Restriction map for
pF1KB7252
(Circular) (Six-base) MAPSORT of: pF1KB7252.seq Check: 3058 from: 1 to: 4556
pF1KB7252 4556 bp
With 182 enzymes: SgfI *
June 20, 2008 10:26 ..
AarI CACCTGCnnnn'nnnn_
Cuts at: 1331 1422 1331
Size: 91 4465
AccI GT'mk_AC
Cuts at: 1594 1594
Size: 4556
Acc65I G'GTAC_C
Cuts at: 1572 1572
Size: 4556
AclI AA'CG_TT
Cuts at: 4396 4396
Size: 4556
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 2381 2813 3560 3828 2381
Size: 432 747 268 3109
Fragments arranged by size:
3109 747 432 268
AflIII A'CryG_T
Cuts at: 1908 3012 3989 1908
Size: 1104 977 2475
Fragments arranged by size:
2475 1104 977
AgeI A'CCGG_T
Cuts at: 2944 2944
Size: 4556
AleI CACnn'nnGTG
Cuts at: 737 737
Size: 4556
AloI GAACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 578 610 881 913 578
Size: 32 271 32 4221
Fragments arranged by size:
4221 271 32 32
AlwNI CAG_nnn'CTG
Cuts at: 217 645 828 1861 3428 217
Size: 428 183 1033 1567 1345
Fragments arranged by size:
1567 1345 1033 428 183
ApaI G_GGCC'C
Cuts at: 157 182 661 805 961 1105 157
Size: 25 479 144 156 144 3608
Fragments arranged by size:
3608 479 156 144 144 25
ApaLI G'TGCA_C
Cuts at: 1001 3326 1001
Size: 2325 2231
ApoI r'AATT_y
Cuts at: 1246 1560 3909 4165 1246
Size: 314 2349 256 1637
Fragments arranged by size:
2349 1637 314 256
AseI AT'TA_AT
Cuts at: 20 20
Size: 4556
AsiSI GCG_AT'CGC
Cuts at: 104 104
Size: 4556
AvaI C'yCGr_G
Cuts at: 719 1489 1576 719
Size: 770 87 3699
Fragments arranged by size:
3699 770 87
BamHI G'GATC_C
Cuts at: 1581 1581
Size: 4556
BanI G'GyrC_C
Cuts at: 1572 1986 2234 2269 1572
Size: 414 248 35 3859
Fragments arranged by size:
3859 414 248 35
BanII G_rGCy'C
Cuts at: 157 182 238 311 434 661 805 935 961
Size: 25 56 73 123 227 144 130 26
Cuts at: 961 970 1105 1230 1346 1570 2600 157
Size: 9 135 125 116 224 1030 2113
Fragments arranged by size:
2113 1030 227 224 144 135 130 125
123 116 73 56 26 25 9
BbeI G_GCGC'C
Cuts at: 1990 2238 1990
Size: 248 4308
BbvCI CC'TCA_GC
Cuts at: 1269 1269
Size: 4556
BciVI GTATCCnnnnn_n'
Cuts at: 2454 3215 4556 2454
Size: 761 1341 2454
Fragments arranged by size:
2454 1341 761
BclI T'GATC_A
Cuts at: 221 2076 221
Size: 1855 2701
BglII A'GATC_T
Cuts at: 1067 2071 4108 1067
Size: 1004 2037 1515
Fragments arranged by size:
2037 1515 1004
BlpI GC'TnA_GC
Cuts at: 206 1511 1668 206
Size: 1305 157 3094
Fragments arranged by size:
3094 1305 157
Bme1580I G_kGCm'C
Cuts at: 157 182 661 805 961 1005 1105 2181 2274
Size: 25 479 144 156 44 100 1076 93
Cuts at: 2274 3330 157
Size: 1056 1383
Fragments arranged by size:
1383 1076 1056 479 156 144 100 93
44 25
BmrI ACTGGGnnnn_n'
Cuts at: 731 1331 1768 1849 2184 4328 731
Size: 600 437 81 335 2144 959
Fragments arranged by size:
2144 959 600 437 335 81
BplI GAGnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 231 2923 231
Size: 2692 1864
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 286 466 769 820 886 1365 1394 286
Size: 180 303 51 66 479 29 3448
Fragments arranged by size:
3448 479 303 180 66 51 29
Bpu10I CC'TnA_GC
