Restriction map for
pF1KB7263
(Circular) (Six-base) MAPSORT of: pF1KB7263.seq Check: 4539 from: 1 to: 4058
pF1KB7263 4058 bp
With 182 enzymes: SgfI *
June 20, 2008 10:26 ..
AccI GT'mk_AC
Cuts at: 340 1096 340
Size: 756 3302
Acc65I G'GTAC_C
Cuts at: 1074 1074
Size: 4058
AclI AA'CG_TT
Cuts at: 3898 3898
Size: 4058
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 1883 2315 3062 3330 1883
Size: 432 747 268 2611
Fragments arranged by size:
2611 747 432 268
AflIII A'CryG_T
Cuts at: 832 1410 2514 3491 832
Size: 578 1104 977 1399
Fragments arranged by size:
1399 1104 977 578
AgeI A'CCGG_T
Cuts at: 2446 2446
Size: 4058
AlfI GCAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 417 417
Size: 4058
AlwNI CAG_nnn'CTG
Cuts at: 1363 2930 1363
Size: 1567 2491
ApaLI G'TGCA_C
Cuts at: 2828 2828
Size: 4058
ApoI r'AATT_y
Cuts at: 1019 1062 3411 3667 1019
Size: 43 2349 256 1410
Fragments arranged by size:
2349 1410 256 43
AseI AT'TA_AT
Cuts at: 20 20
Size: 4058
AsiSI GCG_AT'CGC
Cuts at: 104 104
Size: 4058
AvaI C'yCGr_G
Cuts at: 1078 1078
Size: 4058
BaeI ACnnnnGTAyCnnnnnnn_nnnnn'
Cuts at: 821 854 821
Size: 33 4025
BamHI G'GATC_C
Cuts at: 1083 1083
Size: 4058
BanI G'GyrC_C
Cuts at: 1074 1488 1736 1771 1074
Size: 414 248 35 3361
Fragments arranged by size:
3361 414 248 35
BanII G_rGCy'C
Cuts at: 143 1072 2102 143
Size: 929 1030 2099
Fragments arranged by size:
2099 1030 929
BbeI G_GCGC'C
Cuts at: 1492 1740 1492
Size: 248 3810
BciVI GTATCCnnnnn_n'
Cuts at: 849 1956 2717 4058 849
Size: 1107 761 1341 849
Fragments arranged by size:
1341 1107 849 761
BclI T'GATC_A
Cuts at: 1578 1578
Size: 4058
BglII A'GATC_T
Cuts at: 1573 3610 1573
Size: 2037 2021
BlpI GC'TnA_GC
Cuts at: 410 1170 410
Size: 760 3298
Bme1580I G_kGCm'C
Cuts at: 1683 1776 2832 1683
Size: 93 1056 2909
Fragments arranged by size:
2909 1056 93
BmrI ACTGGGnnnn_n'
Cuts at: 961 1270 1351 1686 3830 961
Size: 309 81 335 2144 1189
Fragments arranged by size:
2144 1189 335 309 81
BplI GAGnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 2425 2425
Size: 4058
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 193 365 463 193
Size: 172 98 3788
Fragments arranged by size:
3788 172 98
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 1234 2113 2605 2903 1234
Size: 879 492 298 2389
Fragments arranged by size:
2389 879 492 298
BsaI GGTCTCn'nnnn_
Cuts at: 3762 3762
Size: 4058
BsaAI yAC'GTr
Cuts at: 1411 1411
Size: 4058
BsaBI GATnn'nnATC
Cuts at: 6 1572 3609 6
Size: 1566 2037 455
Fragments arranged by size:
2037 1566 455
BsaHI Gr'CG_yC
Cuts at: 1489 1737 2439 3935 1489
Size: 248 702 1496 1612
