Restriction map for
pF1KB7256
(Circular) (Six-base) MAPSORT of: pF1KB7256.seq Check: 2028 from: 1 to: 4436
pF1KB7256 4436 bp
With 182 enzymes: SgfI *
June 20, 2008 10:26 ..
AccI GT'mk_AC
Cuts at: 284 656 1474 284
Size: 372 818 3246
Fragments arranged by size:
3246 818 372
Acc65I G'GTAC_C
Cuts at: 356 1452 356
Size: 1096 3340
AclI AA'CG_TT
Cuts at: 4276 4276
Size: 4436
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 942 1261 2261 2693 3440 3708 942
Size: 319 1000 432 747 268 1670
Fragments arranged by size:
1670 1000 747 432 319 268
AfeI AGC'GCT
Cuts at: 774 774
Size: 4436
AflIII A'CryG_T
Cuts at: 968 1788 2892 3869 968
Size: 820 1104 977 1535
Fragments arranged by size:
1535 1104 977 820
AgeI A'CCGG_T
Cuts at: 2824 2824
Size: 4436
AlwNI CAG_nnn'CTG
Cuts at: 1741 3308 1741
Size: 1567 2869
ApaI G_GGCC'C
Cuts at: 453 1179 453
Size: 726 3710
ApaLI G'TGCA_C
Cuts at: 3206 3206
Size: 4436
ApoI r'AATT_y
Cuts at: 161 379 1081 1440 3789 4045 161
Size: 218 702 359 2349 256 552
Fragments arranged by size:
2349 702 552 359 256 218
AseI AT'TA_AT
Cuts at: 20 20
Size: 4436
AsiSI GCG_AT'CGC
Cuts at: 104 104
Size: 4436
AvaI C'yCGr_G
Cuts at: 1456 1456
Size: 4436
BamHI G'GATC_C
Cuts at: 1461 1461
Size: 4436
BanI G'GyrC_C
Cuts at: 356 818 981 1452 1866 2114 2149 356
Size: 462 163 471 414 248 35 2643
Fragments arranged by size:
2643 471 462 414 248 163 35
BanII G_rGCy'C
Cuts at: 453 679 1179 1450 2480 453
Size: 226 500 271 1030 2409
Fragments arranged by size:
2409 1030 500 271 226
BbeI G_GCGC'C
Cuts at: 1870 2118 1870
Size: 248 4188
BbsI GAAGACnn'nnnn_
Cuts at: 656 689 948 656
Size: 33 259 4144
Fragments arranged by size:
4144 259 33
BciVI GTATCCnnnnn_n'
Cuts at: 2334 3095 4436 2334
Size: 761 1341 2334
Fragments arranged by size:
2334 1341 761
BclI T'GATC_A
Cuts at: 1956 1956
Size: 4436
BglI GCCn_nnn'nGGC
Cuts at: 448 448
Size: 4436
BglII A'GATC_T
Cuts at: 422 1951 3988 422
Size: 1529 2037 870
Fragments arranged by size:
2037 1529 870
BlpI GC'TnA_GC
Cuts at: 1056 1548 1056
Size: 492 3944
Bme1580I G_kGCm'C
Cuts at: 453 1019 1179 1416 2061 2154 3210 453
Size: 566 160 237 645 93 1056 1679
Fragments arranged by size:
1679 1056 645 566 237 160 93
BmrI ACTGGGnnnn_n'
Cuts at: 1648 1729 2064 4208 1648
Size: 81 335 2144 1876
Fragments arranged by size:
2144 1876 335 81
BplI GAGnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 1022 2803 1022
Size: 1781 2655
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 381 797 381
Size: 416 4020
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 1612 2491 2983 3281 1612
Size: 879 492 298 2767
Fragments arranged by size:
2767 879 492 298
BsaI GGTCTCn'nnnn_
Cuts at: 243 567 4140 243
Size: 324 3573 539
Fragments arranged by size:
3573 539 324
BsaAI yAC'GTr
Cuts at: 1789 1789
