Restriction map for
pF1KB7267
(Circular) (Six-base) MAPSORT of: pF1KB7267.seq Check: 2230 from: 1 to: 4076
pF1KB7267 4076 bp
With 182 enzymes: SgfI *
June 20, 2008 10:26 ..
AarI CACCTGCnnnn'nnnn_
Cuts at: 864 864
Size: 4076
AccI GT'mk_AC
Cuts at: 1114 1114
Size: 4076
Acc65I G'GTAC_C
Cuts at: 321 1092 321
Size: 771 3305
AclI AA'CG_TT
Cuts at: 3916 3916
Size: 4076
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 1901 2333 3080 3348 1901
Size: 432 747 268 2629
Fragments arranged by size:
2629 747 432 268
AfeI AGC'GCT
Cuts at: 1021 1021
Size: 4076
AflII C'TTAA_G
Cuts at: 175 175
Size: 4076
AflIII A'CryG_T
Cuts at: 696 1428 2532 3509 696
Size: 732 1104 977 1263
Fragments arranged by size:
1263 1104 977 732
AgeI A'CCGG_T
Cuts at: 324 2464 324
Size: 2140 1936
AlwNI CAG_nnn'CTG
Cuts at: 903 1381 2948 903
Size: 478 1567 2031
Fragments arranged by size:
2031 1567 478
ApaI G_GGCC'C
Cuts at: 526 526
Size: 4076
ApaLI G'TGCA_C
Cuts at: 835 2846 835
Size: 2011 2065
ApoI r'AATT_y
Cuts at: 1080 3429 3685 1080
Size: 2349 256 1471
Fragments arranged by size:
2349 1471 256
AseI AT'TA_AT
Cuts at: 20 20
Size: 4076
AsiSI GCG_AT'CGC
Cuts at: 104 104
Size: 4076
AvaI C'yCGr_G
Cuts at: 127 256 404 412 667 1096 127
Size: 129 148 8 255 429 3107
Fragments arranged by size:
3107 429 255 148 129 8
AvrII C'CTAG_G
Cuts at: 447 736 447
Size: 289 3787
BamHI G'GATC_C
Cuts at: 1101 1101
Size: 4076
BanI G'GyrC_C
Cuts at: 321 327 333 348 408 513 876 1092 1506
Size: 6 6 15 60 105 363 216 414
Cuts at: 1506 1754 1789 321
Size: 248 35 2608
Fragments arranged by size:
2608 414 363 248 216 105 60 35
15 6 6
BanII G_rGCy'C
Cuts at: 263 403 526 1090 2120 263
Size: 140 123 564 1030 2219
Fragments arranged by size:
2219 1030 564 140 123
BbeI G_GCGC'C
Cuts at: 352 412 1510 1758 352
Size: 60 1098 248 2670
Fragments arranged by size:
2670 1098 248 60
BbsI GAAGACnn'nnnn_
Cuts at: 151 151
Size: 4076
BbvCI CC'TCA_GC
Cuts at: 997 997
Size: 4076
BcgI CGAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 742 776 1033 1067 742
Size: 34 257 34 3751
Fragments arranged by size:
3751 257 34 34
BciVI GTATCCnnnnn_n'
Cuts at: 1974 2735 4076 1974
Size: 761 1341 1974
Fragments arranged by size:
1974 1341 761
BclI T'GATC_A
Cuts at: 1596 1596
Size: 4076
BglI GCCn_nnn'nGGC
Cuts at: 210 859 210
Size: 649 3427
BglII A'GATC_T
Cuts at: 1591 3628 1591
Size: 2037 2039
BlpI GC'TnA_GC
Cuts at: 1188 1188
Size: 4076
Bme1580I G_kGCm'C
Cuts at: 376 526 839 1701 1794 2850 376
Size: 150 313 862 93 1056 1602
Fragments arranged by size:
1602 1056 862 313 150 93
BmgBI CAC'GTC
Cuts at: 1051 1051
Size: 4076
BmrI ACTGGGnnnn_n'
Cuts at: 337 1288 1369 1704 3848 337
Size: 951 81 335 2144 565
Fragments arranged by size:
2144 951 565 335 81
BplI GAGnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 2443 2443
Size: 4076
Bpu10I CC'TnA_GC