Fragments arranged by size:
1612 1496 702 248
BsaWI w'CCGG_w
Cuts at: 1247 1768 2446 2720 2867 3278 1247
Size: 521 678 274 147 411 2027
Fragments arranged by size:
2027 678 521 411 274 147
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 1059 1059
Size: 4058
BseYI C'CCAG_C
Cuts at: 1483 2241 2818 3314 1483
Size: 758 577 496 2227
Fragments arranged by size:
2227 758 577 496
BsgI GTGCAGnnnnnnnnnnnnnn_nn'
Cuts at: 487 487
Size: 4058
BsiEI CG_ry'CG
Cuts at: 104 1600 1646 2854 104
Size: 1496 46 1208 1308
Fragments arranged by size:
1496 1308 1208 46
BsiHKAI G_wGCw'C
Cuts at: 1072 1850 2040 2832 1072
Size: 778 190 792 2298
Fragments arranged by size:
2298 792 778 190
Bsp1286I G_dGCh'C
Cuts at: 143 1072 1683 1776 1850 2040 2102 2832 143
Size: 929 611 93 74 190 62 730 1369
Fragments arranged by size:
1369 929 730 611 190 93 74 62
BspEI T'CCGG_A
Cuts at: 3278 3278
Size: 4058
BspHI T'CATG_A
Cuts at: 3234 3234
Size: 4058
BspMI ACCTGCnnnn'nnnn_
Cuts at: 1108 1322 1624 2005 1108
Size: 214 302 381 3161
Fragments arranged by size:
3161 381 302 214
BsrBI CCG'CTC
Cuts at: 50 2350 2404 3888 4053 50
Size: 2300 54 1484 165 55
Fragments arranged by size:
2300 1484 165 55 54
BsrDI GCAATG_nn'
Cuts at: 633 722 1970 633
Size: 89 1248 2721
Fragments arranged by size:
2721 1248 89
BsrFI r'CCGG_y
Cuts at: 2056 2237 2446 2056
Size: 181 209 3668
Fragments arranged by size:
3668 209 181
BsrGI T'GTAC_A
Cuts at: 679 679
Size: 4058
BssSI C'ACGA_G
Cuts at: 803 2329 2687 803
Size: 1526 358 2174
Fragments arranged by size:
2174 1526 358
BstAPI GCAn_nnn'nTGC
Cuts at: 1435 1435
Size: 4058
BstBI TT'CG_AA
Cuts at: 2419 2419
Size: 4058
BstXI CCAn_nnnn'nTGG
Cuts at: 904 904
Size: 4058
BstYI r'GATC_y
Cuts at: 1 386 1083 1550 1573 1908 2154 3166 3252
Size: 385 697 467 23 335 246 1012 86
Cuts at: 3252 3264 3610 1
Size: 12 346 449
Fragments arranged by size:
1012 697 467 449 385 346 335 246
86 23 12
Bsu36I CC'TnA_GG
Cuts at: 154 1039 154
Size: 885 3173
BtgI C'CryG_G
Cuts at: 107 464 621 107
Size: 357 157 3544
Fragments arranged by size:
3544 357 157
BtgZI GCGATGnnnnnnnnnn'nnnn_
Cuts at: 883 2008 2189 2352 883
Size: 1125 181 163 2589
Fragments arranged by size:
2589 1125 181 163
BtsI GCAGTG_nn'
Cuts at: 320 499 1301 320
Size: 179 802 3077
Fragments arranged by size:
3077 802 179
CspCI CAAnnnnnGTGGnnnnnnnnnn_nn'
Cuts at: 339 374 339
Size: 35 4023
DraI TTT'AAA
Cuts at: 1057 1057
Size: 4058
DraIII CAC_nnn'GTG
Cuts at: 736 736
Size: 4058
DrdI GACnn_nn'nnGTC
Cuts at: 1764 2622 1764
Size: 858 3200
EaeI y'GGCC_r
Cuts at: 461 1643 1817 2208 2235 3580 461
Size: 1182 174 391 27 1345 939
