Restriction map for
pF1KB7262
(Circular) (Six-base) MAPSORT of: pF1KB7262.seq Check: 7045 from: 1 to: 4466
pF1KB7262 4466 bp
With 182 enzymes: SgfI *
June 20, 2008 10:26 ..
AccI GT'mk_AC
Cuts at: 1504 1504
Size: 4466
Acc65I G'GTAC_C
Cuts at: 1482 1482
Size: 4466
AclI AA'CG_TT
Cuts at: 4306 4306
Size: 4466
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 462 780 859 1433 2291 2723 3470 3738 462
Size: 318 79 574 858 432 747 268 1190
Fragments arranged by size:
1190 858 747 574 432 318 268 79
AflIII A'CryG_T
Cuts at: 197 1818 2922 3899 197
Size: 1621 1104 977 764
Fragments arranged by size:
1621 1104 977 764
AgeI A'CCGG_T
Cuts at: 2854 2854
Size: 4466
AlwNI CAG_nnn'CTG
Cuts at: 1069 1405 1771 3338 1069
Size: 336 366 1567 2197
Fragments arranged by size:
2197 1567 366 336
ApaI G_GGCC'C
Cuts at: 426 426
Size: 4466
ApaLI G'TGCA_C
Cuts at: 3236 3236
Size: 4466
ApoI r'AATT_y
Cuts at: 1470 3819 4075 1470
Size: 2349 256 1861
Fragments arranged by size:
2349 1861 256
AseI AT'TA_AT
Cuts at: 20 20
Size: 4466
AsiSI GCG_AT'CGC
Cuts at: 104 104
Size: 4466
AvaI C'yCGr_G
Cuts at: 115 182 738 1015 1486 115
Size: 67 556 277 471 3095
Fragments arranged by size:
3095 556 471 277 67
BaeI ACnnnnGTAyCnnnnnnn_nnnnn'
Cuts at: 1121 1154 1121
Size: 33 4433
BamHI G'GATC_C
Cuts at: 702 1491 702
Size: 789 3677
BanI G'GyrC_C
Cuts at: 245 304 742 902 1107 1482 1896 2144 2179
Size: 59 438 160 205 375 414 248 35
Cuts at: 2179 245
Size: 2532
Fragments arranged by size:
2532 438 414 375 248 205 160 59
35
BanII G_rGCy'C
Cuts at: 189 426 1480 2510 189
Size: 237 1054 1030 2145
Fragments arranged by size:
2145 1054 1030 237
BbeI G_GCGC'C
Cuts at: 308 1900 2148 308
Size: 1592 248 2626
Fragments arranged by size:
2626 1592 248
BbsI GAAGACnn'nnnn_
Cuts at: 883 1381 883
Size: 498 3968
BbvCI CC'TCA_GC
Cuts at: 763 763
Size: 4466
BciVI GTATCCnnnnn_n'
Cuts at: 1149 2364 3125 4466 1149
Size: 1215 761 1341 1149
Fragments arranged by size:
1341 1215 1149 761
BclI T'GATC_A
Cuts at: 1986 1986
Size: 4466
BglII A'GATC_T
Cuts at: 473 548 1202 1981 4018 473
Size: 75 654 779 2037 921
Fragments arranged by size:
2037 921 779 654 75
BlpI GC'TnA_GC
Cuts at: 1578 1578
Size: 4466
Bme1580I G_kGCm'C
Cuts at: 426 486 745 2091 2184 3240 426
Size: 60 259 1346 93 1056 1652
Fragments arranged by size:
1652 1346 1056 259 93 60
BmrI ACTGGGnnnn_n'
Cuts at: 1678 1759 2094 4238 1678
Size: 81 335 2144 1906
Fragments arranged by size:
2144 1906 335 81
BplI GAGnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 713 2833 713
Size: 2120 2346
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 1427 1427
Size: 4466
Bpu10I CC'TnA_GC
Cuts at: 763 763
Size: 4466
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 1642 2521 3013 3311 1642
Size: 879 492 298 2797
Fragments arranged by size:
2797 879 492 298
BsaI GGTCTCn'nnnn_
Cuts at: 4170 4170
Size: 4466
BsaAI yAC'GTr
Cuts at: 1819 1819
