Restriction map for
pF1KB7317
(Circular) (Six-base) MAPSORT of: pF1KB7317.seq Check: 8552 from: 1 to: 6128
pF1KB7317 6128 bp
With 182 enzymes: SgfI *
April 24, 2009 13:07 ..
AarI CACCTGCnnnn'nnnn_
Cuts at: 184 466 2825 184
Size: 282 2359 3487
Fragments arranged by size:
3487 2359 282
AccI GT'mk_AC
Cuts at: 220 3166 220
Size: 2946 3182
Acc65I G'GTAC_C
Cuts at: 2669 2945 3144 2669
Size: 276 199 5653
Fragments arranged by size:
5653 276 199
AclI AA'CG_TT
Cuts at: 5968 5968
Size: 6128
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 1659 3953 4385 5132 5400 1659
Size: 2294 432 747 268 2387
Fragments arranged by size:
2387 2294 747 432 268
AflIII A'CryG_T
Cuts at: 215 311 1631 2567 3480 4584 5561 215
Size: 96 1320 936 913 1104 977 782
Fragments arranged by size:
1320 1104 977 936 913 782 96
AgeI A'CCGG_T
Cuts at: 4516 4516
Size: 6128
AhdI GACnn_n'nnGTC
Cuts at: 1068 1068
Size: 6128
AleI CACnn'nnGTG
Cuts at: 178 613 178
Size: 435 5693
AlfI GCAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 454 454
Size: 6128
AloI GAACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 1099 1131 1099
Size: 32 6096
AlwNI CAG_nnn'CTG
Cuts at: 961 3433 5000 961
Size: 2472 1567 2089
Fragments arranged by size:
2472 2089 1567
ApaI G_GGCC'C
Cuts at: 1155 3087 3110 1155
Size: 1932 23 4173
Fragments arranged by size:
4173 1932 23
ApaLI G'TGCA_C
Cuts at: 4898 4898
Size: 6128
ApoI r'AATT_y
Cuts at: 204 3132 5481 5737 204
Size: 2928 2349 256 595
Fragments arranged by size:
2928 2349 595 256
AseI AT'TA_AT
Cuts at: 20 20
Size: 6128
AsiSI GCG_AT'CGC
Cuts at: 104 104
Size: 6128
AvaI C'yCGr_G
Cuts at: 119 377 1375 1495 1908 1988 2203 3148 119
Size: 258 998 120 413 80 215 945 3099
Fragments arranged by size:
3099 998 945 413 258 215 120 80
BamHI G'GATC_C
Cuts at: 2009 3153 2009
Size: 1144 4984
BanI G'GyrC_C
Cuts at: 385 454 574 673 1098 1252 1621 1872 2073
Size: 69 120 99 425 154 369 251 201
Cuts at: 2073 2341 2669 2837 2945 3116 3144 3558 3806
Size: 268 328 168 108 171 28 414 248
Cuts at: 3806 3841 385
Size: 35 2672
Fragments arranged by size:
2672 425 414 369 328 268 251 248
201 171 168 154 120 108 99 69
35 28
BanII G_rGCy'C
Cuts at: 340 1155 1375 1382 1496 1650 1928 2450 3087
Size: 815 220 7 114 154 278 522 637
Cuts at: 3087 3110 3142 4172 340
Size: 23 32 1030 2296
Fragments arranged by size:
2296 1030 815 637 522 278 220 154
114 32 23 7
BbeI G_GCGC'C
Cuts at: 389 3562 3810 389
Size: 3173 248 2707
Fragments arranged by size:
3173 2707 248
BbsI GAAGACnn'nnnn_
Cuts at: 1878 1878
Size: 6128
BbvCI CC'TCA_GC
Cuts at: 634 1039 1074 1656 634
Size: 405 35 582 5106
Fragments arranged by size:
5106 582 405 35
BcgI CGAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 532 566 2458 2492 2737 2771 532
Size: 34 1892 34 245 34 3889
Fragments arranged by size:
3889 1892 245 34 34 34
BciVI GTATCCnnnnn_n'
Cuts at: 263 2965 4026 4787 6128 263