Fragments arranged by size:
1345 1182 939 391 174 27
EagI C'GGCC_G
Cuts at: 1643 1643
Size: 4058
EarI CTCTTCn'nnn_
Cuts at: 208 803 2081 2291 208
Size: 595 1278 210 1975
Fragments arranged by size:
1975 1278 595 210
EciI GGCGGAnnnnnnnnn_nn'
Cuts at: 2266 2576 2722 3868 2266
Size: 310 146 1146 2456
Fragments arranged by size:
2456 1146 310 146
EcoICRI GAG'CTC
Cuts at: 1070 1070
Size: 4058
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 193 365 463 1883 2315 3062 3330 193
Size: 172 98 1420 432 747 268 921
Fragments arranged by size:
1420 921 747 432 268 172 98
EcoO109I rG'GnC_Cy
Cuts at: 1197 1197
Size: 4058
EcoRI G'AATT_C
Cuts at: 1019 1062 1019
Size: 43 4015
FalI AAGnnnnnCTTnnnnnnnn_nnnnn'
Cuts at: 2077 2077
Size: 4058
FspI TGC'GCA
Cuts at: 1839 1839
Size: 4058
HaeII r_GCGC'y
Cuts at: 1492 1740 2762 3741 1492
Size: 248 1022 979 1809
Fragments arranged by size:
1809 1022 979 248
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 594 626 784 816 1900 1930 1932 1962 594
Size: 32 158 32 1084 30 2 30 2690
Fragments arranged by size:
2690 1084 158 32 32 30 30 2
HincII GTy'rAC
Cuts at: 1097 1097
Size: 4058
HindIII A'AGCT_T
Cuts at: 567 567
Size: 4058
KasI G'GCGC_C
Cuts at: 1488 1736 1488
Size: 248 3810
KpnI G_GTAC'C
Cuts at: 1078 1078
Size: 4058
MluI A'CGCG_T
Cuts at: 3491 3491
Size: 4058
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 353 2729 2913 353
Size: 2376 184 1498
Fragments arranged by size:
2376 1498 184
MscI TGG'CCA
Cuts at: 463 1819 463
Size: 1356 2702
MslI CAynn'nnrTG
Cuts at: 626 659 848 937 2047 2174 626
Size: 33 189 89 1110 127 2510
Fragments arranged by size:
2510 1110 189 127 89 33
MspA1I CmG'CkG
Cuts at: 414 1169 1483 1843 2856 3101 414
Size: 755 314 360 1013 245 1371
Fragments arranged by size:
1371 1013 755 360 314 245
NaeI GCC'GGC
Cuts at: 2239 2239
Size: 4058
NarI GG'CG_CC
Cuts at: 1489 1737 1489
Size: 248 3810
NcoI C'CATG_G
Cuts at: 107 464 621 107
Size: 357 157 3544
Fragments arranged by size:
3544 357 157
NgoMIV G'CCGG_C
Cuts at: 2237 2237
Size: 4058
NspI r_CATG'y
Cuts at: 836 1111 1611 2518 836
Size: 275 500 907 2376
Fragments arranged by size:
2376 907 500 275
PciI A'CATG_T
Cuts at: 832 2514 832
Size: 1682 2376
PflMI CCAn_nnn'nTGG
Cuts at: 880 880
Size: 4058
PfoI T'CCnGG_A
Cuts at: 165 440 165
Size: 275 3783
PmeI GTTT'AAAC
Cuts at: 1057 1057
Size: 4058
PpiI GAACnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 3197 3229 3197
Size: 32 4026
PstI C_TGCA'G
Cuts at: 1105 1790 1105
Size: 685 3373
PvuI CG_AT'CG
Cuts at: 104 104
Size: 4058
PvuII CAG'CTG
Cuts at: 414 1483 1843 414
Size: 1069 360 2629
