Restriction map for
pF1KB7249
(Circular) (Six-base) MAPSORT of: pF1KB7249.seq Check: 5576 from: 1 to: 3482
pF1KB7249 3482 bp
With 182 enzymes: SgfI *
June 20, 2008 10:26 ..
AccI GT'mk_AC
Cuts at: 520 520
Size: 3482
Acc65I G'GTAC_C
Cuts at: 498 498
Size: 3482
AclI AA'CG_TT
Cuts at: 3322 3322
Size: 3482
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 245 374 1307 1739 2486 2754 245
Size: 129 933 432 747 268 973
Fragments arranged by size:
973 933 747 432 268 129
AflIII A'CryG_T
Cuts at: 834 1938 2915 834
Size: 1104 977 1401
Fragments arranged by size:
1401 1104 977
AgeI A'CCGG_T
Cuts at: 1870 1870
Size: 3482
AlwNI CAG_nnn'CTG
Cuts at: 787 2354 787
Size: 1567 1915
ApaLI G'TGCA_C
Cuts at: 2252 2252
Size: 3482
ApoI r'AATT_y
Cuts at: 486 2835 3091 486
Size: 2349 256 877
Fragments arranged by size:
2349 877 256
AseI AT'TA_AT
Cuts at: 20 20
Size: 3482
AsiSI GCG_AT'CGC
Cuts at: 104 104
Size: 3482
AvaI C'yCGr_G
Cuts at: 502 502
Size: 3482
BamHI G'GATC_C
Cuts at: 507 507
Size: 3482
BanI G'GyrC_C
Cuts at: 498 912 1160 1195 498
Size: 414 248 35 2785
Fragments arranged by size:
2785 414 248 35
BanII G_rGCy'C
Cuts at: 496 1526 496
Size: 1030 2452
BbeI G_GCGC'C
Cuts at: 916 1164 916
Size: 248 3234
BciVI GTATCCnnnnn_n'
Cuts at: 1380 2141 3482 1380
Size: 761 1341 1380
Fragments arranged by size:
1380 1341 761
BclI T'GATC_A
Cuts at: 1002 1002
Size: 3482
BglII A'GATC_T
Cuts at: 997 3034 997
Size: 2037 1445
BlpI GC'TnA_GC
Cuts at: 594 594
Size: 3482
Bme1580I G_kGCm'C
Cuts at: 1107 1200 2256 1107
Size: 93 1056 2333
Fragments arranged by size:
2333 1056 93
BmrI ACTGGGnnnn_n'
Cuts at: 162 259 694 775 1110 3254 162
Size: 97 435 81 335 2144 390
Fragments arranged by size:
2144 435 390 335 97 81
BplI GAGnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 438 1849 438
Size: 1411 2071
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 139 139
Size: 3482
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 658 1537 2029 2327 658
Size: 879 492 298 1813
Fragments arranged by size:
1813 879 492 298
BsaI GGTCTCn'nnnn_
Cuts at: 3186 3186
Size: 3482
BsaAI yAC'GTr
Cuts at: 835 835
Size: 3482
BsaBI GATnn'nnATC
Cuts at: 6 996 3033 6
Size: 990 2037 455
Fragments arranged by size:
2037 990 455
BsaHI Gr'CG_yC
Cuts at: 913 1161 1863 3359 913
Size: 248 702 1496 1036
Fragments arranged by size:
1496 1036 702 248
BsaWI w'CCGG_w
Cuts at: 671 1192 1870 2144 2291 2702 671
Size: 521 678 274 147 411 1451
Fragments arranged by size:
1451 678 521 411 274 147
BseYI C'CCAG_C
Cuts at: 907 1665 2242 2738 907
Size: 758 577 496 1651
Fragments arranged by size:
1651 758 577 496
BsgI GTGCAGnnnnnnnnnnnnnn_nn'
Cuts at: 493 493
Size: 3482
BsiEI CG_ry'CG
Cuts at: 104 1024 1070 2278 104
Size: 920 46 1208 1308
Fragments arranged by size:
1308 1208 920 46
BsiHKAI G_wGCw'C
Cuts at: 496 1274 1464 2256 496
Size: 778 190 792 1722
Fragments arranged by size:
1722 792 778 190
Bsp1286I G_dGCh'C
Cuts at: 496 1107 1200 1274 1464 1526 2256 496
Size: 611 93 74 190 62 730 1722
Fragments arranged by size:
1722 730 611 190 93 74 62
BspEI T'CCGG_A
Cuts at: 2702 2702
Size: 3482
BspHI T'CATG_A
Cuts at: 2658 2658
Size: 3482
BspMI ACCTGCnnnn'nnnn_
Cuts at: 465 532 746 1048 1429 465
Size: 67 214 302 381 2518
Fragments arranged by size:
2518 381 302 214 67
BsrBI CCG'CTC
Cuts at: 50 1774 1828 3312 3477 50
Size: 1724 54 1484 165 55
Fragments arranged by size:
1724 1484 165 55 54
BsrDI GCAATG_nn'
Cuts at: 1394 1394
Size: 3482
BsrFI r'CCGG_y
Cuts at: 1480 1661 1870 1480
Size: 181 209 3092
Fragments arranged by size:
3092 209 181
BssSI C'ACGA_G
Cuts at: 1753 2111 1753
Size: 358 3124
BstAPI GCAn_nnn'nTGC
Cuts at: 859 859
Size: 3482
BstBI TT'CG_AA
Cuts at: 1843 1843
Size: 3482
BstYI r'GATC_y
Cuts at: 1 300 507 974 997 1332 1578 2590 2676
Size: 299 207 467 23 335 246 1012 86
Cuts at: 2676 2688 3034 1
Size: 12 346 449
Fragments arranged by size:
1012 467 449 346 335 299 246 207
86 23 12
BtgI C'CryG_G
Cuts at: 107 107
Size: 3482
BtgZI GCGATGnnnnnnnnnn'nnnn_
Cuts at: 1432 1613 1776 1432
Size: 181 163 3138
Fragments arranged by size:
3138 181 163
BtsI GCAGTG_nn'
Cuts at: 725 725
Size: 3482
DraI TTT'AAA
Cuts at: 481 481
Size: 3482
DrdI GACnn_nn'nnGTC
Cuts at: 1188 2046 1188
Size: 858 2624
EaeI y'GGCC_r
Cuts at: 1067 1241 1632 1659 3004 1067
Size: 174 391 27 1345 1545
Fragments arranged by size:
1545 1345 391 174 27
EagI C'GGCC_G
Cuts at: 1067 1067
Size: 3482
EarI CTCTTCn'nnn_
Cuts at: 1505 1715 1505
Size: 210 3272
EciI GGCGGAnnnnnnnnn_nn'
Cuts at: 1690 2000 2146 3292 1690
Size: 310 146 1146 1880
Fragments arranged by size:
1880 1146 310 146
EcoICRI GAG'CTC
Cuts at: 494 494
Size: 3482
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 139 245 374 1307 1739 2486 2754 139
Size: 106 129 933 432 747 268 867
Fragments arranged by size:
933 867 747 432 268 129 106
EcoNI CCTnn'n_nnAGG
Cuts at: 169 169
Size: 3482
EcoO109I rG'GnC_Cy
Cuts at: 621 621
Size: 3482
EcoRI G'AATT_C
Cuts at: 486 486
Size: 3482
EcoRV GAT'ATC
Cuts at: 330 330
Size: 3482
FalI AAGnnnnnCTTnnnnnnnn_nnnnn'
Cuts at: 396 1501 396
Size: 1105 2377
FspI TGC'GCA
Cuts at: 1263 1263
Size: 3482
HaeII r_GCGC'y
Cuts at: 916 1164 2186 3165 916
Size: 248 1022 979 1233
Fragments arranged by size:
1233 1022 979 248
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 1324 1354 1356 1386 1324
Size: 30 2 30 3420
Fragments arranged by size:
3420 30 30 2
HincII GTy'rAC
Cuts at: 521 521
Size: 3482
HindIII A'AGCT_T
Cuts at: 338 338
Size: 3482
KasI G'GCGC_C
Cuts at: 912 1160 912
Size: 248 3234
KpnI G_GTAC'C
Cuts at: 502 502
Size: 3482
MluI A'CGCG_T
Cuts at: 2915 2915
Size: 3482
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 2153 2337 2153
Size: 184 3298
MscI TGG'CCA
Cuts at: 1243 1243
Size: 3482
MslI CAynn'nnrTG
Cuts at: 1471 1598 1471
Size: 127 3355
MspA1I CmG'CkG
Cuts at: 459 593 907 1267 2280 2525 459
Size: 134 314 360 1013 245 1416
Fragments arranged by size:
1416 1013 360 314 245 134
NaeI GCC'GGC
Cuts at: 1663 1663
Size: 3482
NarI GG'CG_CC
Cuts at: 913 1161 913
Size: 248 3234
NcoI C'CATG_G
Cuts at: 107 107
Size: 3482
NgoMIV G'CCGG_C
