Restriction map for
pF1KB7337
(Circular) (Six-base) MAPSORT of: pF1KB7337.seq Check: 981 from: 1 to: 6242
pF1KB7337 6242 bp
With 182 enzymes: SgfI *
April 24, 2009 13:08 ..
AarI CACCTGCnnnn'nnnn_
Cuts at: 136 136
Size: 6242
AccI GT'mk_AC
Cuts at: 3211 3280 3211
Size: 69 6173
Acc65I G'GTAC_C
Cuts at: 142 1112 3258 142
Size: 970 2146 3126
Fragments arranged by size:
3126 2146 970
AclI AA'CG_TT
Cuts at: 6082 6082
Size: 6242
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 1432 2425 2457 2983 4067 4499 5246 5514 1432
Size: 993 32 526 1084 432 747 268 2160
Fragments arranged by size:
2160 1084 993 747 526 432 268 32
AflII C'TTAA_G
Cuts at: 1219 1219
Size: 6242
AflIII A'CryG_T
Cuts at: 1821 2622 3594 4698 5675 1821
Size: 801 972 1104 977 2388
Fragments arranged by size:
2388 1104 977 972 801
AgeI A'CCGG_T
Cuts at: 4630 4630
Size: 6242
AhdI GACnn_n'nnGTC
Cuts at: 240 240
Size: 6242
AlfI GCAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 2772 2772
Size: 6242
AloI GAACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 1386 1418 1386
Size: 32 6210
AlwNI CAG_nnn'CTG
Cuts at: 228 2385 2892 3547 5114 228
Size: 2157 507 655 1567 1356
Fragments arranged by size:
2157 1567 1356 655 507
ApaI G_GGCC'C
Cuts at: 1554 1554
Size: 6242
ApaLI G'TGCA_C
Cuts at: 5012 5012
Size: 6242
ApoI r'AATT_y
Cuts at: 199 1771 2210 2895 3246 5595 5851 199
Size: 1572 439 685 351 2349 256 590
Fragments arranged by size:
2349 1572 685 590 439 351 256
AseI AT'TA_AT
Cuts at: 20 20
Size: 6242
AsiSI GCG_AT'CGC
Cuts at: 104 104
Size: 6242
AvaI C'yCGr_G
Cuts at: 3232 3262 3232
Size: 30 6212
BaeI ACnnnnGTAyCnnnnnnn_nnnnn'
Cuts at: 126 159 126
Size: 33 6209
BamHI G'GATC_C
Cuts at: 765 3267 765
Size: 2502 3740
BanI G'GyrC_C
Cuts at: 142 1112 3258 3672 3920 3955 142
Size: 970 2146 414 248 35 2429
Fragments arranged by size:
2429 2146 970 414 248 35
BanII G_rGCy'C
Cuts at: 786 1124 1554 2729 3256 4286 786
Size: 338 430 1175 527 1030 2742
Fragments arranged by size:
2742 1175 1030 527 430 338
BbeI G_GCGC'C
Cuts at: 3676 3924 3676
Size: 248 5994
BbsI GAAGACnn'nnnn_
Cuts at: 835 1616 2057 835
Size: 781 441 5020
Fragments arranged by size:
5020 781 441
BbvCI CC'TCA_GC
Cuts at: 2950 2950
Size: 6242
BcgI CGAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 967 1001 967
Size: 34 6208
BciVI GTATCCnnnnn_n'
Cuts at: 280 3162 4140 4901 6242 280
Size: 2882 978 761 1341 280
Fragments arranged by size:
2882 1341 978 761 280
BclI T'GATC_A
Cuts at: 1187 3762 1187
Size: 2575 3667
BglII A'GATC_T
Cuts at: 468 1215 3757 5794 468
Size: 747 2542 2037 916
Fragments arranged by size:
2542 2037 916 747
BlpI GC'TnA_GC
Cuts at: 3354 3354
Size: 6242
Bme1580I G_kGCm'C
Cuts at: 260 1380 1554 3867 3960 5016 260
Size: 1120 174 2313 93 1056 1486
Fragments arranged by size:
2313 1486 1120 1056 174 93