Size: 4436
BsaBI GATnn'nnATC
Cuts at: 6 179 1950 3987 6
Size: 173 1771 2037 455
Fragments arranged by size:
2037 1771 455 173
BsaHI Gr'CG_yC
Cuts at: 1867 2115 2817 4313 1867
Size: 248 702 1496 1990
Fragments arranged by size:
1990 1496 702 248
BsaWI w'CCGG_w
Cuts at: 1625 2146 2824 3098 3245 3656 1625
Size: 521 678 274 147 411 2405
Fragments arranged by size:
2405 678 521 411 274 147
BsaXI ACnnnnnCTCCnnnnnnn_nnn'
Cuts at: 1303 1333 1303
Size: 30 4406
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 687 687
Size: 4436
BseYI C'CCAG_C
Cuts at: 1226 1377 1861 2619 3196 3692 1226
Size: 151 484 758 577 496 1970
Fragments arranged by size:
1970 758 577 496 484 151
BsgI GTGCAGnnnnnnnnnnnnnn_nn'
Cuts at: 591 591
Size: 4436
BsiEI CG_ry'CG
Cuts at: 104 1978 2024 3232 104
Size: 1874 46 1208 1308
Fragments arranged by size:
1874 1308 1208 46
BsiHKAI G_wGCw'C
Cuts at: 1003 1220 1405 1450 2228 2418 3210 1003
Size: 217 185 45 778 190 792 2229
Fragments arranged by size:
2229 792 778 217 190 185 45
BsmI GAATG_Cn'
Cuts at: 632 632
Size: 4436
Bsp1286I G_dGCh'C
Cuts at: 453 679 1003 1019 1179 1220 1405 1416 1450
Size: 226 324 16 160 41 185 11 34
Cuts at: 1450 2061 2154 2228 2418 2480 3210 453
Size: 611 93 74 190 62 730 1679
Fragments arranged by size:
1679 730 611 324 226 190 185 160
93 74 62 41 34 16 11
BspEI T'CCGG_A
Cuts at: 3656 3656
Size: 4436
BspHI T'CATG_A
Cuts at: 3612 3612
Size: 4436
BspMI ACCTGCnnnn'nnnn_
Cuts at: 902 1486 1700 2002 2383 902
Size: 584 214 302 381 2955
Fragments arranged by size:
2955 584 381 302 214
BsrBI CCG'CTC
Cuts at: 50 2728 2782 4266 4431 50
Size: 2678 54 1484 165 55
Fragments arranged by size:
2678 1484 165 55 54
BsrDI GCAATG_nn'
Cuts at: 2348 2348
Size: 4436
BsrFI r'CCGG_y
Cuts at: 2434 2615 2824 2434
Size: 181 209 4046
Fragments arranged by size:
4046 209 181
BssSI C'ACGA_G
Cuts at: 2707 3065 2707
Size: 358 4078
BstAPI GCAn_nnn'nTGC
Cuts at: 1813 1813
Size: 4436
BstBI TT'CG_AA
Cuts at: 2797 2797
Size: 4436
BstXI CCAn_nnnn'nTGG
Cuts at: 558 939 1035 1074 558
Size: 381 96 39 3920
Fragments arranged by size:
3920 381 96 39
BstYI r'GATC_y
Cuts at: 1 422 1382 1461 1928 1951 2286 2532 3544
Size: 421 960 79 467 23 335 246 1012
Cuts at: 3544 3630 3642 3988 1
Size: 86 12 346 449
Fragments arranged by size:
1012 960 467 449 421 346 335 246
86 79 23 12
BtgI C'CryG_G
Cuts at: 985 985
Size: 4436
BtgZI GCGATGnnnnnnnnnn'nnnn_
Cuts at: 2386 2567 2730 2386
Size: 181 163 4092
Fragments arranged by size:
4092 181 163
BtsI GCAGTG_nn'
Cuts at: 1679 1679
Size: 4436
DraI TTT'AAA
Cuts at: 1435 1435
Size: 4436
DraIII CAC_nnn'GTG
Cuts at: 991 1400 991
Size: 409 4027
DrdI GACnn_nn'nnGTC
Cuts at: 298 2142 3000 298
Size: 1844 858 1734
Fragments arranged by size:
1844 1734 858
EaeI y'GGCC_r
