Restriction map for
pF1KB7250
(Circular) (Six-base) MAPSORT of: pF1KB7250.seq Check: 527 from: 1 to: 3557
pF1KB7250 3557 bp
With 182 enzymes: SgfI *
June 20, 2008 10:26 ..
AarI CACCTGCnnnn'nnnn_
Cuts at: 457 457
Size: 3557
AccI GT'mk_AC
Cuts at: 595 595
Size: 3557
Acc65I G'GTAC_C
Cuts at: 573 573
Size: 3557
AclI AA'CG_TT
Cuts at: 3397 3397
Size: 3557
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 1382 1814 2561 2829 1382
Size: 432 747 268 2110
Fragments arranged by size:
2110 747 432 268
AflIII A'CryG_T
Cuts at: 909 2013 2990 909
Size: 1104 977 1476
Fragments arranged by size:
1476 1104 977
AgeI A'CCGG_T
Cuts at: 1945 1945
Size: 3557
AhdI GACnn_n'nnGTC
Cuts at: 280 280
Size: 3557
AlwNI CAG_nnn'CTG
Cuts at: 423 520 862 2429 423
Size: 97 342 1567 1551
Fragments arranged by size:
1567 1551 342 97
ApaI G_GGCC'C
Cuts at: 303 382 400 303
Size: 79 18 3460
Fragments arranged by size:
3460 79 18
ApaLI G'TGCA_C
Cuts at: 2327 2327
Size: 3557
ApoI r'AATT_y
Cuts at: 561 2910 3166 561
Size: 2349 256 952
Fragments arranged by size:
2349 952 256
AseI AT'TA_AT
Cuts at: 20 20
Size: 3557
AsiSI GCG_AT'CGC
Cuts at: 104 104
Size: 3557
AvaI C'yCGr_G
Cuts at: 375 393 577 375
Size: 18 184 3355
Fragments arranged by size:
3355 184 18
BamHI G'GATC_C
Cuts at: 116 582 116
Size: 466 3091
BanI G'GyrC_C
Cuts at: 260 319 445 573 987 1235 1270 260
Size: 59 126 128 414 248 35 2547
Fragments arranged by size:
2547 414 248 128 126 59 35
BanII G_rGCy'C
Cuts at: 224 238 303 382 400 571 1601 224
Size: 14 65 79 18 171 1030 2180
Fragments arranged by size:
2180 1030 171 79 65 18 14
BbeI G_GCGC'C
Cuts at: 991 1239 991
Size: 248 3309
BbsI GAAGACnn'nnnn_
Cuts at: 393 393
Size: 3557
BciVI GTATCCnnnnn_n'
Cuts at: 1455 2216 3557 1455
Size: 761 1341 1455
Fragments arranged by size:
1455 1341 761
BclI T'GATC_A
Cuts at: 1077 1077
Size: 3557
BglI GCCn_nnn'nGGC
Cuts at: 307 328 307
Size: 21 3536
BglII A'GATC_T
Cuts at: 1072 3109 1072
Size: 2037 1520
BlpI GC'TnA_GC
Cuts at: 669 669
Size: 3557
Bme1580I G_kGCm'C
Cuts at: 303 382 400 448 1182 1275 2331 303
Size: 79 18 48 734 93 1056 1529
Fragments arranged by size:
1529 1056 734 93 79 48 18
BmrI ACTGGGnnnn_n'
Cuts at: 769 850 1185 3329 769
Size: 81 335 2144 997
Fragments arranged by size:
2144 997 335 81
BplI GAGnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 1924 1924
Size: 3557
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 289 289
Size: 3557
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 733 1612 2104 2402 733
Size: 879 492 298 1888
Fragments arranged by size:
1888 879 492 298
BsaI GGTCTCn'nnnn_
Cuts at: 3261 3261
