Restriction map for
pF1KB7270
(Circular) (Six-base) MAPSORT of: pF1KB7270.seq Check: 9650 from: 1 to: 3752
pF1KB7270 3752 bp
With 182 enzymes: SgfI *
June 20, 2008 10:26 ..
AccI GT'mk_AC
Cuts at: 790 790
Size: 3752
Acc65I G'GTAC_C
Cuts at: 768 768
Size: 3752
AclI AA'CG_TT
Cuts at: 3592 3592
Size: 3752
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 205 1577 2009 2756 3024 205
Size: 1372 432 747 268 933
Fragments arranged by size:
1372 933 747 432 268
AflIII A'CryG_T
Cuts at: 1104 2208 3185 1104
Size: 1104 977 1671
Fragments arranged by size:
1671 1104 977
AgeI A'CCGG_T
Cuts at: 2140 2140
Size: 3752
AlwNI CAG_nnn'CTG
Cuts at: 295 688 1057 2624 295
Size: 393 369 1567 1423
Fragments arranged by size:
1567 1423 393 369
ApaI G_GGCC'C
Cuts at: 140 140
Size: 3752
ApaLI G'TGCA_C
Cuts at: 2522 2522
Size: 3752
ApoI r'AATT_y
Cuts at: 621 734 756 3105 3361 621
Size: 113 22 2349 256 1012
Fragments arranged by size:
2349 1012 256 113 22
AseI AT'TA_AT
Cuts at: 20 20
Size: 3752
AsiSI GCG_AT'CGC
Cuts at: 104 104
Size: 3752
AvaI C'yCGr_G
Cuts at: 132 772 132
Size: 640 3112
AvrII C'CTAG_G
Cuts at: 178 178
Size: 3752
BamHI G'GATC_C
Cuts at: 777 777
Size: 3752
BanI G'GyrC_C
Cuts at: 768 1182 1430 1465 768
Size: 414 248 35 3055
Fragments arranged by size:
3055 414 248 35
BanII G_rGCy'C
Cuts at: 140 253 766 1796 140
Size: 113 513 1030 2096
Fragments arranged by size:
2096 1030 513 113
BbeI G_GCGC'C
Cuts at: 1186 1434 1186
Size: 248 3504
BciVI GTATCCnnnnn_n'
Cuts at: 1650 2411 3752 1650
Size: 761 1341 1650
Fragments arranged by size:
1650 1341 761
BclI T'GATC_A
Cuts at: 1272 1272
Size: 3752
BglII A'GATC_T
Cuts at: 1267 3304 1267
Size: 2037 1715
BlpI GC'TnA_GC
Cuts at: 864 864
Size: 3752
Bme1580I G_kGCm'C
Cuts at: 140 1377 1470 2526 140
Size: 1237 93 1056 1366
Fragments arranged by size:
1366 1237 1056 93
BmrI ACTGGGnnnn_n'
Cuts at: 482 503 964 1045 1380 3524 482
Size: 21 461 81 335 2144 710
Fragments arranged by size:
2144 710 461 335 81 21
BplI GAGnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 2119 2119
Size: 3752
Bpu10I CC'TnA_GC
Cuts at: 113 113
Size: 3752
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 928 1807 2299 2597 928
Size: 879 492 298 2083
Fragments arranged by size:
2083 879 492 298
BsaI GGTCTCn'nnnn_
Cuts at: 3456 3456
Size: 3752
BsaAI yAC'GTr
Cuts at: 1105 1105
Size: 3752
BsaBI GATnn'nnATC
Cuts at: 6 1266 3303 6
Size: 1260 2037 455
Fragments arranged by size:
2037 1260 455
BsaHI Gr'CG_yC
Cuts at: 1183 1431 2133 3629 1183
Size: 248 702 1496 1306
Fragments arranged by size:
1496 1306 702 248
BsaWI w'CCGG_w
Cuts at: 941 1462 2140 2414 2561 2972 941
Size: 521 678 274 147 411 1721
Fragments arranged by size:
1721 678 521 411 274 147
BseYI C'CCAG_C
Cuts at: 1177 1935 2512 3008 1177
Size: 758 577 496 1921
Fragments arranged by size:
1921 758 577 496
BsgI GTGCAGnnnnnnnnnnnnnn_nn'
Cuts at: 597 597
Size: 3752
BsiEI CG_ry'CG
Cuts at: 104 1294 1340 2548 104
Size: 1190 46 1208 1308
Fragments arranged by size:
1308 1208 1190 46
BsiHKAI G_wGCw'C
Cuts at: 766 1544 1734 2526 766
Size: 778 190 792 1992
Fragments arranged by size:
1992 792 778 190
Bsp1286I G_dGCh'C