Size: 2702 1061 761 1341 263
Fragments arranged by size:
2702 1341 1061 761 263
BclI T'GATC_A
Cuts at: 3648 3648
Size: 6128
BglI GCCn_nnn'nGGC
Cuts at: 163 682 928 1973 2107 2530 3114 163
Size: 519 246 1045 134 423 584 3177
Fragments arranged by size:
3177 1045 584 519 423 246 134
BglII A'GATC_T
Cuts at: 3643 5680 3643
Size: 2037 4091
BlpI GC'TnA_GC
Cuts at: 3240 3240
Size: 6128
Bme1580I G_kGCm'C
Cuts at: 457 721 875 981 1155 1224 1268 1624 2096
Size: 264 154 106 174 69 44 356 472
Cuts at: 2096 2842 3087 3110 3753 3846 4902 457
Size: 746 245 23 643 93 1056 1683
Fragments arranged by size:
1683 1056 746 643 472 356 264 245
174 154 106 93 69 44 23
BmgBI CAC'GTC
Cuts at: 218 2568 3015 218
Size: 2350 447 3331
Fragments arranged by size:
3331 2350 447
BmrI ACTGGGnnnn_n'
Cuts at: 246 1923 3340 3421 3756 5900 246
Size: 1677 1417 81 335 2144 474
Fragments arranged by size:
2144 1677 1417 474 335 81
BplI GAGnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 2280 4495 2280
Size: 2215 3913
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 508 995 1101 1165 1540 2057 2482 508
Size: 487 106 64 375 517 425 4154
Fragments arranged by size:
4154 517 487 425 375 106 64
Bpu10I CC'TnA_GC
Cuts at: 634 1039 1074 1369 1656 2152 634
Size: 405 35 295 287 496 4610
Fragments arranged by size:
4610 496 405 295 287 35
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 583 1561 1597 3304 4183 4675 4973 583
Size: 978 36 1707 879 492 298 1738
Fragments arranged by size:
1738 1707 978 879 492 298 36
BsaI GGTCTCn'nnnn_
Cuts at: 460 1690 2378 2945 5832 460
Size: 1230 688 567 2887 756
Fragments arranged by size:
2887 1230 756 688 567
BsaAI yAC'GTr
Cuts at: 251 314 1632 3481 251
Size: 63 1318 1849 2898
Fragments arranged by size:
2898 1849 1318 63
BsaBI GATnn'nnATC
Cuts at: 6 2014 2509 3642 5679 6
Size: 2008 495 1133 2037 455
Fragments arranged by size:
2037 2008 1133 495 455
BsaHI Gr'CG_yC
Cuts at: 386 594 2161 3559 3807 4509 6005 386
Size: 208 1567 1398 248 702 1496 509
Fragments arranged by size:
1567 1496 1398 702 509 248 208
BsaWI w'CCGG_w
Cuts at: 2858 3317 3838 4516 4790 4937 5348 2858
Size: 459 521 678 274 147 411 3638
Fragments arranged by size:
3638 678 521 459 411 274 147
BsaXI ACnnnnnCTCCnnnnnnn_nnn'
Cuts at: 847 877 985 1015 1099 1129 2653 2683 847
Size: 30 108 30 84 30 1524 30 4292
Fragments arranged by size:
4292 1524 108 84 30 30 30 30
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 835 922 1858 1972 2348 2544 2677 2967 835
Size: 87 936 114 376 196 133 290 3996
Fragments arranged by size:
3996 936 376 290 196 133 114 87
BseYI C'CCAG_C
Cuts at: 368 450 939 1268 3086 3553 4311 4888 5384
Size: 82 489 329 1818 467 758 577 496
Cuts at: 5384 368
Size: 1112
Fragments arranged by size:
1818 1112 758 577 496 489 467 329
82
BsgI GTGCAGnnnnnnnnnnnnnn_nn'
Cuts at: 912 1065 1073 1306 2692 912