Size: 3557
BsaAI yAC'GTr
Cuts at: 910 910
Size: 3557
BsaBI GATnn'nnATC
Cuts at: 6 1071 3108 6
Size: 1065 2037 455
Fragments arranged by size:
2037 1065 455
BsaHI Gr'CG_yC
Cuts at: 988 1236 1938 3434 988
Size: 248 702 1496 1111
Fragments arranged by size:
1496 1111 702 248
BsaWI w'CCGG_w
Cuts at: 746 1267 1945 2219 2366 2777 746
Size: 521 678 274 147 411 1526
Fragments arranged by size:
1526 678 521 411 274 147
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 139 139
Size: 3557
BseYI C'CCAG_C
Cuts at: 225 416 441 529 982 1740 2317 2813 225
Size: 191 25 88 453 758 577 496 969
Fragments arranged by size:
969 758 577 496 453 191 88 25
BsgI GTGCAGnnnnnnnnnnnnnn_nn'
Cuts at: 478 478
Size: 3557
BsiEI CG_ry'CG
Cuts at: 104 128 500 504 1099 1145 2353 104
Size: 24 372 4 595 46 1208 1308
Fragments arranged by size:
1308 1208 595 372 46 24 4
BsiHKAI G_wGCw'C
Cuts at: 571 1349 1539 2331 571
Size: 778 190 792 1797
Fragments arranged by size:
1797 792 778 190
Bsp1286I G_dGCh'C
Cuts at: 224 238 303 382 400 448 571 1182 1275
Size: 14 65 79 18 48 123 611 93
Cuts at: 1275 1349 1539 1601 2331 224
Size: 74 190 62 730 1450
Fragments arranged by size:
1450 730 611 190 123 93 79 74
65 62 48 18 14
BspEI T'CCGG_A
Cuts at: 2777 2777
Size: 3557
BspHI T'CATG_A
Cuts at: 2733 2733
Size: 3557
BspMI ACCTGCnnnn'nnnn_
Cuts at: 457 607 821 1123 1504 457
Size: 150 214 302 381 2510
Fragments arranged by size:
2510 381 302 214 150
BsrBI CCG'CTC
Cuts at: 50 1849 1903 3387 3552 50
Size: 1799 54 1484 165 55
Fragments arranged by size:
1799 1484 165 55 54
BsrDI GCAATG_nn'
Cuts at: 1469 1469
Size: 3557
BsrFI r'CCGG_y
Cuts at: 322 495 499 503 1555 1736 1945 322
Size: 173 4 4 1052 181 209 1934
Fragments arranged by size:
1934 1052 209 181 173 4 4
BssSI C'ACGA_G
Cuts at: 1828 2186 1828
Size: 358 3199
BstAPI GCAn_nnn'nTGC
Cuts at: 934 934
Size: 3557
BstBI TT'CG_AA
Cuts at: 1918 1918
Size: 3557
BstXI CCAn_nnnn'nTGG
Cuts at: 547 547
Size: 3557
BstYI r'GATC_y
Cuts at: 1 116 582 1049 1072 1407 1653 2665 2751
Size: 115 466 467 23 335 246 1012 86
Cuts at: 2751 2763 3109 1
Size: 12 346 449
Fragments arranged by size:
1012 467 466 449 346 335 246 115
86 23 12
BtgI C'CryG_G
Cuts at: 107 107
Size: 3557
BtgZI GCGATGnnnnnnnnnn'nnnn_
Cuts at: 1507 1688 1851 1507
Size: 181 163 3213
Fragments arranged by size:
3213 181 163
BtsI GCAGTG_nn'
Cuts at: 800 800
Size: 3557
DraI TTT'AAA
Cuts at: 556 556
Size: 3557
DrdI GACnn_nn'nnGTC
Cuts at: 1263 2121 1263
Size: 858 2699
EaeI y'GGCC_r
Cuts at: 125 363 497 501 1142 1316 1707 1734 3079
Size: 238 134 4 641 174 391 27 1345
Cuts at: 3079 125
Size: 603
