Restriction map for
pF1KB7258
(Circular) (Six-base) MAPSORT of: pF1KB7258.seq Check: 1653 from: 1 to: 4046
pF1KB7258 4046 bp
With 182 enzymes: SgfI *
June 20, 2008 10:26 ..
AccI GT'mk_AC
Cuts at: 1084 1084
Size: 4046
Acc65I G'GTAC_C
Cuts at: 712 1062 712
Size: 350 3696
AclI AA'CG_TT
Cuts at: 3886 3886
Size: 4046
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 308 1871 2303 3050 3318 308
Size: 1563 432 747 268 1036
Fragments arranged by size:
1563 1036 747 432 268
AflIII A'CryG_T
Cuts at: 1398 2502 3479 1398
Size: 1104 977 1965
Fragments arranged by size:
1965 1104 977
AgeI A'CCGG_T
Cuts at: 2434 2434
Size: 4046
AlwNI CAG_nnn'CTG
Cuts at: 1351 2918 1351
Size: 1567 2479
ApaLI G'TGCA_C
Cuts at: 2816 2816
Size: 4046
ApoI r'AATT_y
Cuts at: 133 1050 3399 3655 133
Size: 917 2349 256 524
Fragments arranged by size:
2349 917 524 256
AseI AT'TA_AT
Cuts at: 20 20
Size: 4046
AsiSI GCG_AT'CGC
Cuts at: 104 104
Size: 4046
AvaI C'yCGr_G
Cuts at: 1066 1066
Size: 4046
BaeI ACnnnnGTAyCnnnnnnn_nnnnn'
Cuts at: 696 729 696
Size: 33 4013
BamHI G'GATC_C
Cuts at: 1071 1071
Size: 4046
BanI G'GyrC_C
Cuts at: 712 1062 1476 1724 1759 712
Size: 350 414 248 35 2999
Fragments arranged by size:
2999 414 350 248 35
BanII G_rGCy'C
Cuts at: 163 1060 2090 163
Size: 897 1030 2119
Fragments arranged by size:
2119 1030 897
BbeI G_GCGC'C
Cuts at: 1480 1728 1480
Size: 248 3798
BciVI GTATCCnnnnn_n'
Cuts at: 1944 2705 4046 1944
Size: 761 1341 1944
Fragments arranged by size:
1944 1341 761
BclI T'GATC_A
Cuts at: 1566 1566
Size: 4046
BglII A'GATC_T
Cuts at: 434 1561 3598 434
Size: 1127 2037 882
Fragments arranged by size:
2037 1127 882
BlpI GC'TnA_GC
Cuts at: 1158 1158
Size: 4046
Bme1580I G_kGCm'C
Cuts at: 1671 1764 2820 1671
Size: 93 1056 2897
Fragments arranged by size:
2897 1056 93
BmgBI CAC'GTC
Cuts at: 545 545
Size: 4046
BmrI ACTGGGnnnn_n'
Cuts at: 1258 1339 1674 3818 1258
Size: 81 335 2144 1486
Fragments arranged by size:
2144 1486 335 81
BplI GAGnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 2413 2413
Size: 4046
Bpu10I CC'TnA_GC
Cuts at: 253 253
Size: 4046
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 1222 2101 2593 2891 1222
Size: 879 492 298 2377
Fragments arranged by size:
2377 879 492 298
BsaI GGTCTCn'nnnn_
Cuts at: 3750 3750
Size: 4046
BsaAI yAC'GTr
Cuts at: 1399 1399
Size: 4046
BsaBI GATnn'nnATC
Cuts at: 6 439 1560 3597 6
Size: 433 1121 2037 455
Fragments arranged by size:
2037 1121 455 433
BsaHI Gr'CG_yC
Cuts at: 1477 1725 2427 3923 1477
Size: 248 702 1496 1600
Fragments arranged by size:
1600 1496 702 248
BsaWI w'CCGG_w
Cuts at: 1235 1756 2434 2708 2855 3266 1235
Size: 521 678 274 147 411 2015
Fragments arranged by size:
2015 678 521 411 274 147
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 220 220
Size: 4046
BseYI C'CCAG_C
Cuts at: 1471 2229 2806 3302 1471
Size: 758 577 496 2215
Fragments arranged by size:
2215 758 577 496
BsgI GTGCAGnnnnnnnnnnnnnn_nn'
Cuts at: 651 651
Size: 4046
BsiEI CG_ry'CG
Cuts at: 104 1588 1634 2842 104
Size: 1484 46 1208 1308
Fragments arranged by size:
1484 1308 1208 46
BsiHKAI G_wGCw'C
Cuts at: 1060 1838 2028 2820 1060
Size: 778 190 792 2286
Fragments arranged by size:
2286 792 778 190
BsmI GAATG_Cn'
Cuts at: 993 993
Size: 4046