Cuts at: 1269 1269
Size: 4556
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 1732 2611 3103 3401 1732
Size: 879 492 298 2887
Fragments arranged by size:
2887 879 492 298
BsaI GGTCTCn'nnnn_
Cuts at: 1365 4260 1365
Size: 2895 1661
BsaAI yAC'GTr
Cuts at: 1909 1909
Size: 4556
BsaBI GATnn'nnATC
Cuts at: 6 2070 4107 6
Size: 2064 2037 455
Fragments arranged by size:
2064 2037 455
BsaHI Gr'CG_yC
Cuts at: 112 1987 2235 2937 4433 112
Size: 1875 248 702 1496 235
Fragments arranged by size:
1875 1496 702 248 235
BsaWI w'CCGG_w
Cuts at: 1745 2266 2944 3218 3365 3776 1745
Size: 521 678 274 147 411 2525
Fragments arranged by size:
2525 678 521 411 274 147
BsaXI ACnnnnnCTCCnnnnnnn_nnn'
Cuts at: 578 605 608 635 997 1000 1027 1030 1066
Size: 27 3 27 362 3 27 3 36
Cuts at: 1066 1096 1112 1142 578
Size: 30 16 30 3992
Fragments arranged by size:
3992 362 36 30 30 27 27 27
16 3 3 3
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 1005 1005
Size: 4556
BseYI C'CCAG_C
Cuts at: 227 433 496 670 928 1033 1981 2739 3316
Size: 206 63 174 258 105 948 758 577
Cuts at: 3316 3812 227
Size: 496 971
Fragments arranged by size:
971 948 758 577 496 258 206 174
105 63
BsgI GTGCAGnnnnnnnnnnnnnn_nn'
Cuts at: 190 190
Size: 4556
BsiEI CG_ry'CG
Cuts at: 104 2098 2144 3352 104
Size: 1994 46 1208 1308
Fragments arranged by size:
1994 1308 1208 46
BsiHKAI G_wGCw'C
Cuts at: 1005 1230 1570 2348 2538 3330 1005
Size: 225 340 778 190 792 2231
Fragments arranged by size:
2231 792 778 340 225 190
BsmBI CGTCTCn'nnnn_
Cuts at: 1086 1086
Size: 4556
Bsp1286I G_dGCh'C
Cuts at: 157 182 238 311 434 661 805 935 961
Size: 25 56 73 123 227 144 130 26
Cuts at: 961 970 1005 1105 1230 1346 1570 2181 2274
Size: 9 35 100 125 116 224 611 93
Cuts at: 2274 2348 2538 2600 3330 157
Size: 74 190 62 730 1383
Fragments arranged by size:
1383 730 611 227 224 190 144 130
125 123 116 100 93 74 73 62
56 35 26 25 9
BspEI T'CCGG_A
Cuts at: 3776 3776
Size: 4556
BspHI T'CATG_A
Cuts at: 3732 3732
Size: 4556
BspMI ACCTGCnnnn'nnnn_
Cuts at: 1331 1422 1606 1820 2122 2503 1331
Size: 91 184 214 302 381 3384
Fragments arranged by size:
3384 381 302 214 184 91
BsrBI CCG'CTC
Cuts at: 50 2848 2902 4386 4551 50
Size: 2798 54 1484 165 55
Fragments arranged by size:
2798 1484 165 55 54
BsrDI GCAATG_nn'
Cuts at: 2468 2468
Size: 4556
BsrFI r'CCGG_y
Cuts at: 2554 2735 2944 2554
Size: 181 209 4166
Fragments arranged by size:
4166 209 181
BssSI C'ACGA_G
Cuts at: 2827 3185 2827
Size: 358 4198
BstAPI GCAn_nnn'nTGC
Cuts at: 1933 1933
Size: 4556
BstBI TT'CG_AA
Cuts at: 2917 2917
Size: 4556
BstXI CCAn_nnnn'nTGG
Cuts at: 504 910 504
Size: 406 4150
BstYI r'GATC_y
Cuts at: 1 1067 1581 2048 2071 2406 2652 3664 3750
Size: 1066 514 467 23 335 246 1012 86
Cuts at: 3750 3762 4108 1
Size: 12 346 449
Fragments arranged by size:
1066 1012 514 467 449 346 335 246
86 23 12
Bsu36I CC'TnA_GG
Cuts at: 513 762 1116 513
Size: 249 354 3953
Fragments arranged by size:
3953 354 249
BtgI C'CryG_G
Cuts at: 975 975
Size: 4556
BtgZI GCGATGnnnnnnnnnn'nnnn_