Fragments arranged by size:
1345 641 603 391 238 174 134 27
4
EagI C'GGCC_G
Cuts at: 125 497 501 1142 125
Size: 372 4 641 2540
Fragments arranged by size:
2540 641 372 4
EarI CTCTTCn'nnn_
Cuts at: 1580 1790 1580
Size: 210 3347
EciI GGCGGAnnnnnnnnn_nn'
Cuts at: 1765 2075 2221 3367 1765
Size: 310 146 1146 1955
Fragments arranged by size:
1955 1146 310 146
EcoICRI GAG'CTC
Cuts at: 569 569
Size: 3557
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 289 1382 1814 2561 2829 289
Size: 1093 432 747 268 1017
Fragments arranged by size:
1093 1017 747 432 268
EcoO109I rG'GnC_Cy
Cuts at: 195 300 379 397 407 696 195
Size: 105 79 18 10 289 3056
Fragments arranged by size:
3056 289 105 79 18 10
EcoRI G'AATT_C
Cuts at: 561 561
Size: 3557
FalI AAGnnnnnCTTnnnnnnnn_nnnnn'
Cuts at: 1576 1576
Size: 3557
FseI GG_CCGG'CC
Cuts at: 503 503
Size: 3557
FspI TGC'GCA
Cuts at: 1338 1338
Size: 3557
HaeII r_GCGC'y
Cuts at: 991 1239 2261 3240 991
Size: 248 1022 979 1308
Fragments arranged by size:
1308 1022 979 248
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 266 298 1399 1429 1431 1461 266
Size: 32 1101 30 2 30 2362
Fragments arranged by size:
2362 1101 32 30 30 2
HincII GTy'rAC
Cuts at: 596 596
Size: 3557
HindIII A'AGCT_T
Cuts at: 289 289
Size: 3557
KasI G'GCGC_C
Cuts at: 987 1235 987
Size: 248 3309
KpnI G_GTAC'C
Cuts at: 577 577
Size: 3557
MluI A'CGCG_T
Cuts at: 2990 2990
Size: 3557
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 2228 2412 2228
Size: 184 3373
MscI TGG'CCA
Cuts at: 1318 1318
Size: 3557
MslI CAynn'nnrTG
Cuts at: 545 1546 1673 545
Size: 1001 127 2429
Fragments arranged by size:
2429 1001 127
MspA1I CmG'CkG
Cuts at: 668 982 1342 2355 2600 668
Size: 314 360 1013 245 1625
Fragments arranged by size:
1625 1013 360 314 245
NaeI GCC'GGC
Cuts at: 324 501 505 1738 324
Size: 177 4 1233 2143
Fragments arranged by size:
2143 1233 177 4
NarI GG'CG_CC
Cuts at: 988 1236 988
Size: 248 3309
NcoI C'CATG_G
Cuts at: 107 107
Size: 3557
NgoMIV G'CCGG_C
Cuts at: 322 499 503 1736 322
Size: 177 4 1233 2143
Fragments arranged by size:
2143 1233 177 4
NspI r_CATG'y
Cuts at: 610 1110 2017 610
Size: 500 907 2150
Fragments arranged by size:
2150 907 500
PasI CC'CwG_GG
Cuts at: 132 215 132
Size: 83 3474
PciI A'CATG_T
Cuts at: 2013 2013
Size: 3557
PfoI T'CCnGG_A
Cuts at: 353 353
Size: 3557
PmeI GTTT'AAAC
Cuts at: 556 556
Size: 3557
PpiI GAACnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 2696 2728 2696
Size: 32 3525
PpuMI rG'GwC_Cy
Cuts at: 195 407 195
Size: 212 3345
PspOMI G'GGCC_C
Cuts at: 299 378 396 299
Size: 79 18 3460
Fragments arranged by size:
3460 79 18
