Restriction map for
pF1KB7247
(Circular) (Six-base) MAPSORT of: pF1KB7247.seq Check: 9396 from: 1 to: 4901
pF1KB7247 4901 bp
With 182 enzymes: SgfI *
June 20, 2008 10:26 ..
AarI CACCTGCnnnn'nnnn_
Cuts at: 1115 1147 1115
Size: 32 4869
AccI GT'mk_AC
Cuts at: 1883 1939 1883
Size: 56 4845
Acc65I G'GTAC_C
Cuts at: 1917 1917
Size: 4901
AclI AA'CG_TT
Cuts at: 4741 4741
Size: 4901
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 248 511 978 2726 3158 3905 4173 248
Size: 263 467 1748 432 747 268 976
Fragments arranged by size:
1748 976 747 467 432 268 263
AflIII A'CryG_T
Cuts at: 467 2253 3357 4334 467
Size: 1786 1104 977 1034
Fragments arranged by size:
1786 1104 1034 977
AgeI A'CCGG_T
Cuts at: 3289 3289
Size: 4901
AleI CACnn'nnGTG
Cuts at: 1076 1076
Size: 4901
AlfI GCAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 1179 1179
Size: 4901
AlwNI CAG_nnn'CTG
Cuts at: 1623 2206 3773 1623
Size: 583 1567 2751
Fragments arranged by size:
2751 1567 583
ApaI G_GGCC'C
Cuts at: 584 584
Size: 4901
ApaLI G'TGCA_C
Cuts at: 3671 3671
Size: 4901
ApoI r'AATT_y
Cuts at: 1905 4254 4510 1905
Size: 2349 256 2296
Fragments arranged by size:
2349 2296 256
AseI AT'TA_AT
Cuts at: 20 20
Size: 4901
AsiSI GCG_AT'CGC
Cuts at: 104 104
Size: 4901
AvaI C'yCGr_G
Cuts at: 122 378 1552 1921 122
Size: 256 1174 369 3102
Fragments arranged by size:
3102 1174 369 256
BamHI G'GATC_C
Cuts at: 473 1926 473
Size: 1453 3448
BanI G'GyrC_C
Cuts at: 415 1065 1078 1529 1917 2331 2579 2614 415
Size: 650 13 451 388 414 248 35 2702
Fragments arranged by size:
2702 650 451 414 388 248 35 13
BanII G_rGCy'C
Cuts at: 123 386 584 1915 2945 123
Size: 263 198 1331 1030 2079
Fragments arranged by size:
2079 1331 1030 263 198
BbeI G_GCGC'C
Cuts at: 419 1533 2335 2583 419
Size: 1114 802 248 2737
Fragments arranged by size:
2737 1114 802 248
BbsI GAAGACnn'nnnn_
Cuts at: 690 711 1836 690
Size: 21 1125 3755
Fragments arranged by size:
3755 1125 21
BciVI GTATCCnnnnn_n'
Cuts at: 2799 3560 4901 2799
Size: 761 1341 2799
Fragments arranged by size:
2799 1341 761
BclI T'GATC_A
Cuts at: 2421 2421
Size: 4901
BglI GCCn_nnn'nGGC
Cuts at: 127 361 1283 1388 1420 1639 127
Size: 234 922 105 32 219 3389
Fragments arranged by size:
3389 922 234 219 105 32
BglII A'GATC_T
Cuts at: 1694 2416 4453 1694
Size: 722 2037 2142
Fragments arranged by size:
2142 2037 722
BlpI GC'TnA_GC
Cuts at: 195 881 1580 2013 195
Size: 686 699 433 3083
Fragments arranged by size:
3083 699 686 433
Bme1580I G_kGCm'C
Cuts at: 584 1083 1288 1686 2526 2619 3675 584
Size: 499 205 398 840 93 1056 1810
Fragments arranged by size:
1810 1056 840 499 398 205 93
BmgBI CAC'GTC