Size: 4466
BsaBI GATnn'nnATC
Cuts at: 6 1980 4017 6
Size: 1974 2037 455
Fragments arranged by size:
2037 1974 455
BsaHI Gr'CG_yC
Cuts at: 305 1897 2145 2847 4343 305
Size: 1592 248 702 1496 428
Fragments arranged by size:
1592 1496 702 428 248
BsaWI w'CCGG_w
Cuts at: 1655 2176 2854 3128 3275 3686 1655
Size: 521 678 274 147 411 2435
Fragments arranged by size:
2435 678 521 411 274 147
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 123 123
Size: 4466
BseYI C'CCAG_C
Cuts at: 141 353 588 721 747 1027 1891 2649 3226
Size: 212 235 133 26 280 864 758 577
Cuts at: 3226 3722 141
Size: 496 885
Fragments arranged by size:
885 864 758 577 496 280 235 212
133 26
BsgI GTGCAGnnnnnnnnnnnnnn_nn'
Cuts at: 519 691 519
Size: 172 4294
BsiEI CG_ry'CG
Cuts at: 104 2008 2054 3262 104
Size: 1904 46 1208 1308
Fragments arranged by size:
1904 1308 1208 46
BsiHKAI G_wGCw'C
Cuts at: 1480 2258 2448 3240 1480
Size: 778 190 792 2706
Fragments arranged by size:
2706 792 778 190
Bsp1286I G_dGCh'C
Cuts at: 189 426 486 745 1480 2091 2184 2258 2448
Size: 237 60 259 735 611 93 74 190
Cuts at: 2448 2510 3240 189
Size: 62 730 1415
Fragments arranged by size:
1415 735 730 611 259 237 190 93
74 62 60
BspEI T'CCGG_A
Cuts at: 3686 3686
Size: 4466
BspHI T'CATG_A
Cuts at: 3642 3642
Size: 4466
BspMI ACCTGCnnnn'nnnn_
Cuts at: 301 577 1166 1516 1730 2032 2413 301
Size: 276 589 350 214 302 381 2354
Fragments arranged by size:
2354 589 381 350 302 276 214
BsrBI CCG'CTC
Cuts at: 50 2758 2812 4296 4461 50
Size: 2708 54 1484 165 55
Fragments arranged by size:
2708 1484 165 55 54
BsrDI GCAATG_nn'
Cuts at: 382 2378 382
Size: 1996 2470
BsrFI r'CCGG_y
Cuts at: 2464 2645 2854 2464
Size: 181 209 4076
Fragments arranged by size:
4076 209 181
BssSI C'ACGA_G
Cuts at: 1180 2737 3095 1180
Size: 1557 358 2551
Fragments arranged by size:
2551 1557 358
BstAPI GCAn_nnn'nTGC
Cuts at: 944 1046 1843 944
Size: 102 797 3567
Fragments arranged by size:
3567 797 102
BstBI TT'CG_AA
Cuts at: 2827 2827
Size: 4466
BstXI CCAn_nnnn'nTGG
Cuts at: 393 393
Size: 4466
BstYI r'GATC_y
Cuts at: 1 473 548 702 1202 1491 1958 1981 2316
Size: 472 75 154 500 289 467 23 335
Cuts at: 2316 2562 3574 3660 3672 4018 1
Size: 246 1012 86 12 346 449
Fragments arranged by size:
1012 500 472 467 449 346 335 289
246 154 86 75 23 12
Bsu36I CC'TnA_GG
Cuts at: 698 698
Size: 4466
BtgI C'CryG_G
Cuts at: 107 213 681 888 107
Size: 106 468 207 3685
Fragments arranged by size:
3685 468 207 106
BtgZI GCGATGnnnnnnnnnn'nnnn_
Cuts at: 2416 2597 2760 2416
Size: 181 163 4122
Fragments arranged by size:
4122 181 163
BtsI GCAGTG_nn'
Cuts at: 345 1709 345
Size: 1364 3102
DraI TTT'AAA
Cuts at: 1465 1465
Size: 4466
DrdI GACnn_nn'nnGTC
Cuts at: 2172 3030 2172
Size: 858 3608
EaeI y'GGCC_r
Cuts at: 960 1071 1422 2051 2225 2616 2643 3988 960
Size: 111 351 629 174 391 27 1345 1438
Fragments arranged by size:
1438 1345 629 391 351 174 111 27
EagI C'GGCC_G
Cuts at: 2051 2051
Size: 4466
EarI CTCTTCn'nnn_
Cuts at: 214 653 2489 2699 214
Size: 439 1836 210 1981
Fragments arranged by size:
1981 1836 439 210
EciI GGCGGAnnnnnnnnn_nn'
Cuts at: 326 2674 2984 3130 4276 326
Size: 2348 310 146 1146 516
Fragments arranged by size:
2348 1146 516 310 146
EcoICRI GAG'CTC
Cuts at: 1478 1478
Size: 4466
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 462 780 859 1427 1433 2291 2723 3470 3738
Size: 318 79 568 6 858 432 747 268
Cuts at: 3738 462
Size: 1190
Fragments arranged by size:
1190 858 747 568 432 318 268 79
6
EcoO109I rG'GnC_Cy
Cuts at: 126 423 1119 1605 126
Size: 297 696 486 2987
Fragments arranged by size:
2987 696 486 297
EcoRI G'AATT_C
Cuts at: 1470 1470
Size: 4466
FalI AAGnnnnnCTTnnnnnnnn_nnnnn'
Cuts at: 1342 2485 1342
Size: 1143 3323
FspI TGC'GCA
Cuts at: 2247 2247
Size: 4466
HaeII r_GCGC'y
Cuts at: 308 770 1900 2148 3170 4149 308
Size: 462 1130 248 1022 979 625
Fragments arranged by size:
1130 1022 979 625 462 248
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 2308 2338 2340 2370 2308
Size: 30 2 30 4404
Fragments arranged by size:
4404 30 30 2
HincII GTy'rAC
Cuts at: 1505 1505
Size: 4466
KasI G'GCGC_C
Cuts at: 304 1896 2144 304
Size: 1592 248 2626
Fragments arranged by size:
2626 1592 248
KpnI G_GTAC'C
Cuts at: 1486 1486
Size: 4466
MluI A'CGCG_T
Cuts at: 3899 3899
Size: 4466
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 1375 3137 3321 1375
Size: 1762 184 2520
Fragments arranged by size:
2520 1762 184
MscI TGG'CCA
Cuts at: 1073 2227 1073
Size: 1154 3312
MslI CAynn'nnrTG
Cuts at: 375 2455 2582 375
Size: 2080 127 2259
Fragments arranged by size:
2259 2080 127
MspA1I CmG'CkG
Cuts at: 533 603 1577 1891 2251 3264 3509 533
Size: 70 974 314 360 1013 245 1490
Fragments arranged by size:
1490 1013 974 360 314 245 70
NaeI GCC'GGC
Cuts at: 2647 2647
Size: 4466
NarI GG'CG_CC
Cuts at: 305 1897 2145 305
Size: 1592 248 2626
Fragments arranged by size:
2626 1592 248
NcoI C'CATG_G
Cuts at: 107 213 681 107
Size: 106 468 3892
Fragments arranged by size:
3892 468 106
NgoMIV G'CCGG_C
Cuts at: 2645 2645
Size: 4466
NspI r_CATG'y
Cuts at: 201 1519 2019 2926 201
Size: 1318 500 907 1741
Fragments arranged by size:
1741 1318 907 500
PciI A'CATG_T
Cuts at: 197 2922 197
Size: 2725 1741
PflMI CCAn_nnn'nTGG
Cuts at: 1405 1405
Size: 4466
PmeI GTTT'AAAC
Cuts at: 1465 1465
Size: 4466
PpiI GAACnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 3605 3637 3605
Size: 32 4434
PpuMI rG'GwC_Cy
Cuts at: 126 1119 126
Size: 993 3473
PspOMI G'GGCC_C
Cuts at: 422 422
Size: 4466
PspXI vC'TCGA_Gb
Cuts at: 182 182
Size: 4466
PstI C_TGCA'G
Cuts at: 532 1513 2198 532
Size: 981 685 2800
Fragments arranged by size:
2800 981 685
PvuI CG_AT'CG
Cuts at: 104 104
Size: 4466
PvuII CAG'CTG
Cuts at: 533 1891 2251 533
Size: 1358 360 2748
Fragments arranged by size:
2748 1358 360