Fragments arranged by size:
2629 1069 360
RsrII CG'GwC_CG
Cuts at: 2253 2253
Size: 4058
SacI G_AGCT'C
Cuts at: 1072 1072
Size: 4058
SalI G'TCGA_C
Cuts at: 1095 1095
Size: 4058
SapI GCTCTTCn'nnn_
Cuts at: 803 2081 2291 803
Size: 1278 210 2570
Fragments arranged by size:
2570 1278 210
SbfI CC_TGCA'GG
Cuts at: 1105 1105
Size: 4058
ScaI AGT'ACT
Cuts at: 3575 3575
Size: 4058
SfcI C'TryA_G
Cuts at: 33 987 1101 1786 2779 2970 33
Size: 954 114 685 993 191 1121
Fragments arranged by size:
1121 993 954 685 191 114
SfoI GGC'GCC
Cuts at: 1490 1738 1490
Size: 248 3810
SgfI GCG_AT'CGC
Cuts at: 104 104
Size: 4058
SmaI CCC'GGG
Cuts at: 1080 1080
Size: 4058
SmlI C'TyrA_G
Cuts at: 1213 2128 2620 2882 1213
Size: 915 492 262 2389
Fragments arranged by size:
2389 915 492 262
SpeI A'CTAG_T
Cuts at: 67 67
Size: 4058
SphI G_CATG'C
Cuts at: 1111 1611 1111
Size: 500 3558
StyI C'CwwG_G
Cuts at: 107 464 621 1192 1546 107
Size: 357 157 571 354 2619
Fragments arranged by size:
2619 571 357 354 157
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 1641 1774 2233 2442 1641
Size: 133 459 209 3257
Fragments arranged by size:
3257 459 209 133
TatI w'GTAC_w
Cuts at: 579 679 3573 579
Size: 100 2894 1064
Fragments arranged by size:
2894 1064 100
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 111 143 3575 3607 111
Size: 32 3432 32 562
Fragments arranged by size:
3432 562 32 32
Tth111I GACn'n_nGTC
Cuts at: 1855 1855
Size: 4058
XbaI T'CTAG_A
Cuts at: 1089 1089
Size: 4058
XcmI CCAnnnn_n'nnnnTGG
Cuts at: 735 1553 735
Size: 818 3240
XmaI C'CCGG_G
Cuts at: 1078 1078
Size: 4058
Enzymes that do cut:
AccI Acc65I AclI AcuI AflIII AgeI AlfI AlwNI
ApaLI ApoI AseI AsiSI AvaI BaeI BamHI BanI
BanII BbeI BciVI BclI BglII BlpI Bme1580I BmrI
BplI BpmI BpuEI BsaI BsaAI BsaBI BsaHI BsaWI
BseRI BseYI BsgI BsiEI BsiHKAI Bsp1286I BspEI BspHI
BspMI BsrBI BsrDI BsrFI BsrGI BssSI BstAPI BstBI
BstXI BstYI Bsu36I BtgI BtgZI BtsI CspCI DraI
DraIII DrdI EaeI EagI EarI EciI EcoICRI Eco57MI
EcoO109I EcoRI FalI FspI HaeII Hin4I HincII HindIII
KasI KpnI MluI MmeI MscI MslI MspA1I NaeI
NarI NcoI NgoMIV NspI PciI PflMI PfoI PmeI
PpiI PstI PvuI PvuII RsrII SacI SalI SapI
SbfI ScaI SfcI SfoI SgfI SmaI SmlI SpeI
SphI StyI TaqII TatI TstI Tth111I XbaI XcmI
XmaI
Enzymes that do not cut:
AarI AatII AfeI AflII AhdI AleI AloI ApaI
AscI AvrII BbsI BbvCI BcgI BfrBI BglI BmgBI
BmtI Bpu10I BsaXI BsiWI BsmI BsmBI BssHII BstEII
BstZ17I ClaI EcoNI EcoRV FseI FspAI HpaI MfeI
NdeI NheI NotI NruI NsiI PacI PasI PmlI
PpuMI PshAI PsiI PspOMI PspXI PsrI SacII SanDI
SexAI SfiI SgrAI SnaBI SrfI SspI StuI SwaI
XhoI XmnI ZraI