Restriction map for
pF1KB7260
(Circular) (Six-base) MAPSORT of: pF1KB7260.seq Check: 786 from: 1 to: 4049
pF1KB7260 4049 bp
With 182 enzymes: SgfI *
June 20, 2008 10:26 ..
AccI GT'mk_AC
Cuts at: 1087 1087
Size: 4049
Acc65I G'GTAC_C
Cuts at: 1065 1065
Size: 4049
AclI AA'CG_TT
Cuts at: 3889 3889
Size: 4049
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 1874 2306 3053 3321 1874
Size: 432 747 268 2602
Fragments arranged by size:
2602 747 432 268
AflIII A'CryG_T
Cuts at: 1401 2505 3482 1401
Size: 1104 977 1968
Fragments arranged by size:
1968 1104 977
AgeI A'CCGG_T
Cuts at: 470 2437 470
Size: 1967 2082
AleI CACnn'nnGTG
Cuts at: 527 527
Size: 4049
AlwNI CAG_nnn'CTG
Cuts at: 1354 2921 1354
Size: 1567 2482
ApaLI G'TGCA_C
Cuts at: 2819 2819
Size: 4049
ApoI r'AATT_y
Cuts at: 626 1053 3402 3658 626
Size: 427 2349 256 1017
Fragments arranged by size:
2349 1017 427 256
AseI AT'TA_AT
Cuts at: 20 20
Size: 4049
AsiSI GCG_AT'CGC
Cuts at: 104 104
Size: 4049
AvaI C'yCGr_G
Cuts at: 1069 1069
Size: 4049
BamHI G'GATC_C
Cuts at: 165 1074 165
Size: 909 3140
BanI G'GyrC_C
Cuts at: 1065 1479 1727 1762 1065
Size: 414 248 35 3352
Fragments arranged by size:
3352 414 248 35
BanII G_rGCy'C
Cuts at: 1008 1063 2093 1008
Size: 55 1030 2964
Fragments arranged by size:
2964 1030 55
BbeI G_GCGC'C
Cuts at: 1483 1731 1483
Size: 248 3801
BbvCI CC'TCA_GC
Cuts at: 1021 1021
Size: 4049
BcgI CGAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 318 352 318
Size: 34 4015
BciVI GTATCCnnnnn_n'
Cuts at: 1947 2708 4049 1947
Size: 761 1341 1947
Fragments arranged by size:
1947 1341 761
BclI T'GATC_A
Cuts at: 506 1569 506
Size: 1063 2986
BfrBI ATG'CAT
Cuts at: 600 600
Size: 4049
BglII A'GATC_T
Cuts at: 1564 3601 1564
Size: 2037 2012
BlpI GC'TnA_GC
Cuts at: 1161 1161
Size: 4049
Bme1580I G_kGCm'C
Cuts at: 1674 1767 2823 1674
Size: 93 1056 2900
Fragments arranged by size:
2900 1056 93
BmrI ACTGGGnnnn_n'
Cuts at: 1261 1342 1677 3821 1261
Size: 81 335 2144 1489
Fragments arranged by size:
2144 1489 335 81
BplI GAGnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 2416 2416
Size: 4049
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 646 646
Size: 4049
Bpu10I CC'TnA_GC
Cuts at: 1021 1021
Size: 4049
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 1225 2104 2596 2894 1225
Size: 879 492 298 2380
Fragments arranged by size:
2380 879 492 298
BsaI GGTCTCn'nnnn_
Cuts at: 839 3753 839
Size: 2914 1135
BsaAI yAC'GTr
Cuts at: 1402 1402
Size: 4049
BsaBI GATnn'nnATC
Cuts at: 6 1563 3600 6
Size: 1557 2037 455
