Restriction map for
pF1KB7266
(Circular) (Six-base) MAPSORT of: pF1KB7266.seq Check: 5472 from: 1 to: 4145
pF1KB7266 4145 bp
With 182 enzymes: SgfI *
June 20, 2008 10:26 ..
AccI GT'mk_AC
Cuts at: 1103 1183 1103
Size: 80 4065
Acc65I G'GTAC_C
Cuts at: 1161 1161
Size: 4145
AclI AA'CG_TT
Cuts at: 3985 3985
Size: 4145
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 391 905 930 1970 2402 3149 3417 391
Size: 514 25 1040 432 747 268 1119
Fragments arranged by size:
1119 1040 747 514 432 268 25
AflIII A'CryG_T
Cuts at: 1497 2601 3578 1497
Size: 1104 977 2064
Fragments arranged by size:
2064 1104 977
AgeI A'CCGG_T
Cuts at: 2533 2533
Size: 4145
AleI CACnn'nnGTG
Cuts at: 662 662
Size: 4145
AlfI GCAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 160 893 160
Size: 733 3412
AlwNI CAG_nnn'CTG
Cuts at: 777 1450 3017 777
Size: 673 1567 1905
Fragments arranged by size:
1905 1567 673
ApaLI G'TGCA_C
Cuts at: 2915 2915
Size: 4145
ApoI r'AATT_y
Cuts at: 250 397 1063 1149 3498 3754 250
Size: 147 666 86 2349 256 641
Fragments arranged by size:
2349 666 641 256 147 86
AseI AT'TA_AT
Cuts at: 20 20
Size: 4145
AsiSI GCG_AT'CGC
Cuts at: 104 104
Size: 4145
AvaI C'yCGr_G
Cuts at: 1165 1165
Size: 4145
BamHI G'GATC_C
Cuts at: 1170 1170
Size: 4145
BanI G'GyrC_C
Cuts at: 1161 1575 1823 1858 1161
Size: 414 248 35 3448
Fragments arranged by size:
3448 414 248 35
BanII G_rGCy'C
Cuts at: 1159 2189 1159
Size: 1030 3115
BbeI G_GCGC'C
Cuts at: 1579 1827 1579
Size: 248 3897
BbsI GAAGACnn'nnnn_
Cuts at: 385 501 385
Size: 116 4029
BbvCI CC'TCA_GC
Cuts at: 921 921
Size: 4145
BciVI GTATCCnnnnn_n'
Cuts at: 858 2043 2804 4145 858
Size: 1185 761 1341 858
Fragments arranged by size:
1341 1185 858 761
BclI T'GATC_A
Cuts at: 1665 1665
Size: 4145
BglII A'GATC_T
Cuts at: 1660 3697 1660
Size: 2037 2108
BlpI GC'TnA_GC
Cuts at: 1257 1257
Size: 4145
Bme1580I G_kGCm'C
Cuts at: 589 1770 1863 2919 589
Size: 1181 93 1056 1815
Fragments arranged by size:
1815 1181 1056 93
BmgBI CAC'GTC
Cuts at: 507 507
Size: 4145
BmrI ACTGGGnnnn_n'
Cuts at: 1357 1438 1773 3917 1357
Size: 81 335 2144 1585
Fragments arranged by size:
2144 1585 335 81
BplI GAGnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 2512 2512
Size: 4145
Bpu10I CC'TnA_GC
Cuts at: 670 921 670
Size: 251 3894
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 776 1321 2200 2692 2990 776
Size: 545 879 492 298 1931
Fragments arranged by size:
1931 879 545 492 298
BsaI GGTCTCn'nnnn_
Cuts at: 3849 3849
Size: 4145
BsaAI yAC'GTr
Cuts at: 1498 1498
Size: 4145
BsaBI GATnn'nnATC
Cuts at: 6 1659 3696 6