Cuts at: 865 997 865
Size: 132 3944
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 169 1252 2131 2623 2921 169
Size: 1083 879 492 298 1324
Fragments arranged by size:
1324 1083 879 492 298
BsaI GGTCTCn'nnnn_
Cuts at: 423 3780 423
Size: 3357 719
BsaAI yAC'GTr
Cuts at: 699 1429 699
Size: 730 3346
BsaBI GATnn'nnATC
Cuts at: 6 1590 3627 6
Size: 1584 2037 455
Fragments arranged by size:
2037 1584 455
BsaHI Gr'CG_yC
Cuts at: 349 409 1507 1755 2457 3953 349
Size: 60 1098 248 702 1496 472
Fragments arranged by size:
1496 1098 702 472 248 60
BsaWI w'CCGG_w
Cuts at: 324 1265 1786 2464 2738 2885 3296 324
Size: 941 521 678 274 147 411 1104
Fragments arranged by size:
1104 941 678 521 411 274 147
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 759 759
Size: 4076
BseYI C'CCAG_C
Cuts at: 612 1501 2259 2836 3332 612
Size: 889 758 577 496 1356
Fragments arranged by size:
1356 889 758 577 496
BsgI GTGCAGnnnnnnnnnnnnnn_nn'
Cuts at: 1008 1036 1008
Size: 28 4048
BsiEI CG_ry'CG
Cuts at: 104 356 748 1618 1664 2872 104
Size: 252 392 870 46 1208 1308
Fragments arranged by size:
1308 1208 870 392 252 46
BsiHKAI G_wGCw'C
Cuts at: 839 1090 1868 2058 2850 839
Size: 251 778 190 792 2065
Fragments arranged by size:
2065 792 778 251 190
Bsp1286I G_dGCh'C
Cuts at: 263 376 403 526 839 1090 1701 1794 1868
Size: 113 27 123 313 251 611 93 74
Cuts at: 1868 2058 2120 2850 263
Size: 190 62 730 1489
Fragments arranged by size:
1489 730 611 313 251 190 123 113
93 74 62 27
BspEI T'CCGG_A
Cuts at: 3296 3296
Size: 4076
BspHI T'CATG_A
Cuts at: 3252 3252
Size: 4076
BspMI ACCTGCnnnn'nnnn_
Cuts at: 864 1126 1340 1642 2023 864
Size: 262 214 302 381 2917
Fragments arranged by size:
2917 381 302 262 214
BsrBI CCG'CTC
Cuts at: 50 825 2368 2422 3906 4071 50
Size: 775 1543 54 1484 165 55
Fragments arranged by size:
1543 1484 775 165 55 54
BsrDI GCAATG_nn'
Cuts at: 1988 1988
Size: 4076
BsrFI r'CCGG_y
Cuts at: 204 312 324 330 351 2074 2255 2464 204
Size: 108 12 6 21 1723 181 209 1816
Fragments arranged by size:
1816 1723 209 181 108 21 12 6
BssHII G'CGCG_C
Cuts at: 213 589 213
Size: 376 3700
BssSI C'ACGA_G
Cuts at: 2347 2705 2347
Size: 358 3718
BstAPI GCAn_nnn'nTGC
Cuts at: 1453 1453
Size: 4076
BstBI TT'CG_AA
Cuts at: 2437 2437
Size: 4076
BstXI CCAn_nnnn'nTGG
Cuts at: 517 517
Size: 4076
BstYI r'GATC_y
Cuts at: 1 1101 1568 1591 1926 2172 3184 3270 3282
Size: 1100 467 23 335 246 1012 86 12
Cuts at: 3282 3628 1
Size: 346 449
Fragments arranged by size:
1100 1012 467 449 346 335 246 86
23 12
Bsu36I CC'TnA_GG
Cuts at: 922 922
Size: 4076
BtgI C'CryG_G
Cuts at: 139 139
Size: 4076
BtgZI GCGATGnnnnnnnnnn'nnnn_
Cuts at: 2026 2207 2370 2026
Size: 181 163 3732
Fragments arranged by size:
3732 181 163
BtsI GCAGTG_nn'
Cuts at: 461 1319 461
Size: 858 3218
DraI TTT'AAA
Cuts at: 1075 1075
Size: 4076
DrdI GACnn_nn'nnGTC