Fragments arranged by size:
2037 1557 455
BsaHI Gr'CG_yC
Cuts at: 1480 1728 2430 3926 1480
Size: 248 702 1496 1603
Fragments arranged by size:
1603 1496 702 248
BsaWI w'CCGG_w
Cuts at: 470 1238 1759 2437 2711 2858 3269 470
Size: 768 521 678 274 147 411 1250
Fragments arranged by size:
1250 768 678 521 411 274 147
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 568 1009 568
Size: 441 3608
BseYI C'CCAG_C
Cuts at: 225 723 1474 2232 2809 3305 225
Size: 498 751 758 577 496 969
Fragments arranged by size:
969 758 751 577 498 496
BsgI GTGCAGnnnnnnnnnnnnnn_nn'
Cuts at: 481 481
Size: 4049
BsiEI CG_ry'CG
Cuts at: 104 1591 1637 2845 104
Size: 1487 46 1208 1308
Fragments arranged by size:
1487 1308 1208 46
BsiHKAI G_wGCw'C
Cuts at: 1063 1841 2031 2823 1063
Size: 778 190 792 2289
Fragments arranged by size:
2289 792 778 190
Bsp1286I G_dGCh'C
Cuts at: 1008 1063 1674 1767 1841 2031 2093 2823 1008
Size: 55 611 93 74 190 62 730 2234
Fragments arranged by size:
2234 730 611 190 93 74 62 55
BspEI T'CCGG_A
Cuts at: 3269 3269
Size: 4049
BspHI T'CATG_A
Cuts at: 512 566 3225 512
Size: 54 2659 1336
Fragments arranged by size:
2659 1336 54
BspMI ACCTGCnnnn'nnnn_
Cuts at: 242 1099 1313 1615 1996 242
Size: 857 214 302 381 2295
Fragments arranged by size:
2295 857 381 302 214
BsrBI CCG'CTC
Cuts at: 50 2341 2395 3879 4044 50
Size: 2291 54 1484 165 55
Fragments arranged by size:
2291 1484 165 55 54
BsrDI GCAATG_nn'
Cuts at: 1961 1961
Size: 4049
BsrFI r'CCGG_y
Cuts at: 470 2047 2228 2437 470
Size: 1577 181 209 2082
Fragments arranged by size:
2082 1577 209 181
BsrGI T'GTAC_A
Cuts at: 938 938
Size: 4049
BssSI C'ACGA_G
Cuts at: 2320 2678 2320
Size: 358 3691
BstAPI GCAn_nnn'nTGC
Cuts at: 1426 1426
Size: 4049
BstBI TT'CG_AA
Cuts at: 2410 2410
Size: 4049
BstEII G'GTnAC_C
Cuts at: 948 948
Size: 4049
BstXI CCAn_nnnn'nTGG
Cuts at: 213 351 529 213
Size: 138 178 3733
Fragments arranged by size:
3733 178 138
BstYI r'GATC_y
Cuts at: 1 165 1074 1541 1564 1899 2145 3157 3243
Size: 164 909 467 23 335 246 1012 86
Cuts at: 3243 3255 3601 1
Size: 12 346 449
Fragments arranged by size:
1012 909 467 449 346 335 246 164
86 23 12
Bsu36I CC'TnA_GG
Cuts at: 982 982
Size: 4049
BtgI C'CryG_G
Cuts at: 107 923 107
Size: 816 3233
BtgZI GCGATGnnnnnnnnnn'nnnn_
Cuts at: 1999 2180 2343 1999
Size: 181 163 3705
Fragments arranged by size:
3705 181 163
BtsI GCAGTG_nn'
Cuts at: 1292 1292
Size: 4049
DraI TTT'AAA
Cuts at: 1048 1048
Size: 4049
DrdI GACnn_nn'nnGTC
Cuts at: 1755 2613 1755
Size: 858 3191
EaeI y'GGCC_r
Cuts at: 248 1634 1808 2199 2226 3571 248