Cuts at: 468 468
Size: 4901
BmrI ACTGGGnnnn_n'
Cuts at: 1387 2113 2194 2529 4673 1387
Size: 726 81 335 2144 1615
Fragments arranged by size:
2144 1615 726 335 81
BplI GAGnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 3268 3268
Size: 4901
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 1324 1627 1324
Size: 303 4598
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 1672 2077 2956 3448 3746 1672
Size: 405 879 492 298 2827
Fragments arranged by size:
2827 879 492 405 298
BsaI GGTCTCn'nnnn_
Cuts at: 177 913 4605 177
Size: 736 3692 473
Fragments arranged by size:
3692 736 473
BsaAI yAC'GTr
Cuts at: 1062 2254 1062
Size: 1192 3709
BsaBI GATnn'nnATC
Cuts at: 6 1390 2415 4452 6
Size: 1384 1025 2037 455
Fragments arranged by size:
2037 1384 1025 455
BsaHI Gr'CG_yC
Cuts at: 157 416 728 1530 2332 2580 3282 4778 157
Size: 259 312 802 802 248 702 1496 280
Fragments arranged by size:
1496 802 802 702 312 280 259 248
BsaWI w'CCGG_w
Cuts at: 965 2090 2611 3289 3563 3710 4121 965
Size: 1125 521 678 274 147 411 1745
Fragments arranged by size:
1745 1125 678 521 411 274 147
BsaXI ACnnnnnCTCCnnnnnnn_nnn'
Cuts at: 1636 1666 1859 1889 1636
Size: 30 193 30 4648
Fragments arranged by size:
4648 193 30 30
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 286 1321 286
Size: 1035 3866
BseYI C'CCAG_C
Cuts at: 146 1134 1611 2326 3084 3661 4157 146
Size: 988 477 715 758 577 496 890
Fragments arranged by size:
988 890 758 715 577 496 477
BsgI GTGCAGnnnnnnnnnnnnnn_nn'
Cuts at: 606 1093 1358 1507 606
Size: 487 265 149 4000
Fragments arranged by size:
4000 487 265 149
BsiEI CG_ry'CG
Cuts at: 104 284 2443 2489 3697 104
Size: 180 2159 46 1208 1308
Fragments arranged by size:
2159 1308 1208 180 46
BsiHKAI G_wGCw'C
Cuts at: 560 611 746 1178 1915 2693 2883 3675 560
Size: 51 135 432 737 778 190 792 1786
Fragments arranged by size:
1786 792 778 737 432 190 135 51
Bsp1286I G_dGCh'C
Cuts at: 123 386 560 584 611 746 1083 1178 1288
Size: 263 174 24 27 135 337 95 110
Cuts at: 1288 1686 1915 2526 2619 2693 2883 2945 3675
Size: 398 229 611 93 74 190 62 730
Cuts at: 3675 123
Size: 1349
Fragments arranged by size:
1349 730 611 398 337 263 229 190
174 135 110 95 93 74 62 27
24
BspEI T'CCGG_A
Cuts at: 965 4121 965
Size: 3156 1745
BspHI T'CATG_A
Cuts at: 4077 4077
Size: 4901
BspMI ACCTGCnnnn'nnnn_
Cuts at: 833 1115 1147 1951 2165 2467 2848 833
Size: 282 32 804 214 302 381 2886
Fragments arranged by size:
2886 804 381 302 282 214 32
BsrBI CCG'CTC
Cuts at: 50 127 194 280 3193 3247 4731 4896 50
Size: 77 67 86 2913 54 1484 165 55
Fragments arranged by size:
2913 1484 165 86 77 67 55 54
BsrDI GCAATG_nn'