Cuts at: 2506 2687 2850 2506
Size: 181 163 4212
Fragments arranged by size:
4212 181 163
BtsI GCAGTG_nn'
Cuts at: 1201 1799 1201
Size: 598 3958
DraI TTT'AAA
Cuts at: 1555 1555
Size: 4556
DrdI GACnn_nn'nnGTC
Cuts at: 2262 3120 2262
Size: 858 3698
EaeI y'GGCC_r
Cuts at: 912 2141 2315 2706 2733 4078 912
Size: 1229 174 391 27 1345 1390
Fragments arranged by size:
1390 1345 1229 391 174 27
EagI C'GGCC_G
Cuts at: 2141 2141
Size: 4556
EarI CTCTTCn'nnn_
Cuts at: 2579 2789 2579
Size: 210 4346
EciI GGCGGAnnnnnnnnn_nn'
Cuts at: 2764 3074 3220 4366 2764
Size: 310 146 1146 2954
Fragments arranged by size:
2954 1146 310 146
EcoICRI GAG'CTC
Cuts at: 1228 1568 1228
Size: 340 4216
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 286 466 769 820 886 1365 1394 2381 2813
Size: 180 303 51 66 479 29 987 432
Cuts at: 2813 3560 3828 286
Size: 747 268 1014
Fragments arranged by size:
1014 987 747 479 432 303 268 180
66 51 29
EcoO109I rG'GnC_Cy
Cuts at: 154 161 178 179 642 658 801 802 958
Size: 7 17 1 463 16 143 1 156
Cuts at: 958 1102 1283 1695 154
Size: 144 181 412 3015
Fragments arranged by size:
3015 463 412 181 156 144 143 17
16 7 1 1
EcoRI G'AATT_C
Cuts at: 1246 1560 1246
Size: 314 4242
EcoRV GAT'ATC
Cuts at: 556 556
Size: 4556
FalI AAGnnnnnCTTnnnnnnnn_nnnnn'
Cuts at: 2575 2575
Size: 4556
FspI TGC'GCA
Cuts at: 2337 2337
Size: 4556
HaeII r_GCGC'y
Cuts at: 909 1990 2238 3260 4239 909
Size: 1081 248 1022 979 1226
Fragments arranged by size:
1226 1081 1022 979 248
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 344 376 1066 1098 2398 2428 2430 2460 344
Size: 32 690 32 1300 30 2 30 2440
Fragments arranged by size:
2440 1300 690 32 32 30 30 2
HincII GTy'rAC
Cuts at: 1595 1595
Size: 4556
KasI G'GCGC_C
Cuts at: 1986 2234 1986
Size: 248 4308
KpnI G_GTAC'C
Cuts at: 1576 1576
Size: 4556
MluI A'CGCG_T
Cuts at: 3989 3989
Size: 4556
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 1337 3227 3411 1337
Size: 1890 184 2482
Fragments arranged by size:
2482 1890 184
MscI TGG'CCA
Cuts at: 914 2317 914
Size: 1403 3153
MslI CAynn'nnrTG
Cuts at: 737 1054 1456 1462 2545 2672 737
Size: 317 402 6 1083 127 2621
Fragments arranged by size:
2621 1083 402 317 127 6
MspA1I CmG'CkG
Cuts at: 227 398 1667 1981 2341 3354 3599 227
Size: 171 1269 314 360 1013 245 1184
Fragments arranged by size:
1269 1184 1013 360 314 245 171
NaeI GCC'GGC
Cuts at: 2737 2737
Size: 4556
NarI GG'CG_CC
Cuts at: 1987 2235 1987
Size: 248 4308
NgoMIV G'CCGG_C
Cuts at: 2735 2735
Size: 4556
NspI r_CATG'y
Cuts at: 695 1609 2109 3016 695
Size: 914 500 907 2235
Fragments arranged by size:
2235 914 907 500
PasI CC'CwG_GG
Cuts at: 817 954 817
Size: 137 4419
PciI A'CATG_T
Cuts at: 3012 3012
Size: 4556
PflMI CCAn_nnn'nTGG
Cuts at: 200 200
Size: 4556
PmeI GTTT'AAAC
Cuts at: 1555 1555
Size: 4556
PpiI GAACnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 578 610 3695 3727 578
Size: 32 3085 32 1407
Fragments arranged by size:
3085 1407 32 32
PpuMI rG'GwC_Cy
Cuts at: 161 1283 161
Size: 1122 3434
PspOMI G'GGCC_C
Cuts at: 153 178 657 801 957 1101 153
