A'CATG_T
Cuts at: 4068 4068
Size: 5612
PflMI CCAn_nnn'nTGG
Cuts at: 480 480
Size: 5612
PfoI T'CCnGG_A
Cuts at: 404 959 1910 404
Size: 555 951 4106
Fragments arranged by size:
4106 951 555
PmeI GTTT'AAAC
Cuts at: 2611 2611
Size: 5612
PpiI GAACnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 1463 1477 1495 1509 4751 4783 1463
Size: 14 18 14 3242 32 2292
Fragments arranged by size:
3242 2292 32 18 14 14
PpuMI rG'GwC_Cy
Cuts at: 228 997 1336 2233 2315 228
Size: 769 339 897 82 3525
Fragments arranged by size:
3525 897 769 339 82
PshAI GACnn'nnGTC
Cuts at: 1109 1334 1109
Size: 225 5387
PspOMI G'GGCC_C
Cuts at: 1399 1965 1399
Size: 566 5046
PstI C_TGCA'G
Cuts at: 1278 2659 3344 1278
Size: 1381 685 3546
Fragments arranged by size:
3546 1381 685
PvuI CG_AT'CG
Cuts at: 104 104
Size: 5612
PvuII CAG'CTG
Cuts at: 661 1365 2448 3037 3397 661
Size: 704 1083 589 360 2876
Fragments arranged by size:
2876 1083 704 589 360
RsrII CG'GwC_CG
Cuts at: 3807 3807
Size: 5612
SacI G_AGCT'C
Cuts at: 443 1960 2626 443
Size: 1517 666 3429
Fragments arranged by size:
3429 1517 666
SalI G'TCGA_C
Cuts at: 2649 2649
Size: 5612
SanDI GG'GwC_CC
Cuts at: 2233 2315 2233
Size: 82 5530
SapI GCTCTTCn'nnn_
Cuts at: 2173 3635 3845 2173
Size: 1462 210 3940
Fragments arranged by size:
3940 1462 210
SbfI CC_TGCA'GG
Cuts at: 2659 2659
Size: 5612
ScaI AGT'ACT
Cuts at: 5129 5129
Size: 5612
SexAI A'CCwGG_T
Cuts at: 230 230
Size: 5612
SfcI C'TryA_G
Cuts at: 33 166 739 1180 1274 2452 2655 3340 4333
Size: 133 573 441 94 1178 203 685 993
Cuts at: 4333 4524 33
Size: 191 1121
Fragments arranged by size:
1178 1121 993 685 573 441 203 191
133 94
SfoI GGC'GCC
Cuts at: 3044 3292 3044
Size: 248 5364
SgfI GCG_AT'CGC
Cuts at: 104 104
Size: 5612
SmaI CCC'GGG
Cuts at: 148 990 1373 1498 2145 2634 148
Size: 842 383 125 647 489 3126
Fragments arranged by size:
3126 842 647 489 383 125
SmlI C'TyrA_G
Cuts at: 209 1190 2767 3682 4174 4436 209
Size: 981 1577 915 492 262 1385
Fragments arranged by size:
1577 1385 981 915 492 262
SpeI A'CTAG_T
Cuts at: 67 67
Size: 5612
SphI G_CATG'C
Cuts at: 2665 3165 2665
Size: 500 5112
StuI AGG'CCT
Cuts at: 953 2401 2603 953
Size: 1448 202 3962
Fragments arranged by size:
3962 1448 202
StyI C'CwwG_G
Cuts at: 107 339 1634 1809 2746 3100 107
Size: 232 1295 175 937 354 2619
Fragments arranged by size:
2619 1295 937 354 232 175
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 166 1084 1728 2371 3195 3328 3787 3996 166
Size: 918 644 643 824 133 459 209 1782
Fragments arranged by size:
1782 918 824 644 643 459 209 133
TatI w'GTAC_w
Cuts at: 1742 5127 1742
Size: 3385 2227
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 1275 1307 2330 2362 2408 2440 5129 5161 1275
Size: 32 1023 32 46 32 2689 32 1726
Fragments arranged by size:
2689 1726 1023 46 32 32 32 32
Tth111I GACn'n_nGTC
Cuts at: 3409 3409
Size: 5612
XbaI T'CTAG_A
Cuts at: 2643 2643
Size: 5612
XcmI CCAnnnn_n'nnnnTGG
Cuts at: 114 3107 114
Size: 2993 2619
XhoI C'TCGA_G
Cuts at: 209 1190 209
Size: 981 4631
XmaI C'CCGG_G
Cuts at: 146 988 1371 1496 2143 2632 146
Size: 842 383 125 647 489 3126
Fragments arranged by size:
3126 842 647 489 383 125
XmnI GAAnn'nnTTC
Cuts at: 2032 2032
Size: 5612
ZraI GAC'GTC
Cuts at: 114 1994 114
Size: 1880 3732
Enzymes that do cut:
AarI AatII AccI Acc65I AclI AcuI AflIII AgeI
AhdI AloI AlwNI ApaI ApaLI ApoI AseI AsiSI
AvaI BamHI BanI BanII BbeI BbvCI BciVI BclI
BglII BlpI Bme1580I BmgBI BmrI BplI BpmI Bpu10I
BpuEI BsaI BsaAI BsaBI BsaHI BsaWI BsaXI BseRI
BseYI BsgI BsiEI BsiHKAI BsmBI Bsp1286I BspEI BspHI
BspMI BsrBI BsrDI BsrFI BssHII BssSI BstAPI BstBI
BstEII BstXI BstYI Bsu36I BtgI BtgZI BtsI DraI
DraIII DrdI EaeI EagI EarI EciI EcoICRI Eco57MI
EcoNI EcoO109I EcoRI FalI FspI HaeII Hin4I HincII
KasI KpnI MluI MmeI MscI MslI MspA1I NaeI
NarI NcoI NgoMIV NspI PasI PciI PflMI PfoI
PmeI PpiI PpuMI PshAI PspOMI PstI PvuI PvuII
RsrII SacI SalI SanDI SapI SbfI ScaI SexAI
SfcI SfoI SgfI SmaI SmlI SpeI SphI StuI
StyI TaqII TatI TstI Tth111I XbaI XcmI XhoI
XmaI XmnI ZraI
Enzymes that do not cut:
AfeI AflII AleI AlfI AscI AvrII BaeI BbsI
BcgI BfrBI BglI BmtI BsiWI BsmI BsrGI BstZ17I
ClaI CspCI EcoRV FseI FspAI HindIII HpaI MfeI
NdeI NheI NotI NruI NsiI PacI PmlI PsiI
PspXI PsrI SacII SfiI SgrAI SnaBI SrfI SspI
SwaI