Cuts at: 2021 2195 2586 2613 3958 2021
Size: 174 391 27 1345 2499
Fragments arranged by size:
2499 1345 391 174 27
EagI C'GGCC_G
Cuts at: 2021 2021
Size: 4436
EarI CTCTTCn'nnn_
Cuts at: 1360 2459 2669 1360
Size: 1099 210 3127
Fragments arranged by size:
3127 1099 210
EciI GGCGGAnnnnnnnnn_nn'
Cuts at: 2644 2954 3100 4246 2644
Size: 310 146 1146 2834
Fragments arranged by size:
2834 1146 310 146
EcoICRI GAG'CTC
Cuts at: 1448 1448
Size: 4436
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 381 797 942 1261 2261 2693 3440 3708 381
Size: 416 145 319 1000 432 747 268 1109
Fragments arranged by size:
1109 1000 747 432 416 319 268 145
EcoNI CCTnn'n_nnAGG
Cuts at: 669 669
Size: 4436
EcoO109I rG'GnC_Cy
Cuts at: 450 1175 1575 450
Size: 725 400 3311
Fragments arranged by size:
3311 725 400
EcoRI G'AATT_C
Cuts at: 161 1440 161
Size: 1279 3157
EcoRV GAT'ATC
Cuts at: 459 459
Size: 4436
FalI AAGnnnnnCTTnnnnnnnn_nnnnn'
Cuts at: 2455 2455
Size: 4436
FspI TGC'GCA
Cuts at: 814 2217 814
Size: 1403 3033
HaeII r_GCGC'y
Cuts at: 776 1870 2118 3140 4119 776
Size: 1094 248 1022 979 1093
Fragments arranged by size:
1094 1093 1022 979 248
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 2278 2308 2310 2340 2278
Size: 30 2 30 4374
Fragments arranged by size:
4374 30 30 2
HincII GTy'rAC
Cuts at: 966 1475 966
Size: 509 3927
KasI G'GCGC_C
Cuts at: 1866 2114 1866
Size: 248 4188
KpnI G_GTAC'C
Cuts at: 360 1456 360
Size: 1096 3340
MluI A'CGCG_T
Cuts at: 3869 3869
Size: 4436
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 998 3107 3291 998
Size: 2109 184 2143
Fragments arranged by size:
2143 2109 184
MscI TGG'CCA
Cuts at: 2197 2197
Size: 4436
MslI CAynn'nnrTG
Cuts at: 148 556 871 1033 2425 2552 148
Size: 408 315 162 1392 127 2032
Fragments arranged by size:
2032 1392 408 315 162 127
MspA1I CmG'CkG
Cuts at: 1547 1861 2221 3234 3479 1547
Size: 314 360 1013 245 2504
Fragments arranged by size:
2504 1013 360 314 245
NaeI GCC'GGC
Cuts at: 2617 2617
Size: 4436
NarI GG'CG_CC
Cuts at: 1867 2115 1867
Size: 248 4188
NdeI CA'TA_TG
Cuts at: 585 585
Size: 4436
NgoMIV G'CCGG_C
Cuts at: 2615 2615
Size: 4436
NspI r_CATG'y
Cuts at: 405 972 1489 1989 2896 405
Size: 567 517 500 907 1945
Fragments arranged by size:
1945 907 567 517 500
PasI CC'CwG_GG
Cuts at: 1172 1172
Size: 4436
PciI A'CATG_T
Cuts at: 968 2892 968
Size: 1924 2512
PfoI T'CCnGG_A
Cuts at: 388 388
Size: 4436
PmeI GTTT'AAAC
Cuts at: 1435 1435
Size: 4436
PpiI GAACnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 3575 3607 3575
Size: 32 4404
PspOMI G'GGCC_C
Cuts at: 449 1175 449
Size: 726 3710
PstI C_TGCA'G
Cuts at: 159 767 1483 2168 159
Size: 608 716 685 2427
Fragments arranged by size:
2427 716 685 608
PvuI CG_AT'CG
Cuts at: 104 104
Size: 4436
PvuII CAG'CTG
Cuts at: 1861 2221 1861