BmgBI CAC'GTC
Cuts at: 1824 2390 1824
Size: 566 5676
BmrI ACTGGGnnnn_n'
Cuts at: 870 1120 3454 3535 3870 6014 870
Size: 250 2334 81 335 2144 1098
Fragments arranged by size:
2334 2144 1098 335 250 81
BplI GAGnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 1089 4609 1089
Size: 3520 2722
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 214 694 1426 1838 2236 2578 2743 214
Size: 480 732 412 398 342 165 3713
Fragments arranged by size:
3713 732 480 412 398 342 165
Bpu10I CC'TnA_GC
Cuts at: 2167 2950 2167
Size: 783 5459
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 306 1296 2527 3418 4297 4789 5087 306
Size: 990 1231 891 879 492 298 1461
Fragments arranged by size:
1461 1231 990 891 879 492 298
BsaI GGTCTCn'nnnn_
Cuts at: 2347 5946 2347
Size: 3599 2643
BsaAI yAC'GTr
Cuts at: 711 2625 3595 711
Size: 1914 970 3358
Fragments arranged by size:
3358 1914 970
BsaBI GATnn'nnATC
Cuts at: 6 185 1192 3756 5793 6
Size: 179 1007 2564 2037 455
Fragments arranged by size:
2564 2037 1007 455 179
BsaHI Gr'CG_yC
Cuts at: 3673 3921 4623 6119 3673
Size: 248 702 1496 3796
Fragments arranged by size:
3796 1496 702 248
BsaWI w'CCGG_w
Cuts at: 3431 3952 4630 4904 5051 5462 3431
Size: 521 678 274 147 411 4211
Fragments arranged by size:
4211 678 521 411 274 147
BsaXI ACnnnnnCTCCnnnnnnn_nnn'
Cuts at: 1483 1513 2716 2746 1483
Size: 30 1203 30 4979
Fragments arranged by size:
4979 1203 30 30
BseYI C'CCAG_C
Cuts at: 221 2487 3667 4425 5002 5498 221
Size: 2266 1180 758 577 496 965
Fragments arranged by size:
2266 1180 965 758 577 496
BsgI GTGCAGnnnnnnnnnnnnnn_nn'
Cuts at: 454 841 454
Size: 387 5855
BsiEI CG_ry'CG
Cuts at: 104 3784 3830 5038 104
Size: 3680 46 1208 1308
Fragments arranged by size:
3680 1308 1208 46
BsiHKAI G_wGCw'C
Cuts at: 1124 2729 3256 4034 4224 5016 1124
Size: 1605 527 778 190 792 2350
Fragments arranged by size:
2350 1605 792 778 527 190
BsmBI CGTCTCn'nnnn_
Cuts at: 2825 2825
Size: 6242
Bsp1286I G_dGCh'C
Cuts at: 260 786 1124 1380 1554 2729 3256 3867 3960
Size: 526 338 256 174 1175 527 611 93
Cuts at: 3960 4034 4224 4286 5016 260
Size: 74 190 62 730 1486
Fragments arranged by size:
1486 1175 730 611 527 526 338 256
190 174 93 74 62
BspEI T'CCGG_A
Cuts at: 5462 5462
Size: 6242
BspHI T'CATG_A
Cuts at: 1292 5418 1292
Size: 4126 2116
BspMI ACCTGCnnnn'nnnn_
Cuts at: 136 2891 3292 3506 3808 4189 136
Size: 2755 401 214 302 381 2189
Fragments arranged by size:
2755 2189 401 381 302 214
BsrBI CCG'CTC
Cuts at: 50 4534 4588 6072 6237 50
Size: 4484 54 1484 165 55
Fragments arranged by size:
4484 1484 165 55 54
BsrDI GCAATG_nn'
Cuts at: 848 3049 3165 4154 848
Size: 2201 116 989 2936
Fragments arranged by size:
2936 2201 989 116
BsrFI r'CCGG_y
Cuts at: 4240 4421 4630 4240
Size: 181 209 5852
Fragments arranged by size:
5852 209 181
BsrGI T'GTAC_A