Bsp1286I G_dGCh'C
Cuts at: 163 1060 1671 1764 1838 2028 2090 2820 163
Size: 897 611 93 74 190 62 730 1389
Fragments arranged by size:
1389 897 730 611 190 93 74 62
BspEI T'CCGG_A
Cuts at: 3266 3266
Size: 4046
BspHI T'CATG_A
Cuts at: 506 3222 506
Size: 2716 1330
BspMI ACCTGCnnnn'nnnn_
Cuts at: 1096 1310 1612 1993 1096
Size: 214 302 381 3149
Fragments arranged by size:
3149 381 302 214
BsrBI CCG'CTC
Cuts at: 50 2338 2392 3876 4041 50
Size: 2288 54 1484 165 55
Fragments arranged by size:
2288 1484 165 55 54
BsrDI GCAATG_nn'
Cuts at: 716 830 998 1958 716
Size: 114 168 960 2804
Fragments arranged by size:
2804 960 168 114
BsrFI r'CCGG_y
Cuts at: 2044 2225 2434 2044
Size: 181 209 3656
Fragments arranged by size:
3656 209 181
BsrGI T'GTAC_A
Cuts at: 872 872
Size: 4046
BssSI C'ACGA_G
Cuts at: 2317 2675 2317
Size: 358 3688
BstAPI GCAn_nnn'nTGC
Cuts at: 1423 1423
Size: 4046
BstBI TT'CG_AA
Cuts at: 2407 2407
Size: 4046
BstXI CCAn_nnnn'nTGG
Cuts at: 723 723
Size: 4046
BstYI r'GATC_y
Cuts at: 1 434 1071 1538 1561 1896 2142 3154 3240
Size: 433 637 467 23 335 246 1012 86
Cuts at: 3240 3252 3598 1
Size: 12 346 449
Fragments arranged by size:
1012 637 467 449 433 346 335 246
86 23 12
BtgI C'CryG_G
Cuts at: 107 836 107
Size: 729 3317
BtgZI GCGATGnnnnnnnnnn'nnnn_
Cuts at: 1996 2177 2340 1996
Size: 181 163 3702
Fragments arranged by size:
3702 181 163
BtsI GCAGTG_nn'
Cuts at: 1289 1289
Size: 4046
DraI TTT'AAA
Cuts at: 1045 1045
Size: 4046
DraIII CAC_nnn'GTG
Cuts at: 651 651
Size: 4046
DrdI GACnn_nn'nnGTC
Cuts at: 1752 2610 1752
Size: 858 3188
EaeI y'GGCC_r
Cuts at: 1631 1805 2196 2223 3568 1631
Size: 174 391 27 1345 2109
Fragments arranged by size:
2109 1345 391 174 27
EagI C'GGCC_G
Cuts at: 1631 1631
Size: 4046
EarI CTCTTCn'nnn_
Cuts at: 327 2069 2279 327
Size: 1742 210 2094
Fragments arranged by size:
2094 1742 210
EciI GGCGGAnnnnnnnnn_nn'
Cuts at: 2254 2564 2710 3856 2254
Size: 310 146 1146 2444
Fragments arranged by size:
2444 1146 310 146
EcoICRI GAG'CTC
Cuts at: 1058 1058
Size: 4046
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 308 1871 2303 3050 3318 308
Size: 1563 432 747 268 1036
Fragments arranged by size:
1563 1036 747 432 268
EcoO109I rG'GnC_Cy
Cuts at: 1185 1185
Size: 4046
EcoRI G'AATT_C
Cuts at: 133 1050 133
Size: 917 3129
FalI AAGnnnnnCTTnnnnnnnn_nnnnn'
Cuts at: 2065 2065
Size: 4046
FspI TGC'GCA
Cuts at: 1827 1827
Size: 4046
HaeII r_GCGC'y
Cuts at: 1480 1728 2750 3729 1480
Size: 248 1022 979 1797
Fragments arranged by size:
1797 1022 979 248
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 1888 1918 1920 1950 1888
Size: 30 2 30 3984
Fragments arranged by size:
3984 30 30 2
HincII GTy'rAC
Cuts at: 351 1085 351
Size: 734 3312
KasI G'GCGC_C
Cuts at: 1476 1724 1476
Size: 248 3798
KpnI G_GTAC'C
Cuts at: 716 1066 716
Size: 350 3696
MluI A'CGCG_T
Cuts at: 3479 3479
Size: 4046
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 618 2717 2901 618
Size: 2099 184 1763
Fragments arranged by size:
2099 1763 184
MscI TGG'CCA
Cuts at: 1807 1807
Size: 4046
MslI CAynn'nnrTG
Cuts at: 835 940 2035 2162 835
Size: 105 1095 127 2719
Fragments arranged by size:
2719 1095 127 105
MspA1I CmG'CkG
Cuts at: 1157 1471 1831 2844 3089 1157
Size: 314 360 1013 245 2114