Size: 1386 174 391 27 1345 726
Fragments arranged by size:
1386 1345 726 391 174 27
EagI C'GGCC_G
Cuts at: 1634 1634
Size: 4049
EarI CTCTTCn'nnn_
Cuts at: 912 2072 2282 912
Size: 1160 210 2679
Fragments arranged by size:
2679 1160 210
EciI GGCGGAnnnnnnnnn_nn'
Cuts at: 2257 2567 2713 3859 2257
Size: 310 146 1146 2447
Fragments arranged by size:
2447 1146 310 146
EcoICRI GAG'CTC
Cuts at: 1061 1061
Size: 4049
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 646 1874 2306 3053 3321 646
Size: 1228 432 747 268 1374
Fragments arranged by size:
1374 1228 747 432 268
EcoNI CCTnn'n_nnAGG
Cuts at: 403 403
Size: 4049
EcoO109I rG'GnC_Cy
Cuts at: 1188 1188
Size: 4049
EcoRI G'AATT_C
Cuts at: 1053 1053
Size: 4049
FalI AAGnnnnnCTTnnnnnnnn_nnnnn'
Cuts at: 2068 2068
Size: 4049
FspI TGC'GCA
Cuts at: 1830 1830
Size: 4049
HaeII r_GCGC'y
Cuts at: 1483 1731 2753 3732 1483
Size: 248 1022 979 1800
Fragments arranged by size:
1800 1022 979 248
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 585 617 1891 1921 1923 1953 585
Size: 32 1274 30 2 30 2681
Fragments arranged by size:
2681 1274 32 30 30 2
HincII GTy'rAC
Cuts at: 315 1088 315
Size: 773 3276
KasI G'GCGC_C
Cuts at: 1479 1727 1479
Size: 248 3801
KpnI G_GTAC'C
Cuts at: 1069 1069
Size: 4049
MluI A'CGCG_T
Cuts at: 3482 3482
Size: 4049
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 323 2720 2904 323
Size: 2397 184 1468
Fragments arranged by size:
2397 1468 184
MscI TGG'CCA
Cuts at: 250 1810 250
Size: 1560 2489
MslI CAynn'nnrTG
Cuts at: 280 527 955 2038 2165 280
Size: 247 428 1083 127 2164
Fragments arranged by size:
2164 1083 428 247 127
MspA1I CmG'CkG
Cuts at: 666 1160 1474 1834 2847 3092 666
Size: 494 314 360 1013 245 1623
Fragments arranged by size:
1623 1013 494 360 314 245
NaeI GCC'GGC
Cuts at: 2230 2230
Size: 4049
NarI GG'CG_CC
Cuts at: 1480 1728 1480
Size: 248 3801
NcoI C'CATG_G
Cuts at: 107 107
Size: 4049
NgoMIV G'CCGG_C
Cuts at: 2228 2228
Size: 4049
NsiI A_TGCA'T
Cuts at: 602 602
Size: 4049
NspI r_CATG'y
Cuts at: 1102 1602 2509 1102
Size: 500 907 2642
Fragments arranged by size:
2642 907 500
PasI CC'CwG_GG
Cuts at: 1008 1008
Size: 4049
PciI A'CATG_T
Cuts at: 2505 2505
Size: 4049
PmeI GTTT'AAAC
Cuts at: 1048 1048
Size: 4049
PpiI GAACnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 3188 3220 3188
Size: 32 4017
PstI C_TGCA'G
Cuts at: 1096 1781 1096
Size: 685 3364
PvuI CG_AT'CG
Cuts at: 104 104
Size: 4049
PvuII CAG'CTG
Cuts at: 666 1474 1834 666
Size: 808 360 2881
Fragments arranged by size:
2881 808 360
RsrII CG'GwC_CG