Size: 1653 2037 455
Fragments arranged by size:
2037 1653 455
BsaHI Gr'CG_yC
Cuts at: 1576 1824 2526 4022 1576
Size: 248 702 1496 1699
Fragments arranged by size:
1699 1496 702 248
BsaWI w'CCGG_w
Cuts at: 1334 1855 2533 2807 2954 3365 1334
Size: 521 678 274 147 411 2114
Fragments arranged by size:
2114 678 521 411 274 147
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 278 278
Size: 4145
BseYI C'CCAG_C
Cuts at: 1570 2328 2905 3401 1570
Size: 758 577 496 2314
Fragments arranged by size:
2314 758 577 496
BsgI GTGCAGnnnnnnnnnnnnnn_nn'
Cuts at: 625 638 1064 625
Size: 13 426 3706
Fragments arranged by size:
3706 426 13
BsiEI CG_ry'CG
Cuts at: 104 1687 1733 2941 104
Size: 1583 46 1208 1308
Fragments arranged by size:
1583 1308 1208 46
BsiHKAI G_wGCw'C
Cuts at: 1159 1937 2127 2919 1159
Size: 778 190 792 2385
Fragments arranged by size:
2385 792 778 190
Bsp1286I G_dGCh'C
Cuts at: 589 1159 1770 1863 1937 2127 2189 2919 589
Size: 570 611 93 74 190 62 730 1815
Fragments arranged by size:
1815 730 611 570 190 93 74 62
BspEI T'CCGG_A
Cuts at: 3365 3365
Size: 4145
BspHI T'CATG_A
Cuts at: 3321 3321
Size: 4145
BspMI ACCTGCnnnn'nnnn_
Cuts at: 526 698 1195 1409 1711 2092 526
Size: 172 497 214 302 381 2579
Fragments arranged by size:
2579 497 381 302 214 172
BsrBI CCG'CTC
Cuts at: 50 2437 2491 3975 4140 50
Size: 2387 54 1484 165 55
Fragments arranged by size:
2387 1484 165 55 54
BsrDI GCAATG_nn'
Cuts at: 677 1031 2057 677
Size: 354 1026 2765
Fragments arranged by size:
2765 1026 354
BsrFI r'CCGG_y
Cuts at: 154 2143 2324 2533 154
Size: 1989 181 209 1766
Fragments arranged by size:
1989 1766 209 181
BssSI C'ACGA_G
Cuts at: 658 2416 2774 658
Size: 1758 358 2029
Fragments arranged by size:
2029 1758 358
BstAPI GCAn_nnn'nTGC
Cuts at: 1042 1522 1042
Size: 480 3665
BstBI TT'CG_AA
Cuts at: 512 2506 512
Size: 1994 2151
BstYI r'GATC_y
Cuts at: 1 787 888 1170 1637 1660 1995 2241 3253
Size: 786 101 282 467 23 335 246 1012
Cuts at: 3253 3339 3351 3697 1
Size: 86 12 346 449
Fragments arranged by size:
1012 786 467 449 346 335 282 246
101 86 23 12
Bsu36I CC'TnA_GG
Cuts at: 984 984
Size: 4145
BtgI C'CryG_G
Cuts at: 171 641 858 171
Size: 470 217 3458
Fragments arranged by size:
3458 470 217
BtgZI GCGATGnnnnnnnnnn'nnnn_
Cuts at: 2095 2276 2439 2095
Size: 181 163 3801
Fragments arranged by size:
3801 181 163
BtsI GCAGTG_nn'
Cuts at: 1388 1388
Size: 4145
DraI TTT'AAA
Cuts at: 1144 1144
Size: 4145
DrdI GACnn_nn'nnGTC
Cuts at: 1851 2709 1851
Size: 858 3287
EaeI y'GGCC_r
Cuts at: 270 620 1730 1904 2295 2322 3667 270