Fragments arranged by size:
2114 1013 360 314 245
NaeI GCC'GGC
Cuts at: 2227 2227
Size: 4046
NarI GG'CG_CC
Cuts at: 1477 1725 1477
Size: 248 3798
NcoI C'CATG_G
Cuts at: 107 836 107
Size: 729 3317
NgoMIV G'CCGG_C
Cuts at: 2225 2225
Size: 4046
NspI r_CATG'y
Cuts at: 396 524 879 1099 1599 2506 396
Size: 128 355 220 500 907 1936
Fragments arranged by size:
1936 907 500 355 220 128
PciI A'CATG_T
Cuts at: 2502 2502
Size: 4046
PmeI GTTT'AAAC
Cuts at: 1045 1045
Size: 4046
PpiI GAACnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 3185 3217 3185
Size: 32 4014
PstI C_TGCA'G
Cuts at: 1093 1778 1093
Size: 685 3361
PvuI CG_AT'CG
Cuts at: 104 104
Size: 4046
PvuII CAG'CTG
Cuts at: 1471 1831 1471
Size: 360 3686
RsrII CG'GwC_CG
Cuts at: 2241 2241
Size: 4046
SacI G_AGCT'C
Cuts at: 1060 1060
Size: 4046
SalI G'TCGA_C
Cuts at: 1083 1083
Size: 4046
SapI GCTCTTCn'nnn_
Cuts at: 2069 2279 2069
Size: 210 3836
SbfI CC_TGCA'GG
Cuts at: 1093 1093
Size: 4046
ScaI AGT'ACT
Cuts at: 3563 3563
Size: 4046
SfcI C'TryA_G
Cuts at: 33 753 1089 1774 2767 2958 33
Size: 720 336 685 993 191 1121
Fragments arranged by size:
1121 993 720 685 336 191
SfoI GGC'GCC
Cuts at: 1478 1726 1478
Size: 248 3798
SgfI GCG_AT'CGC
Cuts at: 104 104
Size: 4046
SmaI CCC'GGG
Cuts at: 1068 1068
Size: 4046
SmlI C'TyrA_G
Cuts at: 1201 2116 2608 2870 1201
Size: 915 492 262 2377
Fragments arranged by size:
2377 915 492 262
SpeI A'CTAG_T
Cuts at: 67 67
Size: 4046
SphI G_CATG'C
Cuts at: 1099 1599 1099
Size: 500 3546
StyI C'CwwG_G
Cuts at: 107 836 1180 1534 107
Size: 729 344 354 2619
Fragments arranged by size:
2619 729 354 344
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 288 1629 1762 2221 2430 288
Size: 1341 133 459 209 1904
Fragments arranged by size:
1904 1341 459 209 133
TatI w'GTAC_w
Cuts at: 411 862 872 3561 411
Size: 451 10 2689 896
Fragments arranged by size:
2689 896 451 10
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 3563 3595 3563
Size: 32 4014
Tth111I GACn'n_nGTC
Cuts at: 1843 1843
Size: 4046
XbaI T'CTAG_A
Cuts at: 1077 1077
Size: 4046
XcmI CCAnnnn_n'nnnnTGG
Cuts at: 381 576 1541 381
Size: 195 965 2886
Fragments arranged by size:
2886 965 195
XmaI C'CCGG_G
Cuts at: 1066 1066
Size: 4046
Enzymes that do cut:
AccI Acc65I AclI AcuI AflIII AgeI AlwNI ApaLI
ApoI AseI AsiSI AvaI BaeI BamHI BanI BanII
BbeI BciVI BclI BglII BlpI Bme1580I BmgBI BmrI
BplI Bpu10I BpuEI BsaI BsaAI BsaBI BsaHI BsaWI
BseRI BseYI BsgI BsiEI BsiHKAI BsmI Bsp1286I BspEI
BspHI BspMI BsrBI BsrDI BsrFI BsrGI BssSI BstAPI
BstBI BstXI BstYI BtgI BtgZI BtsI DraI DraIII
DrdI EaeI EagI EarI EciI EcoICRI Eco57MI EcoO109I
EcoRI FalI FspI HaeII Hin4I HincII KasI KpnI
MluI MmeI MscI MslI MspA1I NaeI NarI NcoI
NgoMIV NspI PciI PmeI PpiI PstI PvuI PvuII
RsrII SacI SalI SapI SbfI ScaI SfcI SfoI
SgfI SmaI SmlI SpeI SphI StyI TaqII TatI
TstI Tth111I XbaI XcmI XmaI
Enzymes that do not cut:
AarI AatII AfeI AflII AhdI AleI AlfI AloI
ApaI AscI AvrII BbsI BbvCI BcgI BfrBI BglI
BmtI BpmI BsaXI BsiWI BsmBI BssHII BstEII BstZ17I
Bsu36I ClaI CspCI EcoNI EcoRV FseI FspAI HindIII
HpaI MfeI NdeI NheI NotI NruI NsiI PacI
PasI PflMI PfoI PmlI PpuMI PshAI PsiI PspOMI
PspXI PsrI SacII SanDI SexAI SfiI SgrAI SnaBI
SrfI SspI StuI SwaI XhoI XmnI ZraI