Cuts at: 921 1205 2813 921
Size: 284 1608 3009
Fragments arranged by size:
3009 1608 284
BsrFI r'CCGG_y
Cuts at: 347 1526 2899 3080 3289 347
Size: 1179 1373 181 209 1959
Fragments arranged by size:
1959 1373 1179 209 181
BssSI C'ACGA_G
Cuts at: 3172 3530 3172
Size: 358 4543
BstAPI GCAn_nnn'nTGC
Cuts at: 629 1440 2278 629
Size: 811 838 3252
Fragments arranged by size:
3252 838 811
BstBI TT'CG_AA
Cuts at: 3262 3262
Size: 4901
BstXI CCAn_nnnn'nTGG
Cuts at: 364 1269 364
Size: 905 3996
BstYI r'GATC_y
Cuts at: 1 473 567 1694 1926 2393 2416 2751 2997
Size: 472 94 1127 232 467 23 335 246
Cuts at: 2997 4009 4095 4107 4453 1
Size: 1012 86 12 346 449
Fragments arranged by size:
1127 1012 472 467 449 346 335 246
232 94 86 23 12
BtgI C'CryG_G
Cuts at: 107 114 107
Size: 7 4894
BtgZI GCGATGnnnnnnnnnn'nnnn_
Cuts at: 1377 1476 2851 3032 3195 1377
Size: 99 1375 181 163 3083
Fragments arranged by size:
3083 1375 181 163 99
BtsI GCAGTG_nn'
Cuts at: 753 2144 753
Size: 1391 3510
DraI TTT'AAA
Cuts at: 1900 1900
Size: 4901
DraIII CAC_nnn'GTG
Cuts at: 555 794 953 1730 1863 555
Size: 239 159 777 133 3593
Fragments arranged by size:
3593 777 239 159 133
DrdI GACnn_nn'nnGTC
Cuts at: 2607 3465 2607
Size: 858 4043
EaeI y'GGCC_r
Cuts at: 281 449 482 815 842 1412 1421 2486 2660
Size: 168 33 333 27 570 9 1065 174
Cuts at: 2660 3051 3078 4423 281
Size: 391 27 1345 759
Fragments arranged by size:
1345 1065 759 570 391 333 174 168
33 27 27 9
EagI C'GGCC_G
Cuts at: 281 2486 281
Size: 2205 2696
EarI CTCTTCn'nnn_
Cuts at: 379 616 1183 1510 1708 2924 3134 379
Size: 237 567 327 198 1216 210 2146
Fragments arranged by size:
2146 1216 567 327 237 210 198
EciI GGCGGAnnnnnnnnn_nn'
Cuts at: 3109 3419 3565 4711 3109
Size: 310 146 1146 3299
Fragments arranged by size:
3299 1146 310 146
EcoICRI GAG'CTC
Cuts at: 1913 1913
Size: 4901
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 248 511 978 1324 1627 2726 3158 3905 4173
Size: 263 467 346 303 1099 432 747 268
Cuts at: 4173 248
Size: 976
Fragments arranged by size:
1099 976 747 467 432 346 303 268
263
EcoNI CCTnn'n_nnAGG
Cuts at: 1876 1876
Size: 4901
EcoO109I rG'GnC_Cy
Cuts at: 495 580 581 1518 1557 1620 2040 495
Size: 85 1 937 39 63 420 3356
Fragments arranged by size:
3356 937 420 85 63 39 1
EcoRI G'AATT_C
Cuts at: 1905 1905
Size: 4901
FalI AAGnnnnnCTTnnnnnnnn_nnnnn'
Cuts at: 901 2920 901
Size: 2019 2882
FspI TGC'GCA
Cuts at: 137 2682 137
Size: 2545 2356
HaeII r_GCGC'y
Cuts at: 202 419 1431 1533 2335 2583 3605 4584 202
Size: 217 1012 102 802 248 1022 979 519
Fragments arranged by size:
1022 1012 979 802 519 248 217 102
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 173 205 2743 2773 2775 2805 173
