Restriction map for
pF1KB7318
(Circular) (Six-base) MAPSORT of: pF1KB7318.seq Check: 1798 from: 1 to: 6656
pF1KB7318 6656 bp
With 182 enzymes: SgfI *
April 24, 2009 13:07 ..
AarI CACCTGCnnnn'nnnn_
Cuts at: 2405 2485 2405
Size: 80 6576
AccI GT'mk_AC
Cuts at: 3694 3694
Size: 6656
Acc65I G'GTAC_C
Cuts at: 3672 3672
Size: 6656
AclI AA'CG_TT
Cuts at: 844 3650 6496 844
Size: 2806 2846 1004
Fragments arranged by size:
2846 2806 1004
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 356 590 1654 2617 2722 3271 3337 3435 4481
Size: 234 1064 963 105 549 66 98 1046
Cuts at: 4481 4913 5660 5928 356
Size: 432 747 268 1084
Fragments arranged by size:
1084 1064 1046 963 747 549 432 268
234 105 98 66
AfeI AGC'GCT
Cuts at: 1639 1639
Size: 6656
AflIII A'CryG_T
Cuts at: 1838 4008 5112 6089 1838
Size: 2170 1104 977 2405
Fragments arranged by size:
2405 2170 1104 977
AgeI A'CCGG_T
Cuts at: 869 3632 5044 869
Size: 2763 1412 2481
Fragments arranged by size:
2763 2481 1412
AleI CACnn'nnGTG
Cuts at: 2916 2916
Size: 6656
AlfI GCAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 2096 2096
Size: 6656
AloI GAACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 1346 1378 1346
Size: 32 6624
AlwNI CAG_nnn'CTG
Cuts at: 1775 2202 2493 2832 2877 3003 3174 3961 5528
Size: 427 291 339 45 126 171 787 1567
Cuts at: 5528 1775
Size: 2903
Fragments arranged by size:
2903 1567 787 427 339 291 171 126
45
ApaLI G'TGCA_C
Cuts at: 5426 5426
Size: 6656
ApoI r'AATT_y
Cuts at: 334 617 674 775 2255 3660 6009 6265 334
Size: 283 57 101 1480 1405 2349 256 725
Fragments arranged by size:
2349 1480 1405 725 283 256 101 57
AseI AT'TA_AT
Cuts at: 20 20
Size: 6656
AsiSI GCG_AT'CGC
Cuts at: 104 104
Size: 6656
AvaI C'yCGr_G
Cuts at: 1586 1717 3234 3346 3587 3676 1586
Size: 131 1517 112 241 89 4566
Fragments arranged by size:
4566 1517 241 131 112 89
BamHI G'GATC_C
Cuts at: 3681 3681
Size: 6656
BanI G'GyrC_C
Cuts at: 700 1458 2383 2587 3672 4086 4334 4369 700
Size: 758 925 204 1085 414 248 35 2987
Fragments arranged by size:
2987 1085 925 758 414 248 204 35
BanII G_rGCy'C
Cuts at: 244 1156 1474 1733 3670 4700 244
Size: 912 318 259 1937 1030 2200
Fragments arranged by size:
2200 1937 1030 912 318 259
BbeI G_GCGC'C
Cuts at: 4090 4338 4090
Size: 248 6408
BbsI GAAGACnn'nnnn_
Cuts at: 990 1408 1897 1985 2452 3119 990
Size: 418 489 88 467 667 4527
Fragments arranged by size:
4527 667 489 467 418 88
BbvCI CC'TCA_GC
Cuts at: 782 1234 2300 2464 2883 782
Size: 452 1066 164 419 4555
Fragments arranged by size:
4555 1066 452 419 164
BciVI GTATCCnnnnn_n'
Cuts at: 4554 5315 6656 4554
Size: 761 1341 4554
Fragments arranged by size:
4554 1341 761
BclI T'GATC_A
Cuts at: 4176 4176
Size: 6656
BglII A'GATC_T
Cuts at: 2705 4171 6208 2705
Size: 1466 2037 3153
Fragments arranged by size:
3153 2037 1466
BlpI GC'TnA_GC
Cuts at: 3768 3768
Size: 6656
Bme1580I G_kGCm'C
Cuts at: 1291 1463 4281 4374 5430 1291
Size: 172 2818 93 1056 2517
Fragments arranged by size:
2818 2517 1056 172 93
BmgBI CAC'GTC
Cuts at: 1284 1506 3500 1284
Size: 222 1994 4440