Size: 25 479 144 156 144 3608
Fragments arranged by size:
3608 479 156 144 144 25
PstI C_TGCA'G
Cuts at: 420 800 1603 2288 420
Size: 380 803 685 2688
Fragments arranged by size:
2688 803 685 380
PvuI CG_AT'CG
Cuts at: 104 104
Size: 4556
PvuII CAG'CTG
Cuts at: 227 1981 2341 227
Size: 1754 360 2442
Fragments arranged by size:
2442 1754 360
RsrII CG'GwC_CG
Cuts at: 2751 2751
Size: 4556
SacI G_AGCT'C
Cuts at: 1230 1570 1230
Size: 340 4216
SalI G'TCGA_C
Cuts at: 1593 1593
Size: 4556
SapI GCTCTTCn'nnn_
Cuts at: 2579 2789 2579
Size: 210 4346
SbfI CC_TGCA'GG
Cuts at: 800 1603 800
Size: 803 3753
ScaI AGT'ACT
Cuts at: 4073 4073
Size: 4556
SfcI C'TryA_G
Cuts at: 33 416 796 1287 1599 2284 3277 3468 33
Size: 383 380 491 312 685 993 191 1121
Fragments arranged by size:
1121 993 685 491 383 380 312 191
SfoI GGC'GCC
Cuts at: 1988 2236 1988
Size: 248 4308
SgfI GCG_AT'CGC
Cuts at: 104 104
Size: 4556
SmaI CCC'GGG
Cuts at: 721 1578 721
Size: 857 3699
SmlI C'TyrA_G
Cuts at: 1711 2626 3118 3380 1711
Size: 915 492 262 2887
Fragments arranged by size:
2887 915 492 262
SpeI A'CTAG_T
Cuts at: 67 67
Size: 4556
SphI G_CATG'C
Cuts at: 1609 2109 1609
Size: 500 4056
StyI C'CwwG_G
Cuts at: 138 311 1427 1690 2044 138
Size: 173 1116 263 354 2650
Fragments arranged by size:
2650 1116 354 263 173
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 1407 2139 2272 2731 2940 1407
Size: 732 133 459 209 3023
Fragments arranged by size:
3023 732 459 209 133
TatI w'GTAC_w
Cuts at: 986 4071 986
Size: 3085 1471
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 605 637 997 1029 1112 1144 4073 4105 605
Size: 32 360 32 83 32 2929 32 1056
Fragments arranged by size:
2929 1056 360 83 32 32 32 32
Tth111I GACn'n_nGTC
Cuts at: 1078 2353 1078
Size: 1275 3281
XbaI T'CTAG_A
Cuts at: 1587 1587
Size: 4556
XcmI CCAnnnn_n'nnnnTGG
Cuts at: 441 504 744 951 1344 2051 441
Size: 63 240 207 393 707 2946
Fragments arranged by size:
2946 707 393 240 207 63
XmaI C'CCGG_G
Cuts at: 719 1576 719
Size: 857 3699
Enzymes that do cut:
AarI AccI Acc65I AclI AcuI AflIII AgeI AleI
AloI AlwNI ApaI ApaLI ApoI AseI AsiSI AvaI
BamHI BanI BanII BbeI BbvCI BciVI BclI BglII
BlpI Bme1580I BmrI BplI BpmI Bpu10I BpuEI BsaI
BsaAI BsaBI BsaHI BsaWI BsaXI BseRI BseYI BsgI
BsiEI BsiHKAI BsmBI Bsp1286I BspEI BspHI BspMI BsrBI
BsrDI BsrFI BssSI BstAPI BstBI BstXI BstYI Bsu36I
BtgI BtgZI BtsI DraI DrdI EaeI EagI EarI
EciI EcoICRI Eco57MI EcoO109I EcoRI EcoRV FalI FspI
HaeII Hin4I HincII KasI KpnI MluI MmeI MscI
MslI MspA1I NaeI NarI NgoMIV NspI PasI PciI
PflMI PmeI PpiI PpuMI PspOMI PstI PvuI PvuII
RsrII SacI SalI SapI SbfI ScaI SfcI SfoI
SgfI SmaI SmlI SpeI SphI StyI TaqII TatI
TstI Tth111I XbaI XcmI XmaI
Enzymes that do not cut:
AatII AfeI AflII AhdI AlfI AscI AvrII BaeI
BbsI BcgI BfrBI BglI BmgBI BmtI BsiWI BsmI
BsrGI BssHII BstEII BstZ17I ClaI CspCI DraIII EcoNI
FseI FspAI HindIII HpaI MfeI NcoI NdeI NheI
NotI NruI NsiI PacI PfoI PmlI PshAI PsiI
PspXI PsrI SacII SanDI SexAI SfiI SgrAI SnaBI
SrfI SspI StuI SwaI XhoI XmnI ZraI