Size: 360 4076
RsrII CG'GwC_CG
Cuts at: 2631 2631
Size: 4436
SacI G_AGCT'C
Cuts at: 1450 1450
Size: 4436
SalI G'TCGA_C
Cuts at: 1473 1473
Size: 4436
SapI GCTCTTCn'nnn_
Cuts at: 1360 2459 2669 1360
Size: 1099 210 3127
Fragments arranged by size:
3127 1099 210
SbfI CC_TGCA'GG
Cuts at: 1483 1483
Size: 4436
ScaI AGT'ACT
Cuts at: 3953 3953
Size: 4436
SexAI A'CCwGG_T
Cuts at: 977 977
Size: 4436
SfcI C'TryA_G
Cuts at: 33 155 763 1479 2164 3157 3348 33
Size: 122 608 716 685 993 191 1121
Fragments arranged by size:
1121 993 716 685 608 191 122
SfoI GGC'GCC
Cuts at: 1868 2116 1868
Size: 248 4188
SgfI GCG_AT'CGC
Cuts at: 104 104
Size: 4436
SmaI CCC'GGG
Cuts at: 1458 1458
Size: 4436
SmlI C'TyrA_G
Cuts at: 1591 2506 2998 3260 1591
Size: 915 492 262 2767
Fragments arranged by size:
2767 915 492 262
SpeI A'CTAG_T
Cuts at: 67 67
Size: 4436
SphI G_CATG'C
Cuts at: 1489 1989 1489
Size: 500 3936
SspI AAT'ATT
Cuts at: 338 338
Size: 4436
StuI AGG'CCT
Cuts at: 1158 1158
Size: 4436
StyI C'CwwG_G
Cuts at: 269 1570 1924 269
Size: 1301 354 2781
Fragments arranged by size:
2781 1301 354
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 915 1361 2019 2152 2611 2820 915
Size: 446 658 133 459 209 2531
Fragments arranged by size:
2531 658 459 446 209 133
TatI w'GTAC_w
Cuts at: 3951 3951
Size: 4436
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 1303 1335 3953 3985 1303
Size: 32 2618 32 1754
Fragments arranged by size:
2618 1754 32 32
Tth111I GACn'n_nGTC
Cuts at: 2233 2233
Size: 4436
XbaI T'CTAG_A
Cuts at: 1467 1467
Size: 4436
XcmI CCAnnnn_n'nnnnTGG
Cuts at: 285 939 1931 285
Size: 654 992 2790
Fragments arranged by size:
2790 992 654
XmaI C'CCGG_G
Cuts at: 1456 1456
Size: 4436
XmnI GAAnn'nnTTC
Cuts at: 686 686
Size: 4436
Enzymes that do cut:
AccI Acc65I AclI AcuI AfeI AflIII AgeI AlwNI
ApaI ApaLI ApoI AseI AsiSI AvaI BamHI BanI
BanII BbeI BbsI BciVI BclI BglI BglII BlpI
Bme1580I BmrI BplI BpmI BpuEI BsaI BsaAI BsaBI
BsaHI BsaWI BsaXI BseRI BseYI BsgI BsiEI BsiHKAI
BsmI Bsp1286I BspEI BspHI BspMI BsrBI BsrDI BsrFI
BssSI BstAPI BstBI BstXI BstYI BtgI BtgZI BtsI
DraI DraIII DrdI EaeI EagI EarI EciI EcoICRI
Eco57MI EcoNI EcoO109I EcoRI EcoRV FalI FspI HaeII
Hin4I HincII KasI KpnI MluI MmeI MscI MslI
MspA1I NaeI NarI NdeI NgoMIV NspI PasI PciI
PfoI PmeI PpiI PspOMI PstI PvuI PvuII RsrII
SacI SalI SapI SbfI ScaI SexAI SfcI SfoI
SgfI SmaI SmlI SpeI SphI SspI StuI StyI
TaqII TatI TstI Tth111I XbaI XcmI XmaI XmnI
Enzymes that do not cut:
AarI AatII AflII AhdI AleI AlfI AloI AscI
AvrII BaeI BbvCI BcgI BfrBI BmgBI BmtI Bpu10I
BsiWI BsmBI BsrGI BssHII BstEII BstZ17I Bsu36I ClaI
CspCI FseI FspAI HindIII HpaI MfeI NcoI NheI
NotI NruI NsiI PacI PflMI PmlI PpuMI PshAI
PsiI PspXI PsrI SacII SanDI SfiI SgrAI SnaBI
SrfI SwaI XhoI ZraI