Cuts at: 570 1102 1248 570
Size: 532 146 5564
Fragments arranged by size:
5564 532 146
BssSI C'ACGA_G
Cuts at: 2035 4513 4871 2035
Size: 2478 358 3406
Fragments arranged by size:
3406 2478 358
BstAPI GCAn_nnn'nTGC
Cuts at: 852 3619 852
Size: 2767 3475
BstBI TT'CG_AA
Cuts at: 4603 4603
Size: 6242
BstEII G'GTnAC_C
Cuts at: 2878 2878
Size: 6242
BstXI CCAn_nnnn'nTGG
Cuts at: 1544 1944 2665 1544
Size: 400 721 5121
Fragments arranged by size:
5121 721 400
BstYI r'GATC_y
Cuts at: 1 453 468 522 591 765 1215 3267 3734
Size: 452 15 54 69 174 450 2052 467
Cuts at: 3734 3757 4092 4338 5350 5436 5448 5794 1
Size: 23 335 246 1012 86 12 346 449
Fragments arranged by size:
2052 1012 467 452 450 449 346 335
246 174 86 69 54 23 15 12
BtgI C'CryG_G
Cuts at: 1382 1382
Size: 6242
BtgZI GCGATGnnnnnnnnnn'nnnn_
Cuts at: 4192 4373 4536 4192
Size: 181 163 5898
Fragments arranged by size:
5898 181 163
BtsI GCAGTG_nn'
Cuts at: 1380 2025 3485 1380
Size: 645 1460 4137
Fragments arranged by size:
4137 1460 645
CspCI CAAnnnnnGTGGnnnnnnnnnn_nn'
Cuts at: 1671 1706 1671
Size: 35 6207
DraI TTT'AAA
Cuts at: 1883 3241 1883
Size: 1358 4884
DraIII CAC_nnn'GTG
Cuts at: 400 3046 400
Size: 2646 3596
DrdI GACnn_nn'nnGTC
Cuts at: 2839 3948 4806 2839
Size: 1109 858 4275
Fragments arranged by size:
4275 1109 858
EaeI y'GGCC_r
Cuts at: 1534 1565 3827 4001 4392 4419 5764 1534
Size: 31 2262 174 391 27 1345 2012
Fragments arranged by size:
2262 2012 1345 391 174 31 27
EagI C'GGCC_G
Cuts at: 3827 3827
Size: 6242
EarI CTCTTCn'nnn_
Cuts at: 992 1964 3112 4265 4475 992
Size: 972 1148 1153 210 2759
Fragments arranged by size:
2759 1153 1148 972 210
EciI GGCGGAnnnnnnnnn_nn'
Cuts at: 4450 4760 4906 6052 4450
Size: 310 146 1146 4640
Fragments arranged by size:
4640 1146 310 146
EcoICRI GAG'CTC
Cuts at: 1122 2727 3254 1122
Size: 1605 527 4110
Fragments arranged by size:
4110 1605 527
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 214 694 1426 1432 1838 2236 2425 2457 2578
Size: 480 732 6 406 398 189 32 121
Cuts at: 2578 2743 2983 4067 4499 5246 5514 214
Size: 165 240 1084 432 747 268 942
Fragments arranged by size:
1084 942 747 732 480 432 406 398
268 240 189 165 121 32 6
EcoNI CCTnn'n_nnAGG
Cuts at: 2854 2854
Size: 6242
EcoO109I rG'GnC_Cy
Cuts at: 1550 1551 1626 2424 2592 3381 1550
Size: 1 75 798 168 789 4411
Fragments arranged by size:
4411 798 789 168 75 1
EcoRI G'AATT_C
Cuts at: 199 1771 3246 199
Size: 1572 1475 3195
Fragments arranged by size:
3195 1572 1475
FalI AAGnnnnnCTTnnnnnnnn_nnnnn'
Cuts at: 228 852 2110 2727 4261 228
Size: 624 1258 617 1534 2209
Fragments arranged by size:
2209 1534 1258 624 617
FspI TGC'GCA
Cuts at: 4023 4023
Size: 6242
HaeII r_GCGC'y
Cuts at: 759 3676 3924 4946 5925 759
Size: 2917 248 1022 979 1076
Fragments arranged by size:
2917 1076 1022 979 248