Cuts at: 140 253 766 1377 1470 1544 1734 1796 2526
Size: 113 513 611 93 74 190 62 730
Cuts at: 2526 140
Size: 1366
Fragments arranged by size:
1366 730 611 513 190 113 93 74
62
BspEI T'CCGG_A
Cuts at: 2972 2972
Size: 3752
BspHI T'CATG_A
Cuts at: 2928 2928
Size: 3752
BspMI ACCTGCnnnn'nnnn_
Cuts at: 802 1016 1318 1699 802
Size: 214 302 381 2855
Fragments arranged by size:
2855 381 302 214
BsrBI CCG'CTC
Cuts at: 50 2044 2098 3582 3747 50
Size: 1994 54 1484 165 55
Fragments arranged by size:
1994 1484 165 55 54
BsrDI GCAATG_nn'
Cuts at: 1664 1664
Size: 3752
BsrFI r'CCGG_y
Cuts at: 1750 1931 2140 1750
Size: 181 209 3362
Fragments arranged by size:
3362 209 181
BsrGI T'GTAC_A
Cuts at: 303 303
Size: 3752
BssSI C'ACGA_G
Cuts at: 2023 2381 2023
Size: 358 3394
BstAPI GCAn_nnn'nTGC
Cuts at: 688 1129 688
Size: 441 3311
BstBI TT'CG_AA
Cuts at: 2113 2113
Size: 3752
BstYI r'GATC_y
Cuts at: 1 777 1244 1267 1602 1848 2860 2946 2958
Size: 776 467 23 335 246 1012 86 12
Cuts at: 2958 3304 1
Size: 346 449
Fragments arranged by size:
1012 776 467 449 346 335 246 86
23 12
BtgI C'CryG_G
Cuts at: 107 107
Size: 3752
BtgZI GCGATGnnnnnnnnnn'nnnn_
Cuts at: 1702 1883 2046 1702
Size: 181 163 3408
Fragments arranged by size:
3408 181 163
BtsI GCAGTG_nn'
Cuts at: 995 995
Size: 3752
DraI TTT'AAA
Cuts at: 751 751
Size: 3752
DrdI GACnn_nn'nnGTC
Cuts at: 1458 2316 1458
Size: 858 2894
EaeI y'GGCC_r
Cuts at: 1337 1511 1902 1929 3274 1337
Size: 174 391 27 1345 1815
Fragments arranged by size:
1815 1345 391 174 27
EagI C'GGCC_G
Cuts at: 1337 1337
Size: 3752
EarI CTCTTCn'nnn_
Cuts at: 1775 1985 1775
Size: 210 3542
EciI GGCGGAnnnnnnnnn_nn'
Cuts at: 1960 2270 2416 3562 1960
Size: 310 146 1146 2150
Fragments arranged by size:
2150 1146 310 146
EcoICRI GAG'CTC
Cuts at: 764 764
Size: 3752
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 205 1577 2009 2756 3024 205
Size: 1372 432 747 268 933
Fragments arranged by size:
1372 933 747 432 268
EcoO109I rG'GnC_Cy
Cuts at: 136 891 136
Size: 755 2997
EcoRI G'AATT_C
Cuts at: 756 756
Size: 3752
FalI AAGnnnnnCTTnnnnnnnn_nnnnn'
Cuts at: 235 610 1771 235
Size: 375 1161 2216
Fragments arranged by size:
2216 1161 375
FspI TGC'GCA
Cuts at: 1533 1533
Size: 3752
HaeII r_GCGC'y
Cuts at: 1186 1434 2456 3435 1186
Size: 248 1022 979 1503
Fragments arranged by size:
1503 1022 979 248
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 283 315 1594 1624 1626 1656 283
Size: 32 1279 30 2 30 2379
Fragments arranged by size:
2379 1279 32 30 30 2
HincII GTy'rAC
Cuts at: 791 791
Size: 3752
KasI G'GCGC_C
Cuts at: 1182 1430 1182
Size: 248 3504
KpnI G_GTAC'C
Cuts at: 772 772
Size: 3752
MluI A'CGCG_T
Cuts at: 3185 3185
Size: 3752
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 299 2423 2607 299
Size: 2124 184 1444
Fragments arranged by size:
2124 1444 184
MscI TGG'CCA
Cuts at: 1513 1513
Size: 3752
MslI CAynn'nnrTG
Cuts at: 1741 1868 1741
Size: 127 3625
MspA1I CmG'CkG
Cuts at: 863 1177 1537 2550 2795 863
Size: 314 360 1013 245 1820
Fragments arranged by size:
1820 1013 360 314 245
NaeI GCC'GGC
Cuts at: 1933 1933
Size: 3752
NarI GG'CG_CC
Cuts at: 1183 1431 1183
Size: 248 3504
NcoI C'CATG_G
Cuts at: 107 107
Size: 3752
