Restriction map for
pF1KB7264
(Circular) (Six-base) MAPSORT of: pF1KB7264.seq Check: 2072 from: 1 to: 3860
pF1KB7264 3860 bp
With 182 enzymes: SgfI *
June 20, 2008 10:26 ..
AccI GT'mk_AC
Cuts at: 898 898
Size: 3860
Acc65I G'GTAC_C
Cuts at: 876 876
Size: 3860
AclI AA'CG_TT
Cuts at: 3700 3700
Size: 3860
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 851 1685 2117 2864 3132 851
Size: 834 432 747 268 1579
Fragments arranged by size:
1579 834 747 432 268
AflII C'TTAA_G
Cuts at: 246 304 246
Size: 58 3802
AflIII A'CryG_T
Cuts at: 1212 2316 3293 1212
Size: 1104 977 1779
Fragments arranged by size:
1779 1104 977
AgeI A'CCGG_T
Cuts at: 2248 2248
Size: 3860
AlwNI CAG_nnn'CTG
Cuts at: 1165 2732 1165
Size: 1567 2293
ApaLI G'TGCA_C
Cuts at: 2630 2630
Size: 3860
ApoI r'AATT_y
Cuts at: 226 241 387 864 3213 3469 226
Size: 15 146 477 2349 256 617
Fragments arranged by size:
2349 617 477 256 146 15
AseI AT'TA_AT
Cuts at: 20 20
Size: 3860
AsiSI GCG_AT'CGC
Cuts at: 104 104
Size: 3860
AvaI C'yCGr_G
Cuts at: 880 880
Size: 3860
BamHI G'GATC_C
Cuts at: 885 885
Size: 3860
BanI G'GyrC_C
Cuts at: 876 1290 1538 1573 876
Size: 414 248 35 3163
Fragments arranged by size:
3163 414 248 35
BanII G_rGCy'C
Cuts at: 874 1904 874
Size: 1030 2830
BbeI G_GCGC'C
Cuts at: 1294 1542 1294
Size: 248 3612
BbsI GAAGACnn'nnnn_
Cuts at: 674 674
Size: 3860
BbvCI CC'TCA_GC
Cuts at: 415 587 415
Size: 172 3688
BciVI GTATCCnnnnn_n'
Cuts at: 1758 2519 3860 1758
Size: 761 1341 1758
Fragments arranged by size:
1758 1341 761
BclI T'GATC_A
Cuts at: 1380 1380
Size: 3860
BglII A'GATC_T
Cuts at: 1375 3412 1375
Size: 2037 1823
BlpI GC'TnA_GC
Cuts at: 972 972
Size: 3860
Bme1580I G_kGCm'C
Cuts at: 1485 1578 2634 1485
Size: 93 1056 2711
Fragments arranged by size:
2711 1056 93
BmgBI CAC'GTC
Cuts at: 714 714
Size: 3860
BmrI ACTGGGnnnn_n'
Cuts at: 1072 1153 1488 3632 1072
Size: 81 335 2144 1300
Fragments arranged by size:
2144 1300 335 81
BplI GAGnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 2227 2227
Size: 3860
Bpu10I CC'TnA_GC
Cuts at: 415 587 656 415
Size: 172 69 3619
Fragments arranged by size:
3619 172 69
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 436 1036 1915 2407 2705 436
Size: 600 879 492 298 1591
Fragments arranged by size:
1591 879 600 492 298
BsaI GGTCTCn'nnnn_
Cuts at: 3564 3564
Size: 3860
BsaAI yAC'GTr
Cuts at: 597 1213 597
Size: 616 3244
BsaBI GATnn'nnATC
Cuts at: 6 1374 3411 6
Size: 1368 2037 455
Fragments arranged by size:
2037 1368 455
BsaHI Gr'CG_yC
Cuts at: 151 1291 1539 2241 3737 151
Size: 1140 248 702 1496 274
Fragments arranged by size:
1496 1140 702 274 248
BsaWI w'CCGG_w
Cuts at: 1049 1570 2248 2522 2669 3080 1049
Size: 521 678 274 147 411 1829
Fragments arranged by size:
1829 678 521 411 274 147
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 511 631 634 649 652 688 703 706 721
Size: 120 3 15 3 36 15 3 15
Cuts at: 721 739 742 745 511
Size: 18 3 3 3626
Fragments arranged by size:
3626 120 36 18 15 15 15 3
3 3 3 3
BseYI C'CCAG_C
Cuts at: 1285 2043 2620 3116 1285
Size: 758 577 496 2029
Fragments arranged by size:
2029 758 577 496
BsiEI CG_ry'CG
Cuts at: 104 193 1402 1448 2656 104
Size: 89 1209 46 1208 1308
Fragments arranged by size:
1308 1209 1208 89 46
BsiHKAI G_wGCw'C
Cuts at: 874 1652 1842 2634 874