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 226 258 369 401 991 1023 1078 1110 1429
Size: 32 111 32 590 32 55 32 319
Cuts at: 1429 1461 2058 2090 2360 2392 2711 2743 4084
Size: 32 597 32 270 32 319 32 1341
Cuts at: 4084 4114 4116 4146 226
Size: 30 2 30 2322
Fragments arranged by size:
2322 1341 597 590 319 319 270 111
55 32 32 32 32 32 32 32
32 30 30 2
HincII GTy'rAC
Cuts at: 2091 3212 3281 2091
Size: 1121 69 5052
Fragments arranged by size:
5052 1121 69
HindIII A'AGCT_T
Cuts at: 205 2123 2516 205
Size: 1918 393 3931
Fragments arranged by size:
3931 1918 393
KasI G'GCGC_C
Cuts at: 3672 3920 3672
Size: 248 5994
KpnI G_GTAC'C
Cuts at: 146 1116 3262 146
Size: 970 2146 3126
Fragments arranged by size:
3126 2146 970
MluI A'CGCG_T
Cuts at: 5675 5675
Size: 6242
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 481 1372 1514 2643 4913 5097 481
Size: 891 142 1129 2270 184 1626
Fragments arranged by size:
2270 1626 1129 891 184 142
MscI TGG'CCA
Cuts at: 1536 1567 4003 1536
Size: 31 2436 3775
Fragments arranged by size:
3775 2436 31
MslI CAynn'nnrTG
Cuts at: 1304 4231 4358 1304
Size: 2927 127 3188
Fragments arranged by size:
3188 2927 127
MspA1I CmG'CkG
Cuts at: 192 228 914 1532 2585 2742 3353 3667 4027
Size: 36 686 618 1053 157 611 314 360
Cuts at: 4027 5040 5285 192
Size: 1013 245 1149
Fragments arranged by size:
1149 1053 1013 686 618 611 360 314
245 157 36
NaeI GCC'GGC
Cuts at: 4423 4423
Size: 6242
NarI GG'CG_CC
Cuts at: 3673 3921 3673
Size: 248 5994
NcoI C'CATG_G
Cuts at: 1382 1382
Size: 6242
NgoMIV G'CCGG_C
Cuts at: 4421 4421
Size: 6242
NspI r_CATG'y
Cuts at: 3218 3295 3795 4702 3218
Size: 77 500 907 4758
Fragments arranged by size:
4758 907 500 77
PasI CC'CwG_GG
Cuts at: 252 275 510 579 1937 252
Size: 23 235 69 1358 4557
Fragments arranged by size:
4557 1358 235 69 23
PciI A'CATG_T
Cuts at: 4698 4698
Size: 6242
PfoI T'CCnGG_A
Cuts at: 1724 2236 1724
Size: 512 5730
PmeI GTTT'AAAC
Cuts at: 3241 3241
Size: 6242
PpiI GAACnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 2461 2493 5381 5413 2461
Size: 32 2888 32 3290
Fragments arranged by size:
3290 2888 32 32
PpuMI rG'GwC_Cy
Cuts at: 2424 2592 2424
Size: 168 6074
PshAI GACnn'nnGTC
Cuts at: 799 799
Size: 6242
PspOMI G'GGCC_C
Cuts at: 1550 1550
Size: 6242
PstI C_TGCA'G
Cuts at: 755 1337 1375 1561 2423 2786 2888 3227 3289
Size: 582 38 186 862 363 102 339 62
Cuts at: 3289 3974 755
Size: 685 3023
Fragments arranged by size:
3023 862 685 582 363 339 186 102
62 38
PvuI CG_AT'CG
Cuts at: 104 104
Size: 6242
PvuII CAG'CTG
Cuts at: 192 228 914 1532 2585 2742 3667 4027 192
Size: 36 686 618 1053 157 925 360 2407
Fragments arranged by size:
2407 1053 925 686 618 360 157 36
RsrII CG'GwC_CG
Cuts at: 4437 4437
Size: 6242
SacI G_AGCT'C
Cuts at: 1124 2729 3256 1124
Size: 1605 527 4110
Fragments arranged by size:
4110 1605 527
SalI G'TCGA_C
Cuts at: 3210 3279 3210
Size: 69 6173
SapI GCTCTTCn'nnn_
Cuts at: 4265 4475 4265
Size: 210 6032
SbfI CC_TGCA'GG
Cuts at: 3289 3289
Size: 6242
ScaI AGT'ACT
Cuts at: 5759 5759
Size: 6242
SexAI A'CCwGG_T
Cuts at: 1201 1201
Size: 6242
SfcI C'TryA_G
Cuts at: 33 751 1333 1371 1557 2419 2782 2884 3223
Size: 718 582 38 186 862 363 102 339
Cuts at: 3223 3285 3970 4963 5154 33
Size: 62 685 993 191 1121
Fragments arranged by size:
1121 993 862 718 685 582 363 339
191 186 102 62 38
SfoI GGC'GCC
Cuts at: 3674 3922 3674
Size: 248 5994
SgfI GCG_AT'CGC
Cuts at: 104 104
Size: 6242
SmaI CCC'GGG
Cuts at: 3264 3264
Size: 6242
SmlI C'TyrA_G
Cuts at: 285 1219 1311 2542 3397 4312 4804 5066 285
Size: 934 92 1231 855 915 492 262 1461
Fragments arranged by size:
1461 1231 934 915 855 492 262 92
SpeI A'CTAG_T
Cuts at: 67 67
Size: 6242
SphI G_CATG'C
Cuts at: 3295 3795 3295
Size: 500 5742
SspI AAT'ATT
Cuts at: 1668 2100 1668
Size: 432 5810
StuI AGG'CCT
Cuts at: 1225 2860 1225
Size: 1635 4607
StyI C'CwwG_G
Cuts at: 150 836 1382 2861 3376 3730 150
Size: 686 546 1479 515 354 2662
Fragments arranged by size:
2662 1479 686 546 515 354
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 1726 3825 3958 4417 4626 1726
Size: 2099 133 459 209 3342
Fragments arranged by size:
3342 2099 459 209 133
TatI w'GTAC_w
Cuts at: 570 1102 1248 5757 570
Size: 532 146 4509 1055
Fragments arranged by size:
4509 1055 532 146
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 5759 5791 5759
Size: 32 6210
Tth111I GACn'n_nGTC
Cuts at: 4039 4039
Size: 6242
XbaI T'CTAG_A
Cuts at: 471 3273 471
Size: 2802 3440
XcmI CCAnnnn_n'nnnnTGG
Cuts at: 1106 3737 1106
Size: 2631 3611
XmaI C'CCGG_G
Cuts at: 3262 3262
Size: 6242
XmnI GAAnn'nnTTC
Cuts at: 1166 1166
Size: 6242
Enzymes that do cut:
AarI AccI Acc65I AclI AcuI AflII AflIII AgeI
AhdI AlfI AloI AlwNI ApaI ApaLI ApoI AseI
AsiSI AvaI BaeI BamHI BanI BanII BbeI BbsI
BbvCI BcgI BciVI BclI BglII BlpI Bme1580I BmgBI
BmrI BplI BpmI Bpu10I BpuEI BsaI BsaAI BsaBI
BsaHI BsaWI BsaXI BseYI BsgI BsiEI BsiHKAI BsmBI
Bsp1286I BspEI BspHI BspMI BsrBI BsrDI BsrFI BsrGI
BssSI BstAPI BstBI BstEII BstXI BstYI BtgI BtgZI
BtsI CspCI DraI DraIII DrdI EaeI EagI EarI
EciI EcoICRI Eco57MI EcoNI EcoO109I EcoRI FalI FspI
HaeII Hin4I HincII HindIII KasI KpnI MluI MmeI
MscI MslI MspA1I NaeI NarI NcoI NgoMIV NspI
PasI PciI PfoI PmeI PpiI PpuMI PshAI PspOMI
PstI PvuI PvuII RsrII SacI SalI SapI SbfI
ScaI SexAI SfcI SfoI SgfI SmaI SmlI SpeI
SphI SspI StuI StyI TaqII TatI TstI Tth111I
XbaI XcmI XmaI XmnI
Enzymes that do not cut:
AatII AfeI AleI AscI AvrII BfrBI BglI BmtI
BseRI BsiWI BsmI BssHII BstZ17I Bsu36I ClaI EcoRV
FseI FspAI HpaI MfeI NdeI NheI NotI NruI
NsiI PacI PflMI PmlI PsiI PspXI PsrI SacII
SanDI SfiI SgrAI SnaBI SrfI SwaI XhoI ZraI