Size: 32 2538 30 2 30 2269
Fragments arranged by size:
2538 2269 32 30 30 2
HincII GTy'rAC
Cuts at: 1821 1940 1821
Size: 119 4782
HindIII A'AGCT_T
Cuts at: 959 959
Size: 4901
KasI G'GCGC_C
Cuts at: 415 1529 2331 2579 415
Size: 1114 802 248 2737
Fragments arranged by size:
2737 1114 802 248
KpnI G_GTAC'C
Cuts at: 1921 1921
Size: 4901
MluI A'CGCG_T
Cuts at: 4334 4334
Size: 4901
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 3572 3756 3572
Size: 184 4717
MscI TGG'CCA
Cuts at: 484 817 1414 1423 2662 484
Size: 333 597 9 1239 2723
Fragments arranged by size:
2723 1239 597 333 9
MslI CAynn'nnrTG
Cuts at: 712 895 1076 2890 3017 712
Size: 183 181 1814 127 2596
Fragments arranged by size:
2596 1814 183 181 127
MspA1I CmG'CkG
Cuts at: 116 146 620 674 687 1131 1629 2012 2326
Size: 30 474 54 13 444 498 383 314
Cuts at: 2326 2686 3699 3944 116
Size: 360 1013 245 1073
Fragments arranged by size:
1073 1013 498 474 444 383 360 314
245 54 30 13
NaeI GCC'GGC
Cuts at: 3082 3082
Size: 4901
NarI GG'CG_CC
Cuts at: 416 1530 2332 2580 416
Size: 1114 802 248 2737
Fragments arranged by size:
2737 1114 802 248
NcoI C'CATG_G
Cuts at: 107 107
Size: 4901
NgoMIV G'CCGG_C
Cuts at: 3080 3080
Size: 4901
NotI GC'GGCC_GC
Cuts at: 281 281
Size: 4901
NspI r_CATG'y
Cuts at: 1857 1954 2454 3361 1857
Size: 97 500 907 3397
Fragments arranged by size:
3397 907 500 97
PciI A'CATG_T
Cuts at: 3357 3357
Size: 4901
PmeI GTTT'AAAC
Cuts at: 1900 1900
Size: 4901
PmlI CAC'GTG
Cuts at: 1062 1062
Size: 4901
PpiI GAACnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 4040 4072 4040
Size: 32 4869
PpuMI rG'GwC_Cy
Cuts at: 495 495
Size: 4901
PspOMI G'GGCC_C
Cuts at: 580 580
Size: 4901
PstI C_TGCA'G
Cuts at: 430 1487 1628 1948 2633 430
Size: 1057 141 320 685 2698
Fragments arranged by size:
2698 1057 685 320 141
PvuI CG_AT'CG
Cuts at: 104 104
Size: 4901
PvuII CAG'CTG
Cuts at: 620 687 2326 2686 620
Size: 67 1639 360 2835
Fragments arranged by size:
2835 1639 360 67
RsrII CG'GwC_CG
Cuts at: 3096 3096
Size: 4901
SacI G_AGCT'C
Cuts at: 1915 1915
Size: 4901
SacII CC_GC'GG
Cuts at: 117 117
Size: 4901
SalI G'TCGA_C
Cuts at: 1938 1938
Size: 4901
SapI GCTCTTCn'nnn_
Cuts at: 616 1183 2924 3134 616
Size: 567 1741 210 2383
Fragments arranged by size:
2383 1741 567 210
SbfI CC_TGCA'GG
Cuts at: 1948 1948
Size: 4901
ScaI AGT'ACT
Cuts at: 1006 1219 4418 1006
Size: 213 3199 1489
Fragments arranged by size:
3199 1489 213
SfcI C'TryA_G
Cuts at: 33 426 1483 1624 1656 1944 2629 3622 3813
Size: 393 1057 141 32 288 685 993 191
Cuts at: 3813 33
Size: 1121
Fragments arranged by size:
1121 1057 993 685 393 288 191 141