Size: 350 1110 174 391 27 1345 748
Fragments arranged by size:
1345 1110 748 391 350 174 27
EagI C'GGCC_G
Cuts at: 1730 1730
Size: 4145
EarI CTCTTCn'nnn_
Cuts at: 777 2168 2378 777
Size: 1391 210 2544
Fragments arranged by size:
2544 1391 210
EciI GGCGGAnnnnnnnnn_nn'
Cuts at: 2353 2663 2809 3955 2353
Size: 310 146 1146 2543
Fragments arranged by size:
2543 1146 310 146
EcoICRI GAG'CTC
Cuts at: 1157 1157
Size: 4145
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 391 905 930 1970 2402 3149 3417 391
Size: 514 25 1040 432 747 268 1119
Fragments arranged by size:
1119 1040 747 514 432 268 25
EcoO109I rG'GnC_Cy
Cuts at: 1284 1284
Size: 4145
EcoRI G'AATT_C
Cuts at: 1149 1149
Size: 4145
FalI AAGnnnnnCTTnnnnnnnn_nnnnn'
Cuts at: 748 2164 748
Size: 1416 2729
FspI TGC'GCA
Cuts at: 1926 1926
Size: 4145
HaeII r_GCGC'y
Cuts at: 1579 1827 2849 3828 1579
Size: 248 1022 979 1896
Fragments arranged by size:
1896 1022 979 248
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 194 226 739 771 1987 2017 2019 2049 194
Size: 32 513 32 1216 30 2 30 2290
Fragments arranged by size:
2290 1216 513 32 32 30 30 2
HincII GTy'rAC
Cuts at: 1184 1184
Size: 4145
KasI G'GCGC_C
Cuts at: 1575 1823 1575
Size: 248 3897
KpnI G_GTAC'C
Cuts at: 1165 1165
Size: 4145
MluI A'CGCG_T
Cuts at: 3578 3578
Size: 4145
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 2816 3000 2816
Size: 184 3961
MscI TGG'CCA
Cuts at: 272 622 1906 272
Size: 350 1284 2511
Fragments arranged by size:
2511 1284 350
MslI CAynn'nnrTG
Cuts at: 662 2134 2261 662
Size: 1472 127 2546
Fragments arranged by size:
2546 1472 127
MspA1I CmG'CkG
Cuts at: 883 1256 1570 1930 2943 3188 883
Size: 373 314 360 1013 245 1840
Fragments arranged by size:
1840 1013 373 360 314 245
NaeI GCC'GGC
Cuts at: 156 2326 156
Size: 2170 1975
NarI GG'CG_CC
Cuts at: 1576 1824 1576
Size: 248 3897
NgoMIV G'CCGG_C
Cuts at: 154 2324 154
Size: 2170 1975
NspI r_CATG'y
Cuts at: 1198 1698 2605 1198
Size: 500 907 2738
Fragments arranged by size:
2738 907 500
PciI A'CATG_T
Cuts at: 2601 2601
Size: 4145
PfoI T'CCnGG_A
Cuts at: 770 770
Size: 4145
PmeI GTTT'AAAC
Cuts at: 1144 1144
Size: 4145
PpiI GAACnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 3284 3316 3284
Size: 32 4113
PsiI TTA'TAA
Cuts at: 736 736
Size: 4145
PstI C_TGCA'G
Cuts at: 695 1192 1877 695
Size: 497 685 2963
Fragments arranged by size:
2963 685 497
PvuI CG_AT'CG
Cuts at: 104 104
Size: 4145
PvuII CAG'CTG
Cuts at: 883 1570 1930 883
Size: 687 360 3098
Fragments arranged by size:
3098 687 360
RsrII CG'GwC_CG
Cuts at: 2340 2340
Size: 4145