Size: 153 8 233 1386 4348
Fragments arranged by size:
4348 1386 233 153 8
BsiEI CG_ry'CG
Cuts at: 104 158 557 3670 3716 4924 104
Size: 54 399 3113 46 1208 1308
Fragments arranged by size:
3113 1308 1208 399 54 46
BsiHKAI G_wGCw'C
Cuts at: 3142 3920 4110 4902 3142
Size: 778 190 792 4368
Fragments arranged by size:
4368 792 778 190
BsmI GAATG_Cn'
Cuts at: 3068 3068
Size: 6128
BsmBI CGTCTCn'nnnn_
Cuts at: 374 601 1479 1608 374
Size: 227 878 129 4894
Fragments arranged by size:
4894 878 227 129
Bsp1286I G_dGCh'C
Cuts at: 340 457 721 875 981 1155 1224 1268 1375
Size: 117 264 154 106 174 69 44 107
Cuts at: 1375 1382 1496 1624 1650 1928 2096 2450 2842
Size: 7 114 128 26 278 168 354 392
Cuts at: 2842 3087 3110 3142 3753 3846 3920 4110 4172
Size: 245 23 32 611 93 74 190 62
Cuts at: 4172 4902 340
Size: 730 1566
Fragments arranged by size:
1566 730 611 392 354 278 264 245
190 174 168 154 128 117 114 107
106 93 74 69 62 44 32 26
23 7
BspEI T'CCGG_A
Cuts at: 2858 5348 2858
Size: 2490 3638
BspHI T'CATG_A
Cuts at: 332 5304 332
Size: 4972 1156
BspMI ACCTGCnnnn'nnnn_
Cuts at: 184 466 1319 2825 3178 3392 3694 4075 184
Size: 282 853 1506 353 214 302 381 2237
Fragments arranged by size:
2237 1506 853 381 353 302 282 214
BsrBI CCG'CTC
Cuts at: 50 825 912 1250 1455 2616 4420 4474 5958
Size: 775 87 338 205 1161 1804 54 1484
Cuts at: 5958 6123 50
Size: 165 55
Fragments arranged by size:
1804 1484 1161 775 338 205 165 87
55 54
BsrDI GCAATG_nn'
Cuts at: 548 2240 4040 548
Size: 1692 1800 2636
Fragments arranged by size:
2636 1800 1692
BsrFI r'CCGG_y
Cuts at: 898 2119 2344 2942 3113 4126 4307 4516 898
Size: 1221 225 598 171 1013 181 209 2510
Fragments arranged by size:
2510 1221 1013 598 225 209 181 171
BssHII G'CGCG_C
Cuts at: 190 3018 190
Size: 2828 3300
BssSI C'ACGA_G
Cuts at: 363 868 4399 4757 363
Size: 505 3531 358 1734
Fragments arranged by size:
3531 1734 505 358
BstAPI GCAn_nnn'nTGC
Cuts at: 3505 3505
Size: 6128
BstBI TT'CG_AA
Cuts at: 4489 4489
Size: 6128
BstXI CCAn_nnnn'nTGG
Cuts at: 1299 1314 1692 2937 1299
Size: 15 378 1245 4490
Fragments arranged by size:
4490 1245 378 15
BstYI r'GATC_y
Cuts at: 1 1742 2009 2504 3056 3153 3620 3643 3978
Size: 1741 267 495 552 97 467 23 335
Cuts at: 3978 4224 5236 5322 5334 5680 1
Size: 246 1012 86 12 346 449
Fragments arranged by size:
1741 1012 552 495 467 449 346 335
267 246 97 86 23 12
Bsu36I CC'TnA_GG
Cuts at: 2281 2281
Size: 6128
BtgI C'CryG_G
Cuts at: 107 158 185 228 725 1685 2096 2220 2492
Size: 51 27 43 497 960 411 124 272
Cuts at: 2492 2687 2909 107
Size: 195 222 3326
Fragments arranged by size:
3326 960 497 411 272 222 195 124
51 43 27
BtgZI GCGATGnnnnnnnnnn'nnnn_
Cuts at: 2520 4078 4259 4422 2520
Size: 1558 181 163 4226
Fragments arranged by size:
4226 1558 181 163
BtsI GCAGTG_nn'
Cuts at: 627 764 900 1185 3371 627
Size: 137 136 285 2186 3384
Fragments arranged by size:
3384 2186 285 137 136
DraI TTT'AAA
Cuts at: 3127 3127
Size: 6128
DrdI GACnn_nn'nnGTC
Cuts at: 3834 4692 3834
Size: 858 5270
EaeI y'GGCC_r
Cuts at: 155 170 554 683 1087 1173 1304 1668 2099
Size: 15 384 129 404 86 131 364 431
Cuts at: 2099 2223 2390 2558 3713 3887 4278 4305 5650
Size: 124 167 168 1155 174 391 27 1345
Cuts at: 5650 155
Size: 633
Fragments arranged by size:
1345 1155 633 431 404 391 384 364
174 168 167 131 129 124 86 27
15
EagI C'GGCC_G
Cuts at: 155 554 3713 155
Size: 399 3159 2570
Fragments arranged by size:
3159 2570 399
EarI CTCTTCn'nnn_
Cuts at: 1795 4151 4361 1795
Size: 2356 210 3562
Fragments arranged by size:
3562 2356 210
EciI GGCGGAnnnnnnnnn_nn'
Cuts at: 2188 2984 4336 4646 4792 5938 2188
Size: 796 1352 310 146 1146 2378
Fragments arranged by size:
2378 1352 1146 796 310 146
EcoICRI GAG'CTC
Cuts at: 3140 3140
Size: 6128
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 508 995 1101 1165 1540 1659 2057 2482 3953
Size: 487 106 64 375 119 398 425 1471
Cuts at: 3953 4385 5132 5400 508
Size: 432 747 268 1236
Fragments arranged by size:
1471 1236 747 487 432 425 398 375
268 119 106 64
EcoNI CCTnn'n_nnAGG
Cuts at: 1285 2155 2280 1285
Size: 870 125 5133
Fragments arranged by size:
5133 870 125
EcoO109I rG'GnC_Cy
Cuts at: 372 628 1152 2109 2285 2901 3106 3107 3267
Size: 256 524 957 176 616 205 1 160
Cuts at: 3267 372
Size: 3233
Fragments arranged by size:
3233 957 616 524 256 205 176 160
1
EcoRI G'AATT_C
Cuts at: 3132 3132
Size: 6128
FalI AAGnnnnnCTTnnnnnnnn_nnnnn'
Cuts at: 486 663 4147 486
Size: 177 3484 2467
Fragments arranged by size:
3484 2467 177
FspI TGC'GCA
Cuts at: 361 619 733 3909 361
Size: 258 114 3176 2580
Fragments arranged by size:
3176 2580 258 114
HaeII r_GCGC'y
Cuts at: 389 3562 3810 4832 5811 389
Size: 3173 248 1022 979 706
Fragments arranged by size:
3173 1022 979 706 248
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 763 795 1054 1086 1586 1618 1842 1874 3970
Size: 32 259 32 500 32 224 32 2096
Cuts at: 3970 4000 4002 4032 763
Size: 30 2 30 2859
Fragments arranged by size:
2859 2096 500 259 224 32 32 32
32 30 30 2
HincII GTy'rAC
Cuts at: 221 1401 2412 3167 221
Size: 1180 1011 755 3182
Fragments arranged by size:
3182 1180 1011 755
KasI G'GCGC_C
Cuts at: 385 3558 3806 385
Size: 3173 248 2707
Fragments arranged by size:
3173 2707 248
KpnI G_GTAC'C
Cuts at: 2673 2949 3148 2673
Size: 276 199 5653
Fragments arranged by size:
5653 276 199
MluI A'CGCG_T
Cuts at: 5561 5561
Size: 6128
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 473 1388 4799 4983 473
Size: 915 3411 184 1618
Fragments arranged by size:
3411 1618 915 184
MscI TGG'CCA
Cuts at: 685 1306 2101 3889 685
Size: 621 795 1788 2924
Fragments arranged by size:
2924 1788 795 621
MslI CAynn'nnrTG
Cuts at: 178 613 1690 2308 2935 3037 4117 4244 178
Size: 435 1077 618 627 102 1080 127 2062