32
SfiI GGCCn_nnn'nGGCC
Cuts at: 1420 1639 1420
Size: 219 4682
SfoI GGC'GCC
Cuts at: 417 1531 2333 2581 417
Size: 1114 802 248 2737
Fragments arranged by size:
2737 1114 802 248
SgfI GCG_AT'CGC
Cuts at: 104 104
Size: 4901
SgrAI Cr'CCGG_yG
Cuts at: 1526 1526
Size: 4901
SmaI CCC'GGG
Cuts at: 1923 1923
Size: 4901
SmlI C'TyrA_G
Cuts at: 1687 2056 2971 3463 3725 1687
Size: 369 915 492 262 2863
Fragments arranged by size:
2863 915 492 369 262
SpeI A'CTAG_T
Cuts at: 67 67
Size: 4901
SphI G_CATG'C
Cuts at: 1857 1954 2454 1857
Size: 97 500 4304
Fragments arranged by size:
4304 500 97
StuI AGG'CCT
Cuts at: 1871 1871
Size: 4901
StyI C'CwwG_G
Cuts at: 107 2035 2389 107
Size: 1928 354 2619
Fragments arranged by size:
2619 1928 354
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 159 400 451 579 729 1066 1357 2484 2617
Size: 241 51 128 150 337 291 1127 133
Cuts at: 2617 3076 3285 159
Size: 459 209 1775
Fragments arranged by size:
1775 1127 459 337 291 241 209 150
133 128 51
TatI w'GTAC_w
Cuts at: 1004 1217 4416 1004
Size: 213 3199 1489
Fragments arranged by size:
3199 1489 213
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 1859 1891 4418 4450 1859
Size: 32 2527 32 2310
Fragments arranged by size:
2527 2310 32 32
Tth111I GACn'n_nGTC
Cuts at: 694 1324 2698 694
Size: 630 1374 2897
Fragments arranged by size:
2897 1374 630
XbaI T'CTAG_A
Cuts at: 1932 1932
Size: 4901
XcmI CCAnnnn_n'nnnnTGG
Cuts at: 1266 1299 2396 1266
Size: 33 1097 3771
Fragments arranged by size:
3771 1097 33
XmaI C'CCGG_G
Cuts at: 1921 1921
Size: 4901
XmnI GAAnn'nnTTC
Cuts at: 1504 1504
Size: 4901
Enzymes that do cut:
AarI AccI Acc65I AclI AcuI AflIII AgeI AleI
AlfI AlwNI ApaI ApaLI ApoI AseI AsiSI AvaI
BamHI BanI BanII BbeI BbsI BciVI BclI BglI
BglII BlpI Bme1580I BmgBI BmrI BplI BpmI BpuEI
BsaI BsaAI BsaBI BsaHI BsaWI BsaXI BseRI BseYI
BsgI BsiEI BsiHKAI Bsp1286I BspEI BspHI BspMI BsrBI
BsrDI BsrFI BssSI BstAPI BstBI BstXI BstYI BtgI
BtgZI BtsI DraI DraIII DrdI EaeI EagI EarI
EciI EcoICRI Eco57MI EcoNI EcoO109I EcoRI FalI FspI
HaeII Hin4I HincII HindIII KasI KpnI MluI MmeI
MscI MslI MspA1I NaeI NarI NcoI NgoMIV NotI
NspI PciI PmeI PmlI PpiI PpuMI PspOMI PstI
PvuI PvuII RsrII SacI SacII SalI SapI SbfI
ScaI SfcI SfiI SfoI SgfI SgrAI SmaI SmlI
SpeI SphI StuI StyI TaqII TatI TstI Tth111I
XbaI XcmI XmaI XmnI
Enzymes that do not cut:
AatII AfeI AflII AhdI AloI AscI AvrII BaeI
BbvCI BcgI BfrBI BmtI Bpu10I BsiWI BsmI BsmBI
BsrGI BssHII BstEII BstZ17I Bsu36I ClaI CspCI EcoRV
FseI FspAI HpaI MfeI NdeI NheI NruI NsiI
PacI PasI PflMI PfoI PshAI PsiI PspXI PsrI
SanDI SexAI SnaBI SrfI SspI SwaI XhoI ZraI