SacI G_AGCT'C
Cuts at: 1159 1159
Size: 4145
SalI G'TCGA_C
Cuts at: 1182 1182
Size: 4145
SapI GCTCTTCn'nnn_
Cuts at: 2168 2378 2168
Size: 210 3935
SbfI CC_TGCA'GG
Cuts at: 695 1192 695
Size: 497 3648
ScaI AGT'ACT
Cuts at: 3662 3662
Size: 4145
SfcI C'TryA_G
Cuts at: 33 404 691 1188 1873 2866 3057 33
Size: 371 287 497 685 993 191 1121
Fragments arranged by size:
1121 993 685 497 371 287 191
SfoI GGC'GCC
Cuts at: 1577 1825 1577
Size: 248 3897
SgfI GCG_AT'CGC
Cuts at: 104 104
Size: 4145
SmaI CCC'GGG
Cuts at: 1167 1167
Size: 4145
SmlI C'TyrA_G
Cuts at: 791 1300 2215 2707 2969 791
Size: 509 915 492 262 1967
Fragments arranged by size:
1967 915 509 492 262
SpeI A'CTAG_T
Cuts at: 67 67
Size: 4145
SphI G_CATG'C
Cuts at: 1198 1698 1198
Size: 500 3645
SspI AAT'ATT
Cuts at: 743 804 743
Size: 61 4084
StuI AGG'CCT
Cuts at: 536 536
Size: 4145
StyI C'CwwG_G
Cuts at: 1079 1279 1633 1079
Size: 200 354 3591
Fragments arranged by size:
3591 354 200
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 1141 1728 1861 2320 2529 1141
Size: 587 133 459 209 2757
Fragments arranged by size:
2757 587 459 209 133
TatI w'GTAC_w
Cuts at: 3660 3660
Size: 4145
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 474 506 3662 3694 474
Size: 32 3156 32 925
Fragments arranged by size:
3156 925 32 32
Tth111I GACn'n_nGTC
Cuts at: 1942 1942
Size: 4145
XbaI T'CTAG_A
Cuts at: 255 1116 1176 255
Size: 861 60 3224
Fragments arranged by size:
3224 861 60
XcmI CCAnnnn_n'nnnnTGG
Cuts at: 1640 1640
Size: 4145
XmaI C'CCGG_G
Cuts at: 1165 1165
Size: 4145
Enzymes that do cut:
AccI Acc65I AclI AcuI AflIII AgeI AleI AlfI
AlwNI ApaLI ApoI AseI AsiSI AvaI BamHI BanI
BanII BbeI BbsI BbvCI BciVI BclI BglII BlpI
Bme1580I BmgBI BmrI BplI Bpu10I BpuEI BsaI BsaAI
BsaBI BsaHI BsaWI BseRI BseYI BsgI BsiEI BsiHKAI
Bsp1286I BspEI BspHI BspMI BsrBI BsrDI BsrFI BssSI
BstAPI BstBI BstYI Bsu36I BtgI BtgZI BtsI DraI
DrdI EaeI EagI EarI EciI EcoICRI Eco57MI EcoO109I
EcoRI FalI FspI HaeII Hin4I HincII KasI KpnI
MluI MmeI MscI MslI MspA1I NaeI NarI NgoMIV
NspI PciI PfoI PmeI PpiI PsiI PstI PvuI
PvuII RsrII SacI SalI SapI SbfI ScaI SfcI
SfoI SgfI SmaI SmlI SpeI SphI SspI StuI
StyI TaqII TatI TstI Tth111I XbaI XcmI XmaI
Enzymes that do not cut:
AarI AatII AfeI AflII AhdI AloI ApaI AscI
AvrII BaeI BcgI BfrBI BglI BmtI BpmI BsaXI
BsiWI BsmI BsmBI BsrGI BssHII BstEII BstXI BstZ17I
ClaI CspCI DraIII EcoNI EcoRV FseI FspAI HindIII
HpaI MfeI NcoI NdeI NheI NotI NruI NsiI
PacI PasI PflMI PmlI PpuMI PshAI PspOMI PspXI
PsrI SacII SanDI SexAI SfiI SgrAI SnaBI SrfI
SwaI XhoI XmnI ZraI