Cuts at: 1782 2640 1782
Size: 858 3218
EaeI y'GGCC_r
Cuts at: 353 914 1661 1835 2226 2253 3598 353
Size: 561 747 174 391 27 1345 831
Fragments arranged by size:
1345 831 747 561 391 174 27
EagI C'GGCC_G
Cuts at: 353 1661 353
Size: 1308 2768
EarI CTCTTCn'nnn_
Cuts at: 2099 2309 2099
Size: 210 3866
EciI GGCGGAnnnnnnnnn_nn'
Cuts at: 2284 2594 2740 3886 2284
Size: 310 146 1146 2474
Fragments arranged by size:
2474 1146 310 146
EcoICRI GAG'CTC
Cuts at: 1088 1088
Size: 4076
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 1901 2333 3080 3348 1901
Size: 432 747 268 2629
Fragments arranged by size:
2629 747 432 268
EcoO109I rG'GnC_Cy
Cuts at: 296 522 1215 296
Size: 226 693 3157
Fragments arranged by size:
3157 693 226
EcoRI G'AATT_C
Cuts at: 1080 1080
Size: 4076
FalI AAGnnnnnCTTnnnnnnnn_nnnnn'
Cuts at: 2095 2095
Size: 4076
FspI TGC'GCA
Cuts at: 579 1857 579
Size: 1278 2798
FspAI rTGC'GCAy
Cuts at: 579 579
Size: 4076
HaeII r_GCGC'y
Cuts at: 256 352 412 628 865 1023 1510 1758 2780
Size: 96 60 216 237 158 487 248 1022
Cuts at: 2780 3759 256
Size: 979 573
Fragments arranged by size:
1022 979 573 487 248 237 216 158
96 60
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 1918 1948 1950 1980 1918
Size: 30 2 30 4014
Fragments arranged by size:
4014 30 30 2
HincII GTy'rAC
Cuts at: 1115 1115
Size: 4076
KasI G'GCGC_C
Cuts at: 348 408 1506 1754 348
Size: 60 1098 248 2670
Fragments arranged by size:
2670 1098 248 60
KpnI G_GTAC'C
Cuts at: 325 1096 325
Size: 771 3305
MluI A'CGCG_T
Cuts at: 3509 3509
Size: 4076
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 261 446 2747 2931 261
Size: 185 2301 184 1406
Fragments arranged by size:
2301 1406 185 184
MscI TGG'CCA
Cuts at: 916 1837 916
Size: 921 3155
MslI CAynn'nnrTG
Cuts at: 2065 2192 2065
Size: 127 3949
MspA1I CmG'CkG
Cuts at: 1187 1501 1861 2874 3119 1187
Size: 314 360 1013 245 2144
Fragments arranged by size:
2144 1013 360 314 245
NaeI GCC'GGC
Cuts at: 206 353 2257 206
Size: 147 1904 2025
Fragments arranged by size:
2025 1904 147
NarI GG'CG_CC
Cuts at: 349 409 1507 1755 349
Size: 60 1098 248 2670
Fragments arranged by size:
2670 1098 248 60
NdeI CA'TA_TG
Cuts at: 891 891
Size: 4076
NgoMIV G'CCGG_C
Cuts at: 204 351 2255 204
Size: 147 1904 2025
Fragments arranged by size:
2025 1904 147
NspI r_CATG'y
Cuts at: 1129 1629 2536 1129
Size: 500 907 2669
Fragments arranged by size:
2669 907 500
PciI A'CATG_T
Cuts at: 2532 2532
Size: 4076
PfoI T'CCnGG_A
Cuts at: 542 542
Size: 4076
PmeI GTTT'AAAC
Cuts at: 1075 1075
Size: 4076
PpiI GAACnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 3215 3247 3215
Size: 32 4044
PpuMI rG'GwC_Cy
Cuts at: 296 296
Size: 4076
PspOMI G'GGCC_C
Cuts at: 522 522
Size: 4076
PspXI vC'TCGA_Gb
Cuts at: 127 127
Size: 4076
PstI C_TGCA'G
Cuts at: 311 791 1040 1123 1808 311
Size: 480 249 83 685 2579