Cuts at: 1661 1661
Size: 3482
NspI r_CATG'y
Cuts at: 535 1035 1942 535
Size: 500 907 2075
Fragments arranged by size:
2075 907 500
PciI A'CATG_T
Cuts at: 1938 1938
Size: 3482
PfoI T'CCnGG_A
Cuts at: 116 116
Size: 3482
PmeI GTTT'AAAC
Cuts at: 481 481
Size: 3482
PpiI GAACnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 2621 2653 2621
Size: 32 3450
PstI C_TGCA'G
Cuts at: 468 529 1214 468
Size: 61 685 2736
Fragments arranged by size:
2736 685 61
PvuI CG_AT'CG
Cuts at: 104 104
Size: 3482
PvuII CAG'CTG
Cuts at: 459 907 1267 459
Size: 448 360 2674
Fragments arranged by size:
2674 448 360
RsrII CG'GwC_CG
Cuts at: 1677 1677
Size: 3482
SacI G_AGCT'C
Cuts at: 496 496
Size: 3482
SalI G'TCGA_C
Cuts at: 519 519
Size: 3482
SapI GCTCTTCn'nnn_
Cuts at: 1505 1715 1505
Size: 210 3272
SbfI CC_TGCA'GG
Cuts at: 529 529
Size: 3482
ScaI AGT'ACT
Cuts at: 269 2999 269
Size: 2730 752
SfcI C'TryA_G
Cuts at: 33 464 525 1210 2203 2394 33
Size: 431 61 685 993 191 1121
Fragments arranged by size:
1121 993 685 431 191 61
SfoI GGC'GCC
Cuts at: 914 1162 914
Size: 248 3234
SgfI GCG_AT'CGC
Cuts at: 104 104
Size: 3482
SmaI CCC'GGG
Cuts at: 504 504
Size: 3482
SmlI C'TyrA_G
Cuts at: 637 1552 2044 2306 637
Size: 915 492 262 1813
Fragments arranged by size:
1813 915 492 262
SpeI A'CTAG_T
Cuts at: 67 67
Size: 3482
SphI G_CATG'C
Cuts at: 535 1035 535
Size: 500 2982
StyI C'CwwG_G
Cuts at: 107 170 616 970 107
Size: 63 446 354 2619
Fragments arranged by size:
2619 446 354 63
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 134 1065 1198 1657 1866 134
Size: 931 133 459 209 1750
Fragments arranged by size:
1750 931 459 209 133
TatI w'GTAC_w
Cuts at: 267 2997 267
Size: 2730 752
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 2999 3031 2999
Size: 32 3450
Tth111I GACn'n_nGTC
Cuts at: 1279 1279
Size: 3482
XbaI T'CTAG_A
Cuts at: 513 513
Size: 3482
XcmI CCAnnnn_n'nnnnTGG
Cuts at: 114 977 114
Size: 863 2619
XmaI C'CCGG_G
Cuts at: 502 502
Size: 3482
XmnI GAAnn'nnTTC
Cuts at: 376 376
Size: 3482
Enzymes that do cut:
AccI Acc65I AclI AcuI AflIII AgeI AlwNI ApaLI
ApoI AseI AsiSI AvaI BamHI BanI BanII BbeI
BciVI BclI BglII BlpI Bme1580I BmrI BplI BpmI
BpuEI BsaI BsaAI BsaBI BsaHI BsaWI BseYI BsgI
BsiEI BsiHKAI Bsp1286I BspEI BspHI BspMI BsrBI BsrDI
BsrFI BssSI BstAPI BstBI BstYI BtgI BtgZI BtsI
DraI DrdI EaeI EagI EarI EciI EcoICRI Eco57MI
EcoNI EcoO109I EcoRI EcoRV FalI FspI HaeII Hin4I
HincII HindIII KasI KpnI MluI MmeI MscI MslI
MspA1I NaeI NarI NcoI NgoMIV NspI PciI PfoI
PmeI PpiI PstI PvuI PvuII RsrII SacI SalI
SapI SbfI ScaI SfcI SfoI SgfI SmaI SmlI
SpeI SphI StyI TaqII TatI TstI Tth111I XbaI
XcmI XmaI XmnI
Enzymes that do not cut:
AarI AatII AfeI AflII AhdI AleI AlfI AloI
ApaI AscI AvrII BaeI BbsI BbvCI BcgI BfrBI
BglI BmgBI BmtI Bpu10I BsaXI BseRI BsiWI BsmI
BsmBI BsrGI BssHII BstEII BstXI BstZ17I Bsu36I ClaI
CspCI DraIII FseI FspAI HpaI MfeI NdeI NheI
NotI NruI NsiI PacI PasI PflMI PmlI PpuMI
PshAI PsiI PspOMI PspXI PsrI SacII SanDI SexAI
SfiI SgrAI SnaBI SrfI SspI StuI SwaI XhoI
ZraI