Fragments arranged by size:
4440 1994 222
BmrI ACTGGGnnnn_n'
Cuts at: 1200 2061 2808 3868 3949 4284 6428 1200
Size: 861 747 1060 81 335 2144 1428
Fragments arranged by size:
2144 1428 1060 861 747 335 81
BplI GAGnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 1074 3408 5023 1074
Size: 2334 1615 2707
Fragments arranged by size:
2707 2334 1615
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 1044 1636 1795 2017 2215 2559 2860 3151 3196
Size: 592 159 222 198 344 301 291 45
Cuts at: 3196 3583 1044
Size: 387 4117
Fragments arranged by size:
4117 592 387 344 301 291 222 198
159 45
Bpu10I CC'TnA_GC
Cuts at: 238 782 1234 2300 2464 2883 3088 3094 238
Size: 544 452 1066 164 419 205 6 3800
Fragments arranged by size:
3800 1066 544 452 419 205 164 6
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 1285 1396 2117 2823 3565 3832 4711 5203 5501
Size: 111 721 706 742 267 879 492 298
Cuts at: 5501 1285
Size: 2440
Fragments arranged by size:
2440 879 742 721 706 492 298 267
111
BsaI GGTCTCn'nnnn_
Cuts at: 2061 6360 2061
Size: 4299 2357
BsaAI yAC'GTr
Cuts at: 166 765 1839 4009 166
Size: 599 1074 2170 2813
Fragments arranged by size:
2813 2170 1074 599
BsaBI GATnn'nnATC
Cuts at: 6 4170 6207 6
Size: 4164 2037 455
Fragments arranged by size:
4164 2037 455
BsaHI Gr'CG_yC
Cuts at: 2108 4087 4335 5037 6533 2108
Size: 1979 248 702 1496 2231
Fragments arranged by size:
2231 1979 1496 702 248
BsaWI w'CCGG_w
Cuts at: 869 1086 2900 3632 3845 4366 5044 5318 5465
Size: 217 1814 732 213 521 678 274 147
Cuts at: 5465 5876 869
Size: 411 1649
Fragments arranged by size:
1814 1649 732 678 521 411 274 217
213 147
BsaXI ACnnnnnCTCCnnnnnnn_nnn'
Cuts at: 1087 1117 1651 1681 1087
Size: 30 534 30 6062
Fragments arranged by size:
6062 534 30 30
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 430 799 802 1795 2365 3252 430
Size: 369 3 993 570 887 3834
Fragments arranged by size:
3834 993 887 570 369 3
BseYI C'CCAG_C
Cuts at: 956 2007 2731 4081 4839 5416 5912 956
Size: 1051 724 1350 758 577 496 1700
Fragments arranged by size:
1700 1350 1051 758 724 577 496
BsgI GTGCAGnnnnnnnnnnnnnn_nn'
Cuts at: 886 1537 2032 2548 3460 3592 886
Size: 651 495 516 912 132 3950
Fragments arranged by size:
3950 912 651 516 495 132
BsiEI CG_ry'CG
Cuts at: 104 4198 4244 5452 104
Size: 4094 46 1208 1308
Fragments arranged by size:
4094 1308 1208 46
BsiHKAI G_wGCw'C
Cuts at: 171 244 1733 1740 3670 4448 4638 5430 171
Size: 73 1489 7 1930 778 190 792 1397
Fragments arranged by size:
1930 1489 1397 792 778 190 73 7
BsmI GAATG_Cn'
Cuts at: 420 3612 420
Size: 3192 3464
BsmBI CGTCTCn'nnnn_
Cuts at: 1274 1274
Size: 6656
Bsp1286I G_dGCh'C
Cuts at: 171 244 1156 1291 1463 1474 1733 1740 3670
Size: 73 912 135 172 11 259 7 1930
Cuts at: 3670 4281 4374 4448 4638 4700 5430 171
Size: 611 93 74 190 62 730 1397
Fragments arranged by size:
1930 1397 912 730 611 259 190 172
135 93 74 73 62 11 7
BspEI T'CCGG_A
Cuts at: 5876 5876
Size: 6656
BspHI T'CATG_A
Cuts at: 1424 3143 5832 1424
Size: 1719 2689 2248
Fragments arranged by size:
2689 2248 1719
BspMI ACCTGCnnnn'nnnn_
Cuts at: 518 2405 2485 3706 3920 4222 4603 518
Size: 1887 80 1221 214 302 381 2571
Fragments arranged by size:
2571 1887 1221 381 302 214 80
BsrBI CCG'CTC
Cuts at: 50 2436 4948 5002 6486 6651 50
Size: 2386 2512 54 1484 165 55
Fragments arranged by size:
2512 2386 1484 165 55 54
BsrDI GCAATG_nn'
Cuts at: 310 4568 310
Size: 4258 2398
BsrFI r'CCGG_y
Cuts at: 869 3413 3632 4654 4835 5044 869
Size: 2544 219 1022 181 209 2481
Fragments arranged by size:
2544 2481 1022 219 209 181
BssSI C'ACGA_G
Cuts at: 1969 4927 5285 1969
Size: 2958 358 3340
Fragments arranged by size:
3340 2958 358
BstAPI GCAn_nnn'nTGC
Cuts at: 4033 4033
Size: 6656
BstBI TT'CG_AA
Cuts at: 5017 5017
Size: 6656
BstYI r'GATC_y
Cuts at: 1 564 2276 2569 2705 3261 3681 4148 4171
Size: 563 1712 293 136 556 420 467 23
Cuts at: 4171 4506 4752 5764 5850 5862 6208 1
Size: 335 246 1012 86 12 346 449
Fragments arranged by size:
1712 1012 563 556 467 449 420 346
335 293 246 136 86 23 12
BtgI C'CryG_G
Cuts at: 1466 1904 2861 1466
Size: 438 957 5261
Fragments arranged by size:
5261 957 438
BtgZI GCGATGnnnnnnnnnn'nnnn_
Cuts at: 449 4606 4787 4950 449
Size: 4157 181 163 2155
Fragments arranged by size:
4157 2155 181 163
BtsI GCAGTG_nn'
Cuts at: 815 1047 2362 3899 815
Size: 232 1315 1537 3572
Fragments arranged by size:
3572 1537 1315 232
CspCI CAAnnnnnGTGGnnnnnnnnnn_nn'
Cuts at: 1924 1959 2161 2196 1924
Size: 35 202 35 6384
Fragments arranged by size:
6384 202 35 35
DraI TTT'AAA
Cuts at: 3655 3655
Size: 6656
DraIII CAC_nnn'GTG
Cuts at: 166 166
Size: 6656
DrdI GACnn_nn'nnGTC
Cuts at: 1899 4362 5220 1899
Size: 2463 858 3335
Fragments arranged by size:
3335 2463 858
EaeI y'GGCC_r
Cuts at: 829 1069 2552 4241 4415 4806 4833 6178 829
Size: 240 1483 1689 174 391 27 1345 1307
Fragments arranged by size:
1689 1483 1345 1307 391 240 174 27
EagI C'GGCC_G
Cuts at: 4241 4241
Size: 6656
EarI CTCTTCn'nnn_
Cuts at: 2597 3247 4679 4889 2597
Size: 650 1432 210 4364
Fragments arranged by size:
4364 1432 650 210
EciI GGCGGAnnnnnnnnn_nn'
Cuts at: 4864 5174 5320 6466 4864
Size: 310 146 1146 5054
Fragments arranged by size:
5054 1146 310 146
EcoICRI GAG'CTC
Cuts at: 242 1731 3668 242
Size: 1489 1937 3230
Fragments arranged by size:
3230 1937 1489
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 356 590 1044 1636 1654 1795 2017 2215 2559
Size: 234 454 592 18 141 222 198 344
Cuts at: 2559 2617 2722 2860 3151 3196 3271 3337 3435
Size: 58 105 138 291 45 75 66 98
Cuts at: 3435 3583 4481 4913 5660 5928 356
Size: 148 898 432 747 268 1084
Fragments arranged by size:
1084 898 747 592 454 432 344 291
268 234 222 198 148 141 138 105
98 75 66 58 45 18
EcoNI CCTnn'n_nnAGG
Cuts at: 2347 2347
Size: 6656
EcoO109I rG'GnC_Cy
Cuts at: 190 1242 1848 2001 3171 3795 190
Size: 1052 606 153 1170 624 3051
Fragments arranged by size:
3051 1170 1052 624 606 153
EcoRI G'AATT_C
Cuts at: 3660 3660
Size: 6656
FalI AAGnnnnnCTTnnnnnnnn_nnnnn'
Cuts at: 1974 2200 4675 1974
Size: 226 2475 3955
Fragments arranged by size:
3955 2475 226
FspI TGC'GCA
Cuts at: 4437 4437
Size: 6656
HaeII r_GCGC'y
Cuts at: 1605 1641 4090 4338 5360 6339 1605
Size: 36 2449 248 1022 979 1922
Fragments arranged by size:
2449 1922 1022 979 248 36
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 1087 1119 1138 1170 1300 1332 1580 1612 2356
Size: 32 19 32 130 32 248 32 744
Cuts at: 2356 2388 2997 3029 3205 3237 3246 3278 4498
Size: 32 609 32 176 32 9 32 1220
Cuts at: 4498 4528 4530 4560 1087
Size: 30 2 30 3183
Fragments arranged by size:
3183 1220 744 609 248 176 130 32
32 32 32 32 32 32 32 30
30 19 9 2
HincII GTy'rAC
Cuts at: 3695 3695
Size: 6656
HindIII A'AGCT_T
Cuts at: 2041 3127 2041
Size: 1086 5570
KasI G'GCGC_C
Cuts at: 4086 4334 4086
Size: 248 6408
KpnI G_GTAC'C
Cuts at: 3676 3676
Size: 6656
MluI A'CGCG_T
Cuts at: 6089 6089
Size: 6656
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 2633 2951 5327 5511 2633
Size: 318 2376 184 3778
Fragments arranged by size:
3778 2376 318 184
MscI TGG'CCA
Cuts at: 1071 2554 4417 1071
Size: 1483 1863 3310
Fragments arranged by size:
3310 1863 1483
MslI CAynn'nnrTG
Cuts at: 737 1483 2916 4645 4772 737
Size: 746 1433 1729 127 2621
Fragments arranged by size:
2621 1729 1433 746 127
MspA1I CmG'CkG
Cuts at: 652 1026 1497 2299 2406 3767 4081 4441 5454
Size: 374 471 802 107 1361 314 360 1013
Cuts at: 5454 5699 652
Size: 245 1609
Fragments arranged by size:
1609 1361 1013 802 471 374 360 314
245 107
NaeI GCC'GGC
Cuts at: 4837 4837
Size: 6656
NarI GG'CG_CC
Cuts at: 4087 4335 4087
Size: 248 6408
NcoI C'CATG_G
Cuts at: 1904 1904
Size: 6656
NgoMIV G'CCGG_C
Cuts at: 4835 4835
Size: 6656
NspI r_CATG'y
Cuts at: 389 2309 3362 3709 4209 5116 389
Size: 1920 1053 347 500 907 1929
Fragments arranged by size:
1929 1920 1053 907 500 347
PasI CC'CwG_GG
Cuts at: 2025 3279 2025
Size: 1254 5402
PciI A'CATG_T
Cuts at: 5112 5112
Size: 6656
PflMI CCAn_nnn'nTGG
Cuts at: 1067 2202 2877 3174 1067
Size: 1135 675 297 4549
Fragments arranged by size:
4549 1135 675 297
PfoI T'CCnGG_A
Cuts at: 1811 3167 1811
Size: 1356 5300
PmeI GTTT'AAAC
Cuts at: 3655 3655
Size: 6656
PmlI CAC'GTG
Cuts at: 166 765 166
Size: 599 6057
PpiI GAACnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 5795 5827 5795
Size: 32 6624
PpuMI rG'GwC_Cy
Cuts at: 190 1242 2001 3171 190
Size: 1052 759 1170 3675
Fragments arranged by size:
3675 1170 1052 759
PspXI vC'TCGA_Gb
Cuts at: 1586 1586
Size: 6656
PstI C_TGCA'G
Cuts at: 657 1496 1502 2828 2999 3703 4388 657
Size: 839 6 1326 171 704 685 2925
Fragments arranged by size:
2925 1326 839 704 685 171 6
PvuI CG_AT'CG
Cuts at: 104 104
Size: 6656
PvuII CAG'CTG
Cuts at: 652 1497 2406 4081 4441 652
Size: 845 909 1675 360 2867
Fragments arranged by size:
2867 1675 909 845 360
RsrII CG'GwC_CG
Cuts at: 4851 4851
Size: 6656
SacI G_AGCT'C
Cuts at: 244 1733 3670 244
Size: 1489 1937 3230
Fragments arranged by size:
3230 1937 1489
SalI G'TCGA_C
Cuts at: 3693 3693
Size: 6656
SapI GCTCTTCn'nnn_
Cuts at: 3247 4679 4889 3247
Size: 1432 210 5014
Fragments arranged by size:
5014 1432 210