RsrII CG'GwC_CG
Cuts at: 2661 2661
Size: 4466
SacI G_AGCT'C
Cuts at: 1480 1480
Size: 4466
SalI G'TCGA_C
Cuts at: 1503 1503
Size: 4466
SapI GCTCTTCn'nnn_
Cuts at: 2489 2699 2489
Size: 210 4256
SbfI CC_TGCA'GG
Cuts at: 1513 1513
Size: 4466
ScaI AGT'ACT
Cuts at: 3983 3983
Size: 4466
SexAI A'CCwGG_T
Cuts at: 1093 1093
Size: 4466
SfcI C'TryA_G
Cuts at: 33 528 1509 2194 3187 3378 33
Size: 495 981 685 993 191 1121
Fragments arranged by size:
1121 993 981 685 495 191
SfoI GGC'GCC
Cuts at: 306 1898 2146 306
Size: 1592 248 2626
Fragments arranged by size:
2626 1592 248
SgfI GCG_AT'CGC
Cuts at: 104 104
Size: 4466
SmaI CCC'GGG
Cuts at: 740 1488 740
Size: 748 3718
SmlI C'TyrA_G
Cuts at: 182 1621 2536 3028 3290 182
Size: 1439 915 492 262 1358
Fragments arranged by size:
1439 1358 915 492 262
SpeI A'CTAG_T
Cuts at: 67 67
Size: 4466
SphI G_CATG'C
Cuts at: 1519 2019 1519
Size: 500 3966
SrfI GCCC'GGGC
Cuts at: 740 740
Size: 4466
StuI AGG'CCT
Cuts at: 269 625 1186 269
Size: 356 561 3549
Fragments arranged by size:
3549 561 356
StyI C'CwwG_G
Cuts at: 107 213 681 803 1103 1267 1600 1954 107
Size: 106 468 122 300 164 333 354 2619
Fragments arranged by size:
2619 468 354 333 300 164 122 106
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 2049 2182 2641 2850 2049
Size: 133 459 209 3665
Fragments arranged by size:
3665 459 209 133
TatI w'GTAC_w
Cuts at: 275 3981 275
Size: 3706 760
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 1332 1364 1411 1443 3983 4015 1332
Size: 32 47 32 2540 32 1783
Fragments arranged by size:
2540 1783 47 32 32 32
Tth111I GACn'n_nGTC
Cuts at: 2263 2263
Size: 4466
XbaI T'CTAG_A
Cuts at: 1497 1497
Size: 4466
XcmI CCAnnnn_n'nnnnTGG
Cuts at: 1961 1961
Size: 4466
XhoI C'TCGA_G
Cuts at: 182 182
Size: 4466
XmaI C'CCGG_G
Cuts at: 738 1486 738
Size: 748 3718
XmnI GAAnn'nnTTC
Cuts at: 1388 1388
Size: 4466
Enzymes that do cut:
AccI Acc65I AclI AcuI AflIII AgeI AlwNI ApaI
ApaLI ApoI AseI AsiSI AvaI BaeI BamHI BanI
BanII BbeI BbsI BbvCI BciVI BclI BglII BlpI
Bme1580I BmrI BplI BpmI Bpu10I BpuEI BsaI BsaAI
BsaBI BsaHI BsaWI BseRI BseYI BsgI BsiEI BsiHKAI
Bsp1286I BspEI BspHI BspMI BsrBI BsrDI BsrFI BssSI
BstAPI BstBI BstXI BstYI Bsu36I BtgI BtgZI BtsI
DraI DrdI EaeI EagI EarI EciI EcoICRI Eco57MI
EcoO109I EcoRI FalI FspI HaeII Hin4I HincII KasI
KpnI MluI MmeI MscI MslI MspA1I NaeI NarI
NcoI NgoMIV NspI PciI PflMI PmeI PpiI PpuMI
PspOMI PspXI PstI PvuI PvuII RsrII SacI SalI
SapI SbfI ScaI SexAI SfcI SfoI SgfI SmaI
SmlI SpeI SphI SrfI StuI StyI TaqII TatI
TstI Tth111I XbaI XcmI XhoI XmaI XmnI
Enzymes that do not cut:
AarI AatII AfeI AflII AhdI AleI AlfI AloI
AscI AvrII BcgI BfrBI BglI BmgBI BmtI BsaXI
BsiWI BsmI BsmBI BsrGI BssHII BstEII BstZ17I ClaI
CspCI DraIII EcoNI EcoRV FseI FspAI HindIII HpaI
MfeI NdeI NheI NotI NruI NsiI PacI PasI
PfoI PmlI PshAI PsiI PsrI SacII SanDI SfiI
SgrAI SnaBI SspI SwaI ZraI