PstI C_TGCA'G
Cuts at: 161 254 604 1289 161
Size: 93 350 685 2429
Fragments arranged by size:
2429 685 350 93
PvuI CG_AT'CG
Cuts at: 104 104
Size: 3557
PvuII CAG'CTG
Cuts at: 982 1342 982
Size: 360 3197
RsrII CG'GwC_CG
Cuts at: 1752 1752
Size: 3557
SacI G_AGCT'C
Cuts at: 571 571
Size: 3557
SalI G'TCGA_C
Cuts at: 594 594
Size: 3557
SanDI GG'GwC_CC
Cuts at: 195 195
Size: 3557
SapI GCTCTTCn'nnn_
Cuts at: 1580 1790 1580
Size: 210 3347
SbfI CC_TGCA'GG
Cuts at: 604 604
Size: 3557
ScaI AGT'ACT
Cuts at: 3074 3074
Size: 3557
SfcI C'TryA_G
Cuts at: 33 157 250 600 1285 2278 2469 33
Size: 124 93 350 685 993 191 1121
Fragments arranged by size:
1121 993 685 350 191 124 93
SfoI GGC'GCC
Cuts at: 989 1237 989
Size: 248 3309
SgfI GCG_AT'CGC
Cuts at: 104 104
Size: 3557
SmaI CCC'GGG
Cuts at: 579 579
Size: 3557
SmlI C'TyrA_G
Cuts at: 712 1627 2119 2381 712
Size: 915 492 262 1888
Fragments arranged by size:
1888 915 492 262
SpeI A'CTAG_T
Cuts at: 67 67
Size: 3557
SphI G_CATG'C
Cuts at: 610 1110 610
Size: 500 3057
StyI C'CwwG_G
Cuts at: 107 691 1045 107
Size: 584 354 2619
Fragments arranged by size:
2619 584 354
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 1140 1273 1732 1941 1140
Size: 133 459 209 2756
Fragments arranged by size:
2756 459 209 133
TatI w'GTAC_w
Cuts at: 3072 3072
Size: 3557
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 3074 3106 3074
Size: 32 3525
Tth111I GACn'n_nGTC
Cuts at: 1354 1354
Size: 3557
XbaI T'CTAG_A
Cuts at: 588 588
Size: 3557
XcmI CCAnnnn_n'nnnnTGG
Cuts at: 1052 1052
Size: 3557
XmaI C'CCGG_G
Cuts at: 577 577
Size: 3557
Enzymes that do cut:
AarI AccI Acc65I AclI AcuI AflIII AgeI AhdI
AlwNI ApaI ApaLI ApoI AseI AsiSI AvaI BamHI
BanI BanII BbeI BbsI BciVI BclI BglI BglII
BlpI Bme1580I BmrI BplI BpmI BpuEI BsaI BsaAI
BsaBI BsaHI BsaWI BseRI BseYI BsgI BsiEI BsiHKAI
Bsp1286I BspEI BspHI BspMI BsrBI BsrDI BsrFI BssSI
BstAPI BstBI BstXI BstYI BtgI BtgZI BtsI DraI
DrdI EaeI EagI EarI EciI EcoICRI Eco57MI EcoO109I
EcoRI FalI FseI FspI HaeII Hin4I HincII HindIII
KasI KpnI MluI MmeI MscI MslI MspA1I NaeI
NarI NcoI NgoMIV NspI PasI PciI PfoI PmeI
PpiI PpuMI PspOMI PstI PvuI PvuII RsrII SacI
SalI SanDI SapI SbfI ScaI SfcI SfoI SgfI
SmaI SmlI SpeI SphI StyI TaqII TatI TstI
Tth111I XbaI XcmI XmaI
Enzymes that do not cut:
AatII AfeI AflII AleI AlfI AloI AscI AvrII
BaeI BbvCI BcgI BfrBI BmgBI BmtI Bpu10I BsaXI
BsiWI BsmI BsmBI BsrGI BssHII BstEII BstZ17I Bsu36I
ClaI CspCI DraIII EcoNI EcoRV FspAI HpaI MfeI
NdeI NheI NotI NruI NsiI PacI PflMI PmlI
PshAI PsiI PspXI PsrI SacII SexAI SfiI SgrAI
SnaBI SrfI SspI StuI SwaI XhoI XmnI ZraI