Fragments arranged by size:
2062 1080 1077 627 618 435 127 102
MspA1I CmG'CkG
Cuts at: 160 187 972 983 1211 1232 1393 2085 2316
Size: 27 785 11 228 21 161 692 231
Cuts at: 2316 2516 2751 2888 3090 3239 3553 3913 4926
Size: 200 235 137 202 149 314 360 1013
Cuts at: 4926 5171 160
Size: 245 1117
Fragments arranged by size:
1117 1013 785 692 360 314 245 235
231 228 202 200 161 149 137 27
21 11
NaeI GCC'GGC
Cuts at: 900 2121 2346 3115 4309 900
Size: 1221 225 769 1194 2719
Fragments arranged by size:
2719 1221 1194 769 225
NarI GG'CG_CC
Cuts at: 386 3559 3807 386
Size: 3173 248 2707
Fragments arranged by size:
3173 2707 248
NcoI C'CATG_G
Cuts at: 107 725 1685 2687 107
Size: 618 960 1002 3548
Fragments arranged by size:
3548 1002 960 618
NgoMIV G'CCGG_C
Cuts at: 898 2119 2344 3113 4307 898
Size: 1221 225 769 1194 2719
Fragments arranged by size:
2719 1221 1194 769 225
NspI r_CATG'y
Cuts at: 441 1244 2328 2352 3181 3681 4588 441
Size: 803 1084 24 829 500 907 1981
Fragments arranged by size:
1981 1084 907 829 803 500 24
PasI CC'CwG_GG
Cuts at: 961 1217 3024 3102 961
Size: 256 1807 78 3987
Fragments arranged by size:
3987 1807 256 78
PciI A'CATG_T
Cuts at: 4584 4584
Size: 6128
PflMI CCAn_nnn'nTGG
Cuts at: 1437 1686 1739 2516 1437
Size: 249 53 777 5049
Fragments arranged by size:
5049 777 249 53
PfoI T'CCnGG_A
Cuts at: 662 1517 1829 662
Size: 855 312 4961
Fragments arranged by size:
4961 855 312
PmeI GTTT'AAAC
Cuts at: 3127 3127
Size: 6128
PpiI GAACnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 1099 1131 1915 1947 5267 5299 1099
Size: 32 784 32 3320 32 1928
Fragments arranged by size:
3320 1928 784 32 32 32
PpuMI rG'GwC_Cy
Cuts at: 372 628 2285 372
Size: 256 1657 4215
Fragments arranged by size:
4215 1657 256
PshAI GACnn'nnGTC
Cuts at: 1471 1735 1471
Size: 264 5864
PspOMI G'GGCC_C
Cuts at: 1151 3083 3106 1151
Size: 1932 23 4173
Fragments arranged by size:
4173 1932 23
PspXI vC'TCGA_Gb
Cuts at: 2203 2203
Size: 6128
PstI C_TGCA'G
Cuts at: 850 3175 3860 850
Size: 2325 685 3118
Fragments arranged by size:
3118 2325 685
PvuI CG_AT'CG
Cuts at: 104 104
Size: 6128
PvuII CAG'CTG
Cuts at: 972 2316 3090 3553 3913 972
Size: 1344 774 463 360 3187
Fragments arranged by size:
3187 1344 774 463 360
RsrII CG'GwC_CG
Cuts at: 495 4323 495
Size: 3828 2300
SacI G_AGCT'C
Cuts at: 3142 3142
Size: 6128
SacII CC_GC'GG
Cuts at: 161 188 161
Size: 27 6101
SalI G'TCGA_C
Cuts at: 219 3165 219
Size: 2946 3182
SanDI GG'GwC_CC
Cuts at: 372 628 372
Size: 256 5872
SapI GCTCTTCn'nnn_
Cuts at: 4151 4361 4151
Size: 210 5918
SbfI CC_TGCA'GG
Cuts at: 3175 3175
Size: 6128
ScaI AGT'ACT
Cuts at: 1537 5645 1537
Size: 4108 2020
SexAI A'CCwGG_T
Cuts at: 317 2420 317
Size: 2103 4025
SfcI C'TryA_G
Cuts at: 33 846 1815 3171 3856 4849 5040 33
Size: 813 969 1356 685 993 191 1121
Fragments arranged by size:
1356 1121 993 969 813 685 191
SfiI GGCCn_nnn'nGGCC