Cuts at: 2244 2244
Size: 4049
SacI G_AGCT'C
Cuts at: 1063 1063
Size: 4049
SalI G'TCGA_C
Cuts at: 1086 1086
Size: 4049
SapI GCTCTTCn'nnn_
Cuts at: 2072 2282 2072
Size: 210 3839
SbfI CC_TGCA'GG
Cuts at: 1096 1096
Size: 4049
ScaI AGT'ACT
Cuts at: 3566 3566
Size: 4049
SexAI A'CCwGG_T
Cuts at: 212 212
Size: 4049
SfcI C'TryA_G
Cuts at: 33 175 975 1092 1777 2770 2961 33
Size: 142 800 117 685 993 191 1121
Fragments arranged by size:
1121 993 800 685 191 142 117
SfoI GGC'GCC
Cuts at: 1481 1729 1481
Size: 248 3801
SgfI GCG_AT'CGC
Cuts at: 104 104
Size: 4049
SmaI CCC'GGG
Cuts at: 1071 1071
Size: 4049
SmlI C'TyrA_G
Cuts at: 1204 2119 2611 2873 1204
Size: 915 492 262 2380
Fragments arranged by size:
2380 915 492 262
SpeI A'CTAG_T
Cuts at: 67 67
Size: 4049
SphI G_CATG'C
Cuts at: 1102 1602 1102
Size: 500 3549
StyI C'CwwG_G
Cuts at: 107 741 1183 1537 107
Size: 634 442 354 2619
Fragments arranged by size:
2619 634 442 354
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 382 1632 1765 2224 2433 382
Size: 1250 133 459 209 1998
Fragments arranged by size:
1998 1250 459 209 133
TatI w'GTAC_w
Cuts at: 284 938 3564 284
Size: 654 2626 769
Fragments arranged by size:
2626 769 654
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 3566 3598 3566
Size: 32 4017
Tth111I GACn'n_nGTC
Cuts at: 1846 1846
Size: 4049
XbaI T'CTAG_A
Cuts at: 1080 1080
Size: 4049
XcmI CCAnnnn_n'nnnnTGG
Cuts at: 387 1544 387
Size: 1157 2892
XmaI C'CCGG_G
Cuts at: 1069 1069
Size: 4049
XmnI GAAnn'nnTTC
Cuts at: 771 967 771
Size: 196 3853
Enzymes that do cut:
AccI Acc65I AclI AcuI AflIII AgeI AleI AlwNI
ApaLI ApoI AseI AsiSI AvaI BamHI BanI BanII
BbeI BbvCI BcgI BciVI BclI BfrBI BglII BlpI
Bme1580I BmrI BplI BpmI Bpu10I BpuEI BsaI BsaAI
BsaBI BsaHI BsaWI BseRI BseYI BsgI BsiEI BsiHKAI
Bsp1286I BspEI BspHI BspMI BsrBI BsrDI BsrFI BsrGI
BssSI BstAPI BstBI BstEII BstXI BstYI Bsu36I BtgI
BtgZI BtsI DraI DrdI EaeI EagI EarI EciI
EcoICRI Eco57MI EcoNI EcoO109I EcoRI FalI FspI HaeII
Hin4I HincII KasI KpnI MluI MmeI MscI MslI
MspA1I NaeI NarI NcoI NgoMIV NsiI NspI PasI
PciI PmeI PpiI PstI PvuI PvuII RsrII SacI
SalI SapI SbfI ScaI SexAI SfcI SfoI SgfI
SmaI SmlI SpeI SphI StyI TaqII TatI TstI
Tth111I XbaI XcmI XmaI XmnI
Enzymes that do not cut:
AarI AatII AfeI AflII AhdI AlfI AloI ApaI
AscI AvrII BaeI BbsI BglI BmgBI BmtI BsaXI
BsiWI BsmI BsmBI BssHII BstZ17I ClaI CspCI DraIII
EcoRV FseI FspAI HindIII HpaI MfeI NdeI NheI
NotI NruI PacI PflMI PfoI PmlI PpuMI PshAI
PsiI PspOMI PspXI PsrI SacII SanDI SfiI SgrAI
SnaBI SrfI SspI StuI SwaI XhoI ZraI