NgoMIV G'CCGG_C
Cuts at: 1931 1931
Size: 3752
NspI r_CATG'y
Cuts at: 805 1305 2212 805
Size: 500 907 2345
Fragments arranged by size:
2345 907 500
PciI A'CATG_T
Cuts at: 2208 2208
Size: 3752
PmeI GTTT'AAAC
Cuts at: 751 751
Size: 3752
PpiI GAACnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 2891 2923 2891
Size: 32 3720
PspOMI G'GGCC_C
Cuts at: 136 136
Size: 3752
PstI C_TGCA'G
Cuts at: 693 799 1484 693
Size: 106 685 2961
Fragments arranged by size:
2961 685 106
PvuI CG_AT'CG
Cuts at: 104 104
Size: 3752
PvuII CAG'CTG
Cuts at: 1177 1537 1177
Size: 360 3392
RsrII CG'GwC_CG
Cuts at: 1947 1947
Size: 3752
SacI G_AGCT'C
Cuts at: 766 766
Size: 3752
SalI G'TCGA_C
Cuts at: 789 789
Size: 3752
SapI GCTCTTCn'nnn_
Cuts at: 1775 1985 1775
Size: 210 3542
SbfI CC_TGCA'GG
Cuts at: 799 799
Size: 3752
ScaI AGT'ACT
Cuts at: 513 3269 513
Size: 2756 996
SfcI C'TryA_G
Cuts at: 33 689 795 1480 2473 2664 33
Size: 656 106 685 993 191 1121
Fragments arranged by size:
1121 993 685 656 191 106
SfoI GGC'GCC
Cuts at: 1184 1432 1184
Size: 248 3504
SgfI GCG_AT'CGC
Cuts at: 104 104
Size: 3752
SmaI CCC'GGG
Cuts at: 134 774 134
Size: 640 3112
SmlI C'TyrA_G
Cuts at: 907 1822 2314 2576 907
Size: 915 492 262 2083
Fragments arranged by size:
2083 915 492 262
SpeI A'CTAG_T
Cuts at: 67 67
Size: 3752
SphI G_CATG'C
Cuts at: 805 1305 805
Size: 500 3252
StyI C'CwwG_G
Cuts at: 107 178 886 1240 107
Size: 71 708 354 2619
Fragments arranged by size:
2619 708 354 71
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 1335 1468 1927 2136 1335
Size: 133 459 209 2951
Fragments arranged by size:
2951 459 209 133
TatI w'GTAC_w
Cuts at: 303 511 3267 303
Size: 208 2756 788
Fragments arranged by size:
2756 788 208
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 252 284 3269 3301 252
Size: 32 2985 32 703
Fragments arranged by size:
2985 703 32 32
Tth111I GACn'n_nGTC
Cuts at: 1549 1549
Size: 3752
XbaI T'CTAG_A
Cuts at: 783 783
Size: 3752
XcmI CCAnnnn_n'nnnnTGG
Cuts at: 1247 1247
Size: 3752
XmaI C'CCGG_G
Cuts at: 132 772 132
Size: 640 3112
XmnI GAAnn'nnTTC
Cuts at: 195 218 734 195
Size: 23 516 3213
Fragments arranged by size:
3213 516 23
Enzymes that do cut:
AccI Acc65I AclI AcuI AflIII AgeI AlwNI ApaI
ApaLI ApoI AseI AsiSI AvaI AvrII BamHI BanI
BanII BbeI BciVI BclI BglII BlpI Bme1580I BmrI
BplI Bpu10I BpuEI BsaI BsaAI BsaBI BsaHI BsaWI
BseYI BsgI BsiEI BsiHKAI Bsp1286I BspEI BspHI BspMI
BsrBI BsrDI BsrFI BsrGI BssSI BstAPI BstBI BstYI
BtgI BtgZI BtsI DraI DrdI EaeI EagI EarI
EciI EcoICRI Eco57MI EcoO109I EcoRI FalI FspI HaeII
Hin4I HincII KasI KpnI MluI MmeI MscI MslI
MspA1I NaeI NarI NcoI NgoMIV NspI PciI PmeI
PpiI PspOMI PstI PvuI PvuII RsrII SacI SalI
SapI SbfI ScaI SfcI SfoI SgfI SmaI SmlI
SpeI SphI StyI TaqII TatI TstI Tth111I XbaI
XcmI XmaI XmnI
Enzymes that do not cut:
AarI AatII AfeI AflII AhdI AleI AlfI AloI
AscI BaeI BbsI BbvCI BcgI BfrBI BglI BmgBI
BmtI BpmI BsaXI BseRI BsiWI BsmI BsmBI BssHII
BstEII BstXI BstZ17I Bsu36I ClaI CspCI DraIII EcoNI
EcoRV FseI FspAI HindIII HpaI MfeI NdeI NheI
NotI NruI NsiI PacI PasI PflMI PfoI PmlI
PpuMI PshAI PsiI PspXI PsrI SacII SanDI SexAI
SfiI SgrAI SnaBI SrfI SspI StuI SwaI XhoI
ZraI