Cuts at: 1973 2107 2530 1973
Size: 134 423 5571
Fragments arranged by size:
5571 423 134
SfoI GGC'GCC
Cuts at: 387 3560 3808 387
Size: 3173 248 2707
Fragments arranged by size:
3173 2707 248
SgfI GCG_AT'CGC
Cuts at: 104 104
Size: 6128
SmaI CCC'GGG
Cuts at: 121 1910 3150 121
Size: 1789 1240 3099
Fragments arranged by size:
3099 1789 1240
SmlI C'TyrA_G
Cuts at: 598 1576 1612 2203 3283 4198 4690 4952 598
Size: 978 36 591 1080 915 492 262 1774
Fragments arranged by size:
1774 1080 978 915 591 492 262 36
SpeI A'CTAG_T
Cuts at: 67 67
Size: 6128
SphI G_CATG'C
Cuts at: 441 2328 2352 3181 3681 441
Size: 1887 24 829 500 2888
Fragments arranged by size:
2888 1887 829 500 24
StuI AGG'CCT
Cuts at: 1825 2002 1825
Size: 177 5951
StyI C'CwwG_G
Cuts at: 107 582 725 1685 2687 3262 3616 107
Size: 475 143 960 1002 575 354 2619
Fragments arranged by size:
2619 1002 960 575 475 354 143
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 254 849 2329 2700 3115 3711 3844 4303 4512
Size: 595 1480 371 415 596 133 459 209
Cuts at: 4512 254
Size: 1870
Fragments arranged by size:
1870 1480 596 595 459 415 371 209
133
TatI w'GTAC_w
Cuts at: 842 1535 2249 5643 842
Size: 693 714 3394 1327
Fragments arranged by size:
3394 1327 714 693
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 847 879 958 985 990 1017 1871 1903 5645
Size: 32 79 27 5 27 854 32 3742
Cuts at: 5645 5677 847
Size: 32 1298
Fragments arranged by size:
3742 1298 854 79 32 32 32 27
27 5
Tth111I GACn'n_nGTC
Cuts at: 3925 3925
Size: 6128
XbaI T'CTAG_A
Cuts at: 3159 3159
Size: 6128
XcmI CCAnnnn_n'nnnnTGG
Cuts at: 282 1299 2313 3623 282
Size: 1017 1014 1310 2787
Fragments arranged by size:
2787 1310 1017 1014
XhoI C'TCGA_G
Cuts at: 2203 2203
Size: 6128
XmaI C'CCGG_G
Cuts at: 119 1908 3148 119
Size: 1789 1240 3099
Fragments arranged by size:
3099 1789 1240
Enzymes that do cut:
AarI AccI Acc65I AclI AcuI AflIII AgeI AhdI
AleI AlfI AloI AlwNI ApaI ApaLI ApoI AseI
AsiSI AvaI BamHI BanI BanII BbeI BbsI BbvCI
BcgI BciVI BclI BglI BglII BlpI Bme1580I BmgBI
BmrI BplI BpmI Bpu10I BpuEI BsaI BsaAI BsaBI
BsaHI BsaWI BsaXI BseRI BseYI BsgI BsiEI BsiHKAI
BsmI BsmBI Bsp1286I BspEI BspHI BspMI BsrBI BsrDI
BsrFI BssHII BssSI BstAPI BstBI BstXI BstYI Bsu36I
BtgI BtgZI BtsI DraI DrdI EaeI EagI EarI
EciI EcoICRI Eco57MI EcoNI EcoO109I EcoRI FalI FspI
HaeII Hin4I HincII KasI KpnI MluI MmeI MscI
MslI MspA1I NaeI NarI NcoI NgoMIV NspI PasI
PciI PflMI PfoI PmeI PpiI PpuMI PshAI PspOMI
PspXI PstI PvuI PvuII RsrII SacI SacII SalI
SanDI SapI SbfI ScaI SexAI SfcI SfiI SfoI
SgfI SmaI SmlI SpeI SphI StuI StyI TaqII
TatI TstI Tth111I XbaI XcmI XhoI XmaI
Enzymes that do not cut:
AatII AfeI AflII AscI AvrII BaeI BfrBI BmtI
BsiWI BsrGI BstEII BstZ17I ClaI CspCI DraIII EcoRV
FseI FspAI HindIII HpaI MfeI NdeI NheI NotI
NruI NsiI PacI PmlI PsiI PsrI SgrAI SnaBI
SrfI SspI SwaI XmnI ZraI