SbfI CC_TGCA'GG
Cuts at: 3703 3703
Size: 6656
ScaI AGT'ACT
Cuts at: 1786 6173 1786
Size: 4387 2269
SexAI A'CCwGG_T
Cuts at: 3434 3434
Size: 6656
SfcI C'TryA_G
Cuts at: 33 653 1492 1498 2824 2988 2995 3699 4384
Size: 620 839 6 1326 164 7 704 685
Cuts at: 4384 5377 5568 33
Size: 993 191 1121
Fragments arranged by size:
1326 1121 993 839 704 685 620 191
164 7 6
SfoI GGC'GCC
Cuts at: 4088 4336 4088
Size: 248 6408
SgfI GCG_AT'CGC
Cuts at: 104 104
Size: 6656
SmaI CCC'GGG
Cuts at: 3678 3678
Size: 6656
SmlI C'TyrA_G
Cuts at: 1300 1375 1586 2132 2802 3234 3580 3811 4726
Size: 75 211 546 670 432 346 231 915
Cuts at: 4726 5218 5480 1300
Size: 492 262 2476
Fragments arranged by size:
2476 915 670 546 492 432 346 262
231 211 75
SpeI A'CTAG_T
Cuts at: 67 67
Size: 6656
SphI G_CATG'C
Cuts at: 3709 4209 3709
Size: 500 6156
StuI AGG'CCT
Cuts at: 1550 1550
Size: 6656
StyI C'CwwG_G
Cuts at: 588 1904 3395 3790 4144 588
Size: 1316 1491 395 354 3100
Fragments arranged by size:
3100 1491 1316 395 354
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 2401 2405 2605 2654 3271 4239 4372 4831 5040
Size: 4 200 49 617 968 133 459 209
Cuts at: 5040 2401
Size: 4017
Fragments arranged by size:
4017 968 617 459 209 200 133 49
4
TatI w'GTAC_w
Cuts at: 1784 1832 6171 1784
Size: 48 4339 2269
Fragments arranged by size:
4339 2269 48
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 1651 1683 1985 2017 2607 2639 2750 2782 2934
Size: 32 302 32 590 32 111 32 152
Cuts at: 2934 2966 6173 6205 1651
Size: 32 3207 32 2102
Fragments arranged by size:
3207 2102 590 302 152 111 32 32
32 32 32 32
Tth111I GACn'n_nGTC
Cuts at: 4453 4453
Size: 6656
XbaI T'CTAG_A
Cuts at: 3687 3687
Size: 6656
XcmI CCAnnnn_n'nnnnTGG
Cuts at: 2934 3432 4151 2934
Size: 498 719 5439
Fragments arranged by size:
5439 719 498
XhoI C'TCGA_G
Cuts at: 1586 3234 1586
Size: 1648 5008
XmaI C'CCGG_G
Cuts at: 3676 3676
Size: 6656
XmnI GAAnn'nnTTC
Cuts at: 335 1639 1954 3448 335
Size: 1304 315 1494 3543
Fragments arranged by size:
3543 1494 1304 315
Enzymes that do cut:
AarI AccI Acc65I AclI AcuI AfeI AflIII AgeI
AleI AlfI AloI AlwNI ApaLI ApoI AseI AsiSI
AvaI BamHI BanI BanII BbeI BbsI BbvCI BciVI
BclI BglII BlpI Bme1580I BmgBI BmrI BplI BpmI
Bpu10I BpuEI BsaI BsaAI BsaBI BsaHI BsaWI BsaXI
BseRI BseYI BsgI BsiEI BsiHKAI BsmI BsmBI Bsp1286I
BspEI BspHI BspMI BsrBI BsrDI BsrFI BssSI BstAPI
BstBI BstYI BtgI BtgZI BtsI CspCI DraI DraIII
DrdI EaeI EagI EarI EciI EcoICRI Eco57MI EcoNI
EcoO109I EcoRI FalI FspI HaeII Hin4I HincII HindIII
KasI KpnI MluI MmeI MscI MslI MspA1I NaeI
NarI NcoI NgoMIV NspI PasI PciI PflMI PfoI
PmeI PmlI PpiI PpuMI PspXI PstI PvuI PvuII
RsrII SacI SalI SapI SbfI ScaI SexAI SfcI
SfoI SgfI SmaI SmlI SpeI SphI StuI StyI
TaqII TatI TstI Tth111I XbaI XcmI XhoI XmaI
XmnI
Enzymes that do not cut:
AatII AflII AhdI ApaI AscI AvrII BaeI BcgI
BfrBI BglI BmtI BsiWI BsrGI BssHII BstEII BstXI
BstZ17I Bsu36I ClaI EcoRV FseI FspAI HpaI MfeI
NdeI NheI NotI NruI NsiI PacI PshAI PsiI
PspOMI PsrI SacII SanDI SfiI SgrAI SnaBI SrfI
SspI SwaI ZraI