Size: 778 190 792 2100
Fragments arranged by size:
2100 792 778 190
Bsp1286I G_dGCh'C
Cuts at: 874 1485 1578 1652 1842 1904 2634 874
Size: 611 93 74 190 62 730 2100
Fragments arranged by size:
2100 730 611 190 93 74 62
BspEI T'CCGG_A
Cuts at: 3080 3080
Size: 3860
BspHI T'CATG_A
Cuts at: 3036 3036
Size: 3860
BspMI ACCTGCnnnn'nnnn_
Cuts at: 910 1124 1426 1807 910
Size: 214 302 381 2963
Fragments arranged by size:
2963 381 302 214
BsrBI CCG'CTC
Cuts at: 50 2152 2206 3690 3855 50
Size: 2102 54 1484 165 55
Fragments arranged by size:
2102 1484 165 55 54
BsrDI GCAATG_nn'
Cuts at: 1772 1772
Size: 3860
BsrFI r'CCGG_y
Cuts at: 1858 2039 2248 1858
Size: 181 209 3470
Fragments arranged by size:
3470 209 181
BssSI C'ACGA_G
Cuts at: 2131 2489 2131
Size: 358 3502
BstAPI GCAn_nnn'nTGC
Cuts at: 1237 1237
Size: 3860
BstBI TT'CG_AA
Cuts at: 2221 2221
Size: 3860
BstEII G'GTnAC_C
Cuts at: 480 480
Size: 3860
BstXI CCAn_nnnn'nTGG
Cuts at: 114 114
Size: 3860
BstYI r'GATC_y
Cuts at: 1 885 1352 1375 1710 1956 2968 3054 3066
Size: 884 467 23 335 246 1012 86 12
Cuts at: 3066 3412 1
Size: 346 449
Fragments arranged by size:
1012 884 467 449 346 335 246 86
23 12
BtgI C'CryG_G
Cuts at: 107 107
Size: 3860
BtgZI GCGATGnnnnnnnnnn'nnnn_
Cuts at: 1810 1991 2154 1810
Size: 181 163 3516
Fragments arranged by size:
3516 181 163
BtsI GCAGTG_nn'
Cuts at: 1103 1103
Size: 3860
DraI TTT'AAA
Cuts at: 859 859
Size: 3860
DrdI GACnn_nn'nnGTC
Cuts at: 1566 2424 1566
Size: 858 3002
EaeI y'GGCC_r
Cuts at: 1445 1619 2010 2037 3382 1445
Size: 174 391 27 1345 1923
Fragments arranged by size:
1923 1345 391 174 27
EagI C'GGCC_G
Cuts at: 1445 1445
Size: 3860
EarI CTCTTCn'nnn_
Cuts at: 698 779 1883 2093 698
Size: 81 1104 210 2465
Fragments arranged by size:
2465 1104 210 81
EciI GGCGGAnnnnnnnnn_nn'
Cuts at: 2068 2378 2524 3670 2068
Size: 310 146 1146 2258
Fragments arranged by size:
2258 1146 310 146
EcoICRI GAG'CTC
Cuts at: 872 872
Size: 3860
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 851 1685 2117 2864 3132 851
Size: 834 432 747 268 1579
Fragments arranged by size:
1579 834 747 432 268
EcoO109I rG'GnC_Cy
Cuts at: 342 570 603 999 342
Size: 228 33 396 3203
Fragments arranged by size:
3203 396 228 33
EcoRI G'AATT_C
Cuts at: 241 864 241
Size: 623 3237
FalI AAGnnnnnCTTnnnnnnnn_nnnnn'
Cuts at: 805 1879 805
Size: 1074 2786
FspI TGC'GCA
Cuts at: 1641 1641
Size: 3860
HaeII r_GCGC'y
Cuts at: 1294 1542 2564 3543 1294
Size: 248 1022 979 1611
Fragments arranged by size:
1611 1022 979 248
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 1702 1732 1734 1764 1702
Size: 30 2 30 3798
Fragments arranged by size:
3798 30 30 2
HincII GTy'rAC
Cuts at: 899 899
Size: 3860
HindIII A'AGCT_T
Cuts at: 310 310
Size: 3860
KasI G'GCGC_C
Cuts at: 1290 1538 1290
Size: 248 3612
KpnI G_GTAC'C
Cuts at: 880 880
Size: 3860
MfeI C'AATT_G
Cuts at: 471 471
Size: 3860
MluI A'CGCG_T
Cuts at: 3293 3293
Size: 3860
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 2531 2715 2531
Size: 184 3676
MscI TGG'CCA
Cuts at: 1621 1621
Size: 3860
MslI CAynn'nnrTG
Cuts at: 112 1849 1976 112
Size: 1737 127 1996
Fragments arranged by size:
1996 1737 127
MspA1I CmG'CkG
Cuts at: 971 1285 1645 2658 2903 971
Size: 314 360 1013 245 1928
Fragments arranged by size:
1928 1013 360 314 245
NaeI GCC'GGC
Cuts at: 2041 2041
Size: 3860
NarI GG'CG_CC
Cuts at: 1291 1539 1291
Size: 248 3612
NcoI C'CATG_G
Cuts at: 107 107
Size: 3860
NgoMIV G'CCGG_C
Cuts at: 2039 2039
Size: 3860
NspI r_CATG'y
Cuts at: 913 1413 2320 913
Size: 500 907 2453
Fragments arranged by size:
2453 907 500
PciI A'CATG_T
Cuts at: 2316 2316
Size: 3860
PfoI T'CCnGG_A
Cuts at: 176 176
Size: 3860
PmeI GTTT'AAAC
Cuts at: 859 859
Size: 3860
PpiI GAACnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 2999 3031 2999
Size: 32 3828
PshAI GACnn'nnGTC
Cuts at: 185 185
Size: 3860
PsiI TTA'TAA
Cuts at: 749 749
Size: 3860
PstI C_TGCA'G
Cuts at: 907 1592 907
Size: 685 3175
PvuI CG_AT'CG
Cuts at: 104 104
Size: 3860
PvuII CAG'CTG
Cuts at: 1285 1645 1285
Size: 360 3500
RsrII CG'GwC_CG
Cuts at: 2055 2055
Size: 3860
SacI G_AGCT'C
Cuts at: 874 874
Size: 3860
SalI G'TCGA_C
Cuts at: 897 897
Size: 3860
SapI GCTCTTCn'nnn_
Cuts at: 779 1883 2093 779
Size: 1104 210 2546
Fragments arranged by size:
2546 1104 210
SbfI CC_TGCA'GG
Cuts at: 907 907
Size: 3860
ScaI AGT'ACT
Cuts at: 3377 3377
Size: 3860
SfcI C'TryA_G
Cuts at: 33 903 1588 2581 2772 33
Size: 870 685 993 191 1121
Fragments arranged by size:
1121 993 870 685 191
SfoI GGC'GCC
Cuts at: 1292 1540 1292
Size: 248 3612
SgfI GCG_AT'CGC
Cuts at: 104 104
Size: 3860
SmaI CCC'GGG
Cuts at: 882 882
Size: 3860
SmlI C'TyrA_G
Cuts at: 246 304 451 1015 1930 2422 2684 246
Size: 58 147 564 915 492 262 1422
Fragments arranged by size:
1422 915 564 492 262 147 58
SpeI A'CTAG_T
Cuts at: 67 67
Size: 3860
SphI G_CATG'C
Cuts at: 913 1413 913
Size: 500 3360
StuI AGG'CCT
Cuts at: 215 266 215
Size: 51 3809
StyI C'CwwG_G
Cuts at: 107 994 1348 107
Size: 887 354 2619
Fragments arranged by size:
2619 887 354
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 179 1443 1576 2035 2244 179
Size: 1264 133 459 209 1795
Fragments arranged by size:
1795 1264 459 209 133
TatI w'GTAC_w
Cuts at: 250 3375 250
Size: 3125 735
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 3377 3409 3377
Size: 32 3828
Tth111I GACn'n_nGTC
Cuts at: 1657 1657
Size: 3860
XbaI T'CTAG_A
Cuts at: 891 891
Size: 3860
XcmI CCAnnnn_n'nnnnTGG
Cuts at: 1355 1355
Size: 3860
XmaI C'CCGG_G
Cuts at: 880 880
Size: 3860
Enzymes that do cut:
AccI Acc65I AclI AcuI AflII AflIII AgeI AlwNI
ApaLI ApoI AseI AsiSI AvaI BamHI BanI BanII
BbeI BbsI BbvCI BciVI BclI BglII BlpI Bme1580I
BmgBI BmrI BplI Bpu10I BpuEI BsaI BsaAI BsaBI
BsaHI BsaWI BseRI BseYI BsiEI BsiHKAI Bsp1286I BspEI
BspHI BspMI BsrBI BsrDI BsrFI BssSI BstAPI BstBI
BstEII BstXI BstYI BtgI BtgZI BtsI DraI DrdI
EaeI EagI EarI EciI EcoICRI Eco57MI EcoO109I EcoRI
FalI FspI HaeII Hin4I HincII HindIII KasI KpnI
MfeI MluI MmeI MscI MslI MspA1I NaeI NarI
NcoI NgoMIV NspI PciI PfoI PmeI PpiI PshAI
PsiI PstI PvuI PvuII RsrII SacI SalI SapI
SbfI ScaI SfcI SfoI SgfI SmaI SmlI SpeI
SphI StuI StyI TaqII TatI TstI Tth111I XbaI
XcmI XmaI
Enzymes that do not cut:
AarI AatII AfeI AhdI AleI AlfI AloI ApaI
AscI AvrII BaeI BcgI BfrBI BglI BmtI BpmI
BsaXI BsgI BsiWI BsmI BsmBI BsrGI BssHII BstZ17I
Bsu36I ClaI CspCI DraIII EcoNI EcoRV FseI FspAI
HpaI NdeI NheI NotI NruI NsiI PacI PasI
PflMI PmlI PpuMI PspOMI PspXI PsrI SacII SanDI
SexAI SfiI SgrAI SnaBI SrfI SspI SwaI XhoI
XmnI ZraI