Fragments arranged by size:
2579 685 480 249 83
PvuI CG_AT'CG
Cuts at: 104 104
Size: 4076
PvuII CAG'CTG
Cuts at: 1501 1861 1501
Size: 360 3716
RsrII CG'GwC_CG
Cuts at: 2271 2271
Size: 4076
SacI G_AGCT'C
Cuts at: 1090 1090
Size: 4076
SalI G'TCGA_C
Cuts at: 1113 1113
Size: 4076
SapI GCTCTTCn'nnn_
Cuts at: 2099 2309 2099
Size: 210 3866
SbfI CC_TGCA'GG
Cuts at: 1040 1123 1040
Size: 83 3993
ScaI AGT'ACT
Cuts at: 3593 3593
Size: 4076
SfcI C'TryA_G
Cuts at: 33 307 787 975 1036 1119 1804 2797 2988
Size: 274 480 188 61 83 685 993 191
Cuts at: 2988 33
Size: 1121
Fragments arranged by size:
1121 993 685 480 274 191 188 83
61
SfoI GGC'GCC
Cuts at: 350 410 1508 1756 350
Size: 60 1098 248 2670
Fragments arranged by size:
2670 1098 248 60
SgfI GCG_AT'CGC
Cuts at: 104 104
Size: 4076
SmaI CCC'GGG
Cuts at: 669 1098 669
Size: 429 3647
SmlI C'TyrA_G
Cuts at: 127 175 184 1231 2146 2638 2900 127
Size: 48 9 1047 915 492 262 1303
Fragments arranged by size:
1303 1047 915 492 262 48 9
SpeI A'CTAG_T
Cuts at: 67 67
Size: 4076
SphI G_CATG'C
Cuts at: 1129 1629 1129
Size: 500 3576
StyI C'CwwG_G
Cuts at: 447 736 1210 1564 447
Size: 289 474 354 2959
Fragments arranged by size:
2959 474 354 289
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 1659 1792 2251 2460 1659
Size: 133 459 209 3275
Fragments arranged by size:
3275 459 209 133
TatI w'GTAC_w
Cuts at: 3591 3591
Size: 4076
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 124 156 524 556 706 738 3593 3625 124
Size: 32 368 32 150 32 2855 32 575
Fragments arranged by size:
2855 575 368 150 32 32 32 32
Tth111I GACn'n_nGTC
Cuts at: 1873 1873
Size: 4076
XbaI T'CTAG_A
Cuts at: 1107 1107
Size: 4076
XcmI CCAnnnn_n'nnnnTGG
Cuts at: 1571 1571
Size: 4076
XhoI C'TCGA_G
Cuts at: 127 127
Size: 4076
XmaI C'CCGG_G
Cuts at: 667 1096 667
Size: 429 3647
Enzymes that do cut:
AarI AccI Acc65I AclI AcuI AfeI AflII AflIII
AgeI AlwNI ApaI ApaLI ApoI AseI AsiSI AvaI
AvrII BamHI BanI BanII BbeI BbsI BbvCI BcgI
BciVI BclI BglI BglII BlpI Bme1580I BmgBI BmrI
BplI Bpu10I BpuEI BsaI BsaAI BsaBI BsaHI BsaWI
BseRI BseYI BsgI BsiEI BsiHKAI Bsp1286I BspEI BspHI
BspMI BsrBI BsrDI BsrFI BssHII BssSI BstAPI BstBI
BstXI BstYI Bsu36I BtgI BtgZI BtsI DraI DrdI
EaeI EagI EarI EciI EcoICRI Eco57MI EcoO109I EcoRI
FalI FspI FspAI HaeII Hin4I HincII KasI KpnI
MluI MmeI MscI MslI MspA1I NaeI NarI NdeI
NgoMIV NspI PciI PfoI PmeI PpiI PpuMI PspOMI
PspXI PstI PvuI PvuII RsrII SacI SalI SapI
SbfI ScaI SfcI SfoI SgfI SmaI SmlI SpeI
SphI StyI TaqII TatI TstI Tth111I XbaI XcmI
XhoI XmaI
Enzymes that do not cut:
AatII AhdI AleI AlfI AloI AscI BaeI BfrBI
BmtI BpmI BsaXI BsiWI BsmI BsmBI BsrGI BstEII
BstZ17I ClaI CspCI DraIII EcoNI EcoRV FseI HindIII
HpaI MfeI NcoI NheI NotI NruI NsiI PacI
PasI PflMI PmlI PshAI PsiI PsrI SacII SanDI
SexAI SfiI SgrAI SnaBI SrfI SspI StuI SwaI
XmnI ZraI